Isocitrate lyase activity and antioxidant responses in copper-stressed cultures of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae)

Autores
Sztrum, Abelardo; Sabatini, Sebastian Eduardo; Rodríguez, María Cecilia
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The effect of copper-imposed oxidative stress on isocitrate lyase (ICL) activity was studied in a Chlamydomonas reinhardtii wild type and a copper-tolerant pleiotropic mutant strain. Suspension batch bioassays were performed adding copper chloride under moderate irradiation. Two harvest points were selected to represent the acute and restoration phases and oxidative stress indicators were measured: antioxidative metabolites, phenolic compounds, ascorbic acid, abundance of thiobarbituric reactive substances, and activities of detoxifying enzymes such as catalase and ascorbate peroxidase. Copper stress under moderate irradiance favours acetate assimilation through enhanced ICL activity, especially in the acute phase. Though the mutant strain exhibited at least twofold ICL activity in the acute phase compared to the wild type, there was no significant difference in copper tolerance between strains. The higher ICL activity under copper stress may be an adaptation to promote mixotrophic organic carbon assimilation when photosynthetic carbon dioxide fixation is impaired. Thus, the higher ICL activity has the potential to be a useful indicator in acetate-flagellate bioassays.
Fil: Sztrum, Abelardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación Pro Salud y Medio Ambiente. Instituto de Ciencias Ambientales y Salud; Argentina
Fil: Sabatini, Sebastian Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Rodríguez, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Materia
Ascorbate Peroxidase
Catalase
Chlamydomonas Reinhardtii
Copper
Isocitrate Lyase
Lipid Peroxidation
Oxidative Stress
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/60894
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/60894
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Isocitrate lyase activity and antioxidant responses in copper-stressed cultures of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae)Sztrum, AbelardoSabatini, Sebastian EduardoRodríguez, María CeciliaAscorbate PeroxidaseCatalaseChlamydomonas ReinhardtiiCopperIsocitrate LyaseLipid PeroxidationOxidative Stresshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The effect of copper-imposed oxidative stress on isocitrate lyase (ICL) activity was studied in a Chlamydomonas reinhardtii wild type and a copper-tolerant pleiotropic mutant strain. Suspension batch bioassays were performed adding copper chloride under moderate irradiation. Two harvest points were selected to represent the acute and restoration phases and oxidative stress indicators were measured: antioxidative metabolites, phenolic compounds, ascorbic acid, abundance of thiobarbituric reactive substances, and activities of detoxifying enzymes such as catalase and ascorbate peroxidase. Copper stress under moderate irradiance favours acetate assimilation through enhanced ICL activity, especially in the acute phase. Though the mutant strain exhibited at least twofold ICL activity in the acute phase compared to the wild type, there was no significant difference in copper tolerance between strains. The higher ICL activity under copper stress may be an adaptation to promote mixotrophic organic carbon assimilation when photosynthetic carbon dioxide fixation is impaired. Thus, the higher ICL activity has the potential to be a useful indicator in acetate-flagellate bioassays.Fil: Sztrum, Abelardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación Pro Salud y Medio Ambiente. Instituto de Ciencias Ambientales y Salud; ArgentinaFil: Sabatini, Sebastian Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaFil: Rodríguez, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; ArgentinaInternational Phycological Society2012-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/60894Sztrum, Abelardo; Sabatini, Sebastian Eduardo; Rodríguez, María Cecilia; Isocitrate lyase activity and antioxidant responses in copper-stressed cultures of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae); International Phycological Society; Phycologia; 51; 2; 3-2012; 135-1430031-8884CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.2216/10-56.1info:eu-repo/semantics/altIdentifier/url/http://www.phycologia.org/doi/abs/10.2216/10-56.1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:21:21Zoai:ri.conicet.gov.ar:11336/60894instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:21:21.659CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Isocitrate lyase activity and antioxidant responses in copper-stressed cultures of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae)
title Isocitrate lyase activity and antioxidant responses in copper-stressed cultures of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae)
spellingShingle Isocitrate lyase activity and antioxidant responses in copper-stressed cultures of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae)
Sztrum, Abelardo
Ascorbate Peroxidase
Catalase
Chlamydomonas Reinhardtii
Copper
Isocitrate Lyase
Lipid Peroxidation
Oxidative Stress
title_short Isocitrate lyase activity and antioxidant responses in copper-stressed cultures of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae)
title_full Isocitrate lyase activity and antioxidant responses in copper-stressed cultures of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae)
title_fullStr Isocitrate lyase activity and antioxidant responses in copper-stressed cultures of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae)
title_full_unstemmed Isocitrate lyase activity and antioxidant responses in copper-stressed cultures of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae)
title_sort Isocitrate lyase activity and antioxidant responses in copper-stressed cultures of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae)
dc.creator.none.fl_str_mv Sztrum, Abelardo
Sabatini, Sebastian Eduardo
Rodríguez, María Cecilia
author Sztrum, Abelardo
author_facet Sztrum, Abelardo
Sabatini, Sebastian Eduardo
Rodríguez, María Cecilia
author_role author
author2 Sabatini, Sebastian Eduardo
Rodríguez, María Cecilia
author2_role author
author
dc.subject.none.fl_str_mv Ascorbate Peroxidase
Catalase
Chlamydomonas Reinhardtii
Copper
Isocitrate Lyase
Lipid Peroxidation
Oxidative Stress
topic Ascorbate Peroxidase
Catalase
Chlamydomonas Reinhardtii
Copper
Isocitrate Lyase
Lipid Peroxidation
Oxidative Stress
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The effect of copper-imposed oxidative stress on isocitrate lyase (ICL) activity was studied in a Chlamydomonas reinhardtii wild type and a copper-tolerant pleiotropic mutant strain. Suspension batch bioassays were performed adding copper chloride under moderate irradiation. Two harvest points were selected to represent the acute and restoration phases and oxidative stress indicators were measured: antioxidative metabolites, phenolic compounds, ascorbic acid, abundance of thiobarbituric reactive substances, and activities of detoxifying enzymes such as catalase and ascorbate peroxidase. Copper stress under moderate irradiance favours acetate assimilation through enhanced ICL activity, especially in the acute phase. Though the mutant strain exhibited at least twofold ICL activity in the acute phase compared to the wild type, there was no significant difference in copper tolerance between strains. The higher ICL activity under copper stress may be an adaptation to promote mixotrophic organic carbon assimilation when photosynthetic carbon dioxide fixation is impaired. Thus, the higher ICL activity has the potential to be a useful indicator in acetate-flagellate bioassays.
Fil: Sztrum, Abelardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación Pro Salud y Medio Ambiente. Instituto de Ciencias Ambientales y Salud; Argentina
Fil: Sabatini, Sebastian Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Rodríguez, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
description The effect of copper-imposed oxidative stress on isocitrate lyase (ICL) activity was studied in a Chlamydomonas reinhardtii wild type and a copper-tolerant pleiotropic mutant strain. Suspension batch bioassays were performed adding copper chloride under moderate irradiation. Two harvest points were selected to represent the acute and restoration phases and oxidative stress indicators were measured: antioxidative metabolites, phenolic compounds, ascorbic acid, abundance of thiobarbituric reactive substances, and activities of detoxifying enzymes such as catalase and ascorbate peroxidase. Copper stress under moderate irradiance favours acetate assimilation through enhanced ICL activity, especially in the acute phase. Though the mutant strain exhibited at least twofold ICL activity in the acute phase compared to the wild type, there was no significant difference in copper tolerance between strains. The higher ICL activity under copper stress may be an adaptation to promote mixotrophic organic carbon assimilation when photosynthetic carbon dioxide fixation is impaired. Thus, the higher ICL activity has the potential to be a useful indicator in acetate-flagellate bioassays.
publishDate 2012
dc.date.none.fl_str_mv 2012-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/60894
Sztrum, Abelardo; Sabatini, Sebastian Eduardo; Rodríguez, María Cecilia; Isocitrate lyase activity and antioxidant responses in copper-stressed cultures of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae); International Phycological Society; Phycologia; 51; 2; 3-2012; 135-143
0031-8884
CONICET Digital
CONICET
url http://hdl.handle.net/11336/60894
identifier_str_mv Sztrum, Abelardo; Sabatini, Sebastian Eduardo; Rodríguez, María Cecilia; Isocitrate lyase activity and antioxidant responses in copper-stressed cultures of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae); International Phycological Society; Phycologia; 51; 2; 3-2012; 135-143
0031-8884
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.2216/10-56.1
info:eu-repo/semantics/altIdentifier/url/http://www.phycologia.org/doi/abs/10.2216/10-56.1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv International Phycological Society
publisher.none.fl_str_mv International Phycological Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.48226

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