Role of skeletal muscle on impaired insulin sensitivity in rats fed a sucrose-rich diet: Effect of moderate levels of dietary fish oil
- Autores
- D'alessandro, Maria Eugenia Guadalupe; Chicco, Adriana Graciela; Karabatas, Liliana Margarita; Lombardo, Yolanda
- Año de publicación
- 2000
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- In the present study we investigated: (1) the contribution of the skeletal muscle to the mechanisms underlying the impaired glucose homeostasis and insulin sensitivity present in dyslipemic rats fed a sucrose-rich diet (SRD) over a long period of time and (2) the effect of fish oil on these parameters when there was a stable hypertriglyceridemia before the source of fat (corn oil) in the diet was replaced by isocaloric amounts of cod liver oil. Our results show an increased triglyceride content in the gastrocnemius muscle with an impaired capacity for glucose oxidation in the basal state and during euglycemic clamp. This was mainly due to a decrease of the active form of pyruvate dehydrogenase complex (PDHa) and an increase of PDH kinase activities. Hyperglycemia, normoinsulinemia, and diminished peripheral insulin sensitivity also were found. Even though there were no changes in the insulin levels, the former metabolic abnormalities were completely reversed when the source of fat was changed from corn oil to cod liver oil. The data also suggest that in the gastrocnemius muscle of rats fed a SRD over an extended period, an increased availability and oxidation of the lipid fuel, which in turn impairs the glucose oxidation, contributes to the abnormal glucose homeostasis and to the peripheral insulin insensitivity. Moreover, the parallel effect on insulin sensitivity, glucose, and lipid homeostasis attained through the manipulation of dietary fat (n-3) in the SRD suggests a role of n-3 fatty acid in the management of dyslipidemia and insulin resistance. Copyright (C) 2000 Elsevier Science Inc.
Fil: D'alessandro, Maria Eugenia Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral; Argentina
Fil: Chicco, Adriana Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral; Argentina
Fil: Karabatas, Liliana Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Gobierno de la Ciudad de Buenos Aires. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Fundación de Endocrinología Infantil. Centro de Investigaciones Endocrinológicas "Dr. César Bergada"; Argentina
Fil: Lombardo, Yolanda. Universidad Nacional del Litoral; Argentina - Materia
-
(N-3) Fatty Acids
Dyslipemia
Fish Oil
Insulin Resistance
Skeletal Muscle
Sucrose-Rich Diet - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/72037
Ver los metadatos del registro completo
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CONICET Digital (CONICET) |
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Role of skeletal muscle on impaired insulin sensitivity in rats fed a sucrose-rich diet: Effect of moderate levels of dietary fish oilD'alessandro, Maria Eugenia GuadalupeChicco, Adriana GracielaKarabatas, Liliana MargaritaLombardo, Yolanda(N-3) Fatty AcidsDyslipemiaFish OilInsulin ResistanceSkeletal MuscleSucrose-Rich Diethttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3In the present study we investigated: (1) the contribution of the skeletal muscle to the mechanisms underlying the impaired glucose homeostasis and insulin sensitivity present in dyslipemic rats fed a sucrose-rich diet (SRD) over a long period of time and (2) the effect of fish oil on these parameters when there was a stable hypertriglyceridemia before the source of fat (corn oil) in the diet was replaced by isocaloric amounts of cod liver oil. Our results show an increased triglyceride content in the gastrocnemius muscle with an impaired capacity for glucose oxidation in the basal state and during euglycemic clamp. This was mainly due to a decrease of the active form of pyruvate dehydrogenase complex (PDHa) and an increase of PDH kinase activities. Hyperglycemia, normoinsulinemia, and diminished peripheral insulin sensitivity also were found. Even though there were no changes in the insulin levels, the former metabolic abnormalities were completely reversed when the source of fat was changed from corn oil to cod liver oil. The data also suggest that in the gastrocnemius muscle of rats fed a SRD over an extended period, an increased availability and oxidation of the lipid fuel, which in turn impairs the glucose oxidation, contributes to the abnormal glucose homeostasis and to the peripheral insulin insensitivity. Moreover, the parallel effect on insulin sensitivity, glucose, and lipid homeostasis attained through the manipulation of dietary fat (n-3) in the SRD suggests a role of n-3 fatty acid in the management of dyslipidemia and insulin resistance. Copyright (C) 2000 Elsevier Science Inc.Fil: D'alessandro, Maria Eugenia Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral; ArgentinaFil: Chicco, Adriana Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral; ArgentinaFil: Karabatas, Liliana Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Gobierno de la Ciudad de Buenos Aires. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Fundación de Endocrinología Infantil. Centro de Investigaciones Endocrinológicas "Dr. César Bergada"; ArgentinaFil: Lombardo, Yolanda. Universidad Nacional del Litoral; ArgentinaElsevier Science Inc2000-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/72037D'alessandro, Maria Eugenia Guadalupe; Chicco, Adriana Graciela; Karabatas, Liliana Margarita; Lombardo, Yolanda; Role of skeletal muscle on impaired insulin sensitivity in rats fed a sucrose-rich diet: Effect of moderate levels of dietary fish oil; Elsevier Science Inc; Journal Of Nutritional Biochemistry; 11; 5; 5-2000; 273-2800955-2863CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0955286300000784info:eu-repo/semantics/altIdentifier/doi/10.1016/S0955-2863(00)00078-4info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:09:53Zoai:ri.conicet.gov.ar:11336/72037instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:09:53.43CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Role of skeletal muscle on impaired insulin sensitivity in rats fed a sucrose-rich diet: Effect of moderate levels of dietary fish oil |
title |
Role of skeletal muscle on impaired insulin sensitivity in rats fed a sucrose-rich diet: Effect of moderate levels of dietary fish oil |
spellingShingle |
Role of skeletal muscle on impaired insulin sensitivity in rats fed a sucrose-rich diet: Effect of moderate levels of dietary fish oil D'alessandro, Maria Eugenia Guadalupe (N-3) Fatty Acids Dyslipemia Fish Oil Insulin Resistance Skeletal Muscle Sucrose-Rich Diet |
title_short |
Role of skeletal muscle on impaired insulin sensitivity in rats fed a sucrose-rich diet: Effect of moderate levels of dietary fish oil |
title_full |
Role of skeletal muscle on impaired insulin sensitivity in rats fed a sucrose-rich diet: Effect of moderate levels of dietary fish oil |
title_fullStr |
Role of skeletal muscle on impaired insulin sensitivity in rats fed a sucrose-rich diet: Effect of moderate levels of dietary fish oil |
title_full_unstemmed |
Role of skeletal muscle on impaired insulin sensitivity in rats fed a sucrose-rich diet: Effect of moderate levels of dietary fish oil |
title_sort |
Role of skeletal muscle on impaired insulin sensitivity in rats fed a sucrose-rich diet: Effect of moderate levels of dietary fish oil |
dc.creator.none.fl_str_mv |
D'alessandro, Maria Eugenia Guadalupe Chicco, Adriana Graciela Karabatas, Liliana Margarita Lombardo, Yolanda |
author |
D'alessandro, Maria Eugenia Guadalupe |
author_facet |
D'alessandro, Maria Eugenia Guadalupe Chicco, Adriana Graciela Karabatas, Liliana Margarita Lombardo, Yolanda |
author_role |
author |
author2 |
Chicco, Adriana Graciela Karabatas, Liliana Margarita Lombardo, Yolanda |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
(N-3) Fatty Acids Dyslipemia Fish Oil Insulin Resistance Skeletal Muscle Sucrose-Rich Diet |
topic |
(N-3) Fatty Acids Dyslipemia Fish Oil Insulin Resistance Skeletal Muscle Sucrose-Rich Diet |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.3 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
In the present study we investigated: (1) the contribution of the skeletal muscle to the mechanisms underlying the impaired glucose homeostasis and insulin sensitivity present in dyslipemic rats fed a sucrose-rich diet (SRD) over a long period of time and (2) the effect of fish oil on these parameters when there was a stable hypertriglyceridemia before the source of fat (corn oil) in the diet was replaced by isocaloric amounts of cod liver oil. Our results show an increased triglyceride content in the gastrocnemius muscle with an impaired capacity for glucose oxidation in the basal state and during euglycemic clamp. This was mainly due to a decrease of the active form of pyruvate dehydrogenase complex (PDHa) and an increase of PDH kinase activities. Hyperglycemia, normoinsulinemia, and diminished peripheral insulin sensitivity also were found. Even though there were no changes in the insulin levels, the former metabolic abnormalities were completely reversed when the source of fat was changed from corn oil to cod liver oil. The data also suggest that in the gastrocnemius muscle of rats fed a SRD over an extended period, an increased availability and oxidation of the lipid fuel, which in turn impairs the glucose oxidation, contributes to the abnormal glucose homeostasis and to the peripheral insulin insensitivity. Moreover, the parallel effect on insulin sensitivity, glucose, and lipid homeostasis attained through the manipulation of dietary fat (n-3) in the SRD suggests a role of n-3 fatty acid in the management of dyslipidemia and insulin resistance. Copyright (C) 2000 Elsevier Science Inc. Fil: D'alessandro, Maria Eugenia Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral; Argentina Fil: Chicco, Adriana Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral; Argentina Fil: Karabatas, Liliana Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Gobierno de la Ciudad de Buenos Aires. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Fundación de Endocrinología Infantil. Centro de Investigaciones Endocrinológicas "Dr. César Bergada"; Argentina Fil: Lombardo, Yolanda. Universidad Nacional del Litoral; Argentina |
description |
In the present study we investigated: (1) the contribution of the skeletal muscle to the mechanisms underlying the impaired glucose homeostasis and insulin sensitivity present in dyslipemic rats fed a sucrose-rich diet (SRD) over a long period of time and (2) the effect of fish oil on these parameters when there was a stable hypertriglyceridemia before the source of fat (corn oil) in the diet was replaced by isocaloric amounts of cod liver oil. Our results show an increased triglyceride content in the gastrocnemius muscle with an impaired capacity for glucose oxidation in the basal state and during euglycemic clamp. This was mainly due to a decrease of the active form of pyruvate dehydrogenase complex (PDHa) and an increase of PDH kinase activities. Hyperglycemia, normoinsulinemia, and diminished peripheral insulin sensitivity also were found. Even though there were no changes in the insulin levels, the former metabolic abnormalities were completely reversed when the source of fat was changed from corn oil to cod liver oil. The data also suggest that in the gastrocnemius muscle of rats fed a SRD over an extended period, an increased availability and oxidation of the lipid fuel, which in turn impairs the glucose oxidation, contributes to the abnormal glucose homeostasis and to the peripheral insulin insensitivity. Moreover, the parallel effect on insulin sensitivity, glucose, and lipid homeostasis attained through the manipulation of dietary fat (n-3) in the SRD suggests a role of n-3 fatty acid in the management of dyslipidemia and insulin resistance. Copyright (C) 2000 Elsevier Science Inc. |
publishDate |
2000 |
dc.date.none.fl_str_mv |
2000-05 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/72037 D'alessandro, Maria Eugenia Guadalupe; Chicco, Adriana Graciela; Karabatas, Liliana Margarita; Lombardo, Yolanda; Role of skeletal muscle on impaired insulin sensitivity in rats fed a sucrose-rich diet: Effect of moderate levels of dietary fish oil; Elsevier Science Inc; Journal Of Nutritional Biochemistry; 11; 5; 5-2000; 273-280 0955-2863 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/72037 |
identifier_str_mv |
D'alessandro, Maria Eugenia Guadalupe; Chicco, Adriana Graciela; Karabatas, Liliana Margarita; Lombardo, Yolanda; Role of skeletal muscle on impaired insulin sensitivity in rats fed a sucrose-rich diet: Effect of moderate levels of dietary fish oil; Elsevier Science Inc; Journal Of Nutritional Biochemistry; 11; 5; 5-2000; 273-280 0955-2863 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0955286300000784 info:eu-repo/semantics/altIdentifier/doi/10.1016/S0955-2863(00)00078-4 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Science Inc |
publisher.none.fl_str_mv |
Elsevier Science Inc |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842270097899520000 |
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13.13397 |