The cytochrome c gene from the green alga Chlamydomonas reinhardtii. Structure and expression in wild-type cells and in obligate photoautotrophic (dk) mutants

Autores
Felitti, Silvina Andrea; Chan, Raquel Lia; Sierra, Manuel González; Gonzalez, Daniel Hector
Año de publicación
2000
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The expression of the Chlamydomonas reinhardtii cytochrome c gene was studied at the steady-state mRNA level. The inclusion of acetate under illumination produced a marked increase in cytochrome c transcripts. This effect was not affected by two inhibitors of mitochondrial energy metabolism. Three different obligate photoautotrophic mutants with defective mitochondria showed normal levels of induction, suggesting that utilization of acetate for respiration is not required for this process. Light, in the presence or absence of acetate, also promoted an increase in cytochrome c transcript levels. This effect could be abolished by treatment of the cells with an inhibitor of the photosynthetic electron transport chain, suggesting that light acts through photosynthesis to promote the induction. In addition, a genomic clone encompassing the Chlamydomonas cytochrome c gene has been isolated and analyzed. The gene contains three introns, two of which are located at positions similar to those in the rice and Arabidopsis cytochrome c genes, indicating the existence of an evolutionary link. It is concluded that the cytochrome c gene from C. reinhardtii is subject to metabolic regulation through a mechanism that responds to the intracellular level of either acetate or a compound derived from its metabolization through a pathway different from mitochondrial respiration.
Fil: Felitti, Silvina Andrea. Universidad Nacional del Litoral; Argentina
Fil: Chan, Raquel Lia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Sierra, Manuel González. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Química Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Química Rosario; Argentina
Fil: Gonzalez, Daniel Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Materia
CHLAMYDOMONAS REINHARDTII
CYTOCHROME C
EXON/INTRON STRUCTURE
GENE EXPRESSION
MITOCHONDRION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/85660
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/85660
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The cytochrome c gene from the green alga Chlamydomonas reinhardtii. Structure and expression in wild-type cells and in obligate photoautotrophic (dk) mutantsFelitti, Silvina AndreaChan, Raquel LiaSierra, Manuel GonzálezGonzalez, Daniel HectorCHLAMYDOMONAS REINHARDTIICYTOCHROME CEXON/INTRON STRUCTUREGENE EXPRESSIONMITOCHONDRIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The expression of the Chlamydomonas reinhardtii cytochrome c gene was studied at the steady-state mRNA level. The inclusion of acetate under illumination produced a marked increase in cytochrome c transcripts. This effect was not affected by two inhibitors of mitochondrial energy metabolism. Three different obligate photoautotrophic mutants with defective mitochondria showed normal levels of induction, suggesting that utilization of acetate for respiration is not required for this process. Light, in the presence or absence of acetate, also promoted an increase in cytochrome c transcript levels. This effect could be abolished by treatment of the cells with an inhibitor of the photosynthetic electron transport chain, suggesting that light acts through photosynthesis to promote the induction. In addition, a genomic clone encompassing the Chlamydomonas cytochrome c gene has been isolated and analyzed. The gene contains three introns, two of which are located at positions similar to those in the rice and Arabidopsis cytochrome c genes, indicating the existence of an evolutionary link. It is concluded that the cytochrome c gene from C. reinhardtii is subject to metabolic regulation through a mechanism that responds to the intracellular level of either acetate or a compound derived from its metabolization through a pathway different from mitochondrial respiration.Fil: Felitti, Silvina Andrea. Universidad Nacional del Litoral; ArgentinaFil: Chan, Raquel Lia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Sierra, Manuel González. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Química Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Química Rosario; ArgentinaFil: Gonzalez, Daniel Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaOxford University Press2000-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/85660Felitti, Silvina Andrea; Chan, Raquel Lia; Sierra, Manuel González; Gonzalez, Daniel Hector; The cytochrome c gene from the green alga Chlamydomonas reinhardtii. Structure and expression in wild-type cells and in obligate photoautotrophic (dk) mutants; Oxford University Press; Plant And Cell Physiology; 41; 10; 12-2000; 1149-11560032-0781CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1093/pcp/pcd044info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:33:50Zoai:ri.conicet.gov.ar:11336/85660instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:33:50.957CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The cytochrome c gene from the green alga Chlamydomonas reinhardtii. Structure and expression in wild-type cells and in obligate photoautotrophic (dk) mutants
title The cytochrome c gene from the green alga Chlamydomonas reinhardtii. Structure and expression in wild-type cells and in obligate photoautotrophic (dk) mutants
spellingShingle The cytochrome c gene from the green alga Chlamydomonas reinhardtii. Structure and expression in wild-type cells and in obligate photoautotrophic (dk) mutants
Felitti, Silvina Andrea
CHLAMYDOMONAS REINHARDTII
CYTOCHROME C
EXON/INTRON STRUCTURE
GENE EXPRESSION
MITOCHONDRION
title_short The cytochrome c gene from the green alga Chlamydomonas reinhardtii. Structure and expression in wild-type cells and in obligate photoautotrophic (dk) mutants
title_full The cytochrome c gene from the green alga Chlamydomonas reinhardtii. Structure and expression in wild-type cells and in obligate photoautotrophic (dk) mutants
title_fullStr The cytochrome c gene from the green alga Chlamydomonas reinhardtii. Structure and expression in wild-type cells and in obligate photoautotrophic (dk) mutants
title_full_unstemmed The cytochrome c gene from the green alga Chlamydomonas reinhardtii. Structure and expression in wild-type cells and in obligate photoautotrophic (dk) mutants
title_sort The cytochrome c gene from the green alga Chlamydomonas reinhardtii. Structure and expression in wild-type cells and in obligate photoautotrophic (dk) mutants
dc.creator.none.fl_str_mv Felitti, Silvina Andrea
Chan, Raquel Lia
Sierra, Manuel González
Gonzalez, Daniel Hector
author Felitti, Silvina Andrea
author_facet Felitti, Silvina Andrea
Chan, Raquel Lia
Sierra, Manuel González
Gonzalez, Daniel Hector
author_role author
author2 Chan, Raquel Lia
Sierra, Manuel González
Gonzalez, Daniel Hector
author2_role author
author
author
dc.subject.none.fl_str_mv CHLAMYDOMONAS REINHARDTII
CYTOCHROME C
EXON/INTRON STRUCTURE
GENE EXPRESSION
MITOCHONDRION
topic CHLAMYDOMONAS REINHARDTII
CYTOCHROME C
EXON/INTRON STRUCTURE
GENE EXPRESSION
MITOCHONDRION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The expression of the Chlamydomonas reinhardtii cytochrome c gene was studied at the steady-state mRNA level. The inclusion of acetate under illumination produced a marked increase in cytochrome c transcripts. This effect was not affected by two inhibitors of mitochondrial energy metabolism. Three different obligate photoautotrophic mutants with defective mitochondria showed normal levels of induction, suggesting that utilization of acetate for respiration is not required for this process. Light, in the presence or absence of acetate, also promoted an increase in cytochrome c transcript levels. This effect could be abolished by treatment of the cells with an inhibitor of the photosynthetic electron transport chain, suggesting that light acts through photosynthesis to promote the induction. In addition, a genomic clone encompassing the Chlamydomonas cytochrome c gene has been isolated and analyzed. The gene contains three introns, two of which are located at positions similar to those in the rice and Arabidopsis cytochrome c genes, indicating the existence of an evolutionary link. It is concluded that the cytochrome c gene from C. reinhardtii is subject to metabolic regulation through a mechanism that responds to the intracellular level of either acetate or a compound derived from its metabolization through a pathway different from mitochondrial respiration.
Fil: Felitti, Silvina Andrea. Universidad Nacional del Litoral; Argentina
Fil: Chan, Raquel Lia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Sierra, Manuel González. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Química Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Química Rosario; Argentina
Fil: Gonzalez, Daniel Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
description The expression of the Chlamydomonas reinhardtii cytochrome c gene was studied at the steady-state mRNA level. The inclusion of acetate under illumination produced a marked increase in cytochrome c transcripts. This effect was not affected by two inhibitors of mitochondrial energy metabolism. Three different obligate photoautotrophic mutants with defective mitochondria showed normal levels of induction, suggesting that utilization of acetate for respiration is not required for this process. Light, in the presence or absence of acetate, also promoted an increase in cytochrome c transcript levels. This effect could be abolished by treatment of the cells with an inhibitor of the photosynthetic electron transport chain, suggesting that light acts through photosynthesis to promote the induction. In addition, a genomic clone encompassing the Chlamydomonas cytochrome c gene has been isolated and analyzed. The gene contains three introns, two of which are located at positions similar to those in the rice and Arabidopsis cytochrome c genes, indicating the existence of an evolutionary link. It is concluded that the cytochrome c gene from C. reinhardtii is subject to metabolic regulation through a mechanism that responds to the intracellular level of either acetate or a compound derived from its metabolization through a pathway different from mitochondrial respiration.
publishDate 2000
dc.date.none.fl_str_mv 2000-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/85660
Felitti, Silvina Andrea; Chan, Raquel Lia; Sierra, Manuel González; Gonzalez, Daniel Hector; The cytochrome c gene from the green alga Chlamydomonas reinhardtii. Structure and expression in wild-type cells and in obligate photoautotrophic (dk) mutants; Oxford University Press; Plant And Cell Physiology; 41; 10; 12-2000; 1149-1156
0032-0781
CONICET Digital
CONICET
url http://hdl.handle.net/11336/85660
identifier_str_mv Felitti, Silvina Andrea; Chan, Raquel Lia; Sierra, Manuel González; Gonzalez, Daniel Hector; The cytochrome c gene from the green alga Chlamydomonas reinhardtii. Structure and expression in wild-type cells and in obligate photoautotrophic (dk) mutants; Oxford University Press; Plant And Cell Physiology; 41; 10; 12-2000; 1149-1156
0032-0781
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1093/pcp/pcd044
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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