Sequential depletion of rat testicular lipids with long-chain and very long-chain polyenoic fatty acids after X-ray-induced interruption of spermatogenesis

Autores
Oresti, Gerardo Martin; Ayuza Aresti, Pablo Leonardo; Gigola, Graciela; Reyes, Luis E.; Aveldaño, Marta Isabel
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
When a single dose of X-rays is applied to the adult rat testis, stem spermatogonia are damaged and spermatogenesis is interrupted. Supported by Sertoli cells, those spermatogenic cells that endure irradiation complete their differentiation and gradually leave the testis as spermatozoa. In this study, the in vivo changes taking place a number of weeks after irradiation revealed cell-specific features of testicular lipid classes. A linear drop, taking about 6 weeks, in testis weight, non-lipid materials, free cholesterol and 22:5n-6-rich glycerophospholipids took place with germ cell depletion. Sphingomyelins and ceramides with non-hydroxy very long chain polyenoic fatty acids (n-VLCPUFA) disappeared in 4 weeks, together with the last spermatocytes, whereas species with 2-hydroxy VLCPUFA lasted for 6 weeks, disappearing with the last spermatids and spermatozoa. The amount per testis of 22:5n-6-rich triacylglycerols, unchanged for 4 weeks, fell between weeks 4-6, associating these lipids with spermatids and their residual bodies, detected as small bright lipid droplets. In contrast, 22:5n-6-rich species of cholesterol esters, and large lipid droplets, increased in seminiferous tubules up to week 6, revealing they are Sertoli cell products. At week 30, the lipid and fatty acid profiles reflected the resulting permanent testicular involution. Our data highlight the importance of Sertoli cells in maintaining lipid homeostasis during normal spermatogenesis.
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Ayuza Aresti, Pablo Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Gigola, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Reyes, Luis E.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Materia
CERAMIDES
CHOLESTEROL ESTERS
LIPID DROPLETS
PHAGOCYTOSIS
POLYUNSATURATED FATTY ACID
SPERMATOGENIC CELL
SERTOLI CELL
SPHINGOMYELIN
TRIACYLGLICEROL
VERY LONG CHAIN PUFA
X-RAYS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/42113
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/42113
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Sequential depletion of rat testicular lipids with long-chain and very long-chain polyenoic fatty acids after X-ray-induced interruption of spermatogenesisOresti, Gerardo MartinAyuza Aresti, Pablo LeonardoGigola, GracielaReyes, Luis E.Aveldaño, Marta IsabelCERAMIDESCHOLESTEROL ESTERSLIPID DROPLETSPHAGOCYTOSISPOLYUNSATURATED FATTY ACIDSPERMATOGENIC CELLSERTOLI CELLSPHINGOMYELINTRIACYLGLICEROLVERY LONG CHAIN PUFAX-RAYShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1When a single dose of X-rays is applied to the adult rat testis, stem spermatogonia are damaged and spermatogenesis is interrupted. Supported by Sertoli cells, those spermatogenic cells that endure irradiation complete their differentiation and gradually leave the testis as spermatozoa. In this study, the in vivo changes taking place a number of weeks after irradiation revealed cell-specific features of testicular lipid classes. A linear drop, taking about 6 weeks, in testis weight, non-lipid materials, free cholesterol and 22:5n-6-rich glycerophospholipids took place with germ cell depletion. Sphingomyelins and ceramides with non-hydroxy very long chain polyenoic fatty acids (n-VLCPUFA) disappeared in 4 weeks, together with the last spermatocytes, whereas species with 2-hydroxy VLCPUFA lasted for 6 weeks, disappearing with the last spermatids and spermatozoa. The amount per testis of 22:5n-6-rich triacylglycerols, unchanged for 4 weeks, fell between weeks 4-6, associating these lipids with spermatids and their residual bodies, detected as small bright lipid droplets. In contrast, 22:5n-6-rich species of cholesterol esters, and large lipid droplets, increased in seminiferous tubules up to week 6, revealing they are Sertoli cell products. At week 30, the lipid and fatty acid profiles reflected the resulting permanent testicular involution. Our data highlight the importance of Sertoli cells in maintaining lipid homeostasis during normal spermatogenesis.Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Ayuza Aresti, Pablo Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Gigola, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Reyes, Luis E.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaAmerican Society for Biochemistry and Molecular Biology2010-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/42113Oresti, Gerardo Martin; Ayuza Aresti, Pablo Leonardo; Gigola, Graciela; Reyes, Luis E.; Aveldaño, Marta Isabel; Sequential depletion of rat testicular lipids with long-chain and very long-chain polyenoic fatty acids after X-ray-induced interruption of spermatogenesis; American Society for Biochemistry and Molecular Biology; Journal of Lipid Research Papers In Press; 51; 9; 9-2010; 2600-26100022-22751539-7262CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.jlr.org/lookup/doi/10.1194/jlr.M006395info:eu-repo/semantics/altIdentifier/doi/10.1194/jlr.M006395info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:07:43Zoai:ri.conicet.gov.ar:11336/42113instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:07:43.613CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Sequential depletion of rat testicular lipids with long-chain and very long-chain polyenoic fatty acids after X-ray-induced interruption of spermatogenesis
title Sequential depletion of rat testicular lipids with long-chain and very long-chain polyenoic fatty acids after X-ray-induced interruption of spermatogenesis
spellingShingle Sequential depletion of rat testicular lipids with long-chain and very long-chain polyenoic fatty acids after X-ray-induced interruption of spermatogenesis
Oresti, Gerardo Martin
CERAMIDES
CHOLESTEROL ESTERS
LIPID DROPLETS
PHAGOCYTOSIS
POLYUNSATURATED FATTY ACID
SPERMATOGENIC CELL
SERTOLI CELL
SPHINGOMYELIN
TRIACYLGLICEROL
VERY LONG CHAIN PUFA
X-RAYS
title_short Sequential depletion of rat testicular lipids with long-chain and very long-chain polyenoic fatty acids after X-ray-induced interruption of spermatogenesis
title_full Sequential depletion of rat testicular lipids with long-chain and very long-chain polyenoic fatty acids after X-ray-induced interruption of spermatogenesis
title_fullStr Sequential depletion of rat testicular lipids with long-chain and very long-chain polyenoic fatty acids after X-ray-induced interruption of spermatogenesis
title_full_unstemmed Sequential depletion of rat testicular lipids with long-chain and very long-chain polyenoic fatty acids after X-ray-induced interruption of spermatogenesis
title_sort Sequential depletion of rat testicular lipids with long-chain and very long-chain polyenoic fatty acids after X-ray-induced interruption of spermatogenesis
dc.creator.none.fl_str_mv Oresti, Gerardo Martin
Ayuza Aresti, Pablo Leonardo
Gigola, Graciela
Reyes, Luis E.
Aveldaño, Marta Isabel
author Oresti, Gerardo Martin
author_facet Oresti, Gerardo Martin
Ayuza Aresti, Pablo Leonardo
Gigola, Graciela
Reyes, Luis E.
Aveldaño, Marta Isabel
author_role author
author2 Ayuza Aresti, Pablo Leonardo
Gigola, Graciela
Reyes, Luis E.
Aveldaño, Marta Isabel
author2_role author
author
author
author
dc.subject.none.fl_str_mv CERAMIDES
CHOLESTEROL ESTERS
LIPID DROPLETS
PHAGOCYTOSIS
POLYUNSATURATED FATTY ACID
SPERMATOGENIC CELL
SERTOLI CELL
SPHINGOMYELIN
TRIACYLGLICEROL
VERY LONG CHAIN PUFA
X-RAYS
topic CERAMIDES
CHOLESTEROL ESTERS
LIPID DROPLETS
PHAGOCYTOSIS
POLYUNSATURATED FATTY ACID
SPERMATOGENIC CELL
SERTOLI CELL
SPHINGOMYELIN
TRIACYLGLICEROL
VERY LONG CHAIN PUFA
X-RAYS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv When a single dose of X-rays is applied to the adult rat testis, stem spermatogonia are damaged and spermatogenesis is interrupted. Supported by Sertoli cells, those spermatogenic cells that endure irradiation complete their differentiation and gradually leave the testis as spermatozoa. In this study, the in vivo changes taking place a number of weeks after irradiation revealed cell-specific features of testicular lipid classes. A linear drop, taking about 6 weeks, in testis weight, non-lipid materials, free cholesterol and 22:5n-6-rich glycerophospholipids took place with germ cell depletion. Sphingomyelins and ceramides with non-hydroxy very long chain polyenoic fatty acids (n-VLCPUFA) disappeared in 4 weeks, together with the last spermatocytes, whereas species with 2-hydroxy VLCPUFA lasted for 6 weeks, disappearing with the last spermatids and spermatozoa. The amount per testis of 22:5n-6-rich triacylglycerols, unchanged for 4 weeks, fell between weeks 4-6, associating these lipids with spermatids and their residual bodies, detected as small bright lipid droplets. In contrast, 22:5n-6-rich species of cholesterol esters, and large lipid droplets, increased in seminiferous tubules up to week 6, revealing they are Sertoli cell products. At week 30, the lipid and fatty acid profiles reflected the resulting permanent testicular involution. Our data highlight the importance of Sertoli cells in maintaining lipid homeostasis during normal spermatogenesis.
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Ayuza Aresti, Pablo Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Gigola, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Reyes, Luis E.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
description When a single dose of X-rays is applied to the adult rat testis, stem spermatogonia are damaged and spermatogenesis is interrupted. Supported by Sertoli cells, those spermatogenic cells that endure irradiation complete their differentiation and gradually leave the testis as spermatozoa. In this study, the in vivo changes taking place a number of weeks after irradiation revealed cell-specific features of testicular lipid classes. A linear drop, taking about 6 weeks, in testis weight, non-lipid materials, free cholesterol and 22:5n-6-rich glycerophospholipids took place with germ cell depletion. Sphingomyelins and ceramides with non-hydroxy very long chain polyenoic fatty acids (n-VLCPUFA) disappeared in 4 weeks, together with the last spermatocytes, whereas species with 2-hydroxy VLCPUFA lasted for 6 weeks, disappearing with the last spermatids and spermatozoa. The amount per testis of 22:5n-6-rich triacylglycerols, unchanged for 4 weeks, fell between weeks 4-6, associating these lipids with spermatids and their residual bodies, detected as small bright lipid droplets. In contrast, 22:5n-6-rich species of cholesterol esters, and large lipid droplets, increased in seminiferous tubules up to week 6, revealing they are Sertoli cell products. At week 30, the lipid and fatty acid profiles reflected the resulting permanent testicular involution. Our data highlight the importance of Sertoli cells in maintaining lipid homeostasis during normal spermatogenesis.
publishDate 2010
dc.date.none.fl_str_mv 2010-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/42113
Oresti, Gerardo Martin; Ayuza Aresti, Pablo Leonardo; Gigola, Graciela; Reyes, Luis E.; Aveldaño, Marta Isabel; Sequential depletion of rat testicular lipids with long-chain and very long-chain polyenoic fatty acids after X-ray-induced interruption of spermatogenesis; American Society for Biochemistry and Molecular Biology; Journal of Lipid Research Papers In Press; 51; 9; 9-2010; 2600-2610
0022-2275
1539-7262
CONICET Digital
CONICET
url http://hdl.handle.net/11336/42113
identifier_str_mv Oresti, Gerardo Martin; Ayuza Aresti, Pablo Leonardo; Gigola, Graciela; Reyes, Luis E.; Aveldaño, Marta Isabel; Sequential depletion of rat testicular lipids with long-chain and very long-chain polyenoic fatty acids after X-ray-induced interruption of spermatogenesis; American Society for Biochemistry and Molecular Biology; Journal of Lipid Research Papers In Press; 51; 9; 9-2010; 2600-2610
0022-2275
1539-7262
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.jlr.org/lookup/doi/10.1194/jlr.M006395
info:eu-repo/semantics/altIdentifier/doi/10.1194/jlr.M006395
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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