Deimination level and peptidyl arginine deiminase 2 expression are elevated in astrocytes with increased incubation temperature

Autores
Enriquez Algeciras, Mabel; Bhattacharya, Sanjoy K.; Serra, Horacio Marcelo
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Astrocytes respond to environmental cues, including changes in temperatures. Increased deimination, observed in many progressive neurological diseases, is thought to be contributed by astrocytes. We determined the level of deimination and expression of peptidyl arginine deiminase 2 (PAD2) in isolated primary astrocytes in response to changes on either side (31°C and 41°C) of the optimal temperature (37°C). We investigated changes in the astrocytes by using a number of established markers and accounted for cell death with the CellTiter‐Blue assay. We found increased expression of glial fibrillary acidic protein, ALDH1L1, and J1‐31, resulting from increased incubation temperature and increased expression of TSP1, S100β, and AQP4, resulting from decreased incubation temperature vs. optimal temperature, suggesting activation of different biochemical pathways in astrocytes associated with different incubation temperatures. Mass spectrometric analyses support such trends. The PAD2 level was increased only as a result of increased incubation temperature with a commensurate increased level of deimination. Actin cytoskeleton and iso[4]LGE, a lipid peroxidase modification, also showed an increase with higher incubation temperature. Altogether, these results suggest that temperature, as an environmental cue, activates astrocytes in a different manner on either side of the optimal temperature and that increase in deimination is associated only with the higher temperature side of the spectrum.
Fil: Enriquez Algeciras, Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Miami University; Estados Unidos
Fil: Bhattacharya, Sanjoy K.. Miami University; Estados Unidos
Fil: Serra, Horacio Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Materia
Astrocitos
Pad
Glaucoma
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/46047
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/46047
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Deimination level and peptidyl arginine deiminase 2 expression are elevated in astrocytes with increased incubation temperatureEnriquez Algeciras, MabelBhattacharya, Sanjoy K.Serra, Horacio MarceloAstrocitosPadGlaucomahttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Astrocytes respond to environmental cues, including changes in temperatures. Increased deimination, observed in many progressive neurological diseases, is thought to be contributed by astrocytes. We determined the level of deimination and expression of peptidyl arginine deiminase 2 (PAD2) in isolated primary astrocytes in response to changes on either side (31°C and 41°C) of the optimal temperature (37°C). We investigated changes in the astrocytes by using a number of established markers and accounted for cell death with the CellTiter‐Blue assay. We found increased expression of glial fibrillary acidic protein, ALDH1L1, and J1‐31, resulting from increased incubation temperature and increased expression of TSP1, S100β, and AQP4, resulting from decreased incubation temperature vs. optimal temperature, suggesting activation of different biochemical pathways in astrocytes associated with different incubation temperatures. Mass spectrometric analyses support such trends. The PAD2 level was increased only as a result of increased incubation temperature with a commensurate increased level of deimination. Actin cytoskeleton and iso[4]LGE, a lipid peroxidase modification, also showed an increase with higher incubation temperature. Altogether, these results suggest that temperature, as an environmental cue, activates astrocytes in a different manner on either side of the optimal temperature and that increase in deimination is associated only with the higher temperature side of the spectrum.Fil: Enriquez Algeciras, Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Miami University; Estados UnidosFil: Bhattacharya, Sanjoy K.. Miami University; Estados UnidosFil: Serra, Horacio Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaWiley-liss, Div John Wiley & Sons Inc2015-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/46047Enriquez Algeciras, Mabel; Bhattacharya, Sanjoy K.; Serra, Horacio Marcelo; Deimination level and peptidyl arginine deiminase 2 expression are elevated in astrocytes with increased incubation temperature; Wiley-liss, Div John Wiley & Sons Inc; Journal of Neuroscience Research; 93; 9; 9-2015; 1388-13980360-40121097-4547CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://bit.ly/2II2n5Oinfo:eu-repo/semantics/altIdentifier/doi/10.1002/jnr.23587info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:40:53Zoai:ri.conicet.gov.ar:11336/46047instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:40:53.999CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Deimination level and peptidyl arginine deiminase 2 expression are elevated in astrocytes with increased incubation temperature
title Deimination level and peptidyl arginine deiminase 2 expression are elevated in astrocytes with increased incubation temperature
spellingShingle Deimination level and peptidyl arginine deiminase 2 expression are elevated in astrocytes with increased incubation temperature
Enriquez Algeciras, Mabel
Astrocitos
Pad
Glaucoma
title_short Deimination level and peptidyl arginine deiminase 2 expression are elevated in astrocytes with increased incubation temperature
title_full Deimination level and peptidyl arginine deiminase 2 expression are elevated in astrocytes with increased incubation temperature
title_fullStr Deimination level and peptidyl arginine deiminase 2 expression are elevated in astrocytes with increased incubation temperature
title_full_unstemmed Deimination level and peptidyl arginine deiminase 2 expression are elevated in astrocytes with increased incubation temperature
title_sort Deimination level and peptidyl arginine deiminase 2 expression are elevated in astrocytes with increased incubation temperature
dc.creator.none.fl_str_mv Enriquez Algeciras, Mabel
Bhattacharya, Sanjoy K.
Serra, Horacio Marcelo
author Enriquez Algeciras, Mabel
author_facet Enriquez Algeciras, Mabel
Bhattacharya, Sanjoy K.
Serra, Horacio Marcelo
author_role author
author2 Bhattacharya, Sanjoy K.
Serra, Horacio Marcelo
author2_role author
author
dc.subject.none.fl_str_mv Astrocitos
Pad
Glaucoma
topic Astrocitos
Pad
Glaucoma
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Astrocytes respond to environmental cues, including changes in temperatures. Increased deimination, observed in many progressive neurological diseases, is thought to be contributed by astrocytes. We determined the level of deimination and expression of peptidyl arginine deiminase 2 (PAD2) in isolated primary astrocytes in response to changes on either side (31°C and 41°C) of the optimal temperature (37°C). We investigated changes in the astrocytes by using a number of established markers and accounted for cell death with the CellTiter‐Blue assay. We found increased expression of glial fibrillary acidic protein, ALDH1L1, and J1‐31, resulting from increased incubation temperature and increased expression of TSP1, S100β, and AQP4, resulting from decreased incubation temperature vs. optimal temperature, suggesting activation of different biochemical pathways in astrocytes associated with different incubation temperatures. Mass spectrometric analyses support such trends. The PAD2 level was increased only as a result of increased incubation temperature with a commensurate increased level of deimination. Actin cytoskeleton and iso[4]LGE, a lipid peroxidase modification, also showed an increase with higher incubation temperature. Altogether, these results suggest that temperature, as an environmental cue, activates astrocytes in a different manner on either side of the optimal temperature and that increase in deimination is associated only with the higher temperature side of the spectrum.
Fil: Enriquez Algeciras, Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Miami University; Estados Unidos
Fil: Bhattacharya, Sanjoy K.. Miami University; Estados Unidos
Fil: Serra, Horacio Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
description Astrocytes respond to environmental cues, including changes in temperatures. Increased deimination, observed in many progressive neurological diseases, is thought to be contributed by astrocytes. We determined the level of deimination and expression of peptidyl arginine deiminase 2 (PAD2) in isolated primary astrocytes in response to changes on either side (31°C and 41°C) of the optimal temperature (37°C). We investigated changes in the astrocytes by using a number of established markers and accounted for cell death with the CellTiter‐Blue assay. We found increased expression of glial fibrillary acidic protein, ALDH1L1, and J1‐31, resulting from increased incubation temperature and increased expression of TSP1, S100β, and AQP4, resulting from decreased incubation temperature vs. optimal temperature, suggesting activation of different biochemical pathways in astrocytes associated with different incubation temperatures. Mass spectrometric analyses support such trends. The PAD2 level was increased only as a result of increased incubation temperature with a commensurate increased level of deimination. Actin cytoskeleton and iso[4]LGE, a lipid peroxidase modification, also showed an increase with higher incubation temperature. Altogether, these results suggest that temperature, as an environmental cue, activates astrocytes in a different manner on either side of the optimal temperature and that increase in deimination is associated only with the higher temperature side of the spectrum.
publishDate 2015
dc.date.none.fl_str_mv 2015-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/46047
Enriquez Algeciras, Mabel; Bhattacharya, Sanjoy K.; Serra, Horacio Marcelo; Deimination level and peptidyl arginine deiminase 2 expression are elevated in astrocytes with increased incubation temperature; Wiley-liss, Div John Wiley & Sons Inc; Journal of Neuroscience Research; 93; 9; 9-2015; 1388-1398
0360-4012
1097-4547
CONICET Digital
CONICET
url http://hdl.handle.net/11336/46047
identifier_str_mv Enriquez Algeciras, Mabel; Bhattacharya, Sanjoy K.; Serra, Horacio Marcelo; Deimination level and peptidyl arginine deiminase 2 expression are elevated in astrocytes with increased incubation temperature; Wiley-liss, Div John Wiley & Sons Inc; Journal of Neuroscience Research; 93; 9; 9-2015; 1388-1398
0360-4012
1097-4547
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://bit.ly/2II2n5O
info:eu-repo/semantics/altIdentifier/doi/10.1002/jnr.23587
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley-liss, Div John Wiley & Sons Inc
publisher.none.fl_str_mv Wiley-liss, Div John Wiley & Sons Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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