Short vegetative phase reduces gibberellin biosynthesis at the arabidopsis shoot apex to regulate the floral transition

Autores
Andrés, Fernando; Porri, Aimone; Torti, Stefano; Mateos, Julieta Lisa; Romera Branchat, Maida; García Martínez, José Luis; Fornara, Fabio; Gregis, Veronica; Kater, Martin M.; Coupland, George
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In Arabidopsis thaliana environmental and endogenous cues promote flowering by activating expression of a small number of integrator genes. The MADS box transcription factor SHORT VEGETATIVE PHASE (SVP) is a critical inhibitor of flowering that directly represses transcription of these genes. However, we show by genetic analysis that the effect of SVP cannot be fully explained by repressing known floral integrator genes. To identify additional SVP functions, we analyzed genome-wide transcriptome data and show that GIBBERELLIN 20 OXIDASE 2, which encodes an enzyme required for biosynthesis of the growth regulator gibberellin (GA), is upregulated in svp mutants. GA is known to promote flowering, and we find that svp mutants contain elevated levels of GA that correlate with GA-related phenotypes such as early flowering and organ elongation. The ga20ox2 mutation suppresses the elevated GA levels and partially suppresses the growth and early flowering phenotypes of svp mutants. In wild-type plants, SVP expression in the shoot apical meristem falls when plants are exposed to photoperiods that induce flowering, and this correlates with increased expression of GA20ox2. Mutations that impair the photoperiodic flowering pathway prevent this downregulation of SVP and the strong increase in expression of GA20ox2. We conclude that SVP delays flowering by repressing GA biosynthesis as well as integrator gene expression and that, in response to inductive photoperiods, repression of SVP contributes to the rise in GA at the shoot apex, promoting rapid induction of flowering.
Fil: Andrés, Fernando. Max Planck Institute for Plant Breeding Research; Alemania
Fil: Porri, Aimone. Max Planck Institute for Plant Breeding Research; Alemania
Fil: Torti, Stefano. Max Planck Institute for Plant Breeding Research; Alemania
Fil: Mateos, Julieta Lisa. Max Planck Institute for Plant Breeding Research; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Fundación Instituto Leloir; Argentina
Fil: Romera Branchat, Maida. Max Planck Institute for Plant Breeding Research; Alemania
Fil: García Martínez, José Luis. Universidad Politécnica de Valencia. Instituto de Biología Molecular y Celular de Plantas; España
Fil: Fornara, Fabio. Università degli Studi di Milano. Department of Bioscience; Italia. Max Planck Institute for Plant Breeding Research; Alemania
Fil: Gregis, Veronica. Università degli Studi di Milano. Department of Bioscience; Italia
Fil: Kater, Martin M.. Università degli Studi di Milano. Department of Bioscience; Italia
Fil: Coupland, George. Max Planck Institute for Plant Breeding Research; Alemania
Materia
Short Vegetative Phase
Gibberellin
Arabidopsis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/8347
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/8347
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Short vegetative phase reduces gibberellin biosynthesis at the arabidopsis shoot apex to regulate the floral transitionAndrés, FernandoPorri, AimoneTorti, StefanoMateos, Julieta LisaRomera Branchat, MaidaGarcía Martínez, José LuisFornara, FabioGregis, VeronicaKater, Martin M.Coupland, GeorgeShort Vegetative PhaseGibberellinArabidopsishttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In Arabidopsis thaliana environmental and endogenous cues promote flowering by activating expression of a small number of integrator genes. The MADS box transcription factor SHORT VEGETATIVE PHASE (SVP) is a critical inhibitor of flowering that directly represses transcription of these genes. However, we show by genetic analysis that the effect of SVP cannot be fully explained by repressing known floral integrator genes. To identify additional SVP functions, we analyzed genome-wide transcriptome data and show that GIBBERELLIN 20 OXIDASE 2, which encodes an enzyme required for biosynthesis of the growth regulator gibberellin (GA), is upregulated in svp mutants. GA is known to promote flowering, and we find that svp mutants contain elevated levels of GA that correlate with GA-related phenotypes such as early flowering and organ elongation. The ga20ox2 mutation suppresses the elevated GA levels and partially suppresses the growth and early flowering phenotypes of svp mutants. In wild-type plants, SVP expression in the shoot apical meristem falls when plants are exposed to photoperiods that induce flowering, and this correlates with increased expression of GA20ox2. Mutations that impair the photoperiodic flowering pathway prevent this downregulation of SVP and the strong increase in expression of GA20ox2. We conclude that SVP delays flowering by repressing GA biosynthesis as well as integrator gene expression and that, in response to inductive photoperiods, repression of SVP contributes to the rise in GA at the shoot apex, promoting rapid induction of flowering.Fil: Andrés, Fernando. Max Planck Institute for Plant Breeding Research; AlemaniaFil: Porri, Aimone. Max Planck Institute for Plant Breeding Research; AlemaniaFil: Torti, Stefano. Max Planck Institute for Plant Breeding Research; AlemaniaFil: Mateos, Julieta Lisa. Max Planck Institute for Plant Breeding Research; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Fundación Instituto Leloir; ArgentinaFil: Romera Branchat, Maida. Max Planck Institute for Plant Breeding Research; AlemaniaFil: García Martínez, José Luis. Universidad Politécnica de Valencia. Instituto de Biología Molecular y Celular de Plantas; EspañaFil: Fornara, Fabio. Università degli Studi di Milano. Department of Bioscience; Italia. Max Planck Institute for Plant Breeding Research; AlemaniaFil: Gregis, Veronica. Università degli Studi di Milano. Department of Bioscience; ItaliaFil: Kater, Martin M.. Università degli Studi di Milano. Department of Bioscience; ItaliaFil: Coupland, George. Max Planck Institute for Plant Breeding Research; AlemaniaNational Academy Of Sciences2014-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/8347Andrés, Fernando; Porri, Aimone; Torti, Stefano; Mateos, Julieta Lisa; Romera Branchat, Maida; et al.; Short vegetative phase reduces gibberellin biosynthesis at the arabidopsis shoot apex to regulate the floral transition; National Academy Of Sciences; Proceedings Of The National Academy Of Sciences Of The United States Of America; 111; 26; 6-2014; E2760-E27690027-8424enginfo:eu-repo/semantics/altIdentifier/url/http://www.pnas.org/content/111/26/E2760info:eu-repo/semantics/altIdentifier/doi/10.1073/pnas.1409567111info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:55:13Zoai:ri.conicet.gov.ar:11336/8347instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:55:13.792CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Short vegetative phase reduces gibberellin biosynthesis at the arabidopsis shoot apex to regulate the floral transition
title Short vegetative phase reduces gibberellin biosynthesis at the arabidopsis shoot apex to regulate the floral transition
spellingShingle Short vegetative phase reduces gibberellin biosynthesis at the arabidopsis shoot apex to regulate the floral transition
Andrés, Fernando
Short Vegetative Phase
Gibberellin
Arabidopsis
title_short Short vegetative phase reduces gibberellin biosynthesis at the arabidopsis shoot apex to regulate the floral transition
title_full Short vegetative phase reduces gibberellin biosynthesis at the arabidopsis shoot apex to regulate the floral transition
title_fullStr Short vegetative phase reduces gibberellin biosynthesis at the arabidopsis shoot apex to regulate the floral transition
title_full_unstemmed Short vegetative phase reduces gibberellin biosynthesis at the arabidopsis shoot apex to regulate the floral transition
title_sort Short vegetative phase reduces gibberellin biosynthesis at the arabidopsis shoot apex to regulate the floral transition
dc.creator.none.fl_str_mv Andrés, Fernando
Porri, Aimone
Torti, Stefano
Mateos, Julieta Lisa
Romera Branchat, Maida
García Martínez, José Luis
Fornara, Fabio
Gregis, Veronica
Kater, Martin M.
Coupland, George
author Andrés, Fernando
author_facet Andrés, Fernando
Porri, Aimone
Torti, Stefano
Mateos, Julieta Lisa
Romera Branchat, Maida
García Martínez, José Luis
Fornara, Fabio
Gregis, Veronica
Kater, Martin M.
Coupland, George
author_role author
author2 Porri, Aimone
Torti, Stefano
Mateos, Julieta Lisa
Romera Branchat, Maida
García Martínez, José Luis
Fornara, Fabio
Gregis, Veronica
Kater, Martin M.
Coupland, George
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Short Vegetative Phase
Gibberellin
Arabidopsis
topic Short Vegetative Phase
Gibberellin
Arabidopsis
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In Arabidopsis thaliana environmental and endogenous cues promote flowering by activating expression of a small number of integrator genes. The MADS box transcription factor SHORT VEGETATIVE PHASE (SVP) is a critical inhibitor of flowering that directly represses transcription of these genes. However, we show by genetic analysis that the effect of SVP cannot be fully explained by repressing known floral integrator genes. To identify additional SVP functions, we analyzed genome-wide transcriptome data and show that GIBBERELLIN 20 OXIDASE 2, which encodes an enzyme required for biosynthesis of the growth regulator gibberellin (GA), is upregulated in svp mutants. GA is known to promote flowering, and we find that svp mutants contain elevated levels of GA that correlate with GA-related phenotypes such as early flowering and organ elongation. The ga20ox2 mutation suppresses the elevated GA levels and partially suppresses the growth and early flowering phenotypes of svp mutants. In wild-type plants, SVP expression in the shoot apical meristem falls when plants are exposed to photoperiods that induce flowering, and this correlates with increased expression of GA20ox2. Mutations that impair the photoperiodic flowering pathway prevent this downregulation of SVP and the strong increase in expression of GA20ox2. We conclude that SVP delays flowering by repressing GA biosynthesis as well as integrator gene expression and that, in response to inductive photoperiods, repression of SVP contributes to the rise in GA at the shoot apex, promoting rapid induction of flowering.
Fil: Andrés, Fernando. Max Planck Institute for Plant Breeding Research; Alemania
Fil: Porri, Aimone. Max Planck Institute for Plant Breeding Research; Alemania
Fil: Torti, Stefano. Max Planck Institute for Plant Breeding Research; Alemania
Fil: Mateos, Julieta Lisa. Max Planck Institute for Plant Breeding Research; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Fundación Instituto Leloir; Argentina
Fil: Romera Branchat, Maida. Max Planck Institute for Plant Breeding Research; Alemania
Fil: García Martínez, José Luis. Universidad Politécnica de Valencia. Instituto de Biología Molecular y Celular de Plantas; España
Fil: Fornara, Fabio. Università degli Studi di Milano. Department of Bioscience; Italia. Max Planck Institute for Plant Breeding Research; Alemania
Fil: Gregis, Veronica. Università degli Studi di Milano. Department of Bioscience; Italia
Fil: Kater, Martin M.. Università degli Studi di Milano. Department of Bioscience; Italia
Fil: Coupland, George. Max Planck Institute for Plant Breeding Research; Alemania
description In Arabidopsis thaliana environmental and endogenous cues promote flowering by activating expression of a small number of integrator genes. The MADS box transcription factor SHORT VEGETATIVE PHASE (SVP) is a critical inhibitor of flowering that directly represses transcription of these genes. However, we show by genetic analysis that the effect of SVP cannot be fully explained by repressing known floral integrator genes. To identify additional SVP functions, we analyzed genome-wide transcriptome data and show that GIBBERELLIN 20 OXIDASE 2, which encodes an enzyme required for biosynthesis of the growth regulator gibberellin (GA), is upregulated in svp mutants. GA is known to promote flowering, and we find that svp mutants contain elevated levels of GA that correlate with GA-related phenotypes such as early flowering and organ elongation. The ga20ox2 mutation suppresses the elevated GA levels and partially suppresses the growth and early flowering phenotypes of svp mutants. In wild-type plants, SVP expression in the shoot apical meristem falls when plants are exposed to photoperiods that induce flowering, and this correlates with increased expression of GA20ox2. Mutations that impair the photoperiodic flowering pathway prevent this downregulation of SVP and the strong increase in expression of GA20ox2. We conclude that SVP delays flowering by repressing GA biosynthesis as well as integrator gene expression and that, in response to inductive photoperiods, repression of SVP contributes to the rise in GA at the shoot apex, promoting rapid induction of flowering.
publishDate 2014
dc.date.none.fl_str_mv 2014-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/8347
Andrés, Fernando; Porri, Aimone; Torti, Stefano; Mateos, Julieta Lisa; Romera Branchat, Maida; et al.; Short vegetative phase reduces gibberellin biosynthesis at the arabidopsis shoot apex to regulate the floral transition; National Academy Of Sciences; Proceedings Of The National Academy Of Sciences Of The United States Of America; 111; 26; 6-2014; E2760-E2769
0027-8424
url http://hdl.handle.net/11336/8347
identifier_str_mv Andrés, Fernando; Porri, Aimone; Torti, Stefano; Mateos, Julieta Lisa; Romera Branchat, Maida; et al.; Short vegetative phase reduces gibberellin biosynthesis at the arabidopsis shoot apex to regulate the floral transition; National Academy Of Sciences; Proceedings Of The National Academy Of Sciences Of The United States Of America; 111; 26; 6-2014; E2760-E2769
0027-8424
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.pnas.org/content/111/26/E2760
info:eu-repo/semantics/altIdentifier/doi/10.1073/pnas.1409567111
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv National Academy Of Sciences
publisher.none.fl_str_mv National Academy Of Sciences
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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