Fish oil reverses the altered glucose transporter, phosphorylation, insulin receptor substrate-1 (IRS-1) protein level and lipid contents in skeletal muscle of sucrose-rich diet fe...
- Autores
- D'alessandro, Maria Eugenia Guadalupe; Chicco, Adriana Graciela; Lombardo, Yolanda B.
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The role and underlying mechanisms by which n−3 polyunsaturated fatty acids (PUFA) prevent/reverse SRD-induced insulin resistance (IR) in the muscle are not completely understood. Therefore, we examined: triglyceride, diacylglycerol, PKCθ, Glut-4, enzymatic hexokinase activity, IRS-1 protein mass level, and fatty acid composition of muscle phospholipids. Rats were fed a SRD during 6 months. Thereafter, half the animals continued with SRD up to 8 months; the other half was fed a SRD in which CO (8% wt/wt) was replaced by FO (7%+1% CO) for 2 months. Results were compared with those obtained in rats fed a control diet (CD). In SRD-fed rats, FO oil normalized/improved lipid storage and PKCθ protein mass level. Effects of insulin were comparable with those of CD-fed rats. FO reversed impaired glucose phosphorylation, IRS-1, and, under insulin stimulation, Glut-4 protein mass level. FO normalized insulin resistance and increased n−3 PUFAs in muscle phospholipids.
Fil: D'alessandro, Maria Eugenia Guadalupe. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe; Argentina
Fil: Chicco, Adriana Graciela. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe; Argentina
Fil: Lombardo, Yolanda B.. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina - Materia
-
Insulin Action
Dyslipidemia
Polyunsaturated Fatty Acids
Skeletal Muscle - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/6378
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Fish oil reverses the altered glucose transporter, phosphorylation, insulin receptor substrate-1 (IRS-1) protein level and lipid contents in skeletal muscle of sucrose-rich diet fed ratsD'alessandro, Maria Eugenia GuadalupeChicco, Adriana GracielaLombardo, Yolanda B.Insulin ActionDyslipidemiaPolyunsaturated Fatty AcidsSkeletal Musclehttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3The role and underlying mechanisms by which n−3 polyunsaturated fatty acids (PUFA) prevent/reverse SRD-induced insulin resistance (IR) in the muscle are not completely understood. Therefore, we examined: triglyceride, diacylglycerol, PKCθ, Glut-4, enzymatic hexokinase activity, IRS-1 protein mass level, and fatty acid composition of muscle phospholipids. Rats were fed a SRD during 6 months. Thereafter, half the animals continued with SRD up to 8 months; the other half was fed a SRD in which CO (8% wt/wt) was replaced by FO (7%+1% CO) for 2 months. Results were compared with those obtained in rats fed a control diet (CD). In SRD-fed rats, FO oil normalized/improved lipid storage and PKCθ protein mass level. Effects of insulin were comparable with those of CD-fed rats. FO reversed impaired glucose phosphorylation, IRS-1, and, under insulin stimulation, Glut-4 protein mass level. FO normalized insulin resistance and increased n−3 PUFAs in muscle phospholipids.Fil: D'alessandro, Maria Eugenia Guadalupe. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe; ArgentinaFil: Chicco, Adriana Graciela. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe; ArgentinaFil: Lombardo, Yolanda B.. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaElsevier2013-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/6378D'alessandro, Maria Eugenia Guadalupe; Chicco, Adriana Graciela; Lombardo, Yolanda B.; Fish oil reverses the altered glucose transporter, phosphorylation, insulin receptor substrate-1 (IRS-1) protein level and lipid contents in skeletal muscle of sucrose-rich diet fed rats; Elsevier; Prostaglandins Leukotrienes and Essential Fatty Acids; 88; 2; 1-2013; 171-1770952-3278enginfo:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/doi/10.1016/j.plefa.2012.11.003info:eu-repo/semantics/altIdentifier/purl/http://www.sciencedirect.com/science/article/pii/S0952327812001871info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:56:19Zoai:ri.conicet.gov.ar:11336/6378instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:56:19.59CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Fish oil reverses the altered glucose transporter, phosphorylation, insulin receptor substrate-1 (IRS-1) protein level and lipid contents in skeletal muscle of sucrose-rich diet fed rats |
title |
Fish oil reverses the altered glucose transporter, phosphorylation, insulin receptor substrate-1 (IRS-1) protein level and lipid contents in skeletal muscle of sucrose-rich diet fed rats |
spellingShingle |
Fish oil reverses the altered glucose transporter, phosphorylation, insulin receptor substrate-1 (IRS-1) protein level and lipid contents in skeletal muscle of sucrose-rich diet fed rats D'alessandro, Maria Eugenia Guadalupe Insulin Action Dyslipidemia Polyunsaturated Fatty Acids Skeletal Muscle |
title_short |
Fish oil reverses the altered glucose transporter, phosphorylation, insulin receptor substrate-1 (IRS-1) protein level and lipid contents in skeletal muscle of sucrose-rich diet fed rats |
title_full |
Fish oil reverses the altered glucose transporter, phosphorylation, insulin receptor substrate-1 (IRS-1) protein level and lipid contents in skeletal muscle of sucrose-rich diet fed rats |
title_fullStr |
Fish oil reverses the altered glucose transporter, phosphorylation, insulin receptor substrate-1 (IRS-1) protein level and lipid contents in skeletal muscle of sucrose-rich diet fed rats |
title_full_unstemmed |
Fish oil reverses the altered glucose transporter, phosphorylation, insulin receptor substrate-1 (IRS-1) protein level and lipid contents in skeletal muscle of sucrose-rich diet fed rats |
title_sort |
Fish oil reverses the altered glucose transporter, phosphorylation, insulin receptor substrate-1 (IRS-1) protein level and lipid contents in skeletal muscle of sucrose-rich diet fed rats |
dc.creator.none.fl_str_mv |
D'alessandro, Maria Eugenia Guadalupe Chicco, Adriana Graciela Lombardo, Yolanda B. |
author |
D'alessandro, Maria Eugenia Guadalupe |
author_facet |
D'alessandro, Maria Eugenia Guadalupe Chicco, Adriana Graciela Lombardo, Yolanda B. |
author_role |
author |
author2 |
Chicco, Adriana Graciela Lombardo, Yolanda B. |
author2_role |
author author |
dc.subject.none.fl_str_mv |
Insulin Action Dyslipidemia Polyunsaturated Fatty Acids Skeletal Muscle |
topic |
Insulin Action Dyslipidemia Polyunsaturated Fatty Acids Skeletal Muscle |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.3 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
The role and underlying mechanisms by which n−3 polyunsaturated fatty acids (PUFA) prevent/reverse SRD-induced insulin resistance (IR) in the muscle are not completely understood. Therefore, we examined: triglyceride, diacylglycerol, PKCθ, Glut-4, enzymatic hexokinase activity, IRS-1 protein mass level, and fatty acid composition of muscle phospholipids. Rats were fed a SRD during 6 months. Thereafter, half the animals continued with SRD up to 8 months; the other half was fed a SRD in which CO (8% wt/wt) was replaced by FO (7%+1% CO) for 2 months. Results were compared with those obtained in rats fed a control diet (CD). In SRD-fed rats, FO oil normalized/improved lipid storage and PKCθ protein mass level. Effects of insulin were comparable with those of CD-fed rats. FO reversed impaired glucose phosphorylation, IRS-1, and, under insulin stimulation, Glut-4 protein mass level. FO normalized insulin resistance and increased n−3 PUFAs in muscle phospholipids. Fil: D'alessandro, Maria Eugenia Guadalupe. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe; Argentina Fil: Chicco, Adriana Graciela. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe; Argentina Fil: Lombardo, Yolanda B.. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina |
description |
The role and underlying mechanisms by which n−3 polyunsaturated fatty acids (PUFA) prevent/reverse SRD-induced insulin resistance (IR) in the muscle are not completely understood. Therefore, we examined: triglyceride, diacylglycerol, PKCθ, Glut-4, enzymatic hexokinase activity, IRS-1 protein mass level, and fatty acid composition of muscle phospholipids. Rats were fed a SRD during 6 months. Thereafter, half the animals continued with SRD up to 8 months; the other half was fed a SRD in which CO (8% wt/wt) was replaced by FO (7%+1% CO) for 2 months. Results were compared with those obtained in rats fed a control diet (CD). In SRD-fed rats, FO oil normalized/improved lipid storage and PKCθ protein mass level. Effects of insulin were comparable with those of CD-fed rats. FO reversed impaired glucose phosphorylation, IRS-1, and, under insulin stimulation, Glut-4 protein mass level. FO normalized insulin resistance and increased n−3 PUFAs in muscle phospholipids. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-01 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/6378 D'alessandro, Maria Eugenia Guadalupe; Chicco, Adriana Graciela; Lombardo, Yolanda B.; Fish oil reverses the altered glucose transporter, phosphorylation, insulin receptor substrate-1 (IRS-1) protein level and lipid contents in skeletal muscle of sucrose-rich diet fed rats; Elsevier; Prostaglandins Leukotrienes and Essential Fatty Acids; 88; 2; 1-2013; 171-177 0952-3278 |
url |
http://hdl.handle.net/11336/6378 |
identifier_str_mv |
D'alessandro, Maria Eugenia Guadalupe; Chicco, Adriana Graciela; Lombardo, Yolanda B.; Fish oil reverses the altered glucose transporter, phosphorylation, insulin receptor substrate-1 (IRS-1) protein level and lipid contents in skeletal muscle of sucrose-rich diet fed rats; Elsevier; Prostaglandins Leukotrienes and Essential Fatty Acids; 88; 2; 1-2013; 171-177 0952-3278 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/ info:eu-repo/semantics/altIdentifier/doi/10.1016/j.plefa.2012.11.003 info:eu-repo/semantics/altIdentifier/purl/http://www.sciencedirect.com/science/article/pii/S0952327812001871 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842269397985525760 |
score |
13.13397 |