Characterization of three Ixodes scapularis cDNAs protective against tick infestations

Autores
Almazán, Consuelo; Blas Machado, Uriel; Kocan, Katherine M.; Yoshioka, Joy H.; Blouin, Edmour F.; Mangold, Atilio Jose; de la Fuente, José
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
cDNA expression library immunization (ELI) and analysis of expressed sequenced tags (EST) in a mouse model of tick infestations was used to identified cDNA clones that affected I. scapularis. Three protective antigens against larval tick infestations, 4F8, with homology to a nucleotidase, and 4D8 and 4E6 of unknown function, were selected for further characterization. All three antigens were expressed in all I. scapularis stages and localized in adult tick tissues. 4D8 was shown to be conserved in six other tick species. Based on immunization trials with synthetic polypeptides against larvae and nymphs and on artificial feeding experiments of adults, these antigens, especially 4D8, appear to be good candidates for continued development of a vaccine for control of tick infestations and may be useful in a formulation to target multiple species of ticks.
Fil: Almazán, Consuelo. Oklahoma State University; Estados Unidos
Fil: Blas Machado, Uriel. Oklahoma State University; Estados Unidos
Fil: Kocan, Katherine M.. Oklahoma State University; Estados Unidos
Fil: Yoshioka, Joy H.. Oklahoma State University; Estados Unidos
Fil: Blouin, Edmour F.. Oklahoma State University; Estados Unidos
Fil: Mangold, Atilio Jose. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: de la Fuente, José. Oklahoma State University; Estados Unidos. Universidad de Castilla-La Mancha. Instituto de Investigación en Recursos Cinegéticos; España. Consejo Superior de Investigaciones Científicas; España
Materia
Tick
Recombinant protein
EST
Expression library immunization
Vaccine
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/102580
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characterization of three Ixodes scapularis cDNAs protective against tick infestationsAlmazán, ConsueloBlas Machado, UrielKocan, Katherine M.Yoshioka, Joy H.Blouin, Edmour F.Mangold, Atilio Josede la Fuente, JoséTickRecombinant proteinESTExpression library immunizationVaccinehttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4cDNA expression library immunization (ELI) and analysis of expressed sequenced tags (EST) in a mouse model of tick infestations was used to identified cDNA clones that affected I. scapularis. Three protective antigens against larval tick infestations, 4F8, with homology to a nucleotidase, and 4D8 and 4E6 of unknown function, were selected for further characterization. All three antigens were expressed in all I. scapularis stages and localized in adult tick tissues. 4D8 was shown to be conserved in six other tick species. Based on immunization trials with synthetic polypeptides against larvae and nymphs and on artificial feeding experiments of adults, these antigens, especially 4D8, appear to be good candidates for continued development of a vaccine for control of tick infestations and may be useful in a formulation to target multiple species of ticks.Fil: Almazán, Consuelo. Oklahoma State University; Estados UnidosFil: Blas Machado, Uriel. Oklahoma State University; Estados UnidosFil: Kocan, Katherine M.. Oklahoma State University; Estados UnidosFil: Yoshioka, Joy H.. Oklahoma State University; Estados UnidosFil: Blouin, Edmour F.. Oklahoma State University; Estados UnidosFil: Mangold, Atilio Jose. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: de la Fuente, José. Oklahoma State University; Estados Unidos. Universidad de Castilla-La Mancha. Instituto de Investigación en Recursos Cinegéticos; España. Consejo Superior de Investigaciones Científicas; EspañaElsevier2005-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/102580Almazán, Consuelo; Blas Machado, Uriel; Kocan, Katherine M.; Yoshioka, Joy H.; Blouin, Edmour F.; et al.; Characterization of three Ixodes scapularis cDNAs protective against tick infestations; Elsevier; Vaccine; 23; 35; 8-2005; 4403-44160264-410XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.vaccine.2005.04.012info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0264410X05004408info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:32:27Zoai:ri.conicet.gov.ar:11336/102580instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:32:28.035CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization of three Ixodes scapularis cDNAs protective against tick infestations
title Characterization of three Ixodes scapularis cDNAs protective against tick infestations
spellingShingle Characterization of three Ixodes scapularis cDNAs protective against tick infestations
Almazán, Consuelo
Tick
Recombinant protein
EST
Expression library immunization
Vaccine
title_short Characterization of three Ixodes scapularis cDNAs protective against tick infestations
title_full Characterization of three Ixodes scapularis cDNAs protective against tick infestations
title_fullStr Characterization of three Ixodes scapularis cDNAs protective against tick infestations
title_full_unstemmed Characterization of three Ixodes scapularis cDNAs protective against tick infestations
title_sort Characterization of three Ixodes scapularis cDNAs protective against tick infestations
dc.creator.none.fl_str_mv Almazán, Consuelo
Blas Machado, Uriel
Kocan, Katherine M.
Yoshioka, Joy H.
Blouin, Edmour F.
Mangold, Atilio Jose
de la Fuente, José
author Almazán, Consuelo
author_facet Almazán, Consuelo
Blas Machado, Uriel
Kocan, Katherine M.
Yoshioka, Joy H.
Blouin, Edmour F.
Mangold, Atilio Jose
de la Fuente, José
author_role author
author2 Blas Machado, Uriel
Kocan, Katherine M.
Yoshioka, Joy H.
Blouin, Edmour F.
Mangold, Atilio Jose
de la Fuente, José
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Tick
Recombinant protein
EST
Expression library immunization
Vaccine
topic Tick
Recombinant protein
EST
Expression library immunization
Vaccine
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv cDNA expression library immunization (ELI) and analysis of expressed sequenced tags (EST) in a mouse model of tick infestations was used to identified cDNA clones that affected I. scapularis. Three protective antigens against larval tick infestations, 4F8, with homology to a nucleotidase, and 4D8 and 4E6 of unknown function, were selected for further characterization. All three antigens were expressed in all I. scapularis stages and localized in adult tick tissues. 4D8 was shown to be conserved in six other tick species. Based on immunization trials with synthetic polypeptides against larvae and nymphs and on artificial feeding experiments of adults, these antigens, especially 4D8, appear to be good candidates for continued development of a vaccine for control of tick infestations and may be useful in a formulation to target multiple species of ticks.
Fil: Almazán, Consuelo. Oklahoma State University; Estados Unidos
Fil: Blas Machado, Uriel. Oklahoma State University; Estados Unidos
Fil: Kocan, Katherine M.. Oklahoma State University; Estados Unidos
Fil: Yoshioka, Joy H.. Oklahoma State University; Estados Unidos
Fil: Blouin, Edmour F.. Oklahoma State University; Estados Unidos
Fil: Mangold, Atilio Jose. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: de la Fuente, José. Oklahoma State University; Estados Unidos. Universidad de Castilla-La Mancha. Instituto de Investigación en Recursos Cinegéticos; España. Consejo Superior de Investigaciones Científicas; España
description cDNA expression library immunization (ELI) and analysis of expressed sequenced tags (EST) in a mouse model of tick infestations was used to identified cDNA clones that affected I. scapularis. Three protective antigens against larval tick infestations, 4F8, with homology to a nucleotidase, and 4D8 and 4E6 of unknown function, were selected for further characterization. All three antigens were expressed in all I. scapularis stages and localized in adult tick tissues. 4D8 was shown to be conserved in six other tick species. Based on immunization trials with synthetic polypeptides against larvae and nymphs and on artificial feeding experiments of adults, these antigens, especially 4D8, appear to be good candidates for continued development of a vaccine for control of tick infestations and may be useful in a formulation to target multiple species of ticks.
publishDate 2005
dc.date.none.fl_str_mv 2005-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/102580
Almazán, Consuelo; Blas Machado, Uriel; Kocan, Katherine M.; Yoshioka, Joy H.; Blouin, Edmour F.; et al.; Characterization of three Ixodes scapularis cDNAs protective against tick infestations; Elsevier; Vaccine; 23; 35; 8-2005; 4403-4416
0264-410X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/102580
identifier_str_mv Almazán, Consuelo; Blas Machado, Uriel; Kocan, Katherine M.; Yoshioka, Joy H.; Blouin, Edmour F.; et al.; Characterization of three Ixodes scapularis cDNAs protective against tick infestations; Elsevier; Vaccine; 23; 35; 8-2005; 4403-4416
0264-410X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vaccine.2005.04.012
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0264410X05004408
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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