Plant extracellular vesicles are incorporated by a fungal pathogen and inhibit its growth

Autores
Regente, Mariana Clelia; Pinedo, Marcela Lilian; Clemente, Hélène San; Balliau, Thierry; Jamet, Elisabeth; de la Canal, Laura
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Extracellular vesicles (EV) are membrane particles released by cells into their environment and are considered to be key players in intercellular communication. EV are produced by all domains of life but limited knowledge about EV in plants is available, although their implication in plant defense has been suggested. We have characterized sunflower EV and tested whether they could interact with fungal cells. EV were isolated from extracellular fluids of seedlings and characterized by transmission electron microscopy and proteomic analysis. These nanovesicles appeared to be enriched in cell wall remodeling enzymes and defense proteins. Membrane-labeled EV were prepared and their uptake by the phytopathogenic fungus Sclerotinia sclerotiorum was verifed. Functional tests further evaluated the ability of EV to affect fungal growth. Spores treated with plant EV showed growth inhibition, morphological changes, and cell death. Conclusive evidence on the existence of plant EV is presented and we demonstrate their ability to interact with and kill fungal cells. Our results introduce the concept of cell-to-cell communication through EV in plants.
Fil: Regente, Mariana Clelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Pinedo, Marcela Lilian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
Fil: Clemente, Hélène San. Universite Toulose 1 Capitole; Francia
Fil: Balliau, Thierry. Université de Paris XI; Francia
Fil: Jamet, Elisabeth. Universite Toulose 1 Capitole; Francia
Fil: de la Canal, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Materia
Antifungal
Apoplast
Exosomes
Extracellular Vesicles
Fungal Growth
Intercellular Communication
Plant Defense
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/65508
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Plant extracellular vesicles are incorporated by a fungal pathogen and inhibit its growthRegente, Mariana CleliaPinedo, Marcela LilianClemente, Hélène SanBalliau, ThierryJamet, Elisabethde la Canal, LauraAntifungalApoplastExosomesExtracellular VesiclesFungal GrowthIntercellular CommunicationPlant Defensehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Extracellular vesicles (EV) are membrane particles released by cells into their environment and are considered to be key players in intercellular communication. EV are produced by all domains of life but limited knowledge about EV in plants is available, although their implication in plant defense has been suggested. We have characterized sunflower EV and tested whether they could interact with fungal cells. EV were isolated from extracellular fluids of seedlings and characterized by transmission electron microscopy and proteomic analysis. These nanovesicles appeared to be enriched in cell wall remodeling enzymes and defense proteins. Membrane-labeled EV were prepared and their uptake by the phytopathogenic fungus Sclerotinia sclerotiorum was verifed. Functional tests further evaluated the ability of EV to affect fungal growth. Spores treated with plant EV showed growth inhibition, morphological changes, and cell death. Conclusive evidence on the existence of plant EV is presented and we demonstrate their ability to interact with and kill fungal cells. Our results introduce the concept of cell-to-cell communication through EV in plants.Fil: Regente, Mariana Clelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Pinedo, Marcela Lilian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; ArgentinaFil: Clemente, Hélène San. Universite Toulose 1 Capitole; FranciaFil: Balliau, Thierry. Université de Paris XI; FranciaFil: Jamet, Elisabeth. Universite Toulose 1 Capitole; FranciaFil: de la Canal, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaOxford University Press2017-09-23info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/65508Regente, Mariana Clelia; Pinedo, Marcela Lilian; Clemente, Hélène San; Balliau, Thierry; Jamet, Elisabeth; et al.; Plant extracellular vesicles are incorporated by a fungal pathogen and inhibit its growth; Oxford University Press; Journal of Experimental Botany; 68; 20; 23-9-2017; 5485-54950022-0957CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1093/jxb/erx355info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jxb/article/68/20/5485/4626762info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:45:09Zoai:ri.conicet.gov.ar:11336/65508instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:45:09.348CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Plant extracellular vesicles are incorporated by a fungal pathogen and inhibit its growth
title Plant extracellular vesicles are incorporated by a fungal pathogen and inhibit its growth
spellingShingle Plant extracellular vesicles are incorporated by a fungal pathogen and inhibit its growth
Regente, Mariana Clelia
Antifungal
Apoplast
Exosomes
Extracellular Vesicles
Fungal Growth
Intercellular Communication
Plant Defense
title_short Plant extracellular vesicles are incorporated by a fungal pathogen and inhibit its growth
title_full Plant extracellular vesicles are incorporated by a fungal pathogen and inhibit its growth
title_fullStr Plant extracellular vesicles are incorporated by a fungal pathogen and inhibit its growth
title_full_unstemmed Plant extracellular vesicles are incorporated by a fungal pathogen and inhibit its growth
title_sort Plant extracellular vesicles are incorporated by a fungal pathogen and inhibit its growth
dc.creator.none.fl_str_mv Regente, Mariana Clelia
Pinedo, Marcela Lilian
Clemente, Hélène San
Balliau, Thierry
Jamet, Elisabeth
de la Canal, Laura
author Regente, Mariana Clelia
author_facet Regente, Mariana Clelia
Pinedo, Marcela Lilian
Clemente, Hélène San
Balliau, Thierry
Jamet, Elisabeth
de la Canal, Laura
author_role author
author2 Pinedo, Marcela Lilian
Clemente, Hélène San
Balliau, Thierry
Jamet, Elisabeth
de la Canal, Laura
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Antifungal
Apoplast
Exosomes
Extracellular Vesicles
Fungal Growth
Intercellular Communication
Plant Defense
topic Antifungal
Apoplast
Exosomes
Extracellular Vesicles
Fungal Growth
Intercellular Communication
Plant Defense
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Extracellular vesicles (EV) are membrane particles released by cells into their environment and are considered to be key players in intercellular communication. EV are produced by all domains of life but limited knowledge about EV in plants is available, although their implication in plant defense has been suggested. We have characterized sunflower EV and tested whether they could interact with fungal cells. EV were isolated from extracellular fluids of seedlings and characterized by transmission electron microscopy and proteomic analysis. These nanovesicles appeared to be enriched in cell wall remodeling enzymes and defense proteins. Membrane-labeled EV were prepared and their uptake by the phytopathogenic fungus Sclerotinia sclerotiorum was verifed. Functional tests further evaluated the ability of EV to affect fungal growth. Spores treated with plant EV showed growth inhibition, morphological changes, and cell death. Conclusive evidence on the existence of plant EV is presented and we demonstrate their ability to interact with and kill fungal cells. Our results introduce the concept of cell-to-cell communication through EV in plants.
Fil: Regente, Mariana Clelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Pinedo, Marcela Lilian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
Fil: Clemente, Hélène San. Universite Toulose 1 Capitole; Francia
Fil: Balliau, Thierry. Université de Paris XI; Francia
Fil: Jamet, Elisabeth. Universite Toulose 1 Capitole; Francia
Fil: de la Canal, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
description Extracellular vesicles (EV) are membrane particles released by cells into their environment and are considered to be key players in intercellular communication. EV are produced by all domains of life but limited knowledge about EV in plants is available, although their implication in plant defense has been suggested. We have characterized sunflower EV and tested whether they could interact with fungal cells. EV were isolated from extracellular fluids of seedlings and characterized by transmission electron microscopy and proteomic analysis. These nanovesicles appeared to be enriched in cell wall remodeling enzymes and defense proteins. Membrane-labeled EV were prepared and their uptake by the phytopathogenic fungus Sclerotinia sclerotiorum was verifed. Functional tests further evaluated the ability of EV to affect fungal growth. Spores treated with plant EV showed growth inhibition, morphological changes, and cell death. Conclusive evidence on the existence of plant EV is presented and we demonstrate their ability to interact with and kill fungal cells. Our results introduce the concept of cell-to-cell communication through EV in plants.
publishDate 2017
dc.date.none.fl_str_mv 2017-09-23
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/65508
Regente, Mariana Clelia; Pinedo, Marcela Lilian; Clemente, Hélène San; Balliau, Thierry; Jamet, Elisabeth; et al.; Plant extracellular vesicles are incorporated by a fungal pathogen and inhibit its growth; Oxford University Press; Journal of Experimental Botany; 68; 20; 23-9-2017; 5485-5495
0022-0957
CONICET Digital
CONICET
url http://hdl.handle.net/11336/65508
identifier_str_mv Regente, Mariana Clelia; Pinedo, Marcela Lilian; Clemente, Hélène San; Balliau, Thierry; Jamet, Elisabeth; et al.; Plant extracellular vesicles are incorporated by a fungal pathogen and inhibit its growth; Oxford University Press; Journal of Experimental Botany; 68; 20; 23-9-2017; 5485-5495
0022-0957
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1093/jxb/erx355
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jxb/article/68/20/5485/4626762
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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