Role of skeletal muscle on impaired insulin sensitivity in rats fed a sucrose-rich diet: Effect of moderate levels of dietary fish oil

Autores
D'alessandro, Maria Eugenia Guadalupe; Chicco, Adriana Graciela; Karabatas, Liliana Margarita; Lombardo, Yolanda
Año de publicación
2000
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In the present study we investigated: (1) the contribution of the skeletal muscle to the mechanisms underlying the impaired glucose homeostasis and insulin sensitivity present in dyslipemic rats fed a sucrose-rich diet (SRD) over a long period of time and (2) the effect of fish oil on these parameters when there was a stable hypertriglyceridemia before the source of fat (corn oil) in the diet was replaced by isocaloric amounts of cod liver oil. Our results show an increased triglyceride content in the gastrocnemius muscle with an impaired capacity for glucose oxidation in the basal state and during euglycemic clamp. This was mainly due to a decrease of the active form of pyruvate dehydrogenase complex (PDHa) and an increase of PDH kinase activities. Hyperglycemia, normoinsulinemia, and diminished peripheral insulin sensitivity also were found. Even though there were no changes in the insulin levels, the former metabolic abnormalities were completely reversed when the source of fat was changed from corn oil to cod liver oil. The data also suggest that in the gastrocnemius muscle of rats fed a SRD over an extended period, an increased availability and oxidation of the lipid fuel, which in turn impairs the glucose oxidation, contributes to the abnormal glucose homeostasis and to the peripheral insulin insensitivity. Moreover, the parallel effect on insulin sensitivity, glucose, and lipid homeostasis attained through the manipulation of dietary fat (n-3) in the SRD suggests a role of n-3 fatty acid in the management of dyslipidemia and insulin resistance. Copyright (C) 2000 Elsevier Science Inc.
Fil: D'alessandro, Maria Eugenia Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral; Argentina
Fil: Chicco, Adriana Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral; Argentina
Fil: Karabatas, Liliana Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Gobierno de la Ciudad de Buenos Aires. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Fundación de Endocrinología Infantil. Centro de Investigaciones Endocrinológicas "Dr. César Bergada"; Argentina
Fil: Lombardo, Yolanda. Universidad Nacional del Litoral; Argentina
Materia
(N-3) Fatty Acids
Dyslipemia
Fish Oil
Insulin Resistance
Skeletal Muscle
Sucrose-Rich Diet
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/72037
Acceder
id CONICETDig_967d26d59c9f8cd10d694571a2f9e0f6
oai_identifier_str oai:ri.conicet.gov.ar:11336/72037
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Role of skeletal muscle on impaired insulin sensitivity in rats fed a sucrose-rich diet: Effect of moderate levels of dietary fish oilD'alessandro, Maria Eugenia GuadalupeChicco, Adriana GracielaKarabatas, Liliana MargaritaLombardo, Yolanda(N-3) Fatty AcidsDyslipemiaFish OilInsulin ResistanceSkeletal MuscleSucrose-Rich Diethttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3In the present study we investigated: (1) the contribution of the skeletal muscle to the mechanisms underlying the impaired glucose homeostasis and insulin sensitivity present in dyslipemic rats fed a sucrose-rich diet (SRD) over a long period of time and (2) the effect of fish oil on these parameters when there was a stable hypertriglyceridemia before the source of fat (corn oil) in the diet was replaced by isocaloric amounts of cod liver oil. Our results show an increased triglyceride content in the gastrocnemius muscle with an impaired capacity for glucose oxidation in the basal state and during euglycemic clamp. This was mainly due to a decrease of the active form of pyruvate dehydrogenase complex (PDHa) and an increase of PDH kinase activities. Hyperglycemia, normoinsulinemia, and diminished peripheral insulin sensitivity also were found. Even though there were no changes in the insulin levels, the former metabolic abnormalities were completely reversed when the source of fat was changed from corn oil to cod liver oil. The data also suggest that in the gastrocnemius muscle of rats fed a SRD over an extended period, an increased availability and oxidation of the lipid fuel, which in turn impairs the glucose oxidation, contributes to the abnormal glucose homeostasis and to the peripheral insulin insensitivity. Moreover, the parallel effect on insulin sensitivity, glucose, and lipid homeostasis attained through the manipulation of dietary fat (n-3) in the SRD suggests a role of n-3 fatty acid in the management of dyslipidemia and insulin resistance. Copyright (C) 2000 Elsevier Science Inc.Fil: D'alessandro, Maria Eugenia Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral; ArgentinaFil: Chicco, Adriana Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral; ArgentinaFil: Karabatas, Liliana Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Gobierno de la Ciudad de Buenos Aires. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Fundación de Endocrinología Infantil. Centro de Investigaciones Endocrinológicas "Dr. César Bergada"; ArgentinaFil: Lombardo, Yolanda. Universidad Nacional del Litoral; ArgentinaElsevier Science Inc2000-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/72037D'alessandro, Maria Eugenia Guadalupe; Chicco, Adriana Graciela; Karabatas, Liliana Margarita; Lombardo, Yolanda; Role of skeletal muscle on impaired insulin sensitivity in rats fed a sucrose-rich diet: Effect of moderate levels of dietary fish oil; Elsevier Science Inc; Journal Of Nutritional Biochemistry; 11; 5; 5-2000; 273-2800955-2863CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0955286300000784info:eu-repo/semantics/altIdentifier/doi/10.1016/S0955-2863(00)00078-4info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:41:09Zoai:ri.conicet.gov.ar:11336/72037instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:41:09.267CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Role of skeletal muscle on impaired insulin sensitivity in rats fed a sucrose-rich diet: Effect of moderate levels of dietary fish oil
title Role of skeletal muscle on impaired insulin sensitivity in rats fed a sucrose-rich diet: Effect of moderate levels of dietary fish oil
spellingShingle Role of skeletal muscle on impaired insulin sensitivity in rats fed a sucrose-rich diet: Effect of moderate levels of dietary fish oil
D'alessandro, Maria Eugenia Guadalupe
(N-3) Fatty Acids
Dyslipemia
Fish Oil
Insulin Resistance
Skeletal Muscle
Sucrose-Rich Diet
title_short Role of skeletal muscle on impaired insulin sensitivity in rats fed a sucrose-rich diet: Effect of moderate levels of dietary fish oil
title_full Role of skeletal muscle on impaired insulin sensitivity in rats fed a sucrose-rich diet: Effect of moderate levels of dietary fish oil
title_fullStr Role of skeletal muscle on impaired insulin sensitivity in rats fed a sucrose-rich diet: Effect of moderate levels of dietary fish oil
title_full_unstemmed Role of skeletal muscle on impaired insulin sensitivity in rats fed a sucrose-rich diet: Effect of moderate levels of dietary fish oil
title_sort Role of skeletal muscle on impaired insulin sensitivity in rats fed a sucrose-rich diet: Effect of moderate levels of dietary fish oil
dc.creator.none.fl_str_mv D'alessandro, Maria Eugenia Guadalupe
Chicco, Adriana Graciela
Karabatas, Liliana Margarita
Lombardo, Yolanda
author D'alessandro, Maria Eugenia Guadalupe
author_facet D'alessandro, Maria Eugenia Guadalupe
Chicco, Adriana Graciela
Karabatas, Liliana Margarita
Lombardo, Yolanda
author_role author
author2 Chicco, Adriana Graciela
Karabatas, Liliana Margarita
Lombardo, Yolanda
author2_role author
author
author
dc.subject.none.fl_str_mv (N-3) Fatty Acids
Dyslipemia
Fish Oil
Insulin Resistance
Skeletal Muscle
Sucrose-Rich Diet
topic (N-3) Fatty Acids
Dyslipemia
Fish Oil
Insulin Resistance
Skeletal Muscle
Sucrose-Rich Diet
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv In the present study we investigated: (1) the contribution of the skeletal muscle to the mechanisms underlying the impaired glucose homeostasis and insulin sensitivity present in dyslipemic rats fed a sucrose-rich diet (SRD) over a long period of time and (2) the effect of fish oil on these parameters when there was a stable hypertriglyceridemia before the source of fat (corn oil) in the diet was replaced by isocaloric amounts of cod liver oil. Our results show an increased triglyceride content in the gastrocnemius muscle with an impaired capacity for glucose oxidation in the basal state and during euglycemic clamp. This was mainly due to a decrease of the active form of pyruvate dehydrogenase complex (PDHa) and an increase of PDH kinase activities. Hyperglycemia, normoinsulinemia, and diminished peripheral insulin sensitivity also were found. Even though there were no changes in the insulin levels, the former metabolic abnormalities were completely reversed when the source of fat was changed from corn oil to cod liver oil. The data also suggest that in the gastrocnemius muscle of rats fed a SRD over an extended period, an increased availability and oxidation of the lipid fuel, which in turn impairs the glucose oxidation, contributes to the abnormal glucose homeostasis and to the peripheral insulin insensitivity. Moreover, the parallel effect on insulin sensitivity, glucose, and lipid homeostasis attained through the manipulation of dietary fat (n-3) in the SRD suggests a role of n-3 fatty acid in the management of dyslipidemia and insulin resistance. Copyright (C) 2000 Elsevier Science Inc.
Fil: D'alessandro, Maria Eugenia Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral; Argentina
Fil: Chicco, Adriana Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral; Argentina
Fil: Karabatas, Liliana Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Gobierno de la Ciudad de Buenos Aires. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Fundación de Endocrinología Infantil. Centro de Investigaciones Endocrinológicas "Dr. César Bergada"; Argentina
Fil: Lombardo, Yolanda. Universidad Nacional del Litoral; Argentina
description In the present study we investigated: (1) the contribution of the skeletal muscle to the mechanisms underlying the impaired glucose homeostasis and insulin sensitivity present in dyslipemic rats fed a sucrose-rich diet (SRD) over a long period of time and (2) the effect of fish oil on these parameters when there was a stable hypertriglyceridemia before the source of fat (corn oil) in the diet was replaced by isocaloric amounts of cod liver oil. Our results show an increased triglyceride content in the gastrocnemius muscle with an impaired capacity for glucose oxidation in the basal state and during euglycemic clamp. This was mainly due to a decrease of the active form of pyruvate dehydrogenase complex (PDHa) and an increase of PDH kinase activities. Hyperglycemia, normoinsulinemia, and diminished peripheral insulin sensitivity also were found. Even though there were no changes in the insulin levels, the former metabolic abnormalities were completely reversed when the source of fat was changed from corn oil to cod liver oil. The data also suggest that in the gastrocnemius muscle of rats fed a SRD over an extended period, an increased availability and oxidation of the lipid fuel, which in turn impairs the glucose oxidation, contributes to the abnormal glucose homeostasis and to the peripheral insulin insensitivity. Moreover, the parallel effect on insulin sensitivity, glucose, and lipid homeostasis attained through the manipulation of dietary fat (n-3) in the SRD suggests a role of n-3 fatty acid in the management of dyslipidemia and insulin resistance. Copyright (C) 2000 Elsevier Science Inc.
publishDate 2000
dc.date.none.fl_str_mv 2000-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/72037
D'alessandro, Maria Eugenia Guadalupe; Chicco, Adriana Graciela; Karabatas, Liliana Margarita; Lombardo, Yolanda; Role of skeletal muscle on impaired insulin sensitivity in rats fed a sucrose-rich diet: Effect of moderate levels of dietary fish oil; Elsevier Science Inc; Journal Of Nutritional Biochemistry; 11; 5; 5-2000; 273-280
0955-2863
CONICET Digital
CONICET
url http://hdl.handle.net/11336/72037
identifier_str_mv D'alessandro, Maria Eugenia Guadalupe; Chicco, Adriana Graciela; Karabatas, Liliana Margarita; Lombardo, Yolanda; Role of skeletal muscle on impaired insulin sensitivity in rats fed a sucrose-rich diet: Effect of moderate levels of dietary fish oil; Elsevier Science Inc; Journal Of Nutritional Biochemistry; 11; 5; 5-2000; 273-280
0955-2863
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0955286300000784
info:eu-repo/semantics/altIdentifier/doi/10.1016/S0955-2863(00)00078-4
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Inc
publisher.none.fl_str_mv Elsevier Science Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614441532915712
score 13.069144

Publicaciones similares