Impact of NaCl on the macromolecular composition of unsaturated Pseudomonas capeferrum WCS358 biofilms

Autores
Lacosegliaz, Mariano José; Torres, Mariela Analía; de Cristobal, Ricardo Ezequiel; Nieto Peñalver, Carlos Gabriel
Año de publicación
2022
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
High NaCl concentrations in soils affect negatively the production, nutritional, and economic value of crops, as well as its biodiversity. In response to this problem, a large number of studies on plant growth-promoting rhizobacteria (PGPR) have been developed, as they may improve the uptake of water and nutrients and alleviate different types of stresses in plants. These bacteria colonize the rhizosphere forming biofilms that are exposed to constant fluctuations in water availability. For this reason, in this work, we utilize an unsaturated biofilm model as an approximation to the conditions to which rhizobacteria are exposed. Pseudomonas (P.) capeferrum WCS358 produces pyoverdine and induces a systemic response in plants. Its Quorum Sensing (QS) system regulates physiological processes relevant to the colonization of the host and survival. The aim of this work was to analyze the impact of salt stress on growth and the characteristics of the unsaturated biofilm of P. capeferrum WCS358, and the influence of QS regulation. For this, we employed polycarbonate membranes and an artificial root exudates culture medium supplemented with 0.2 M NaCl as saline stress. Membranes were inoculated with bacterial suspensions (0.1 OD600) and incubated at 30 °C for 72 hours. Unsaturated biofilms were broken up by vigorously vortexing the membrane in 1 mL physiological solution. CFU mL-1 was determined after plating serial dilutions. Carbohydrates were quantified using the phenol?sulfuric method with glucose as a standard, extracellular proteins were quantified using the Bradford method. Acyl homoserine lactones (AHLs) production was determined after solvent extraction, separation by thin-layer chromatography, and subsequent detection with bioassays developed with Agrobacterium (A.) tumefaciens NT1 (pZLR4). Results showed that neither saline stress nor QS activity decreased biofilm growth of P. capeferrum WCS358. While the attenuation of the QS activity augmented 13% of the exopolysaccharides in the unsaturated biofilms, NaCl decreased by 28% of these values. Proteins in the matrix were not affected by QS. However, saline stress increased by 30% the protein concentration. RP-TLC developed with A. tumefaciens NT1 (pZLR4) showed smaller and weaker spots of AHLs. We conclude that saline stress and the QS system modify the characteristics of the unsaturated biofilms of P. capeferrum WCS358. At the same time, NaCl modulates the QS activity of this microorganism. These results are relevant for a PGPR, considering the large surfaces of saline soils in our country.
Fil: Lacosegliaz, Mariano José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Torres, Mariela Analía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: de Cristobal, Ricardo Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química Biológica; Argentina
Fil: Nieto Peñalver, Carlos Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina
XVII Congreso Argentino de Microbiología General
Los Cocos
Argentina
Sociedad Argentina de Microbiología General
Materia
NaCl
UNSATURATED BIOFILM
QUORUM SENSING
PSEUDOMONAS CAPEFERRUM WCS358
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/210164
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/210164
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Impact of NaCl on the macromolecular composition of unsaturated Pseudomonas capeferrum WCS358 biofilmsLacosegliaz, Mariano JoséTorres, Mariela Analíade Cristobal, Ricardo EzequielNieto Peñalver, Carlos GabrielNaClUNSATURATED BIOFILMQUORUM SENSINGPSEUDOMONAS CAPEFERRUM WCS358https://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2High NaCl concentrations in soils affect negatively the production, nutritional, and economic value of crops, as well as its biodiversity. In response to this problem, a large number of studies on plant growth-promoting rhizobacteria (PGPR) have been developed, as they may improve the uptake of water and nutrients and alleviate different types of stresses in plants. These bacteria colonize the rhizosphere forming biofilms that are exposed to constant fluctuations in water availability. For this reason, in this work, we utilize an unsaturated biofilm model as an approximation to the conditions to which rhizobacteria are exposed. Pseudomonas (P.) capeferrum WCS358 produces pyoverdine and induces a systemic response in plants. Its Quorum Sensing (QS) system regulates physiological processes relevant to the colonization of the host and survival. The aim of this work was to analyze the impact of salt stress on growth and the characteristics of the unsaturated biofilm of P. capeferrum WCS358, and the influence of QS regulation. For this, we employed polycarbonate membranes and an artificial root exudates culture medium supplemented with 0.2 M NaCl as saline stress. Membranes were inoculated with bacterial suspensions (0.1 OD600) and incubated at 30 °C for 72 hours. Unsaturated biofilms were broken up by vigorously vortexing the membrane in 1 mL physiological solution. CFU mL-1 was determined after plating serial dilutions. Carbohydrates were quantified using the phenol?sulfuric method with glucose as a standard, extracellular proteins were quantified using the Bradford method. Acyl homoserine lactones (AHLs) production was determined after solvent extraction, separation by thin-layer chromatography, and subsequent detection with bioassays developed with Agrobacterium (A.) tumefaciens NT1 (pZLR4). Results showed that neither saline stress nor QS activity decreased biofilm growth of P. capeferrum WCS358. While the attenuation of the QS activity augmented 13% of the exopolysaccharides in the unsaturated biofilms, NaCl decreased by 28% of these values. Proteins in the matrix were not affected by QS. However, saline stress increased by 30% the protein concentration. RP-TLC developed with A. tumefaciens NT1 (pZLR4) showed smaller and weaker spots of AHLs. We conclude that saline stress and the QS system modify the characteristics of the unsaturated biofilms of P. capeferrum WCS358. At the same time, NaCl modulates the QS activity of this microorganism. These results are relevant for a PGPR, considering the large surfaces of saline soils in our country.Fil: Lacosegliaz, Mariano José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Torres, Mariela Analía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: de Cristobal, Ricardo Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química Biológica; ArgentinaFil: Nieto Peñalver, Carlos Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; ArgentinaXVII Congreso Argentino de Microbiología GeneralLos CocosArgentinaSociedad Argentina de Microbiología GeneralSociedad Argentina de Microbiología General2022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/210164Impact of NaCl on the macromolecular composition of unsaturated Pseudomonas capeferrum WCS358 biofilms; XVII Congreso Argentino de Microbiología General; Los Cocos; Argentina; 2022; 154-154CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://samige.org.ar/wp-content/uploads/2022/10/Libro-de-Resumenes-SAMIGE-2022_final.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:50:39Zoai:ri.conicet.gov.ar:11336/210164instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:50:39.683CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Impact of NaCl on the macromolecular composition of unsaturated Pseudomonas capeferrum WCS358 biofilms
title Impact of NaCl on the macromolecular composition of unsaturated Pseudomonas capeferrum WCS358 biofilms
spellingShingle Impact of NaCl on the macromolecular composition of unsaturated Pseudomonas capeferrum WCS358 biofilms
Lacosegliaz, Mariano José
NaCl
UNSATURATED BIOFILM
QUORUM SENSING
PSEUDOMONAS CAPEFERRUM WCS358
title_short Impact of NaCl on the macromolecular composition of unsaturated Pseudomonas capeferrum WCS358 biofilms
title_full Impact of NaCl on the macromolecular composition of unsaturated Pseudomonas capeferrum WCS358 biofilms
title_fullStr Impact of NaCl on the macromolecular composition of unsaturated Pseudomonas capeferrum WCS358 biofilms
title_full_unstemmed Impact of NaCl on the macromolecular composition of unsaturated Pseudomonas capeferrum WCS358 biofilms
title_sort Impact of NaCl on the macromolecular composition of unsaturated Pseudomonas capeferrum WCS358 biofilms
dc.creator.none.fl_str_mv Lacosegliaz, Mariano José
Torres, Mariela Analía
de Cristobal, Ricardo Ezequiel
Nieto Peñalver, Carlos Gabriel
author Lacosegliaz, Mariano José
author_facet Lacosegliaz, Mariano José
Torres, Mariela Analía
de Cristobal, Ricardo Ezequiel
Nieto Peñalver, Carlos Gabriel
author_role author
author2 Torres, Mariela Analía
de Cristobal, Ricardo Ezequiel
Nieto Peñalver, Carlos Gabriel
author2_role author
author
author
dc.subject.none.fl_str_mv NaCl
UNSATURATED BIOFILM
QUORUM SENSING
PSEUDOMONAS CAPEFERRUM WCS358
topic NaCl
UNSATURATED BIOFILM
QUORUM SENSING
PSEUDOMONAS CAPEFERRUM WCS358
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv High NaCl concentrations in soils affect negatively the production, nutritional, and economic value of crops, as well as its biodiversity. In response to this problem, a large number of studies on plant growth-promoting rhizobacteria (PGPR) have been developed, as they may improve the uptake of water and nutrients and alleviate different types of stresses in plants. These bacteria colonize the rhizosphere forming biofilms that are exposed to constant fluctuations in water availability. For this reason, in this work, we utilize an unsaturated biofilm model as an approximation to the conditions to which rhizobacteria are exposed. Pseudomonas (P.) capeferrum WCS358 produces pyoverdine and induces a systemic response in plants. Its Quorum Sensing (QS) system regulates physiological processes relevant to the colonization of the host and survival. The aim of this work was to analyze the impact of salt stress on growth and the characteristics of the unsaturated biofilm of P. capeferrum WCS358, and the influence of QS regulation. For this, we employed polycarbonate membranes and an artificial root exudates culture medium supplemented with 0.2 M NaCl as saline stress. Membranes were inoculated with bacterial suspensions (0.1 OD600) and incubated at 30 °C for 72 hours. Unsaturated biofilms were broken up by vigorously vortexing the membrane in 1 mL physiological solution. CFU mL-1 was determined after plating serial dilutions. Carbohydrates were quantified using the phenol?sulfuric method with glucose as a standard, extracellular proteins were quantified using the Bradford method. Acyl homoserine lactones (AHLs) production was determined after solvent extraction, separation by thin-layer chromatography, and subsequent detection with bioassays developed with Agrobacterium (A.) tumefaciens NT1 (pZLR4). Results showed that neither saline stress nor QS activity decreased biofilm growth of P. capeferrum WCS358. While the attenuation of the QS activity augmented 13% of the exopolysaccharides in the unsaturated biofilms, NaCl decreased by 28% of these values. Proteins in the matrix were not affected by QS. However, saline stress increased by 30% the protein concentration. RP-TLC developed with A. tumefaciens NT1 (pZLR4) showed smaller and weaker spots of AHLs. We conclude that saline stress and the QS system modify the characteristics of the unsaturated biofilms of P. capeferrum WCS358. At the same time, NaCl modulates the QS activity of this microorganism. These results are relevant for a PGPR, considering the large surfaces of saline soils in our country.
Fil: Lacosegliaz, Mariano José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Torres, Mariela Analía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: de Cristobal, Ricardo Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química Biológica; Argentina
Fil: Nieto Peñalver, Carlos Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina
XVII Congreso Argentino de Microbiología General
Los Cocos
Argentina
Sociedad Argentina de Microbiología General
description High NaCl concentrations in soils affect negatively the production, nutritional, and economic value of crops, as well as its biodiversity. In response to this problem, a large number of studies on plant growth-promoting rhizobacteria (PGPR) have been developed, as they may improve the uptake of water and nutrients and alleviate different types of stresses in plants. These bacteria colonize the rhizosphere forming biofilms that are exposed to constant fluctuations in water availability. For this reason, in this work, we utilize an unsaturated biofilm model as an approximation to the conditions to which rhizobacteria are exposed. Pseudomonas (P.) capeferrum WCS358 produces pyoverdine and induces a systemic response in plants. Its Quorum Sensing (QS) system regulates physiological processes relevant to the colonization of the host and survival. The aim of this work was to analyze the impact of salt stress on growth and the characteristics of the unsaturated biofilm of P. capeferrum WCS358, and the influence of QS regulation. For this, we employed polycarbonate membranes and an artificial root exudates culture medium supplemented with 0.2 M NaCl as saline stress. Membranes were inoculated with bacterial suspensions (0.1 OD600) and incubated at 30 °C for 72 hours. Unsaturated biofilms were broken up by vigorously vortexing the membrane in 1 mL physiological solution. CFU mL-1 was determined after plating serial dilutions. Carbohydrates were quantified using the phenol?sulfuric method with glucose as a standard, extracellular proteins were quantified using the Bradford method. Acyl homoserine lactones (AHLs) production was determined after solvent extraction, separation by thin-layer chromatography, and subsequent detection with bioassays developed with Agrobacterium (A.) tumefaciens NT1 (pZLR4). Results showed that neither saline stress nor QS activity decreased biofilm growth of P. capeferrum WCS358. While the attenuation of the QS activity augmented 13% of the exopolysaccharides in the unsaturated biofilms, NaCl decreased by 28% of these values. Proteins in the matrix were not affected by QS. However, saline stress increased by 30% the protein concentration. RP-TLC developed with A. tumefaciens NT1 (pZLR4) showed smaller and weaker spots of AHLs. We conclude that saline stress and the QS system modify the characteristics of the unsaturated biofilms of P. capeferrum WCS358. At the same time, NaCl modulates the QS activity of this microorganism. These results are relevant for a PGPR, considering the large surfaces of saline soils in our country.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/210164
Impact of NaCl on the macromolecular composition of unsaturated Pseudomonas capeferrum WCS358 biofilms; XVII Congreso Argentino de Microbiología General; Los Cocos; Argentina; 2022; 154-154
CONICET Digital
CONICET
url http://hdl.handle.net/11336/210164
identifier_str_mv Impact of NaCl on the macromolecular composition of unsaturated Pseudomonas capeferrum WCS358 biofilms; XVII Congreso Argentino de Microbiología General; Los Cocos; Argentina; 2022; 154-154
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://samige.org.ar/wp-content/uploads/2022/10/Libro-de-Resumenes-SAMIGE-2022_final.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Microbiología General
publisher.none.fl_str_mv Sociedad Argentina de Microbiología General
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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