Advancing ocular safety research: A comprehensive examination of benzocaine acute exposure without animal testing

Autores
Lenze, Mariela Belén; Benedetti, Martina Daniela; Roco, Julieta; Ramírez, Pedro Germán; Blanco, Raúl; Yaceszen, Sol; Corrales, Carlos; Wikinski, Silvia Ines; Gutiérrez, María Laura
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Benzocaine is a widely employed local anaesthetic; however, there is a notable dearth of preclinical and clinical evidence regarding its safety in ophthalmological products. To address this, a comprehensive strategy incorporating in silico and in vitro methodologies was proposed for assessing benzocaine´s ocular toxicity without animal testing. To collect the in silico evidence, the QSAR Toolbox (v4.5) was used. A single exposure to two benzocaine concentrations (2% and 20%) was evaluated by in vitro methods. Hen’s Egg Chorioallantoic Membrane Test (HET-CAM) was performed to evaluate the effects on the conjunctiva. To study corneal integrity, Short Time Exposure test (STE) and Bovine Corneal Opacity and Permeability (BCOP) assay, followed by histopathological analysis, were carried out. Results from both in silico and in vitro methodologies categorize benzocaine as non-irritating. The histopathological analysis further affirms the safety of using benzocaine in eye drops, as no alterations were observed in evaluated corneal strata. This research proposes a useful combined strategy to provide evidence on the safety of local anaesthetics and particularly show that 2% and 20% benzocaine solutions do not induce eye irritation or corneal damage, supporting the potential use of benzocaine in the development of ophthalmic anesthetic products.
Fil: Lenze, Mariela Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacologia; Argentina
Fil: Benedetti, Martina Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacologia; Argentina
Fil: Roco, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacologia; Argentina
Fil: Ramírez, Pedro Germán. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacologia; Argentina
Fil: Blanco, Raúl. Laboratorio Labyes; Argentina
Fil: Yaceszen, Sol. Laboratorio Labyes; Argentina
Fil: Corrales, Carlos. Laboratorio Labyes; Argentina
Fil: Wikinski, Silvia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacologia; Argentina
Fil: Gutiérrez, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacologia; Argentina
Materia
Benzocaine
Ocular
Safety
Toxicology
Nivel de accesibilidad
acceso embargado
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/232272
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/232272
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Advancing ocular safety research: A comprehensive examination of benzocaine acute exposure without animal testingLenze, Mariela BelénBenedetti, Martina DanielaRoco, JulietaRamírez, Pedro GermánBlanco, RaúlYaceszen, SolCorrales, CarlosWikinski, Silvia InesGutiérrez, María LauraBenzocaineOcularSafetyToxicologyhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Benzocaine is a widely employed local anaesthetic; however, there is a notable dearth of preclinical and clinical evidence regarding its safety in ophthalmological products. To address this, a comprehensive strategy incorporating in silico and in vitro methodologies was proposed for assessing benzocaine´s ocular toxicity without animal testing. To collect the in silico evidence, the QSAR Toolbox (v4.5) was used. A single exposure to two benzocaine concentrations (2% and 20%) was evaluated by in vitro methods. Hen’s Egg Chorioallantoic Membrane Test (HET-CAM) was performed to evaluate the effects on the conjunctiva. To study corneal integrity, Short Time Exposure test (STE) and Bovine Corneal Opacity and Permeability (BCOP) assay, followed by histopathological analysis, were carried out. Results from both in silico and in vitro methodologies categorize benzocaine as non-irritating. The histopathological analysis further affirms the safety of using benzocaine in eye drops, as no alterations were observed in evaluated corneal strata. This research proposes a useful combined strategy to provide evidence on the safety of local anaesthetics and particularly show that 2% and 20% benzocaine solutions do not induce eye irritation or corneal damage, supporting the potential use of benzocaine in the development of ophthalmic anesthetic products.Fil: Lenze, Mariela Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacologia; ArgentinaFil: Benedetti, Martina Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacologia; ArgentinaFil: Roco, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacologia; ArgentinaFil: Ramírez, Pedro Germán. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacologia; ArgentinaFil: Blanco, Raúl. Laboratorio Labyes; ArgentinaFil: Yaceszen, Sol. Laboratorio Labyes; ArgentinaFil: Corrales, Carlos. Laboratorio Labyes; ArgentinaFil: Wikinski, Silvia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacologia; ArgentinaFil: Gutiérrez, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacologia; ArgentinaElsevier Ireland2024-03info:eu-repo/date/embargoEnd/2024-10-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/232272Lenze, Mariela Belén; Benedetti, Martina Daniela; Roco, Julieta; Ramírez, Pedro Germán; Blanco, Raúl; et al.; Advancing ocular safety research: A comprehensive examination of benzocaine acute exposure without animal testing; Elsevier Ireland; Toxicology Letters; 394; 3-2024; 138-1450378-42741879-3169CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0378427424000511info:eu-repo/semantics/altIdentifier/doi/10.1016/j.toxlet.2024.03.003info:eu-repo/semantics/embargoedAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:15:06Zoai:ri.conicet.gov.ar:11336/232272instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:15:06.723CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Advancing ocular safety research: A comprehensive examination of benzocaine acute exposure without animal testing
title Advancing ocular safety research: A comprehensive examination of benzocaine acute exposure without animal testing
spellingShingle Advancing ocular safety research: A comprehensive examination of benzocaine acute exposure without animal testing
Lenze, Mariela Belén
Benzocaine
Ocular
Safety
Toxicology
title_short Advancing ocular safety research: A comprehensive examination of benzocaine acute exposure without animal testing
title_full Advancing ocular safety research: A comprehensive examination of benzocaine acute exposure without animal testing
title_fullStr Advancing ocular safety research: A comprehensive examination of benzocaine acute exposure without animal testing
title_full_unstemmed Advancing ocular safety research: A comprehensive examination of benzocaine acute exposure without animal testing
title_sort Advancing ocular safety research: A comprehensive examination of benzocaine acute exposure without animal testing
dc.creator.none.fl_str_mv Lenze, Mariela Belén
Benedetti, Martina Daniela
Roco, Julieta
Ramírez, Pedro Germán
Blanco, Raúl
Yaceszen, Sol
Corrales, Carlos
Wikinski, Silvia Ines
Gutiérrez, María Laura
author Lenze, Mariela Belén
author_facet Lenze, Mariela Belén
Benedetti, Martina Daniela
Roco, Julieta
Ramírez, Pedro Germán
Blanco, Raúl
Yaceszen, Sol
Corrales, Carlos
Wikinski, Silvia Ines
Gutiérrez, María Laura
author_role author
author2 Benedetti, Martina Daniela
Roco, Julieta
Ramírez, Pedro Germán
Blanco, Raúl
Yaceszen, Sol
Corrales, Carlos
Wikinski, Silvia Ines
Gutiérrez, María Laura
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Benzocaine
Ocular
Safety
Toxicology
topic Benzocaine
Ocular
Safety
Toxicology
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Benzocaine is a widely employed local anaesthetic; however, there is a notable dearth of preclinical and clinical evidence regarding its safety in ophthalmological products. To address this, a comprehensive strategy incorporating in silico and in vitro methodologies was proposed for assessing benzocaine´s ocular toxicity without animal testing. To collect the in silico evidence, the QSAR Toolbox (v4.5) was used. A single exposure to two benzocaine concentrations (2% and 20%) was evaluated by in vitro methods. Hen’s Egg Chorioallantoic Membrane Test (HET-CAM) was performed to evaluate the effects on the conjunctiva. To study corneal integrity, Short Time Exposure test (STE) and Bovine Corneal Opacity and Permeability (BCOP) assay, followed by histopathological analysis, were carried out. Results from both in silico and in vitro methodologies categorize benzocaine as non-irritating. The histopathological analysis further affirms the safety of using benzocaine in eye drops, as no alterations were observed in evaluated corneal strata. This research proposes a useful combined strategy to provide evidence on the safety of local anaesthetics and particularly show that 2% and 20% benzocaine solutions do not induce eye irritation or corneal damage, supporting the potential use of benzocaine in the development of ophthalmic anesthetic products.
Fil: Lenze, Mariela Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacologia; Argentina
Fil: Benedetti, Martina Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacologia; Argentina
Fil: Roco, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacologia; Argentina
Fil: Ramírez, Pedro Germán. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacologia; Argentina
Fil: Blanco, Raúl. Laboratorio Labyes; Argentina
Fil: Yaceszen, Sol. Laboratorio Labyes; Argentina
Fil: Corrales, Carlos. Laboratorio Labyes; Argentina
Fil: Wikinski, Silvia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacologia; Argentina
Fil: Gutiérrez, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacologia; Argentina
description Benzocaine is a widely employed local anaesthetic; however, there is a notable dearth of preclinical and clinical evidence regarding its safety in ophthalmological products. To address this, a comprehensive strategy incorporating in silico and in vitro methodologies was proposed for assessing benzocaine´s ocular toxicity without animal testing. To collect the in silico evidence, the QSAR Toolbox (v4.5) was used. A single exposure to two benzocaine concentrations (2% and 20%) was evaluated by in vitro methods. Hen’s Egg Chorioallantoic Membrane Test (HET-CAM) was performed to evaluate the effects on the conjunctiva. To study corneal integrity, Short Time Exposure test (STE) and Bovine Corneal Opacity and Permeability (BCOP) assay, followed by histopathological analysis, were carried out. Results from both in silico and in vitro methodologies categorize benzocaine as non-irritating. The histopathological analysis further affirms the safety of using benzocaine in eye drops, as no alterations were observed in evaluated corneal strata. This research proposes a useful combined strategy to provide evidence on the safety of local anaesthetics and particularly show that 2% and 20% benzocaine solutions do not induce eye irritation or corneal damage, supporting the potential use of benzocaine in the development of ophthalmic anesthetic products.
publishDate 2024
dc.date.none.fl_str_mv 2024-03
info:eu-repo/date/embargoEnd/2024-10-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/232272
Lenze, Mariela Belén; Benedetti, Martina Daniela; Roco, Julieta; Ramírez, Pedro Germán; Blanco, Raúl; et al.; Advancing ocular safety research: A comprehensive examination of benzocaine acute exposure without animal testing; Elsevier Ireland; Toxicology Letters; 394; 3-2024; 138-145
0378-4274
1879-3169
CONICET Digital
CONICET
url http://hdl.handle.net/11336/232272
identifier_str_mv Lenze, Mariela Belén; Benedetti, Martina Daniela; Roco, Julieta; Ramírez, Pedro Germán; Blanco, Raúl; et al.; Advancing ocular safety research: A comprehensive examination of benzocaine acute exposure without animal testing; Elsevier Ireland; Toxicology Letters; 394; 3-2024; 138-145
0378-4274
1879-3169
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0378427424000511
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.toxlet.2024.03.003
dc.rights.none.fl_str_mv info:eu-repo/semantics/embargoedAccess
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eu_rights_str_mv embargoedAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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