The protein and microRNA cargo of extracellular vesicles from parasitic helminths – current status and research priorities

Autores
Sotillo, Javier; Robinson, Mark W.; Kimber, Michael J.; Cucher, Marcela Alejandra; Ancarola, María Eugenia; Nejsum, Peter; Marcilla, Antonio; Eichenberger, Ramon M.; Tritten, Lucienne
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Helminth parasites have a remarkable ability to persist within their mammalian hosts, which is largely due to their secretion of molecules with immunomodulatory properties. Although the soluble components of helminth secretions have been extensively studied, the discovery that helminths release extracellular vesicles (EVs) has added further complexity to the host-parasite interaction. Whilst several studies have begun to characterise the molecules carried by helminth EVs, work aimed at investigating their biological functions has been hindered by a lack of helminth-specific EV markers. To begin to address this, we summarised helminth EV literature to date. With a focus on the protein and microRNA (miRNA) cargo, we aimed to detect similarities and differences across those major groups of helminths for which data are available; namely nematodes, trematodes and cestodes. Pfam analysis revealed that although there is no universal EV marker for all helminth species, the EF-hand protein family was present in all EV datasets from cestodes and trematodes, and could serve as a platyhelminth EV biomarker. In contrast, M13 metallopeptidases and actin may have potential as markers for nematode EVs. As with proteins, many miRNA families appeared to be species-, stage-, or dataset-specific. Two miRNA families were common to nematode EVs (mir-10 and let-7); the miRNA cargo of EVs secreted by clade I species appeared somewhat different from species from other clades. Five miRNA families (mir-71, mir-10, mir-190, let-7 and mir-2) were shared by all trematode species examined. Our analysis has identified novel markers that may be used in studies aimed at characterising helminth EVs and interrogating their function at the host-parasite interface. In addition, we discuss the heterogeneity of methods used for helminth EV isolation and emphasise the need for a standardised approach in reporting on helminth EV data.
Fil: Sotillo, Javier. Instituto de Salud Carlos Iii (isciii); España
Fil: Robinson, Mark W.. The Queens University of Belfast; Irlanda
Fil: Kimber, Michael J.. Queen’s University ; Reino Unido
Fil: Cucher, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Ancarola, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Nejsum, Peter. Aarhus University. Aarhus Institute Of Advanced Studies.; Dinamarca
Fil: Marcilla, Antonio. Universidad de Valencia; España
Fil: Eichenberger, Ramon M.. Universitat Zurich; Suiza
Fil: Tritten, Lucienne. Universitat Zurich; Suiza
Materia
CARGO
EXOSOMES
EXTRACELLULAR VESICLES
HELMINTHS
MICRORNA
MICROVESICLES
PARASITES
PROTEIN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/142812
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/142812
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The protein and microRNA cargo of extracellular vesicles from parasitic helminths – current status and research prioritiesSotillo, JavierRobinson, Mark W.Kimber, Michael J.Cucher, Marcela AlejandraAncarola, María EugeniaNejsum, PeterMarcilla, AntonioEichenberger, Ramon M.Tritten, LucienneCARGOEXOSOMESEXTRACELLULAR VESICLESHELMINTHSMICRORNAMICROVESICLESPARASITESPROTEINhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Helminth parasites have a remarkable ability to persist within their mammalian hosts, which is largely due to their secretion of molecules with immunomodulatory properties. Although the soluble components of helminth secretions have been extensively studied, the discovery that helminths release extracellular vesicles (EVs) has added further complexity to the host-parasite interaction. Whilst several studies have begun to characterise the molecules carried by helminth EVs, work aimed at investigating their biological functions has been hindered by a lack of helminth-specific EV markers. To begin to address this, we summarised helminth EV literature to date. With a focus on the protein and microRNA (miRNA) cargo, we aimed to detect similarities and differences across those major groups of helminths for which data are available; namely nematodes, trematodes and cestodes. Pfam analysis revealed that although there is no universal EV marker for all helminth species, the EF-hand protein family was present in all EV datasets from cestodes and trematodes, and could serve as a platyhelminth EV biomarker. In contrast, M13 metallopeptidases and actin may have potential as markers for nematode EVs. As with proteins, many miRNA families appeared to be species-, stage-, or dataset-specific. Two miRNA families were common to nematode EVs (mir-10 and let-7); the miRNA cargo of EVs secreted by clade I species appeared somewhat different from species from other clades. Five miRNA families (mir-71, mir-10, mir-190, let-7 and mir-2) were shared by all trematode species examined. Our analysis has identified novel markers that may be used in studies aimed at characterising helminth EVs and interrogating their function at the host-parasite interface. In addition, we discuss the heterogeneity of methods used for helminth EV isolation and emphasise the need for a standardised approach in reporting on helminth EV data.Fil: Sotillo, Javier. Instituto de Salud Carlos Iii (isciii); EspañaFil: Robinson, Mark W.. The Queens University of Belfast; IrlandaFil: Kimber, Michael J.. Queen’s University ; Reino UnidoFil: Cucher, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Ancarola, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Nejsum, Peter. Aarhus University. Aarhus Institute Of Advanced Studies.; DinamarcaFil: Marcilla, Antonio. Universidad de Valencia; EspañaFil: Eichenberger, Ramon M.. Universitat Zurich; SuizaFil: Tritten, Lucienne. Universitat Zurich; SuizaElsevier2020-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/142812Sotillo, Javier; Robinson, Mark W.; Kimber, Michael J.; Cucher, Marcela Alejandra; Ancarola, María Eugenia; et al.; The protein and microRNA cargo of extracellular vesicles from parasitic helminths – current status and research priorities; Elsevier; International Journal for Parasitology; 50; 9; 8-2020; 635-6450020-7519CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijpara.2020.04.010info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0020751920301612?via%3Dihubinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:31Zoai:ri.conicet.gov.ar:11336/142812instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:31.685CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The protein and microRNA cargo of extracellular vesicles from parasitic helminths – current status and research priorities
title The protein and microRNA cargo of extracellular vesicles from parasitic helminths – current status and research priorities
spellingShingle The protein and microRNA cargo of extracellular vesicles from parasitic helminths – current status and research priorities
Sotillo, Javier
CARGO
EXOSOMES
EXTRACELLULAR VESICLES
HELMINTHS
MICRORNA
MICROVESICLES
PARASITES
PROTEIN
title_short The protein and microRNA cargo of extracellular vesicles from parasitic helminths – current status and research priorities
title_full The protein and microRNA cargo of extracellular vesicles from parasitic helminths – current status and research priorities
title_fullStr The protein and microRNA cargo of extracellular vesicles from parasitic helminths – current status and research priorities
title_full_unstemmed The protein and microRNA cargo of extracellular vesicles from parasitic helminths – current status and research priorities
title_sort The protein and microRNA cargo of extracellular vesicles from parasitic helminths – current status and research priorities
dc.creator.none.fl_str_mv Sotillo, Javier
Robinson, Mark W.
Kimber, Michael J.
Cucher, Marcela Alejandra
Ancarola, María Eugenia
Nejsum, Peter
Marcilla, Antonio
Eichenberger, Ramon M.
Tritten, Lucienne
author Sotillo, Javier
author_facet Sotillo, Javier
Robinson, Mark W.
Kimber, Michael J.
Cucher, Marcela Alejandra
Ancarola, María Eugenia
Nejsum, Peter
Marcilla, Antonio
Eichenberger, Ramon M.
Tritten, Lucienne
author_role author
author2 Robinson, Mark W.
Kimber, Michael J.
Cucher, Marcela Alejandra
Ancarola, María Eugenia
Nejsum, Peter
Marcilla, Antonio
Eichenberger, Ramon M.
Tritten, Lucienne
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CARGO
EXOSOMES
EXTRACELLULAR VESICLES
HELMINTHS
MICRORNA
MICROVESICLES
PARASITES
PROTEIN
topic CARGO
EXOSOMES
EXTRACELLULAR VESICLES
HELMINTHS
MICRORNA
MICROVESICLES
PARASITES
PROTEIN
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Helminth parasites have a remarkable ability to persist within their mammalian hosts, which is largely due to their secretion of molecules with immunomodulatory properties. Although the soluble components of helminth secretions have been extensively studied, the discovery that helminths release extracellular vesicles (EVs) has added further complexity to the host-parasite interaction. Whilst several studies have begun to characterise the molecules carried by helminth EVs, work aimed at investigating their biological functions has been hindered by a lack of helminth-specific EV markers. To begin to address this, we summarised helminth EV literature to date. With a focus on the protein and microRNA (miRNA) cargo, we aimed to detect similarities and differences across those major groups of helminths for which data are available; namely nematodes, trematodes and cestodes. Pfam analysis revealed that although there is no universal EV marker for all helminth species, the EF-hand protein family was present in all EV datasets from cestodes and trematodes, and could serve as a platyhelminth EV biomarker. In contrast, M13 metallopeptidases and actin may have potential as markers for nematode EVs. As with proteins, many miRNA families appeared to be species-, stage-, or dataset-specific. Two miRNA families were common to nematode EVs (mir-10 and let-7); the miRNA cargo of EVs secreted by clade I species appeared somewhat different from species from other clades. Five miRNA families (mir-71, mir-10, mir-190, let-7 and mir-2) were shared by all trematode species examined. Our analysis has identified novel markers that may be used in studies aimed at characterising helminth EVs and interrogating their function at the host-parasite interface. In addition, we discuss the heterogeneity of methods used for helminth EV isolation and emphasise the need for a standardised approach in reporting on helminth EV data.
Fil: Sotillo, Javier. Instituto de Salud Carlos Iii (isciii); España
Fil: Robinson, Mark W.. The Queens University of Belfast; Irlanda
Fil: Kimber, Michael J.. Queen’s University ; Reino Unido
Fil: Cucher, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Ancarola, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Nejsum, Peter. Aarhus University. Aarhus Institute Of Advanced Studies.; Dinamarca
Fil: Marcilla, Antonio. Universidad de Valencia; España
Fil: Eichenberger, Ramon M.. Universitat Zurich; Suiza
Fil: Tritten, Lucienne. Universitat Zurich; Suiza
description Helminth parasites have a remarkable ability to persist within their mammalian hosts, which is largely due to their secretion of molecules with immunomodulatory properties. Although the soluble components of helminth secretions have been extensively studied, the discovery that helminths release extracellular vesicles (EVs) has added further complexity to the host-parasite interaction. Whilst several studies have begun to characterise the molecules carried by helminth EVs, work aimed at investigating their biological functions has been hindered by a lack of helminth-specific EV markers. To begin to address this, we summarised helminth EV literature to date. With a focus on the protein and microRNA (miRNA) cargo, we aimed to detect similarities and differences across those major groups of helminths for which data are available; namely nematodes, trematodes and cestodes. Pfam analysis revealed that although there is no universal EV marker for all helminth species, the EF-hand protein family was present in all EV datasets from cestodes and trematodes, and could serve as a platyhelminth EV biomarker. In contrast, M13 metallopeptidases and actin may have potential as markers for nematode EVs. As with proteins, many miRNA families appeared to be species-, stage-, or dataset-specific. Two miRNA families were common to nematode EVs (mir-10 and let-7); the miRNA cargo of EVs secreted by clade I species appeared somewhat different from species from other clades. Five miRNA families (mir-71, mir-10, mir-190, let-7 and mir-2) were shared by all trematode species examined. Our analysis has identified novel markers that may be used in studies aimed at characterising helminth EVs and interrogating their function at the host-parasite interface. In addition, we discuss the heterogeneity of methods used for helminth EV isolation and emphasise the need for a standardised approach in reporting on helminth EV data.
publishDate 2020
dc.date.none.fl_str_mv 2020-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/142812
Sotillo, Javier; Robinson, Mark W.; Kimber, Michael J.; Cucher, Marcela Alejandra; Ancarola, María Eugenia; et al.; The protein and microRNA cargo of extracellular vesicles from parasitic helminths – current status and research priorities; Elsevier; International Journal for Parasitology; 50; 9; 8-2020; 635-645
0020-7519
CONICET Digital
CONICET
url http://hdl.handle.net/11336/142812
identifier_str_mv Sotillo, Javier; Robinson, Mark W.; Kimber, Michael J.; Cucher, Marcela Alejandra; Ancarola, María Eugenia; et al.; The protein and microRNA cargo of extracellular vesicles from parasitic helminths – current status and research priorities; Elsevier; International Journal for Parasitology; 50; 9; 8-2020; 635-645
0020-7519
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijpara.2020.04.010
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0020751920301612?via%3Dihub
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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