NMO-IgG Present in Neuromyelitis Optica Patients Sera Affects AQP4 Expression and Water Permeability of Astrocytes Plasma Membrane

Autores
Melamud, Luciana; Fernandez, Juan; Rivarola, Valeria; Di Giusto, Gisela; Ford, Paula; Villa, Andres; Capurro, Claudia Graciela
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
NMO-IgG autoantibody selectively binds to Aquaporin-4 (AQP4), the most abundant water channel in the central nervous system, and is now considered an useful serum biomarker of neuromyelitis optica (NMO). A series of clinical and pathological observations suggest that NMO-IgG may play a central role in NMO physiopathology. In the current study we evaluated, in well-differentiated astrocytes cultures, the consequences of NMO-IgG binding on the expression pattern of AQP4 and on plasma membrane water permeability. To avoid or to facilitate AQP4 down-regulation cells were exposed to inactivated sera in two different situations (1 hour at 4°C or 12 hours at 37°C). AQP4 expression was detected by immunofluorescence studies, using a polyclonal anti-AQP4 or a human anti-IgG antibody and the water permeability coefficient was evaluated by a videomicroscopy technique. Our results showed that at low temperatures, cell exposure to either control or NMO-IgG sera does not affect neither AQP4 expression nor plasma membrane water permeability, indicating that the simple binding of NMO-IgG does not affect the water channel´s activity. However, at 37 °C, long-term exposure to NMO-IgG induced a loss of human IgG signal from the plasma membrane along with M1-AQP4 isoform removal and a significant reduction of water permeability. These results suggest that binding of NMO-IgG to cell membranes expressing AQP4 is a specific mechanism that may account for at least part of the pathogenic process.
Fil: Melamud, Luciana. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Biomembranas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Centro Universitario de Neurologia "dr. Jose Maria Ramos Mejia".; Argentina
Fil: Fernandez, Juan. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Biomembranas; Argentina
Fil: Rivarola, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Biomembranas; Argentina
Fil: Di Giusto, Gisela. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Biomembranas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ford, Paula. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Biomembranas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Villa, Andres. Universidad de Buenos Aires. Facultad de Medicina. Centro Universitario de Neurologia "dr. Jose Maria Ramos Mejia".; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Biomembranas; Argentina
Fil: Capurro, Claudia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Biomembranas; Argentina
Materia
NEUROMIELITIS OPTICA
ACUAPORINA 4
PERMEABILIDAD AL AGUA
ASTROCITOS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/105642
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling NMO-IgG Present in Neuromyelitis Optica Patients Sera Affects AQP4 Expression and Water Permeability of Astrocytes Plasma MembraneMelamud, LucianaFernandez, JuanRivarola, ValeriaDi Giusto, GiselaFord, PaulaVilla, AndresCapurro, Claudia GracielaNEUROMIELITIS OPTICAACUAPORINA 4PERMEABILIDAD AL AGUAASTROCITOShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3NMO-IgG autoantibody selectively binds to Aquaporin-4 (AQP4), the most abundant water channel in the central nervous system, and is now considered an useful serum biomarker of neuromyelitis optica (NMO). A series of clinical and pathological observations suggest that NMO-IgG may play a central role in NMO physiopathology. In the current study we evaluated, in well-differentiated astrocytes cultures, the consequences of NMO-IgG binding on the expression pattern of AQP4 and on plasma membrane water permeability. To avoid or to facilitate AQP4 down-regulation cells were exposed to inactivated sera in two different situations (1 hour at 4°C or 12 hours at 37°C). AQP4 expression was detected by immunofluorescence studies, using a polyclonal anti-AQP4 or a human anti-IgG antibody and the water permeability coefficient was evaluated by a videomicroscopy technique. Our results showed that at low temperatures, cell exposure to either control or NMO-IgG sera does not affect neither AQP4 expression nor plasma membrane water permeability, indicating that the simple binding of NMO-IgG does not affect the water channel´s activity. However, at 37 °C, long-term exposure to NMO-IgG induced a loss of human IgG signal from the plasma membrane along with M1-AQP4 isoform removal and a significant reduction of water permeability. These results suggest that binding of NMO-IgG to cell membranes expressing AQP4 is a specific mechanism that may account for at least part of the pathogenic process.Fil: Melamud, Luciana. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Biomembranas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Centro Universitario de Neurologia "dr. Jose Maria Ramos Mejia".; ArgentinaFil: Fernandez, Juan. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Biomembranas; ArgentinaFil: Rivarola, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Biomembranas; ArgentinaFil: Di Giusto, Gisela. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Biomembranas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ford, Paula. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Biomembranas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Villa, Andres. Universidad de Buenos Aires. Facultad de Medicina. Centro Universitario de Neurologia "dr. Jose Maria Ramos Mejia".; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Biomembranas; ArgentinaFil: Capurro, Claudia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Biomembranas; ArgentinaWiley-liss, Div John Wiley & Sons Inc2012-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/105642Melamud, Luciana; Fernandez, Juan; Rivarola, Valeria; Di Giusto, Gisela; Ford, Paula; et al.; NMO-IgG Present in Neuromyelitis Optica Patients Sera Affects AQP4 Expression and Water Permeability of Astrocytes Plasma Membrane; Wiley-liss, Div John Wiley & Sons Inc; Journal of Neuroscience Research; 90; 1-2012; 1240-12480360-4012CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/jnr.22822info:eu-repo/semantics/altIdentifier/doi/10.1002/jnr.22822info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:45:58Zoai:ri.conicet.gov.ar:11336/105642instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:45:59.01CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv NMO-IgG Present in Neuromyelitis Optica Patients Sera Affects AQP4 Expression and Water Permeability of Astrocytes Plasma Membrane
title NMO-IgG Present in Neuromyelitis Optica Patients Sera Affects AQP4 Expression and Water Permeability of Astrocytes Plasma Membrane
spellingShingle NMO-IgG Present in Neuromyelitis Optica Patients Sera Affects AQP4 Expression and Water Permeability of Astrocytes Plasma Membrane
Melamud, Luciana
NEUROMIELITIS OPTICA
ACUAPORINA 4
PERMEABILIDAD AL AGUA
ASTROCITOS
title_short NMO-IgG Present in Neuromyelitis Optica Patients Sera Affects AQP4 Expression and Water Permeability of Astrocytes Plasma Membrane
title_full NMO-IgG Present in Neuromyelitis Optica Patients Sera Affects AQP4 Expression and Water Permeability of Astrocytes Plasma Membrane
title_fullStr NMO-IgG Present in Neuromyelitis Optica Patients Sera Affects AQP4 Expression and Water Permeability of Astrocytes Plasma Membrane
title_full_unstemmed NMO-IgG Present in Neuromyelitis Optica Patients Sera Affects AQP4 Expression and Water Permeability of Astrocytes Plasma Membrane
title_sort NMO-IgG Present in Neuromyelitis Optica Patients Sera Affects AQP4 Expression and Water Permeability of Astrocytes Plasma Membrane
dc.creator.none.fl_str_mv Melamud, Luciana
Fernandez, Juan
Rivarola, Valeria
Di Giusto, Gisela
Ford, Paula
Villa, Andres
Capurro, Claudia Graciela
author Melamud, Luciana
author_facet Melamud, Luciana
Fernandez, Juan
Rivarola, Valeria
Di Giusto, Gisela
Ford, Paula
Villa, Andres
Capurro, Claudia Graciela
author_role author
author2 Fernandez, Juan
Rivarola, Valeria
Di Giusto, Gisela
Ford, Paula
Villa, Andres
Capurro, Claudia Graciela
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv NEUROMIELITIS OPTICA
ACUAPORINA 4
PERMEABILIDAD AL AGUA
ASTROCITOS
topic NEUROMIELITIS OPTICA
ACUAPORINA 4
PERMEABILIDAD AL AGUA
ASTROCITOS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv NMO-IgG autoantibody selectively binds to Aquaporin-4 (AQP4), the most abundant water channel in the central nervous system, and is now considered an useful serum biomarker of neuromyelitis optica (NMO). A series of clinical and pathological observations suggest that NMO-IgG may play a central role in NMO physiopathology. In the current study we evaluated, in well-differentiated astrocytes cultures, the consequences of NMO-IgG binding on the expression pattern of AQP4 and on plasma membrane water permeability. To avoid or to facilitate AQP4 down-regulation cells were exposed to inactivated sera in two different situations (1 hour at 4°C or 12 hours at 37°C). AQP4 expression was detected by immunofluorescence studies, using a polyclonal anti-AQP4 or a human anti-IgG antibody and the water permeability coefficient was evaluated by a videomicroscopy technique. Our results showed that at low temperatures, cell exposure to either control or NMO-IgG sera does not affect neither AQP4 expression nor plasma membrane water permeability, indicating that the simple binding of NMO-IgG does not affect the water channel´s activity. However, at 37 °C, long-term exposure to NMO-IgG induced a loss of human IgG signal from the plasma membrane along with M1-AQP4 isoform removal and a significant reduction of water permeability. These results suggest that binding of NMO-IgG to cell membranes expressing AQP4 is a specific mechanism that may account for at least part of the pathogenic process.
Fil: Melamud, Luciana. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Biomembranas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Centro Universitario de Neurologia "dr. Jose Maria Ramos Mejia".; Argentina
Fil: Fernandez, Juan. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Biomembranas; Argentina
Fil: Rivarola, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Biomembranas; Argentina
Fil: Di Giusto, Gisela. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Biomembranas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ford, Paula. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Biomembranas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Villa, Andres. Universidad de Buenos Aires. Facultad de Medicina. Centro Universitario de Neurologia "dr. Jose Maria Ramos Mejia".; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Biomembranas; Argentina
Fil: Capurro, Claudia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Biomembranas; Argentina
description NMO-IgG autoantibody selectively binds to Aquaporin-4 (AQP4), the most abundant water channel in the central nervous system, and is now considered an useful serum biomarker of neuromyelitis optica (NMO). A series of clinical and pathological observations suggest that NMO-IgG may play a central role in NMO physiopathology. In the current study we evaluated, in well-differentiated astrocytes cultures, the consequences of NMO-IgG binding on the expression pattern of AQP4 and on plasma membrane water permeability. To avoid or to facilitate AQP4 down-regulation cells were exposed to inactivated sera in two different situations (1 hour at 4°C or 12 hours at 37°C). AQP4 expression was detected by immunofluorescence studies, using a polyclonal anti-AQP4 or a human anti-IgG antibody and the water permeability coefficient was evaluated by a videomicroscopy technique. Our results showed that at low temperatures, cell exposure to either control or NMO-IgG sera does not affect neither AQP4 expression nor plasma membrane water permeability, indicating that the simple binding of NMO-IgG does not affect the water channel´s activity. However, at 37 °C, long-term exposure to NMO-IgG induced a loss of human IgG signal from the plasma membrane along with M1-AQP4 isoform removal and a significant reduction of water permeability. These results suggest that binding of NMO-IgG to cell membranes expressing AQP4 is a specific mechanism that may account for at least part of the pathogenic process.
publishDate 2012
dc.date.none.fl_str_mv 2012-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/105642
Melamud, Luciana; Fernandez, Juan; Rivarola, Valeria; Di Giusto, Gisela; Ford, Paula; et al.; NMO-IgG Present in Neuromyelitis Optica Patients Sera Affects AQP4 Expression and Water Permeability of Astrocytes Plasma Membrane; Wiley-liss, Div John Wiley & Sons Inc; Journal of Neuroscience Research; 90; 1-2012; 1240-1248
0360-4012
CONICET Digital
CONICET
url http://hdl.handle.net/11336/105642
identifier_str_mv Melamud, Luciana; Fernandez, Juan; Rivarola, Valeria; Di Giusto, Gisela; Ford, Paula; et al.; NMO-IgG Present in Neuromyelitis Optica Patients Sera Affects AQP4 Expression and Water Permeability of Astrocytes Plasma Membrane; Wiley-liss, Div John Wiley & Sons Inc; Journal of Neuroscience Research; 90; 1-2012; 1240-1248
0360-4012
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/jnr.22822
info:eu-repo/semantics/altIdentifier/doi/10.1002/jnr.22822
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley-liss, Div John Wiley & Sons Inc
publisher.none.fl_str_mv Wiley-liss, Div John Wiley & Sons Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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