Preclinical Study of DCD and Normothermic Perfusion for Visceral Transplantation

Autores
Serradilla, Javier; Andrés Moreno, Ane Miren; Talayero, Paloma; Burgos, Paula; Machuca, Mariana Alejandra; Camps Ortega, Onys; Vallejo, María Teresa; Rubio Bolívar, Francisco Javier; Bueno, Alba; Sánchez, Alba; Zambrano, Cristina; De la Torre Ramos, Carlos Andrés; Rodríguez, Olaia; Largo, Carlota; Serrano, Pilar; Prieto Bozano, Gerardo; Ramos, Esther; López Santamaría, Manuel; Stringa, Pablo Luis; Hernández, Francisco
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Considering recent clinical and experimental evidence, expectations for using DCD-derived intestines have increased considerably. However, more knowledge about DCD procedure and long-term results after intestinal transplantation (ITx) is needed. We aimed to describe in detail a DCD procedure for ITx using normothermic regional perfusion (NRP) in a preclinical model. Small bowel was obtained from pigs donors after 1 h of NRP and transplanted to the recipients. Graft Intestinal samples were obtained during the procedure and after transplantation. Ischemia-reperfusion injury (Park-Chiu score), graft rejection and transplanted intestines absorptive function were evaluated. Seven of 8 DCD procedures with NRP and ITx were successful (87.5%), with a good graft reperfusion and an excellent recovery of the recipient. The architecture of grafts was well conserved during NRP. After an initial damage of Park-chiu score of 4, all grafts recovered from ischemia-reperfusion, with no or very subtle alterations 2 days after ITx. Most recipients (71.5%) did not show signs of rejection. Only two cases demonstrated histologic signs of mild rejection 7 days after ITx. Interestingly intestinal grafts showed good absorptive capacity. The study´s results support the viability of intestinal grafts from DCD using NRP, contributing more evidence for the use of DCD for ITx.
Fil: Serradilla, Javier. Hospital Universitario la Paz; España
Fil: Andrés Moreno, Ane Miren. Hospital Universitario la Paz; España
Fil: Talayero, Paloma. No especifíca;
Fil: Burgos, Paula. Hospital Universitario la Paz; España
Fil: Machuca, Mariana Alejandra. Universidad Nacional de La Plata; Argentina
Fil: Camps Ortega, Onys. Instituto de Investigación Hospital Universitario la Paz; España
Fil: Vallejo, María Teresa. Instituto de Investigación Hospital Universitario la Paz; España
Fil: Rubio Bolívar, Francisco Javier. Instituto de Investigación Hospital Universitario la Paz; España
Fil: Bueno, Alba. Hospital Universitario la Paz; España
Fil: Sánchez, Alba. Hospital Universitario la Paz; España
Fil: Zambrano, Cristina. Hospital Universitario la Paz; España
Fil: De la Torre Ramos, Carlos Andrés. Hospital Universitario la Paz; España
Fil: Rodríguez, Olaia. Instituto de Investigación Hospital Universitario la Paz; España
Fil: Largo, Carlota. Instituto de Investigación Hospital Universitario la Paz; España
Fil: Serrano, Pilar. Hospital Universitario la Paz; España
Fil: Prieto Bozano, Gerardo. Hospital Universitario la Paz; España
Fil: Ramos, Esther. Hospital Universitario la Paz; España
Fil: López Santamaría, Manuel. Hospital Universitario la Paz; España
Fil: Stringa, Pablo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; Argentina
Fil: Hernández, Francisco. Hospital Universitario la Paz; España
Materia
INTESTINAL TRANSPLANTATION
DCD
SOLID ORGAN TRANSPLANTATION
ORGAN PROCUREMENT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/250368
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/250368
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Preclinical Study of DCD and Normothermic Perfusion for Visceral TransplantationSerradilla, JavierAndrés Moreno, Ane MirenTalayero, PalomaBurgos, PaulaMachuca, Mariana AlejandraCamps Ortega, OnysVallejo, María TeresaRubio Bolívar, Francisco JavierBueno, AlbaSánchez, AlbaZambrano, CristinaDe la Torre Ramos, Carlos AndrésRodríguez, OlaiaLargo, CarlotaSerrano, PilarPrieto Bozano, GerardoRamos, EstherLópez Santamaría, ManuelStringa, Pablo LuisHernández, FranciscoINTESTINAL TRANSPLANTATIONDCDSOLID ORGAN TRANSPLANTATIONORGAN PROCUREMENThttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Considering recent clinical and experimental evidence, expectations for using DCD-derived intestines have increased considerably. However, more knowledge about DCD procedure and long-term results after intestinal transplantation (ITx) is needed. We aimed to describe in detail a DCD procedure for ITx using normothermic regional perfusion (NRP) in a preclinical model. Small bowel was obtained from pigs donors after 1 h of NRP and transplanted to the recipients. Graft Intestinal samples were obtained during the procedure and after transplantation. Ischemia-reperfusion injury (Park-Chiu score), graft rejection and transplanted intestines absorptive function were evaluated. Seven of 8 DCD procedures with NRP and ITx were successful (87.5%), with a good graft reperfusion and an excellent recovery of the recipient. The architecture of grafts was well conserved during NRP. After an initial damage of Park-chiu score of 4, all grafts recovered from ischemia-reperfusion, with no or very subtle alterations 2 days after ITx. Most recipients (71.5%) did not show signs of rejection. Only two cases demonstrated histologic signs of mild rejection 7 days after ITx. Interestingly intestinal grafts showed good absorptive capacity. The study´s results support the viability of intestinal grafts from DCD using NRP, contributing more evidence for the use of DCD for ITx.Fil: Serradilla, Javier. Hospital Universitario la Paz; EspañaFil: Andrés Moreno, Ane Miren. Hospital Universitario la Paz; EspañaFil: Talayero, Paloma. No especifíca;Fil: Burgos, Paula. Hospital Universitario la Paz; EspañaFil: Machuca, Mariana Alejandra. Universidad Nacional de La Plata; ArgentinaFil: Camps Ortega, Onys. Instituto de Investigación Hospital Universitario la Paz; EspañaFil: Vallejo, María Teresa. Instituto de Investigación Hospital Universitario la Paz; EspañaFil: Rubio Bolívar, Francisco Javier. Instituto de Investigación Hospital Universitario la Paz; EspañaFil: Bueno, Alba. Hospital Universitario la Paz; EspañaFil: Sánchez, Alba. Hospital Universitario la Paz; EspañaFil: Zambrano, Cristina. Hospital Universitario la Paz; EspañaFil: De la Torre Ramos, Carlos Andrés. Hospital Universitario la Paz; EspañaFil: Rodríguez, Olaia. Instituto de Investigación Hospital Universitario la Paz; EspañaFil: Largo, Carlota. Instituto de Investigación Hospital Universitario la Paz; EspañaFil: Serrano, Pilar. Hospital Universitario la Paz; EspañaFil: Prieto Bozano, Gerardo. Hospital Universitario la Paz; EspañaFil: Ramos, Esther. Hospital Universitario la Paz; EspañaFil: López Santamaría, Manuel. Hospital Universitario la Paz; EspañaFil: Stringa, Pablo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; ArgentinaFil: Hernández, Francisco. Hospital Universitario la Paz; EspañaFrontiers Media2023-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/250368Serradilla, Javier; Andrés Moreno, Ane Miren; Talayero, Paloma; Burgos, Paula; Machuca, Mariana Alejandra; et al.; Preclinical Study of DCD and Normothermic Perfusion for Visceral Transplantation; Frontiers Media; Transplant International; 36; 9-2023; 1-101432-2277CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontierspartnerships.org/articles/10.3389/ti.2023.11518/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/ti.2023.11518info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:47:16Zoai:ri.conicet.gov.ar:11336/250368instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:47:16.819CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Preclinical Study of DCD and Normothermic Perfusion for Visceral Transplantation
title Preclinical Study of DCD and Normothermic Perfusion for Visceral Transplantation
spellingShingle Preclinical Study of DCD and Normothermic Perfusion for Visceral Transplantation
Serradilla, Javier
INTESTINAL TRANSPLANTATION
DCD
SOLID ORGAN TRANSPLANTATION
ORGAN PROCUREMENT
title_short Preclinical Study of DCD and Normothermic Perfusion for Visceral Transplantation
title_full Preclinical Study of DCD and Normothermic Perfusion for Visceral Transplantation
title_fullStr Preclinical Study of DCD and Normothermic Perfusion for Visceral Transplantation
title_full_unstemmed Preclinical Study of DCD and Normothermic Perfusion for Visceral Transplantation
title_sort Preclinical Study of DCD and Normothermic Perfusion for Visceral Transplantation
dc.creator.none.fl_str_mv Serradilla, Javier
Andrés Moreno, Ane Miren
Talayero, Paloma
Burgos, Paula
Machuca, Mariana Alejandra
Camps Ortega, Onys
Vallejo, María Teresa
Rubio Bolívar, Francisco Javier
Bueno, Alba
Sánchez, Alba
Zambrano, Cristina
De la Torre Ramos, Carlos Andrés
Rodríguez, Olaia
Largo, Carlota
Serrano, Pilar
Prieto Bozano, Gerardo
Ramos, Esther
López Santamaría, Manuel
Stringa, Pablo Luis
Hernández, Francisco
author Serradilla, Javier
author_facet Serradilla, Javier
Andrés Moreno, Ane Miren
Talayero, Paloma
Burgos, Paula
Machuca, Mariana Alejandra
Camps Ortega, Onys
Vallejo, María Teresa
Rubio Bolívar, Francisco Javier
Bueno, Alba
Sánchez, Alba
Zambrano, Cristina
De la Torre Ramos, Carlos Andrés
Rodríguez, Olaia
Largo, Carlota
Serrano, Pilar
Prieto Bozano, Gerardo
Ramos, Esther
López Santamaría, Manuel
Stringa, Pablo Luis
Hernández, Francisco
author_role author
author2 Andrés Moreno, Ane Miren
Talayero, Paloma
Burgos, Paula
Machuca, Mariana Alejandra
Camps Ortega, Onys
Vallejo, María Teresa
Rubio Bolívar, Francisco Javier
Bueno, Alba
Sánchez, Alba
Zambrano, Cristina
De la Torre Ramos, Carlos Andrés
Rodríguez, Olaia
Largo, Carlota
Serrano, Pilar
Prieto Bozano, Gerardo
Ramos, Esther
López Santamaría, Manuel
Stringa, Pablo Luis
Hernández, Francisco
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv INTESTINAL TRANSPLANTATION
DCD
SOLID ORGAN TRANSPLANTATION
ORGAN PROCUREMENT
topic INTESTINAL TRANSPLANTATION
DCD
SOLID ORGAN TRANSPLANTATION
ORGAN PROCUREMENT
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Considering recent clinical and experimental evidence, expectations for using DCD-derived intestines have increased considerably. However, more knowledge about DCD procedure and long-term results after intestinal transplantation (ITx) is needed. We aimed to describe in detail a DCD procedure for ITx using normothermic regional perfusion (NRP) in a preclinical model. Small bowel was obtained from pigs donors after 1 h of NRP and transplanted to the recipients. Graft Intestinal samples were obtained during the procedure and after transplantation. Ischemia-reperfusion injury (Park-Chiu score), graft rejection and transplanted intestines absorptive function were evaluated. Seven of 8 DCD procedures with NRP and ITx were successful (87.5%), with a good graft reperfusion and an excellent recovery of the recipient. The architecture of grafts was well conserved during NRP. After an initial damage of Park-chiu score of 4, all grafts recovered from ischemia-reperfusion, with no or very subtle alterations 2 days after ITx. Most recipients (71.5%) did not show signs of rejection. Only two cases demonstrated histologic signs of mild rejection 7 days after ITx. Interestingly intestinal grafts showed good absorptive capacity. The study´s results support the viability of intestinal grafts from DCD using NRP, contributing more evidence for the use of DCD for ITx.
Fil: Serradilla, Javier. Hospital Universitario la Paz; España
Fil: Andrés Moreno, Ane Miren. Hospital Universitario la Paz; España
Fil: Talayero, Paloma. No especifíca;
Fil: Burgos, Paula. Hospital Universitario la Paz; España
Fil: Machuca, Mariana Alejandra. Universidad Nacional de La Plata; Argentina
Fil: Camps Ortega, Onys. Instituto de Investigación Hospital Universitario la Paz; España
Fil: Vallejo, María Teresa. Instituto de Investigación Hospital Universitario la Paz; España
Fil: Rubio Bolívar, Francisco Javier. Instituto de Investigación Hospital Universitario la Paz; España
Fil: Bueno, Alba. Hospital Universitario la Paz; España
Fil: Sánchez, Alba. Hospital Universitario la Paz; España
Fil: Zambrano, Cristina. Hospital Universitario la Paz; España
Fil: De la Torre Ramos, Carlos Andrés. Hospital Universitario la Paz; España
Fil: Rodríguez, Olaia. Instituto de Investigación Hospital Universitario la Paz; España
Fil: Largo, Carlota. Instituto de Investigación Hospital Universitario la Paz; España
Fil: Serrano, Pilar. Hospital Universitario la Paz; España
Fil: Prieto Bozano, Gerardo. Hospital Universitario la Paz; España
Fil: Ramos, Esther. Hospital Universitario la Paz; España
Fil: López Santamaría, Manuel. Hospital Universitario la Paz; España
Fil: Stringa, Pablo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; Argentina
Fil: Hernández, Francisco. Hospital Universitario la Paz; España
description Considering recent clinical and experimental evidence, expectations for using DCD-derived intestines have increased considerably. However, more knowledge about DCD procedure and long-term results after intestinal transplantation (ITx) is needed. We aimed to describe in detail a DCD procedure for ITx using normothermic regional perfusion (NRP) in a preclinical model. Small bowel was obtained from pigs donors after 1 h of NRP and transplanted to the recipients. Graft Intestinal samples were obtained during the procedure and after transplantation. Ischemia-reperfusion injury (Park-Chiu score), graft rejection and transplanted intestines absorptive function were evaluated. Seven of 8 DCD procedures with NRP and ITx were successful (87.5%), with a good graft reperfusion and an excellent recovery of the recipient. The architecture of grafts was well conserved during NRP. After an initial damage of Park-chiu score of 4, all grafts recovered from ischemia-reperfusion, with no or very subtle alterations 2 days after ITx. Most recipients (71.5%) did not show signs of rejection. Only two cases demonstrated histologic signs of mild rejection 7 days after ITx. Interestingly intestinal grafts showed good absorptive capacity. The study´s results support the viability of intestinal grafts from DCD using NRP, contributing more evidence for the use of DCD for ITx.
publishDate 2023
dc.date.none.fl_str_mv 2023-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/250368
Serradilla, Javier; Andrés Moreno, Ane Miren; Talayero, Paloma; Burgos, Paula; Machuca, Mariana Alejandra; et al.; Preclinical Study of DCD and Normothermic Perfusion for Visceral Transplantation; Frontiers Media; Transplant International; 36; 9-2023; 1-10
1432-2277
CONICET Digital
CONICET
url http://hdl.handle.net/11336/250368
identifier_str_mv Serradilla, Javier; Andrés Moreno, Ane Miren; Talayero, Paloma; Burgos, Paula; Machuca, Mariana Alejandra; et al.; Preclinical Study of DCD and Normothermic Perfusion for Visceral Transplantation; Frontiers Media; Transplant International; 36; 9-2023; 1-10
1432-2277
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontierspartnerships.org/articles/10.3389/ti.2023.11518/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/ti.2023.11518
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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