Fish oil reverses the altered glucose transporter, phosphorylation, insulin receptor substrate-1 (IRS-1) protein level and lipid contents in skeletal muscle of sucrose-rich diet fe...

Autores
D'alessandro, Maria Eugenia Guadalupe; Chicco, Adriana Graciela; Lombardo, Yolanda B.
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The role and underlying mechanisms by which n−3 polyunsaturated fatty acids (PUFA) prevent/reverse SRD-induced insulin resistance (IR) in the muscle are not completely understood. Therefore, we examined: triglyceride, diacylglycerol, PKCθ, Glut-4, enzymatic hexokinase activity, IRS-1 protein mass level, and fatty acid composition of muscle phospholipids. Rats were fed a SRD during 6 months. Thereafter, half the animals continued with SRD up to 8 months; the other half was fed a SRD in which CO (8% wt/wt) was replaced by FO (7%+1% CO) for 2 months. Results were compared with those obtained in rats fed a control diet (CD). In SRD-fed rats, FO oil normalized/improved lipid storage and PKCθ protein mass level. Effects of insulin were comparable with those of CD-fed rats. FO reversed impaired glucose phosphorylation, IRS-1, and, under insulin stimulation, Glut-4 protein mass level. FO normalized insulin resistance and increased n−3 PUFAs in muscle phospholipids.
Fil: D'alessandro, Maria Eugenia Guadalupe. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe; Argentina
Fil: Chicco, Adriana Graciela. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe; Argentina
Fil: Lombardo, Yolanda B.. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Materia
Insulin Action
Dyslipidemia
Polyunsaturated Fatty Acids
Skeletal Muscle
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/6378
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Fish oil reverses the altered glucose transporter, phosphorylation, insulin receptor substrate-1 (IRS-1) protein level and lipid contents in skeletal muscle of sucrose-rich diet fed ratsD'alessandro, Maria Eugenia GuadalupeChicco, Adriana GracielaLombardo, Yolanda B.Insulin ActionDyslipidemiaPolyunsaturated Fatty AcidsSkeletal Musclehttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3The role and underlying mechanisms by which n−3 polyunsaturated fatty acids (PUFA) prevent/reverse SRD-induced insulin resistance (IR) in the muscle are not completely understood. Therefore, we examined: triglyceride, diacylglycerol, PKCθ, Glut-4, enzymatic hexokinase activity, IRS-1 protein mass level, and fatty acid composition of muscle phospholipids. Rats were fed a SRD during 6 months. Thereafter, half the animals continued with SRD up to 8 months; the other half was fed a SRD in which CO (8% wt/wt) was replaced by FO (7%+1% CO) for 2 months. Results were compared with those obtained in rats fed a control diet (CD). In SRD-fed rats, FO oil normalized/improved lipid storage and PKCθ protein mass level. Effects of insulin were comparable with those of CD-fed rats. FO reversed impaired glucose phosphorylation, IRS-1, and, under insulin stimulation, Glut-4 protein mass level. FO normalized insulin resistance and increased n−3 PUFAs in muscle phospholipids.Fil: D'alessandro, Maria Eugenia Guadalupe. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe; ArgentinaFil: Chicco, Adriana Graciela. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe; ArgentinaFil: Lombardo, Yolanda B.. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaElsevier2013-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/6378D'alessandro, Maria Eugenia Guadalupe; Chicco, Adriana Graciela; Lombardo, Yolanda B.; Fish oil reverses the altered glucose transporter, phosphorylation, insulin receptor substrate-1 (IRS-1) protein level and lipid contents in skeletal muscle of sucrose-rich diet fed rats; Elsevier; Prostaglandins Leukotrienes and Essential Fatty Acids; 88; 2; 1-2013; 171-1770952-3278enginfo:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/doi/10.1016/j.plefa.2012.11.003info:eu-repo/semantics/altIdentifier/purl/http://www.sciencedirect.com/science/article/pii/S0952327812001871info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T09:45:21Zoai:ri.conicet.gov.ar:11336/6378instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 09:45:21.64CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Fish oil reverses the altered glucose transporter, phosphorylation, insulin receptor substrate-1 (IRS-1) protein level and lipid contents in skeletal muscle of sucrose-rich diet fed rats
title Fish oil reverses the altered glucose transporter, phosphorylation, insulin receptor substrate-1 (IRS-1) protein level and lipid contents in skeletal muscle of sucrose-rich diet fed rats
spellingShingle Fish oil reverses the altered glucose transporter, phosphorylation, insulin receptor substrate-1 (IRS-1) protein level and lipid contents in skeletal muscle of sucrose-rich diet fed rats
D'alessandro, Maria Eugenia Guadalupe
Insulin Action
Dyslipidemia
Polyunsaturated Fatty Acids
Skeletal Muscle
title_short Fish oil reverses the altered glucose transporter, phosphorylation, insulin receptor substrate-1 (IRS-1) protein level and lipid contents in skeletal muscle of sucrose-rich diet fed rats
title_full Fish oil reverses the altered glucose transporter, phosphorylation, insulin receptor substrate-1 (IRS-1) protein level and lipid contents in skeletal muscle of sucrose-rich diet fed rats
title_fullStr Fish oil reverses the altered glucose transporter, phosphorylation, insulin receptor substrate-1 (IRS-1) protein level and lipid contents in skeletal muscle of sucrose-rich diet fed rats
title_full_unstemmed Fish oil reverses the altered glucose transporter, phosphorylation, insulin receptor substrate-1 (IRS-1) protein level and lipid contents in skeletal muscle of sucrose-rich diet fed rats
title_sort Fish oil reverses the altered glucose transporter, phosphorylation, insulin receptor substrate-1 (IRS-1) protein level and lipid contents in skeletal muscle of sucrose-rich diet fed rats
dc.creator.none.fl_str_mv D'alessandro, Maria Eugenia Guadalupe
Chicco, Adriana Graciela
Lombardo, Yolanda B.
author D'alessandro, Maria Eugenia Guadalupe
author_facet D'alessandro, Maria Eugenia Guadalupe
Chicco, Adriana Graciela
Lombardo, Yolanda B.
author_role author
author2 Chicco, Adriana Graciela
Lombardo, Yolanda B.
author2_role author
author
dc.subject.none.fl_str_mv Insulin Action
Dyslipidemia
Polyunsaturated Fatty Acids
Skeletal Muscle
topic Insulin Action
Dyslipidemia
Polyunsaturated Fatty Acids
Skeletal Muscle
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The role and underlying mechanisms by which n−3 polyunsaturated fatty acids (PUFA) prevent/reverse SRD-induced insulin resistance (IR) in the muscle are not completely understood. Therefore, we examined: triglyceride, diacylglycerol, PKCθ, Glut-4, enzymatic hexokinase activity, IRS-1 protein mass level, and fatty acid composition of muscle phospholipids. Rats were fed a SRD during 6 months. Thereafter, half the animals continued with SRD up to 8 months; the other half was fed a SRD in which CO (8% wt/wt) was replaced by FO (7%+1% CO) for 2 months. Results were compared with those obtained in rats fed a control diet (CD). In SRD-fed rats, FO oil normalized/improved lipid storage and PKCθ protein mass level. Effects of insulin were comparable with those of CD-fed rats. FO reversed impaired glucose phosphorylation, IRS-1, and, under insulin stimulation, Glut-4 protein mass level. FO normalized insulin resistance and increased n−3 PUFAs in muscle phospholipids.
Fil: D'alessandro, Maria Eugenia Guadalupe. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe; Argentina
Fil: Chicco, Adriana Graciela. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe; Argentina
Fil: Lombardo, Yolanda B.. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
description The role and underlying mechanisms by which n−3 polyunsaturated fatty acids (PUFA) prevent/reverse SRD-induced insulin resistance (IR) in the muscle are not completely understood. Therefore, we examined: triglyceride, diacylglycerol, PKCθ, Glut-4, enzymatic hexokinase activity, IRS-1 protein mass level, and fatty acid composition of muscle phospholipids. Rats were fed a SRD during 6 months. Thereafter, half the animals continued with SRD up to 8 months; the other half was fed a SRD in which CO (8% wt/wt) was replaced by FO (7%+1% CO) for 2 months. Results were compared with those obtained in rats fed a control diet (CD). In SRD-fed rats, FO oil normalized/improved lipid storage and PKCθ protein mass level. Effects of insulin were comparable with those of CD-fed rats. FO reversed impaired glucose phosphorylation, IRS-1, and, under insulin stimulation, Glut-4 protein mass level. FO normalized insulin resistance and increased n−3 PUFAs in muscle phospholipids.
publishDate 2013
dc.date.none.fl_str_mv 2013-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/6378
D'alessandro, Maria Eugenia Guadalupe; Chicco, Adriana Graciela; Lombardo, Yolanda B.; Fish oil reverses the altered glucose transporter, phosphorylation, insulin receptor substrate-1 (IRS-1) protein level and lipid contents in skeletal muscle of sucrose-rich diet fed rats; Elsevier; Prostaglandins Leukotrienes and Essential Fatty Acids; 88; 2; 1-2013; 171-177
0952-3278
url http://hdl.handle.net/11336/6378
identifier_str_mv D'alessandro, Maria Eugenia Guadalupe; Chicco, Adriana Graciela; Lombardo, Yolanda B.; Fish oil reverses the altered glucose transporter, phosphorylation, insulin receptor substrate-1 (IRS-1) protein level and lipid contents in skeletal muscle of sucrose-rich diet fed rats; Elsevier; Prostaglandins Leukotrienes and Essential Fatty Acids; 88; 2; 1-2013; 171-177
0952-3278
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.plefa.2012.11.003
info:eu-repo/semantics/altIdentifier/purl/http://www.sciencedirect.com/science/article/pii/S0952327812001871
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1848597821833347072
score 12.976206

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