Prevalence of Escherichia Coli O157:H7 in surface water near cattle feedlots

Autores
Tanaro, José Daniel; Piaggio, Mercedes Carolina; Galli, Lucía; Gasparovic, Alejandra M. C.; Procura, Francisco; Molina, Demián A.; Vitón, Mauro; Zolezzi, Gisella; Rivas, Marta
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Between April 2009 and July 2011, 311 surface water samples in 48 cattle feedlots distributed in an area of about 67,000 km2 were analyzed to examine the environmental dissemination of Escherichia coli O157:H7. Samples were taken inside and outside the pens, exposed and not exposed to runoff from corrals, near the feedlots. Two types of samples were defined: (1) exposed surface waters (ESW; n = 251), downstream from cattle pens; and (2) nonexposed surface waters (NESW; n = 60), upstream from cattle pens. By multiplex PCR, 177 (70.5%) ESW samples were rfbO157-positive, and 62 (24.7%) E. coli O157, and 32 (12.7%) Shiga toxin-producing E. coli (STEC) O157:H7 strains were isolated. In the NESW samples, 36 (60.0%) were rfbO157- positive, and 9 (15.0%) E. coli O157, and 6 (10.0%) STEC O157:H7 strains were isolated. These results showed that the environmental surface waters exposed to liquid discharges from intensive livestock operations tended to be contaminated with more STEC O157:H7 than NESW. However, no significant difference was found. This fact emphasizes the relevance of other horizontal routes of transmission, as the persistence of E. coli in the environment resulting from extensive livestock farming. By XbaIPFGE, some patterns identified are included in the Argentine Database of E. coli O157, corresponding to strains isolated from hemolytic uremic syndrome and diarrhea cases, food, and animals, such as AREXHX01.0022, second prevalent pattern in Argentina, representing 5.5% of the total database. In the study area, characterized by the abundance of waterways, pathogens contained in feedlot runoff could reach recreational waters and also contaminate produce through irrigation, increasing the potential dissemination of STEC O157:H7 and the risk of human infections. The control of runoff systems from intensive livestock is necessary, but other alternatives should be explored to solve the problem of the presence of E. coli O157 in the aquatic rural environment.
Fil: Tanaro, José Daniel. Universidad Nacional de Entre Rios. Facultad de Bromatologia; Argentina
Fil: Piaggio, Mercedes Carolina. Universidad Nacional de Entre Rios. Facultad de Bromatologia; Argentina
Fil: Galli, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Direccion Nacional de Instituto de Investigacion. Adm.nacional de Laboratorio E Instituto de Salud "dr.c.g.malbran". Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriologia; Argentina
Fil: Gasparovic, Alejandra M. C.. Universidad Nacional de Entre Rios. Facultad de Bromatologia; Argentina
Fil: Procura, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Entre Rios. Facultad de Bromatologia; Argentina
Fil: Molina, Demián A.. Universidad Nacional de Entre Rios. Facultad de Bromatologia; Argentina
Fil: Vitón, Mauro. Universidad Nacional de Entre Rios. Facultad de Bromatologia; Argentina
Fil: Zolezzi, Gisella. Direccion Nacional de Instituto de Investigacion. Adm.nacional de Laboratorio E Instituto de Salud "dr.c.g.malbran". Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriologia; Argentina
Fil: Rivas, Marta. Direccion Nacional de Instituto de Investigacion. Adm.nacional de Laboratorio E Instituto de Salud "dr.c.g.malbran". Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriologia; Argentina
Materia
Escherichia Coli O157
Cattle
Manure
Pre-Harvest Food Safety
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/11803
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Prevalence of Escherichia Coli O157:H7 in surface water near cattle feedlotsTanaro, José DanielPiaggio, Mercedes CarolinaGalli, LucíaGasparovic, Alejandra M. C.Procura, FranciscoMolina, Demián A.Vitón, Mauro Zolezzi, GisellaRivas, MartaEscherichia Coli O157CattleManurePre-Harvest Food Safetyhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Between April 2009 and July 2011, 311 surface water samples in 48 cattle feedlots distributed in an area of about 67,000 km2 were analyzed to examine the environmental dissemination of Escherichia coli O157:H7. Samples were taken inside and outside the pens, exposed and not exposed to runoff from corrals, near the feedlots. Two types of samples were defined: (1) exposed surface waters (ESW; n = 251), downstream from cattle pens; and (2) nonexposed surface waters (NESW; n = 60), upstream from cattle pens. By multiplex PCR, 177 (70.5%) ESW samples were rfbO157-positive, and 62 (24.7%) E. coli O157, and 32 (12.7%) Shiga toxin-producing E. coli (STEC) O157:H7 strains were isolated. In the NESW samples, 36 (60.0%) were rfbO157- positive, and 9 (15.0%) E. coli O157, and 6 (10.0%) STEC O157:H7 strains were isolated. These results showed that the environmental surface waters exposed to liquid discharges from intensive livestock operations tended to be contaminated with more STEC O157:H7 than NESW. However, no significant difference was found. This fact emphasizes the relevance of other horizontal routes of transmission, as the persistence of E. coli in the environment resulting from extensive livestock farming. By XbaIPFGE, some patterns identified are included in the Argentine Database of E. coli O157, corresponding to strains isolated from hemolytic uremic syndrome and diarrhea cases, food, and animals, such as AREXHX01.0022, second prevalent pattern in Argentina, representing 5.5% of the total database. In the study area, characterized by the abundance of waterways, pathogens contained in feedlot runoff could reach recreational waters and also contaminate produce through irrigation, increasing the potential dissemination of STEC O157:H7 and the risk of human infections. The control of runoff systems from intensive livestock is necessary, but other alternatives should be explored to solve the problem of the presence of E. coli O157 in the aquatic rural environment.Fil: Tanaro, José Daniel. Universidad Nacional de Entre Rios. Facultad de Bromatologia; ArgentinaFil: Piaggio, Mercedes Carolina. Universidad Nacional de Entre Rios. Facultad de Bromatologia; ArgentinaFil: Galli, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Direccion Nacional de Instituto de Investigacion. Adm.nacional de Laboratorio E Instituto de Salud "dr.c.g.malbran". Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriologia; ArgentinaFil: Gasparovic, Alejandra M. C.. Universidad Nacional de Entre Rios. Facultad de Bromatologia; ArgentinaFil: Procura, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Entre Rios. Facultad de Bromatologia; ArgentinaFil: Molina, Demián A.. Universidad Nacional de Entre Rios. Facultad de Bromatologia; ArgentinaFil: Vitón, Mauro. Universidad Nacional de Entre Rios. Facultad de Bromatologia; ArgentinaFil: Zolezzi, Gisella. Direccion Nacional de Instituto de Investigacion. Adm.nacional de Laboratorio E Instituto de Salud "dr.c.g.malbran". Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriologia; ArgentinaFil: Rivas, Marta. Direccion Nacional de Instituto de Investigacion. Adm.nacional de Laboratorio E Instituto de Salud "dr.c.g.malbran". Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriologia; ArgentinaMary Ann Liebert Inc2014-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/11803Tanaro, José Daniel; Piaggio, Mercedes Carolina; Galli, Lucía; Gasparovic, Alejandra M. C.; Procura, Francisco; et al.; Prevalence of Escherichia Coli O157:H7 in surface water near cattle feedlots; Mary Ann Liebert Inc; Foodborne Pathogens And Disease; 11; 12; 8-2014; 960-9651535-31411556-7125enginfo:eu-repo/semantics/altIdentifier/doi/10.1089/fpd.2014.1770info:eu-repo/semantics/altIdentifier/url/http://online.liebertpub.com/doi/abs/10.1089/fpd.2014.1770info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:46:41Zoai:ri.conicet.gov.ar:11336/11803instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:46:41.828CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Prevalence of Escherichia Coli O157:H7 in surface water near cattle feedlots
title Prevalence of Escherichia Coli O157:H7 in surface water near cattle feedlots
spellingShingle Prevalence of Escherichia Coli O157:H7 in surface water near cattle feedlots
Tanaro, José Daniel
Escherichia Coli O157
Cattle
Manure
Pre-Harvest Food Safety
title_short Prevalence of Escherichia Coli O157:H7 in surface water near cattle feedlots
title_full Prevalence of Escherichia Coli O157:H7 in surface water near cattle feedlots
title_fullStr Prevalence of Escherichia Coli O157:H7 in surface water near cattle feedlots
title_full_unstemmed Prevalence of Escherichia Coli O157:H7 in surface water near cattle feedlots
title_sort Prevalence of Escherichia Coli O157:H7 in surface water near cattle feedlots
dc.creator.none.fl_str_mv Tanaro, José Daniel
Piaggio, Mercedes Carolina
Galli, Lucía
Gasparovic, Alejandra M. C.
Procura, Francisco
Molina, Demián A.
Vitón, Mauro
Zolezzi, Gisella
Rivas, Marta
author Tanaro, José Daniel
author_facet Tanaro, José Daniel
Piaggio, Mercedes Carolina
Galli, Lucía
Gasparovic, Alejandra M. C.
Procura, Francisco
Molina, Demián A.
Vitón, Mauro
Zolezzi, Gisella
Rivas, Marta
author_role author
author2 Piaggio, Mercedes Carolina
Galli, Lucía
Gasparovic, Alejandra M. C.
Procura, Francisco
Molina, Demián A.
Vitón, Mauro
Zolezzi, Gisella
Rivas, Marta
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Escherichia Coli O157
Cattle
Manure
Pre-Harvest Food Safety
topic Escherichia Coli O157
Cattle
Manure
Pre-Harvest Food Safety
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Between April 2009 and July 2011, 311 surface water samples in 48 cattle feedlots distributed in an area of about 67,000 km2 were analyzed to examine the environmental dissemination of Escherichia coli O157:H7. Samples were taken inside and outside the pens, exposed and not exposed to runoff from corrals, near the feedlots. Two types of samples were defined: (1) exposed surface waters (ESW; n = 251), downstream from cattle pens; and (2) nonexposed surface waters (NESW; n = 60), upstream from cattle pens. By multiplex PCR, 177 (70.5%) ESW samples were rfbO157-positive, and 62 (24.7%) E. coli O157, and 32 (12.7%) Shiga toxin-producing E. coli (STEC) O157:H7 strains were isolated. In the NESW samples, 36 (60.0%) were rfbO157- positive, and 9 (15.0%) E. coli O157, and 6 (10.0%) STEC O157:H7 strains were isolated. These results showed that the environmental surface waters exposed to liquid discharges from intensive livestock operations tended to be contaminated with more STEC O157:H7 than NESW. However, no significant difference was found. This fact emphasizes the relevance of other horizontal routes of transmission, as the persistence of E. coli in the environment resulting from extensive livestock farming. By XbaIPFGE, some patterns identified are included in the Argentine Database of E. coli O157, corresponding to strains isolated from hemolytic uremic syndrome and diarrhea cases, food, and animals, such as AREXHX01.0022, second prevalent pattern in Argentina, representing 5.5% of the total database. In the study area, characterized by the abundance of waterways, pathogens contained in feedlot runoff could reach recreational waters and also contaminate produce through irrigation, increasing the potential dissemination of STEC O157:H7 and the risk of human infections. The control of runoff systems from intensive livestock is necessary, but other alternatives should be explored to solve the problem of the presence of E. coli O157 in the aquatic rural environment.
Fil: Tanaro, José Daniel. Universidad Nacional de Entre Rios. Facultad de Bromatologia; Argentina
Fil: Piaggio, Mercedes Carolina. Universidad Nacional de Entre Rios. Facultad de Bromatologia; Argentina
Fil: Galli, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Direccion Nacional de Instituto de Investigacion. Adm.nacional de Laboratorio E Instituto de Salud "dr.c.g.malbran". Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriologia; Argentina
Fil: Gasparovic, Alejandra M. C.. Universidad Nacional de Entre Rios. Facultad de Bromatologia; Argentina
Fil: Procura, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Entre Rios. Facultad de Bromatologia; Argentina
Fil: Molina, Demián A.. Universidad Nacional de Entre Rios. Facultad de Bromatologia; Argentina
Fil: Vitón, Mauro. Universidad Nacional de Entre Rios. Facultad de Bromatologia; Argentina
Fil: Zolezzi, Gisella. Direccion Nacional de Instituto de Investigacion. Adm.nacional de Laboratorio E Instituto de Salud "dr.c.g.malbran". Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriologia; Argentina
Fil: Rivas, Marta. Direccion Nacional de Instituto de Investigacion. Adm.nacional de Laboratorio E Instituto de Salud "dr.c.g.malbran". Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriologia; Argentina
description Between April 2009 and July 2011, 311 surface water samples in 48 cattle feedlots distributed in an area of about 67,000 km2 were analyzed to examine the environmental dissemination of Escherichia coli O157:H7. Samples were taken inside and outside the pens, exposed and not exposed to runoff from corrals, near the feedlots. Two types of samples were defined: (1) exposed surface waters (ESW; n = 251), downstream from cattle pens; and (2) nonexposed surface waters (NESW; n = 60), upstream from cattle pens. By multiplex PCR, 177 (70.5%) ESW samples were rfbO157-positive, and 62 (24.7%) E. coli O157, and 32 (12.7%) Shiga toxin-producing E. coli (STEC) O157:H7 strains were isolated. In the NESW samples, 36 (60.0%) were rfbO157- positive, and 9 (15.0%) E. coli O157, and 6 (10.0%) STEC O157:H7 strains were isolated. These results showed that the environmental surface waters exposed to liquid discharges from intensive livestock operations tended to be contaminated with more STEC O157:H7 than NESW. However, no significant difference was found. This fact emphasizes the relevance of other horizontal routes of transmission, as the persistence of E. coli in the environment resulting from extensive livestock farming. By XbaIPFGE, some patterns identified are included in the Argentine Database of E. coli O157, corresponding to strains isolated from hemolytic uremic syndrome and diarrhea cases, food, and animals, such as AREXHX01.0022, second prevalent pattern in Argentina, representing 5.5% of the total database. In the study area, characterized by the abundance of waterways, pathogens contained in feedlot runoff could reach recreational waters and also contaminate produce through irrigation, increasing the potential dissemination of STEC O157:H7 and the risk of human infections. The control of runoff systems from intensive livestock is necessary, but other alternatives should be explored to solve the problem of the presence of E. coli O157 in the aquatic rural environment.
publishDate 2014
dc.date.none.fl_str_mv 2014-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/11803
Tanaro, José Daniel; Piaggio, Mercedes Carolina; Galli, Lucía; Gasparovic, Alejandra M. C.; Procura, Francisco; et al.; Prevalence of Escherichia Coli O157:H7 in surface water near cattle feedlots; Mary Ann Liebert Inc; Foodborne Pathogens And Disease; 11; 12; 8-2014; 960-965
1535-3141
1556-7125
url http://hdl.handle.net/11336/11803
identifier_str_mv Tanaro, José Daniel; Piaggio, Mercedes Carolina; Galli, Lucía; Gasparovic, Alejandra M. C.; Procura, Francisco; et al.; Prevalence of Escherichia Coli O157:H7 in surface water near cattle feedlots; Mary Ann Liebert Inc; Foodborne Pathogens And Disease; 11; 12; 8-2014; 960-965
1535-3141
1556-7125
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1089/fpd.2014.1770
info:eu-repo/semantics/altIdentifier/url/http://online.liebertpub.com/doi/abs/10.1089/fpd.2014.1770
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Mary Ann Liebert Inc
publisher.none.fl_str_mv Mary Ann Liebert Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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