Therapeutic exercise attenuates neutrophilic lung injury and skeletal muscle wasting

Autores
Files, D. Clark; Liu, Chun; Pereyra, Andrea Soledad; Wang, Zhong Min; Aggarwal, Neil; D´Alessio, Franco; Garibaldi, Brian T.; Mock, Jason R.; Singer, Benjamin D.; Feng, Xin; Yammani, Raghunatha R.; Zhang, Tan; Lee, Amy L.; Philpott, Sydney; Lussier, Stephanie; Purcell, Lina; Chou, Jeff; Seeds, Michael; King, Landon S.; Morris, Peter E.; Delbono, Osvaldo
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Early mobilization of critically ill patients with the acute respiratory distress syndrome (ARDS) has emerged as a therapeutic strategy that improves patient outcomes, such as the duration of mechanical ventilation and muscle strength. Despite the apparent efficacy of early mobility programs, their use in clinical practice is limited outside of specialized centers and clinical trials. To evaluate the mechanisms underlying mobility therapy, we exercised acute lung injury (ALI) mice for 2 days after the instillation of lipopolysaccharides into their lungs. We found that a short duration of moderate intensity exercise in ALI mice attenuated muscle ring finger 1 (MuRF1)?mediated atrophy of the limb and respiratory muscles and improved limb muscle force generation. Exercise also limited the influx of neutrophils into the alveolar space through modulation of a coordinated systemic neutrophil chemokine response. Granulocyte colony-stimulating factor (G-CSF) concentrations were systemically reduced by exercise in ALI mice, and in vivo blockade of the G-CSF receptor recapitulated the lung exercise phenotype in ALI mice. Additionally, plasma G-CSF concentrations in humans with acute respiratory failure (ARF) undergoing early mobility therapy showed greater decrements over time compared to control ARF patients. Together, these data provide a mechanism whereby early mobility therapy attenuates muscle wasting and limits ongoing alveolar neutrophilia through modulation of systemic neutrophil chemokines in lung-injured mice and humans.
Fil: Files, D. Clark. School Of Medicine; Estados Unidos
Fil: Liu, Chun. School Of Medicine; Estados Unidos
Fil: Pereyra, Andrea Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Fil: Wang, Zhong Min. University Wake Forest; Estados Unidos. School Of Medicine; Estados Unidos
Fil: Aggarwal, Neil. Johns Hopkins Asthma And Allergy Center; Estados Unidos
Fil: D´Alessio, Franco. Johns Hopkins Asthma And Allergy Center; Estados Unidos
Fil: Garibaldi, Brian T.. Johns Hopkins Asthma and Allergy Center; Estados Unidos
Fil: Mock, Jason R.. Johns Hopkins Asthma and Allergy Center; Estados Unidos
Fil: Singer, Benjamin D.. Johns Hopkins Asthma and Allergy Center; Estados Unidos
Fil: Feng, Xin. Wake Forest School of Medicine; Estados Unidos
Fil: Yammani, Raghunatha R.. Wake Forest School of Medicine; Estados Unidos
Fil: Zhang, Tan. Wake Forest School of Medicine; Estados Unidos
Fil: Lee, Amy L.. Wake Forest School of Medicine; Estados Unidos
Fil: Philpott, Sydney. Wake Forest School of Medicine; Estados Unidos
Fil: Lussier, Stephanie. Wake Forest School of Medicine; Estados Unidos
Fil: Purcell, Lina. Wake Forest School of Medicine; Estados Unidos
Fil: Chou, Jeff. Wake Forest School of Medicine; Estados Unidos
Fil: Seeds, Michael. Wake Forest School of Medicine; Estados Unidos
Fil: King, Landon S.. Johns Hopkins Asthma and Allergy Center; Estados Unidos
Fil: Morris, Peter E.. Wake Forest School of Medicine; Estados Unidos
Fil: Delbono, Osvaldo. School Of Medicine; Estados Unidos
Materia
LUNG INJURY
EXERCISE
SKELETAL MUSCLE WASTING
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/125093

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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Therapeutic exercise attenuates neutrophilic lung injury and skeletal muscle wastingFiles, D. ClarkLiu, ChunPereyra, Andrea SoledadWang, Zhong MinAggarwal, NeilD´Alessio, FrancoGaribaldi, Brian T.Mock, Jason R.Singer, Benjamin D.Feng, XinYammani, Raghunatha R.Zhang, TanLee, Amy L.Philpott, SydneyLussier, StephaniePurcell, LinaChou, JeffSeeds, MichaelKing, Landon S.Morris, Peter E.Delbono, OsvaldoLUNG INJURYEXERCISESKELETAL MUSCLE WASTINGhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Early mobilization of critically ill patients with the acute respiratory distress syndrome (ARDS) has emerged as a therapeutic strategy that improves patient outcomes, such as the duration of mechanical ventilation and muscle strength. Despite the apparent efficacy of early mobility programs, their use in clinical practice is limited outside of specialized centers and clinical trials. To evaluate the mechanisms underlying mobility therapy, we exercised acute lung injury (ALI) mice for 2 days after the instillation of lipopolysaccharides into their lungs. We found that a short duration of moderate intensity exercise in ALI mice attenuated muscle ring finger 1 (MuRF1)?mediated atrophy of the limb and respiratory muscles and improved limb muscle force generation. Exercise also limited the influx of neutrophils into the alveolar space through modulation of a coordinated systemic neutrophil chemokine response. Granulocyte colony-stimulating factor (G-CSF) concentrations were systemically reduced by exercise in ALI mice, and in vivo blockade of the G-CSF receptor recapitulated the lung exercise phenotype in ALI mice. Additionally, plasma G-CSF concentrations in humans with acute respiratory failure (ARF) undergoing early mobility therapy showed greater decrements over time compared to control ARF patients. Together, these data provide a mechanism whereby early mobility therapy attenuates muscle wasting and limits ongoing alveolar neutrophilia through modulation of systemic neutrophil chemokines in lung-injured mice and humans.Fil: Files, D. Clark. School Of Medicine; Estados UnidosFil: Liu, Chun. School Of Medicine; Estados UnidosFil: Pereyra, Andrea Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; ArgentinaFil: Wang, Zhong Min. University Wake Forest; Estados Unidos. School Of Medicine; Estados UnidosFil: Aggarwal, Neil. Johns Hopkins Asthma And Allergy Center; Estados UnidosFil: D´Alessio, Franco. Johns Hopkins Asthma And Allergy Center; Estados UnidosFil: Garibaldi, Brian T.. Johns Hopkins Asthma and Allergy Center; Estados UnidosFil: Mock, Jason R.. Johns Hopkins Asthma and Allergy Center; Estados UnidosFil: Singer, Benjamin D.. Johns Hopkins Asthma and Allergy Center; Estados UnidosFil: Feng, Xin. Wake Forest School of Medicine; Estados UnidosFil: Yammani, Raghunatha R.. Wake Forest School of Medicine; Estados UnidosFil: Zhang, Tan. Wake Forest School of Medicine; Estados UnidosFil: Lee, Amy L.. Wake Forest School of Medicine; Estados UnidosFil: Philpott, Sydney. Wake Forest School of Medicine; Estados UnidosFil: Lussier, Stephanie. Wake Forest School of Medicine; Estados UnidosFil: Purcell, Lina. Wake Forest School of Medicine; Estados UnidosFil: Chou, Jeff. Wake Forest School of Medicine; Estados UnidosFil: Seeds, Michael. Wake Forest School of Medicine; Estados UnidosFil: King, Landon S.. Johns Hopkins Asthma and Allergy Center; Estados UnidosFil: Morris, Peter E.. Wake Forest School of Medicine; Estados UnidosFil: Delbono, Osvaldo. School Of Medicine; Estados UnidosAmerican Association for the Advancement of Science2015-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/125093Files, D. Clark; Liu, Chun; Pereyra, Andrea Soledad; Wang, Zhong Min; Aggarwal, Neil; et al.; Therapeutic exercise attenuates neutrophilic lung injury and skeletal muscle wasting; American Association for the Advancement of Science; SCIENCE TRANSLATIONAL MEDICINE; 7; 278; 3-2015; 278-3101946-6234CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://stm.sciencemag.org/content/7/278/278ra32info:eu-repo/semantics/altIdentifier/doi/10.1126/scitranslmed.3010283info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4820823/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:06:07Zoai:ri.conicet.gov.ar:11336/125093instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:06:08.094CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Therapeutic exercise attenuates neutrophilic lung injury and skeletal muscle wasting
title Therapeutic exercise attenuates neutrophilic lung injury and skeletal muscle wasting
spellingShingle Therapeutic exercise attenuates neutrophilic lung injury and skeletal muscle wasting
Files, D. Clark
LUNG INJURY
EXERCISE
SKELETAL MUSCLE WASTING
title_short Therapeutic exercise attenuates neutrophilic lung injury and skeletal muscle wasting
title_full Therapeutic exercise attenuates neutrophilic lung injury and skeletal muscle wasting
title_fullStr Therapeutic exercise attenuates neutrophilic lung injury and skeletal muscle wasting
title_full_unstemmed Therapeutic exercise attenuates neutrophilic lung injury and skeletal muscle wasting
title_sort Therapeutic exercise attenuates neutrophilic lung injury and skeletal muscle wasting
dc.creator.none.fl_str_mv Files, D. Clark
Liu, Chun
Pereyra, Andrea Soledad
Wang, Zhong Min
Aggarwal, Neil
D´Alessio, Franco
Garibaldi, Brian T.
Mock, Jason R.
Singer, Benjamin D.
Feng, Xin
Yammani, Raghunatha R.
Zhang, Tan
Lee, Amy L.
Philpott, Sydney
Lussier, Stephanie
Purcell, Lina
Chou, Jeff
Seeds, Michael
King, Landon S.
Morris, Peter E.
Delbono, Osvaldo
author Files, D. Clark
author_facet Files, D. Clark
Liu, Chun
Pereyra, Andrea Soledad
Wang, Zhong Min
Aggarwal, Neil
D´Alessio, Franco
Garibaldi, Brian T.
Mock, Jason R.
Singer, Benjamin D.
Feng, Xin
Yammani, Raghunatha R.
Zhang, Tan
Lee, Amy L.
Philpott, Sydney
Lussier, Stephanie
Purcell, Lina
Chou, Jeff
Seeds, Michael
King, Landon S.
Morris, Peter E.
Delbono, Osvaldo
author_role author
author2 Liu, Chun
Pereyra, Andrea Soledad
Wang, Zhong Min
Aggarwal, Neil
D´Alessio, Franco
Garibaldi, Brian T.
Mock, Jason R.
Singer, Benjamin D.
Feng, Xin
Yammani, Raghunatha R.
Zhang, Tan
Lee, Amy L.
Philpott, Sydney
Lussier, Stephanie
Purcell, Lina
Chou, Jeff
Seeds, Michael
King, Landon S.
Morris, Peter E.
Delbono, Osvaldo
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv LUNG INJURY
EXERCISE
SKELETAL MUSCLE WASTING
topic LUNG INJURY
EXERCISE
SKELETAL MUSCLE WASTING
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Early mobilization of critically ill patients with the acute respiratory distress syndrome (ARDS) has emerged as a therapeutic strategy that improves patient outcomes, such as the duration of mechanical ventilation and muscle strength. Despite the apparent efficacy of early mobility programs, their use in clinical practice is limited outside of specialized centers and clinical trials. To evaluate the mechanisms underlying mobility therapy, we exercised acute lung injury (ALI) mice for 2 days after the instillation of lipopolysaccharides into their lungs. We found that a short duration of moderate intensity exercise in ALI mice attenuated muscle ring finger 1 (MuRF1)?mediated atrophy of the limb and respiratory muscles and improved limb muscle force generation. Exercise also limited the influx of neutrophils into the alveolar space through modulation of a coordinated systemic neutrophil chemokine response. Granulocyte colony-stimulating factor (G-CSF) concentrations were systemically reduced by exercise in ALI mice, and in vivo blockade of the G-CSF receptor recapitulated the lung exercise phenotype in ALI mice. Additionally, plasma G-CSF concentrations in humans with acute respiratory failure (ARF) undergoing early mobility therapy showed greater decrements over time compared to control ARF patients. Together, these data provide a mechanism whereby early mobility therapy attenuates muscle wasting and limits ongoing alveolar neutrophilia through modulation of systemic neutrophil chemokines in lung-injured mice and humans.
Fil: Files, D. Clark. School Of Medicine; Estados Unidos
Fil: Liu, Chun. School Of Medicine; Estados Unidos
Fil: Pereyra, Andrea Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Fil: Wang, Zhong Min. University Wake Forest; Estados Unidos. School Of Medicine; Estados Unidos
Fil: Aggarwal, Neil. Johns Hopkins Asthma And Allergy Center; Estados Unidos
Fil: D´Alessio, Franco. Johns Hopkins Asthma And Allergy Center; Estados Unidos
Fil: Garibaldi, Brian T.. Johns Hopkins Asthma and Allergy Center; Estados Unidos
Fil: Mock, Jason R.. Johns Hopkins Asthma and Allergy Center; Estados Unidos
Fil: Singer, Benjamin D.. Johns Hopkins Asthma and Allergy Center; Estados Unidos
Fil: Feng, Xin. Wake Forest School of Medicine; Estados Unidos
Fil: Yammani, Raghunatha R.. Wake Forest School of Medicine; Estados Unidos
Fil: Zhang, Tan. Wake Forest School of Medicine; Estados Unidos
Fil: Lee, Amy L.. Wake Forest School of Medicine; Estados Unidos
Fil: Philpott, Sydney. Wake Forest School of Medicine; Estados Unidos
Fil: Lussier, Stephanie. Wake Forest School of Medicine; Estados Unidos
Fil: Purcell, Lina. Wake Forest School of Medicine; Estados Unidos
Fil: Chou, Jeff. Wake Forest School of Medicine; Estados Unidos
Fil: Seeds, Michael. Wake Forest School of Medicine; Estados Unidos
Fil: King, Landon S.. Johns Hopkins Asthma and Allergy Center; Estados Unidos
Fil: Morris, Peter E.. Wake Forest School of Medicine; Estados Unidos
Fil: Delbono, Osvaldo. School Of Medicine; Estados Unidos
description Early mobilization of critically ill patients with the acute respiratory distress syndrome (ARDS) has emerged as a therapeutic strategy that improves patient outcomes, such as the duration of mechanical ventilation and muscle strength. Despite the apparent efficacy of early mobility programs, their use in clinical practice is limited outside of specialized centers and clinical trials. To evaluate the mechanisms underlying mobility therapy, we exercised acute lung injury (ALI) mice for 2 days after the instillation of lipopolysaccharides into their lungs. We found that a short duration of moderate intensity exercise in ALI mice attenuated muscle ring finger 1 (MuRF1)?mediated atrophy of the limb and respiratory muscles and improved limb muscle force generation. Exercise also limited the influx of neutrophils into the alveolar space through modulation of a coordinated systemic neutrophil chemokine response. Granulocyte colony-stimulating factor (G-CSF) concentrations were systemically reduced by exercise in ALI mice, and in vivo blockade of the G-CSF receptor recapitulated the lung exercise phenotype in ALI mice. Additionally, plasma G-CSF concentrations in humans with acute respiratory failure (ARF) undergoing early mobility therapy showed greater decrements over time compared to control ARF patients. Together, these data provide a mechanism whereby early mobility therapy attenuates muscle wasting and limits ongoing alveolar neutrophilia through modulation of systemic neutrophil chemokines in lung-injured mice and humans.
publishDate 2015
dc.date.none.fl_str_mv 2015-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/125093
Files, D. Clark; Liu, Chun; Pereyra, Andrea Soledad; Wang, Zhong Min; Aggarwal, Neil; et al.; Therapeutic exercise attenuates neutrophilic lung injury and skeletal muscle wasting; American Association for the Advancement of Science; SCIENCE TRANSLATIONAL MEDICINE; 7; 278; 3-2015; 278-310
1946-6234
CONICET Digital
CONICET
url http://hdl.handle.net/11336/125093
identifier_str_mv Files, D. Clark; Liu, Chun; Pereyra, Andrea Soledad; Wang, Zhong Min; Aggarwal, Neil; et al.; Therapeutic exercise attenuates neutrophilic lung injury and skeletal muscle wasting; American Association for the Advancement of Science; SCIENCE TRANSLATIONAL MEDICINE; 7; 278; 3-2015; 278-310
1946-6234
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://stm.sciencemag.org/content/7/278/278ra32
info:eu-repo/semantics/altIdentifier/doi/10.1126/scitranslmed.3010283
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4820823/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Association for the Advancement of Science
publisher.none.fl_str_mv American Association for the Advancement of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
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repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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