Ruminal bacterial community changes during adaptation of goats to fresh alfalfa forage

Autores
Grilli, Diego Javier; Mrázek, Jakub; Fliegerová, Katerina; Kopečný, Jan; Paez Lama, Sebastián Antonio; Cerón Cucchi, María Esperanza; Sosa Escudero, Miguel Angel; Arenas, Graciela Nora
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Using culture-independent molecular approaches, we studied the bacterial population changes in the rumen of goats abruptly converted from alfalfa hay to fresh alfalfa diet. Administration of fresh forage with significantly increased soluble nitrogen and soluble protein nitrogen resulted in frothy bloat. Changes of the bacterial composition of rumen were monitored using DGGE analysis of 16S rDNA gene amplicons and quantitative PCR method. As the diet changed, the bacterial population of Bacteroidetes and γ-Proteobacteria decreased, even if animals have not shown signs of frothy bloat. The most severely bloated animals showed an increase of Bacteroidetes phylum. Lactobacillus/Streptococcus group belonging to Firmicutes phylum decreased in response to transferring the animals from hay to a fresh forage-based diet, and did not achieve the values observed at the beginning of the experiment. In summary, changes in the diet and subsequent frothy bloat occurrence produce long-lasting changes in the structure of the microbial community and may be associated with a specific bacterial population belonging to the Bacteroidetes phylum.
Fil: Grilli, Diego Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad "Juan Agustín Maza"; Argentina
Fil: Mrázek, Jakub. Institute Of Animal Physiology And Genetics.; República Checa
Fil: Fliegerová, Katerina. Institute Of Animal Physiology And Genetics.; República Checa
Fil: Kopečný, Jan. Institute Of Animal Physiology And Genetics.; República Checa
Fil: Paez Lama, Sebastián Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Provincia de Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas; Argentina
Fil: Cerón Cucchi, María Esperanza. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Sosa Escudero, Miguel Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Arenas, Graciela Nora. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Materia
BACTERIA
CREOLE GOATS
PCR-DGGE
QPCR
RUMEN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/44659
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Ruminal bacterial community changes during adaptation of goats to fresh alfalfa forageGrilli, Diego JavierMrázek, JakubFliegerová, KaterinaKopečný, JanPaez Lama, Sebastián AntonioCerón Cucchi, María EsperanzaSosa Escudero, Miguel AngelArenas, Graciela NoraBACTERIACREOLE GOATSPCR-DGGEQPCRRUMENhttps://purl.org/becyt/ford/4.2https://purl.org/becyt/ford/4Using culture-independent molecular approaches, we studied the bacterial population changes in the rumen of goats abruptly converted from alfalfa hay to fresh alfalfa diet. Administration of fresh forage with significantly increased soluble nitrogen and soluble protein nitrogen resulted in frothy bloat. Changes of the bacterial composition of rumen were monitored using DGGE analysis of 16S rDNA gene amplicons and quantitative PCR method. As the diet changed, the bacterial population of Bacteroidetes and γ-Proteobacteria decreased, even if animals have not shown signs of frothy bloat. The most severely bloated animals showed an increase of Bacteroidetes phylum. <em>Lactobacillus/Streptococcus</em> group belonging to Firmicutes phylum decreased in response to transferring the animals from hay to a fresh forage-based diet, and did not achieve the values observed at the beginning of the experiment. In summary, changes in the diet and subsequent frothy bloat occurrence produce long-lasting changes in the structure of the microbial community and may be associated with a specific bacterial population belonging to the Bacteroidetes phylum.Fil: Grilli, Diego Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad "Juan Agustín Maza"; ArgentinaFil: Mrázek, Jakub. Institute Of Animal Physiology And Genetics.; República ChecaFil: Fliegerová, Katerina. Institute Of Animal Physiology And Genetics.; República ChecaFil: Kopečný, Jan. Institute Of Animal Physiology And Genetics.; República ChecaFil: Paez Lama, Sebastián Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Provincia de Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas; ArgentinaFil: Cerón Cucchi, María Esperanza. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Sosa Escudero, Miguel Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Arenas, Graciela Nora. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; ArgentinaElsevier Science2016-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/44659Grilli, Diego Javier; Mrázek, Jakub; Fliegerová, Katerina; Kopečný, Jan; Paez Lama, Sebastián Antonio; et al.; Ruminal bacterial community changes during adaptation of goats to fresh alfalfa forage; Elsevier Science; Livestock Science; 191; 9-2016; 191-1951871-1413CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S187114131630172Xinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.livsci.2016.08.001info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:51:15Zoai:ri.conicet.gov.ar:11336/44659instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:51:15.307CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Ruminal bacterial community changes during adaptation of goats to fresh alfalfa forage
title Ruminal bacterial community changes during adaptation of goats to fresh alfalfa forage
spellingShingle Ruminal bacterial community changes during adaptation of goats to fresh alfalfa forage
Grilli, Diego Javier
BACTERIA
CREOLE GOATS
PCR-DGGE
QPCR
RUMEN
title_short Ruminal bacterial community changes during adaptation of goats to fresh alfalfa forage
title_full Ruminal bacterial community changes during adaptation of goats to fresh alfalfa forage
title_fullStr Ruminal bacterial community changes during adaptation of goats to fresh alfalfa forage
title_full_unstemmed Ruminal bacterial community changes during adaptation of goats to fresh alfalfa forage
title_sort Ruminal bacterial community changes during adaptation of goats to fresh alfalfa forage
dc.creator.none.fl_str_mv Grilli, Diego Javier
Mrázek, Jakub
Fliegerová, Katerina
Kopečný, Jan
Paez Lama, Sebastián Antonio
Cerón Cucchi, María Esperanza
Sosa Escudero, Miguel Angel
Arenas, Graciela Nora
author Grilli, Diego Javier
author_facet Grilli, Diego Javier
Mrázek, Jakub
Fliegerová, Katerina
Kopečný, Jan
Paez Lama, Sebastián Antonio
Cerón Cucchi, María Esperanza
Sosa Escudero, Miguel Angel
Arenas, Graciela Nora
author_role author
author2 Mrázek, Jakub
Fliegerová, Katerina
Kopečný, Jan
Paez Lama, Sebastián Antonio
Cerón Cucchi, María Esperanza
Sosa Escudero, Miguel Angel
Arenas, Graciela Nora
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BACTERIA
CREOLE GOATS
PCR-DGGE
QPCR
RUMEN
topic BACTERIA
CREOLE GOATS
PCR-DGGE
QPCR
RUMEN
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.2
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Using culture-independent molecular approaches, we studied the bacterial population changes in the rumen of goats abruptly converted from alfalfa hay to fresh alfalfa diet. Administration of fresh forage with significantly increased soluble nitrogen and soluble protein nitrogen resulted in frothy bloat. Changes of the bacterial composition of rumen were monitored using DGGE analysis of 16S rDNA gene amplicons and quantitative PCR method. As the diet changed, the bacterial population of Bacteroidetes and γ-Proteobacteria decreased, even if animals have not shown signs of frothy bloat. The most severely bloated animals showed an increase of Bacteroidetes phylum. <em>Lactobacillus/Streptococcus</em> group belonging to Firmicutes phylum decreased in response to transferring the animals from hay to a fresh forage-based diet, and did not achieve the values observed at the beginning of the experiment. In summary, changes in the diet and subsequent frothy bloat occurrence produce long-lasting changes in the structure of the microbial community and may be associated with a specific bacterial population belonging to the Bacteroidetes phylum.
Fil: Grilli, Diego Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad "Juan Agustín Maza"; Argentina
Fil: Mrázek, Jakub. Institute Of Animal Physiology And Genetics.; República Checa
Fil: Fliegerová, Katerina. Institute Of Animal Physiology And Genetics.; República Checa
Fil: Kopečný, Jan. Institute Of Animal Physiology And Genetics.; República Checa
Fil: Paez Lama, Sebastián Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Provincia de Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas; Argentina
Fil: Cerón Cucchi, María Esperanza. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Sosa Escudero, Miguel Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Arenas, Graciela Nora. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
description Using culture-independent molecular approaches, we studied the bacterial population changes in the rumen of goats abruptly converted from alfalfa hay to fresh alfalfa diet. Administration of fresh forage with significantly increased soluble nitrogen and soluble protein nitrogen resulted in frothy bloat. Changes of the bacterial composition of rumen were monitored using DGGE analysis of 16S rDNA gene amplicons and quantitative PCR method. As the diet changed, the bacterial population of Bacteroidetes and γ-Proteobacteria decreased, even if animals have not shown signs of frothy bloat. The most severely bloated animals showed an increase of Bacteroidetes phylum. <em>Lactobacillus/Streptococcus</em> group belonging to Firmicutes phylum decreased in response to transferring the animals from hay to a fresh forage-based diet, and did not achieve the values observed at the beginning of the experiment. In summary, changes in the diet and subsequent frothy bloat occurrence produce long-lasting changes in the structure of the microbial community and may be associated with a specific bacterial population belonging to the Bacteroidetes phylum.
publishDate 2016
dc.date.none.fl_str_mv 2016-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/44659
Grilli, Diego Javier; Mrázek, Jakub; Fliegerová, Katerina; Kopečný, Jan; Paez Lama, Sebastián Antonio; et al.; Ruminal bacterial community changes during adaptation of goats to fresh alfalfa forage; Elsevier Science; Livestock Science; 191; 9-2016; 191-195
1871-1413
CONICET Digital
CONICET
url http://hdl.handle.net/11336/44659
identifier_str_mv Grilli, Diego Javier; Mrázek, Jakub; Fliegerová, Katerina; Kopečný, Jan; Paez Lama, Sebastián Antonio; et al.; Ruminal bacterial community changes during adaptation of goats to fresh alfalfa forage; Elsevier Science; Livestock Science; 191; 9-2016; 191-195
1871-1413
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S187114131630172X
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.livsci.2016.08.001
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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