Divergence of regulatory networks governed by the orthologous transcription factors FLC and PEP1 in Brassicaceae species

Autores
Mateos, Julieta Lisa; Tilmes, Vicky; Madrigal, Pedro; Severing, Edouard; Richter, René; Rijkenberg, Colin W.M.; Krajewski, Pawel; Coupland, George
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Genome-wide landscapes of transcription factor (TF) binding sites (BSs) diverge during evolution, conferring species-specific transcriptional patterns. The rate of divergence varies in different metazoan lineages but has not been widely studied in plants. We identified the BSs and assessed the effects on transcription of FLOWERING LOCUS C (FLC) and PERPETUAL FLOWERING 1 (PEP1), two orthologous MADS-box TFs that repress flowering and confer vernalization requirement in the Brassicaceae species Arabidopsis thaliana and Arabis alpina, respectively. We found that only 14% of their BSs were conserved in both species and that these contained a CArG-box that is recognized by MADS-box TFs. The CArG-box consensus at conserved BSs was extended compared with the core motif. By contrast, species-specific BSs usually lacked the CArG-box in the other species. Flowering-time genes were highly over-represented among conserved targets, and their CArG-boxes were widely conserved among Brassicaceae species. Cold-regulated (COR) genes were also overrepresented among targets, but the cognate BSs and the identity of the regulated genes were usually different in each species. In cold, COR gene transcript levels were increased in flc and pep1-1 mutants compared with WT, and this correlated with reduced growth in pep1-1. Therefore, FLC orthologs regulate a set of conserved target genes mainly involved in reproductive development and were later independently recruited to modulate stress responses in different Brassicaceae lineages. Analysis of TF BSs in these lineages thus distinguishes widely conserved targets representing the core function of the TF from those that were recruited later in evolution.
Fil: Mateos, Julieta Lisa. Max Planck Institute Fur Zuchtungsforschung; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Tilmes, Vicky. Max Planck Institute Fur Zuchtungsforschung; Alemania
Fil: Madrigal, Pedro. Polish Academy of Sciences; Argentina
Fil: Severing, Edouard. Max Planck Institute Fur Zuchtungsforschung; Alemania
Fil: Richter, René. Max Planck Institute Fur Zuchtungsforschung; Alemania
Fil: Rijkenberg, Colin W.M.. Max Planck Institute Fur Zuchtungsforschung; Alemania
Fil: Krajewski, Pawel. Polish Academy of Sciences; Argentina
Fil: Coupland, George. Max Planck Institute Fur Zuchtungsforschung; Alemania
Materia
ARABIDOPSIS
ARABIS ALPINA
BINDING-SITE EVOLUTION
FLOWERING
VERNALIZATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/40863
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/40863
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Divergence of regulatory networks governed by the orthologous transcription factors FLC and PEP1 in Brassicaceae speciesMateos, Julieta LisaTilmes, VickyMadrigal, PedroSevering, EdouardRichter, RenéRijkenberg, Colin W.M.Krajewski, PawelCoupland, GeorgeARABIDOPSISARABIS ALPINABINDING-SITE EVOLUTIONFLOWERINGVERNALIZATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Genome-wide landscapes of transcription factor (TF) binding sites (BSs) diverge during evolution, conferring species-specific transcriptional patterns. The rate of divergence varies in different metazoan lineages but has not been widely studied in plants. We identified the BSs and assessed the effects on transcription of FLOWERING LOCUS C (FLC) and PERPETUAL FLOWERING 1 (PEP1), two orthologous MADS-box TFs that repress flowering and confer vernalization requirement in the Brassicaceae species Arabidopsis thaliana and Arabis alpina, respectively. We found that only 14% of their BSs were conserved in both species and that these contained a CArG-box that is recognized by MADS-box TFs. The CArG-box consensus at conserved BSs was extended compared with the core motif. By contrast, species-specific BSs usually lacked the CArG-box in the other species. Flowering-time genes were highly over-represented among conserved targets, and their CArG-boxes were widely conserved among Brassicaceae species. Cold-regulated (COR) genes were also overrepresented among targets, but the cognate BSs and the identity of the regulated genes were usually different in each species. In cold, COR gene transcript levels were increased in flc and pep1-1 mutants compared with WT, and this correlated with reduced growth in pep1-1. Therefore, FLC orthologs regulate a set of conserved target genes mainly involved in reproductive development and were later independently recruited to modulate stress responses in different Brassicaceae lineages. Analysis of TF BSs in these lineages thus distinguishes widely conserved targets representing the core function of the TF from those that were recruited later in evolution.Fil: Mateos, Julieta Lisa. Max Planck Institute Fur Zuchtungsforschung; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Tilmes, Vicky. Max Planck Institute Fur Zuchtungsforschung; AlemaniaFil: Madrigal, Pedro. Polish Academy of Sciences; ArgentinaFil: Severing, Edouard. Max Planck Institute Fur Zuchtungsforschung; AlemaniaFil: Richter, René. Max Planck Institute Fur Zuchtungsforschung; AlemaniaFil: Rijkenberg, Colin W.M.. Max Planck Institute Fur Zuchtungsforschung; AlemaniaFil: Krajewski, Pawel. Polish Academy of Sciences; ArgentinaFil: Coupland, George. Max Planck Institute Fur Zuchtungsforschung; AlemaniaNational Academy of Sciences2017-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/40863Mateos, Julieta Lisa; Tilmes, Vicky; Madrigal, Pedro; Severing, Edouard; Richter, René; et al.; Divergence of regulatory networks governed by the orthologous transcription factors FLC and PEP1 in Brassicaceae species; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 114; 51; 12-2017; E11037-E110460027-84241091-6490CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1073/pnas.1618075114info:eu-repo/semantics/altIdentifier/url/http://www.pnas.org/content/114/51/E11037.longinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:03:38Zoai:ri.conicet.gov.ar:11336/40863instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:03:39.079CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Divergence of regulatory networks governed by the orthologous transcription factors FLC and PEP1 in Brassicaceae species
title Divergence of regulatory networks governed by the orthologous transcription factors FLC and PEP1 in Brassicaceae species
spellingShingle Divergence of regulatory networks governed by the orthologous transcription factors FLC and PEP1 in Brassicaceae species
Mateos, Julieta Lisa
ARABIDOPSIS
ARABIS ALPINA
BINDING-SITE EVOLUTION
FLOWERING
VERNALIZATION
title_short Divergence of regulatory networks governed by the orthologous transcription factors FLC and PEP1 in Brassicaceae species
title_full Divergence of regulatory networks governed by the orthologous transcription factors FLC and PEP1 in Brassicaceae species
title_fullStr Divergence of regulatory networks governed by the orthologous transcription factors FLC and PEP1 in Brassicaceae species
title_full_unstemmed Divergence of regulatory networks governed by the orthologous transcription factors FLC and PEP1 in Brassicaceae species
title_sort Divergence of regulatory networks governed by the orthologous transcription factors FLC and PEP1 in Brassicaceae species
dc.creator.none.fl_str_mv Mateos, Julieta Lisa
Tilmes, Vicky
Madrigal, Pedro
Severing, Edouard
Richter, René
Rijkenberg, Colin W.M.
Krajewski, Pawel
Coupland, George
author Mateos, Julieta Lisa
author_facet Mateos, Julieta Lisa
Tilmes, Vicky
Madrigal, Pedro
Severing, Edouard
Richter, René
Rijkenberg, Colin W.M.
Krajewski, Pawel
Coupland, George
author_role author
author2 Tilmes, Vicky
Madrigal, Pedro
Severing, Edouard
Richter, René
Rijkenberg, Colin W.M.
Krajewski, Pawel
Coupland, George
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ARABIDOPSIS
ARABIS ALPINA
BINDING-SITE EVOLUTION
FLOWERING
VERNALIZATION
topic ARABIDOPSIS
ARABIS ALPINA
BINDING-SITE EVOLUTION
FLOWERING
VERNALIZATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Genome-wide landscapes of transcription factor (TF) binding sites (BSs) diverge during evolution, conferring species-specific transcriptional patterns. The rate of divergence varies in different metazoan lineages but has not been widely studied in plants. We identified the BSs and assessed the effects on transcription of FLOWERING LOCUS C (FLC) and PERPETUAL FLOWERING 1 (PEP1), two orthologous MADS-box TFs that repress flowering and confer vernalization requirement in the Brassicaceae species Arabidopsis thaliana and Arabis alpina, respectively. We found that only 14% of their BSs were conserved in both species and that these contained a CArG-box that is recognized by MADS-box TFs. The CArG-box consensus at conserved BSs was extended compared with the core motif. By contrast, species-specific BSs usually lacked the CArG-box in the other species. Flowering-time genes were highly over-represented among conserved targets, and their CArG-boxes were widely conserved among Brassicaceae species. Cold-regulated (COR) genes were also overrepresented among targets, but the cognate BSs and the identity of the regulated genes were usually different in each species. In cold, COR gene transcript levels were increased in flc and pep1-1 mutants compared with WT, and this correlated with reduced growth in pep1-1. Therefore, FLC orthologs regulate a set of conserved target genes mainly involved in reproductive development and were later independently recruited to modulate stress responses in different Brassicaceae lineages. Analysis of TF BSs in these lineages thus distinguishes widely conserved targets representing the core function of the TF from those that were recruited later in evolution.
Fil: Mateos, Julieta Lisa. Max Planck Institute Fur Zuchtungsforschung; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Tilmes, Vicky. Max Planck Institute Fur Zuchtungsforschung; Alemania
Fil: Madrigal, Pedro. Polish Academy of Sciences; Argentina
Fil: Severing, Edouard. Max Planck Institute Fur Zuchtungsforschung; Alemania
Fil: Richter, René. Max Planck Institute Fur Zuchtungsforschung; Alemania
Fil: Rijkenberg, Colin W.M.. Max Planck Institute Fur Zuchtungsforschung; Alemania
Fil: Krajewski, Pawel. Polish Academy of Sciences; Argentina
Fil: Coupland, George. Max Planck Institute Fur Zuchtungsforschung; Alemania
description Genome-wide landscapes of transcription factor (TF) binding sites (BSs) diverge during evolution, conferring species-specific transcriptional patterns. The rate of divergence varies in different metazoan lineages but has not been widely studied in plants. We identified the BSs and assessed the effects on transcription of FLOWERING LOCUS C (FLC) and PERPETUAL FLOWERING 1 (PEP1), two orthologous MADS-box TFs that repress flowering and confer vernalization requirement in the Brassicaceae species Arabidopsis thaliana and Arabis alpina, respectively. We found that only 14% of their BSs were conserved in both species and that these contained a CArG-box that is recognized by MADS-box TFs. The CArG-box consensus at conserved BSs was extended compared with the core motif. By contrast, species-specific BSs usually lacked the CArG-box in the other species. Flowering-time genes were highly over-represented among conserved targets, and their CArG-boxes were widely conserved among Brassicaceae species. Cold-regulated (COR) genes were also overrepresented among targets, but the cognate BSs and the identity of the regulated genes were usually different in each species. In cold, COR gene transcript levels were increased in flc and pep1-1 mutants compared with WT, and this correlated with reduced growth in pep1-1. Therefore, FLC orthologs regulate a set of conserved target genes mainly involved in reproductive development and were later independently recruited to modulate stress responses in different Brassicaceae lineages. Analysis of TF BSs in these lineages thus distinguishes widely conserved targets representing the core function of the TF from those that were recruited later in evolution.
publishDate 2017
dc.date.none.fl_str_mv 2017-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/40863
Mateos, Julieta Lisa; Tilmes, Vicky; Madrigal, Pedro; Severing, Edouard; Richter, René; et al.; Divergence of regulatory networks governed by the orthologous transcription factors FLC and PEP1 in Brassicaceae species; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 114; 51; 12-2017; E11037-E11046
0027-8424
1091-6490
CONICET Digital
CONICET
url http://hdl.handle.net/11336/40863
identifier_str_mv Mateos, Julieta Lisa; Tilmes, Vicky; Madrigal, Pedro; Severing, Edouard; Richter, René; et al.; Divergence of regulatory networks governed by the orthologous transcription factors FLC and PEP1 in Brassicaceae species; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 114; 51; 12-2017; E11037-E11046
0027-8424
1091-6490
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1073/pnas.1618075114
info:eu-repo/semantics/altIdentifier/url/http://www.pnas.org/content/114/51/E11037.long
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv National Academy of Sciences
publisher.none.fl_str_mv National Academy of Sciences
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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