Direct molecular approach to monitoring bacterial colonization on vacuum-packaged beef

Autores
Fontana, Cecilia Alejandra; Cocconcelli, Pier Sandro; Vignolo, Graciela Margarita
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Denaturing gradient gel electrophoresis allowed us to monitor total bacterial communities and to establish a pattern of succession between species in vacuum-packaged beef stored at 2 and 8°C for 9 weeks and 14 days. Species-specific PCR was used to confirm the presence of Lactobacillus sakei and Lactobacillus curvatus. Multiplex PCRs using 16S rRNA-specific primers allowed differentiation between Leuconostoc species. These methods provided the desired information about microbial diversity by detecting the main microorganisms capable of colonizing this ecological niche. Copyright © 2006, American Society for Microbiology. All Rights Reserved.
Fil: Fontana, Cecilia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Cocconcelli, Pier Sandro. Università Cattolica del Sacro Cuore; Italia
Fil: Vignolo, Graciela Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Materia
VACUUM-PACKAGED BEEF
DGGE
LACTIC ACID BACTERIA
SPECIES SPECIFIC PCR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/55405
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Direct molecular approach to monitoring bacterial colonization on vacuum-packaged beefFontana, Cecilia AlejandraCocconcelli, Pier SandroVignolo, Graciela MargaritaVACUUM-PACKAGED BEEFDGGELACTIC ACID BACTERIASPECIES SPECIFIC PCRhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Denaturing gradient gel electrophoresis allowed us to monitor total bacterial communities and to establish a pattern of succession between species in vacuum-packaged beef stored at 2 and 8°C for 9 weeks and 14 days. Species-specific PCR was used to confirm the presence of Lactobacillus sakei and Lactobacillus curvatus. Multiplex PCRs using 16S rRNA-specific primers allowed differentiation between Leuconostoc species. These methods provided the desired information about microbial diversity by detecting the main microorganisms capable of colonizing this ecological niche. Copyright © 2006, American Society for Microbiology. All Rights Reserved.Fil: Fontana, Cecilia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Cocconcelli, Pier Sandro. Università Cattolica del Sacro Cuore; ItaliaFil: Vignolo, Graciela Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaAmerican Society for Microbiology2006-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/55405Fontana, Cecilia Alejandra; Cocconcelli, Pier Sandro; Vignolo, Graciela Margarita; Direct molecular approach to monitoring bacterial colonization on vacuum-packaged beef; American Society for Microbiology; Applied And Environmental Microbiology; 72; 8; 8-2006; 5618-56220099-2240CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1128/AEM.00029-06info:eu-repo/semantics/altIdentifier/url/http://aem.asm.org/content/72/8/5618info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:55:15Zoai:ri.conicet.gov.ar:11336/55405instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:55:15.464CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Direct molecular approach to monitoring bacterial colonization on vacuum-packaged beef
title Direct molecular approach to monitoring bacterial colonization on vacuum-packaged beef
spellingShingle Direct molecular approach to monitoring bacterial colonization on vacuum-packaged beef
Fontana, Cecilia Alejandra
VACUUM-PACKAGED BEEF
DGGE
LACTIC ACID BACTERIA
SPECIES SPECIFIC PCR
title_short Direct molecular approach to monitoring bacterial colonization on vacuum-packaged beef
title_full Direct molecular approach to monitoring bacterial colonization on vacuum-packaged beef
title_fullStr Direct molecular approach to monitoring bacterial colonization on vacuum-packaged beef
title_full_unstemmed Direct molecular approach to monitoring bacterial colonization on vacuum-packaged beef
title_sort Direct molecular approach to monitoring bacterial colonization on vacuum-packaged beef
dc.creator.none.fl_str_mv Fontana, Cecilia Alejandra
Cocconcelli, Pier Sandro
Vignolo, Graciela Margarita
author Fontana, Cecilia Alejandra
author_facet Fontana, Cecilia Alejandra
Cocconcelli, Pier Sandro
Vignolo, Graciela Margarita
author_role author
author2 Cocconcelli, Pier Sandro
Vignolo, Graciela Margarita
author2_role author
author
dc.subject.none.fl_str_mv VACUUM-PACKAGED BEEF
DGGE
LACTIC ACID BACTERIA
SPECIES SPECIFIC PCR
topic VACUUM-PACKAGED BEEF
DGGE
LACTIC ACID BACTERIA
SPECIES SPECIFIC PCR
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Denaturing gradient gel electrophoresis allowed us to monitor total bacterial communities and to establish a pattern of succession between species in vacuum-packaged beef stored at 2 and 8°C for 9 weeks and 14 days. Species-specific PCR was used to confirm the presence of Lactobacillus sakei and Lactobacillus curvatus. Multiplex PCRs using 16S rRNA-specific primers allowed differentiation between Leuconostoc species. These methods provided the desired information about microbial diversity by detecting the main microorganisms capable of colonizing this ecological niche. Copyright © 2006, American Society for Microbiology. All Rights Reserved.
Fil: Fontana, Cecilia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Cocconcelli, Pier Sandro. Università Cattolica del Sacro Cuore; Italia
Fil: Vignolo, Graciela Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
description Denaturing gradient gel electrophoresis allowed us to monitor total bacterial communities and to establish a pattern of succession between species in vacuum-packaged beef stored at 2 and 8°C for 9 weeks and 14 days. Species-specific PCR was used to confirm the presence of Lactobacillus sakei and Lactobacillus curvatus. Multiplex PCRs using 16S rRNA-specific primers allowed differentiation between Leuconostoc species. These methods provided the desired information about microbial diversity by detecting the main microorganisms capable of colonizing this ecological niche. Copyright © 2006, American Society for Microbiology. All Rights Reserved.
publishDate 2006
dc.date.none.fl_str_mv 2006-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/55405
Fontana, Cecilia Alejandra; Cocconcelli, Pier Sandro; Vignolo, Graciela Margarita; Direct molecular approach to monitoring bacterial colonization on vacuum-packaged beef; American Society for Microbiology; Applied And Environmental Microbiology; 72; 8; 8-2006; 5618-5622
0099-2240
CONICET Digital
CONICET
url http://hdl.handle.net/11336/55405
identifier_str_mv Fontana, Cecilia Alejandra; Cocconcelli, Pier Sandro; Vignolo, Graciela Margarita; Direct molecular approach to monitoring bacterial colonization on vacuum-packaged beef; American Society for Microbiology; Applied And Environmental Microbiology; 72; 8; 8-2006; 5618-5622
0099-2240
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1128/AEM.00029-06
info:eu-repo/semantics/altIdentifier/url/http://aem.asm.org/content/72/8/5618
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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