Floral regulators FLC and SOC1 directly regulate expression of the B3-type transcription factor TARGET OF FLC AND SVP 1 at the Arabidopsis shoot apex via antagonistic chromatin mod...

Autores
Richter, René; Kinoshita, Atsuko; Vincent, Coral; Martinez Gallegos, Rafael; Gao, He; van Driel, Annabel D.; Hyun, Youbong; Mateos, Julieta Lisa; Coupland, George
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Integration of environmental and endogenous cues at plant shoot meristems determines the timing of flowering and reproductive development. The MADS box transcription factor FLOWERING LOCUS C (FLC) of Arabidopsis thaliana is an important repressor of floral transition, which blocks flowering until plants are exposed to winter cold. However, the target genes of FLC have not been thoroughly described, and our understanding of the mechanisms by which FLC represses transcription of these targets and how this repression is overcome during floral transition is still fragmentary. Here, we identify and characterize TARGET OF FLC AND SVP1 (TFS1), a novel target gene of FLC and its interacting protein SHORT VEGETATIVE PHASE (SVP). TFS1 encodes a B3-type transcription factor, and we show that tfs1 mutants are later flowering than wild-type, particularly under short days. FLC and SVP repress TFS1 transcription leading to deposition of trimethylation of Iysine 27 of histone 3 (H3K27me3) by the Polycomb Repressive Complex 2 at the TFS1 locus. During floral transition, after downregulation of FLC by cold, TFS1 transcription is promoted by SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1), a MADS box protein encoded by another target of FLC/SVP. SOC1 opposes PRC function at TFS1 through recruitment of the histone demethylase RELATIVE OF EARLY FLOWERING 6 (REF6) and the SWI/SNF chromatin remodeler ATPase BRAHMA (BRM). This recruitment of BRM is also strictly required for SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 9 (SPL9) binding at TFS1 to coordinate RNAPII recruitment through the Mediator complex. Thus, we show that antagonistic chromatin modifications mediated by different MADS box transcription factor complexes play a crucial role in defining the temporal and spatial patterns of transcription of genes within a network of interactions downstream of FLC/SVP during floral transition.
Fil: Richter, René. Max Planck Institute for Plant Breeding. Department of Plant Developmental Biology; Alemania
Fil: Kinoshita, Atsuko. Max Planck Institute for Plant Breeding. Department of Plant Developmental Biology; Alemania
Fil: Vincent, Coral. Max Planck Institute for Plant Breeding. Department of Plant Developmental Biology; Alemania
Fil: Martinez Gallegos, Rafael. Max Planck Institute for Plant Breeding. Department of Plant Developmental Biology; Alemania
Fil: Gao, He. Max Planck Institute for Plant Breeding. Department of Plant Developmental Biology; Alemania
Fil: van Driel, Annabel D.. Max Planck Institute for Plant Breeding. Department of Plant Developmental Biology; Alemania
Fil: Hyun, Youbong. Max Planck Institute for Plant Breeding. Department of Plant Developmental Biology; Alemania
Fil: Mateos, Julieta Lisa. Max Planck Institute for Plant Breeding. Department of Plant Developmental Biology; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Coupland, George. Max Planck Institute for Plant Breeding. Department of Plant Developmental Biology; Alemania
Materia
FLOWERING
FLC
SVP
TRANSCRIPTION FACTORS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/105029
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Floral regulators FLC and SOC1 directly regulate expression of the B3-type transcription factor TARGET OF FLC AND SVP 1 at the Arabidopsis shoot apex via antagonistic chromatin modificationsRichter, RenéKinoshita, AtsukoVincent, CoralMartinez Gallegos, RafaelGao, Hevan Driel, Annabel D.Hyun, YoubongMateos, Julieta LisaCoupland, GeorgeFLOWERINGFLCSVPTRANSCRIPTION FACTORShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Integration of environmental and endogenous cues at plant shoot meristems determines the timing of flowering and reproductive development. The MADS box transcription factor FLOWERING LOCUS C (FLC) of Arabidopsis thaliana is an important repressor of floral transition, which blocks flowering until plants are exposed to winter cold. However, the target genes of FLC have not been thoroughly described, and our understanding of the mechanisms by which FLC represses transcription of these targets and how this repression is overcome during floral transition is still fragmentary. Here, we identify and characterize TARGET OF FLC AND SVP1 (TFS1), a novel target gene of FLC and its interacting protein SHORT VEGETATIVE PHASE (SVP). TFS1 encodes a B3-type transcription factor, and we show that tfs1 mutants are later flowering than wild-type, particularly under short days. FLC and SVP repress TFS1 transcription leading to deposition of trimethylation of Iysine 27 of histone 3 (H3K27me3) by the Polycomb Repressive Complex 2 at the TFS1 locus. During floral transition, after downregulation of FLC by cold, TFS1 transcription is promoted by SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1), a MADS box protein encoded by another target of FLC/SVP. SOC1 opposes PRC function at TFS1 through recruitment of the histone demethylase RELATIVE OF EARLY FLOWERING 6 (REF6) and the SWI/SNF chromatin remodeler ATPase BRAHMA (BRM). This recruitment of BRM is also strictly required for SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 9 (SPL9) binding at TFS1 to coordinate RNAPII recruitment through the Mediator complex. Thus, we show that antagonistic chromatin modifications mediated by different MADS box transcription factor complexes play a crucial role in defining the temporal and spatial patterns of transcription of genes within a network of interactions downstream of FLC/SVP during floral transition.Fil: Richter, René. Max Planck Institute for Plant Breeding. Department of Plant Developmental Biology; AlemaniaFil: Kinoshita, Atsuko. Max Planck Institute for Plant Breeding. Department of Plant Developmental Biology; AlemaniaFil: Vincent, Coral. Max Planck Institute for Plant Breeding. Department of Plant Developmental Biology; AlemaniaFil: Martinez Gallegos, Rafael. Max Planck Institute for Plant Breeding. Department of Plant Developmental Biology; AlemaniaFil: Gao, He. Max Planck Institute for Plant Breeding. Department of Plant Developmental Biology; AlemaniaFil: van Driel, Annabel D.. Max Planck Institute for Plant Breeding. Department of Plant Developmental Biology; AlemaniaFil: Hyun, Youbong. Max Planck Institute for Plant Breeding. Department of Plant Developmental Biology; AlemaniaFil: Mateos, Julieta Lisa. Max Planck Institute for Plant Breeding. Department of Plant Developmental Biology; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Coupland, George. Max Planck Institute for Plant Breeding. Department of Plant Developmental Biology; AlemaniaPublic Library of Science2019-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/105029Richter, René; Kinoshita, Atsuko; Vincent, Coral; Martinez Gallegos, Rafael; Gao, He; et al.; Floral regulators FLC and SOC1 directly regulate expression of the B3-type transcription factor TARGET OF FLC AND SVP 1 at the Arabidopsis shoot apex via antagonistic chromatin modifications; Public Library of Science; Plos Genetics; 15; 4; 4-2019; 1-27; e10080651553-73901553-7404CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1008065info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pgen.1008065info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:20:47Zoai:ri.conicet.gov.ar:11336/105029instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:20:47.657CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Floral regulators FLC and SOC1 directly regulate expression of the B3-type transcription factor TARGET OF FLC AND SVP 1 at the Arabidopsis shoot apex via antagonistic chromatin modifications
title Floral regulators FLC and SOC1 directly regulate expression of the B3-type transcription factor TARGET OF FLC AND SVP 1 at the Arabidopsis shoot apex via antagonistic chromatin modifications
spellingShingle Floral regulators FLC and SOC1 directly regulate expression of the B3-type transcription factor TARGET OF FLC AND SVP 1 at the Arabidopsis shoot apex via antagonistic chromatin modifications
Richter, René
FLOWERING
FLC
SVP
TRANSCRIPTION FACTORS
title_short Floral regulators FLC and SOC1 directly regulate expression of the B3-type transcription factor TARGET OF FLC AND SVP 1 at the Arabidopsis shoot apex via antagonistic chromatin modifications
title_full Floral regulators FLC and SOC1 directly regulate expression of the B3-type transcription factor TARGET OF FLC AND SVP 1 at the Arabidopsis shoot apex via antagonistic chromatin modifications
title_fullStr Floral regulators FLC and SOC1 directly regulate expression of the B3-type transcription factor TARGET OF FLC AND SVP 1 at the Arabidopsis shoot apex via antagonistic chromatin modifications
title_full_unstemmed Floral regulators FLC and SOC1 directly regulate expression of the B3-type transcription factor TARGET OF FLC AND SVP 1 at the Arabidopsis shoot apex via antagonistic chromatin modifications
title_sort Floral regulators FLC and SOC1 directly regulate expression of the B3-type transcription factor TARGET OF FLC AND SVP 1 at the Arabidopsis shoot apex via antagonistic chromatin modifications
dc.creator.none.fl_str_mv Richter, René
Kinoshita, Atsuko
Vincent, Coral
Martinez Gallegos, Rafael
Gao, He
van Driel, Annabel D.
Hyun, Youbong
Mateos, Julieta Lisa
Coupland, George
author Richter, René
author_facet Richter, René
Kinoshita, Atsuko
Vincent, Coral
Martinez Gallegos, Rafael
Gao, He
van Driel, Annabel D.
Hyun, Youbong
Mateos, Julieta Lisa
Coupland, George
author_role author
author2 Kinoshita, Atsuko
Vincent, Coral
Martinez Gallegos, Rafael
Gao, He
van Driel, Annabel D.
Hyun, Youbong
Mateos, Julieta Lisa
Coupland, George
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv FLOWERING
FLC
SVP
TRANSCRIPTION FACTORS
topic FLOWERING
FLC
SVP
TRANSCRIPTION FACTORS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Integration of environmental and endogenous cues at plant shoot meristems determines the timing of flowering and reproductive development. The MADS box transcription factor FLOWERING LOCUS C (FLC) of Arabidopsis thaliana is an important repressor of floral transition, which blocks flowering until plants are exposed to winter cold. However, the target genes of FLC have not been thoroughly described, and our understanding of the mechanisms by which FLC represses transcription of these targets and how this repression is overcome during floral transition is still fragmentary. Here, we identify and characterize TARGET OF FLC AND SVP1 (TFS1), a novel target gene of FLC and its interacting protein SHORT VEGETATIVE PHASE (SVP). TFS1 encodes a B3-type transcription factor, and we show that tfs1 mutants are later flowering than wild-type, particularly under short days. FLC and SVP repress TFS1 transcription leading to deposition of trimethylation of Iysine 27 of histone 3 (H3K27me3) by the Polycomb Repressive Complex 2 at the TFS1 locus. During floral transition, after downregulation of FLC by cold, TFS1 transcription is promoted by SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1), a MADS box protein encoded by another target of FLC/SVP. SOC1 opposes PRC function at TFS1 through recruitment of the histone demethylase RELATIVE OF EARLY FLOWERING 6 (REF6) and the SWI/SNF chromatin remodeler ATPase BRAHMA (BRM). This recruitment of BRM is also strictly required for SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 9 (SPL9) binding at TFS1 to coordinate RNAPII recruitment through the Mediator complex. Thus, we show that antagonistic chromatin modifications mediated by different MADS box transcription factor complexes play a crucial role in defining the temporal and spatial patterns of transcription of genes within a network of interactions downstream of FLC/SVP during floral transition.
Fil: Richter, René. Max Planck Institute for Plant Breeding. Department of Plant Developmental Biology; Alemania
Fil: Kinoshita, Atsuko. Max Planck Institute for Plant Breeding. Department of Plant Developmental Biology; Alemania
Fil: Vincent, Coral. Max Planck Institute for Plant Breeding. Department of Plant Developmental Biology; Alemania
Fil: Martinez Gallegos, Rafael. Max Planck Institute for Plant Breeding. Department of Plant Developmental Biology; Alemania
Fil: Gao, He. Max Planck Institute for Plant Breeding. Department of Plant Developmental Biology; Alemania
Fil: van Driel, Annabel D.. Max Planck Institute for Plant Breeding. Department of Plant Developmental Biology; Alemania
Fil: Hyun, Youbong. Max Planck Institute for Plant Breeding. Department of Plant Developmental Biology; Alemania
Fil: Mateos, Julieta Lisa. Max Planck Institute for Plant Breeding. Department of Plant Developmental Biology; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Coupland, George. Max Planck Institute for Plant Breeding. Department of Plant Developmental Biology; Alemania
description Integration of environmental and endogenous cues at plant shoot meristems determines the timing of flowering and reproductive development. The MADS box transcription factor FLOWERING LOCUS C (FLC) of Arabidopsis thaliana is an important repressor of floral transition, which blocks flowering until plants are exposed to winter cold. However, the target genes of FLC have not been thoroughly described, and our understanding of the mechanisms by which FLC represses transcription of these targets and how this repression is overcome during floral transition is still fragmentary. Here, we identify and characterize TARGET OF FLC AND SVP1 (TFS1), a novel target gene of FLC and its interacting protein SHORT VEGETATIVE PHASE (SVP). TFS1 encodes a B3-type transcription factor, and we show that tfs1 mutants are later flowering than wild-type, particularly under short days. FLC and SVP repress TFS1 transcription leading to deposition of trimethylation of Iysine 27 of histone 3 (H3K27me3) by the Polycomb Repressive Complex 2 at the TFS1 locus. During floral transition, after downregulation of FLC by cold, TFS1 transcription is promoted by SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1), a MADS box protein encoded by another target of FLC/SVP. SOC1 opposes PRC function at TFS1 through recruitment of the histone demethylase RELATIVE OF EARLY FLOWERING 6 (REF6) and the SWI/SNF chromatin remodeler ATPase BRAHMA (BRM). This recruitment of BRM is also strictly required for SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 9 (SPL9) binding at TFS1 to coordinate RNAPII recruitment through the Mediator complex. Thus, we show that antagonistic chromatin modifications mediated by different MADS box transcription factor complexes play a crucial role in defining the temporal and spatial patterns of transcription of genes within a network of interactions downstream of FLC/SVP during floral transition.
publishDate 2019
dc.date.none.fl_str_mv 2019-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/105029
Richter, René; Kinoshita, Atsuko; Vincent, Coral; Martinez Gallegos, Rafael; Gao, He; et al.; Floral regulators FLC and SOC1 directly regulate expression of the B3-type transcription factor TARGET OF FLC AND SVP 1 at the Arabidopsis shoot apex via antagonistic chromatin modifications; Public Library of Science; Plos Genetics; 15; 4; 4-2019; 1-27; e1008065
1553-7390
1553-7404
CONICET Digital
CONICET
url http://hdl.handle.net/11336/105029
identifier_str_mv Richter, René; Kinoshita, Atsuko; Vincent, Coral; Martinez Gallegos, Rafael; Gao, He; et al.; Floral regulators FLC and SOC1 directly regulate expression of the B3-type transcription factor TARGET OF FLC AND SVP 1 at the Arabidopsis shoot apex via antagonistic chromatin modifications; Public Library of Science; Plos Genetics; 15; 4; 4-2019; 1-27; e1008065
1553-7390
1553-7404
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1008065
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pgen.1008065
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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