Evidence of association of a BTA20 region peaked in ISL1 with puberty in Angus bulls

Autores
Fernandez, María Elena; Liron, Juan Pedro; Prando, Alberto; Rogberg Muñoz, Andres; Peral Garcia, Pilar; Baldo A; Giovambattista, Andres
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In bovine, there are significant differences among and within breeds in the time when bulls reach puberty. Reported data indicate that age at puberty has a strong genetic component and is a multigenic trait. However, despite significant advancements in the field, the gene network controlling puberty is very complex, containing a host of genes that exert their effect on the hypothalamic–pituitary–gonadal (HPG) axis, and several aspects of regulation of the HPG axis are yet to be fully elucidated. The objective of this work was to identify new genes associated with age at puberty. Angus males (N=276) were weighted and scrotal circumference measured every month. When first bull calves reached 26 cm of scrotal circumference, sperm quality was added to the monthly measurements for the next three months. Based on sperm quality, two groups corresponding to the top and bottom 6.5% of the phenotypic distribution curve were selected to perform a genome-wide association study using a selective DNA approach, to be used as an exploratory analysis. Based on the results of this preliminary study, five SNPs located within BTA3, 20 and 24 associated regions were genotyped in the 276 bulls using pyrosequencing methods. This analysis allowed us to confirm the association of a BTA20 region with age at puberty estimated at C 50 million. The associated SNP explained 1.69% of the genetic variance of age at puberty at C 50 million. The associated region contained within and nearby positional candidate genes (ISL1, PELO, FST, SPZ1) that may be involved in spermatogenesis. Our results should encourage further investigation to confirm the role of these genes in order to increase our knowledge on genetic control of bovine puberty.
Fil: Fernandez, María Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Genética Veterinaria "ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Liron, Juan Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Genética Veterinaria "ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Prando, Alberto. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Rogberg Muñoz, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Genética Veterinaria "ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Peral Garcia, Pilar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Genética Veterinaria "ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Baldo A. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Giovambattista, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Genética Veterinaria "ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Materia
Puberty
Beef Cattle
Genome Wide Association
Selective Dna Approach
Single Nucleotide Polymorphisms
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/11595
Acceder
id CONICETDig_960db737de57058c1b1da3b9ee217355
oai_identifier_str oai:ri.conicet.gov.ar:11336/11595
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evidence of association of a BTA20 region peaked in ISL1 with puberty in Angus bullsFernandez, María ElenaLiron, Juan PedroPrando, AlbertoRogberg Muñoz, AndresPeral Garcia, PilarBaldo AGiovambattista, AndresPubertyBeef CattleGenome Wide AssociationSelective Dna ApproachSingle Nucleotide Polymorphismshttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4In bovine, there are significant differences among and within breeds in the time when bulls reach puberty. Reported data indicate that age at puberty has a strong genetic component and is a multigenic trait. However, despite significant advancements in the field, the gene network controlling puberty is very complex, containing a host of genes that exert their effect on the hypothalamic–pituitary–gonadal (HPG) axis, and several aspects of regulation of the HPG axis are yet to be fully elucidated. The objective of this work was to identify new genes associated with age at puberty. Angus males (N=276) were weighted and scrotal circumference measured every month. When first bull calves reached 26 cm of scrotal circumference, sperm quality was added to the monthly measurements for the next three months. Based on sperm quality, two groups corresponding to the top and bottom 6.5% of the phenotypic distribution curve were selected to perform a genome-wide association study using a selective DNA approach, to be used as an exploratory analysis. Based on the results of this preliminary study, five SNPs located within BTA3, 20 and 24 associated regions were genotyped in the 276 bulls using pyrosequencing methods. This analysis allowed us to confirm the association of a BTA20 region with age at puberty estimated at C 50 million. The associated SNP explained 1.69% of the genetic variance of age at puberty at C 50 million. The associated region contained within and nearby positional candidate genes (ISL1, PELO, FST, SPZ1) that may be involved in spermatogenesis. Our results should encourage further investigation to confirm the role of these genes in order to increase our knowledge on genetic control of bovine puberty.Fil: Fernandez, María Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Genética Veterinaria "ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Liron, Juan Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Genética Veterinaria "ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Prando, Alberto. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Rogberg Muñoz, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Genética Veterinaria "ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Peral Garcia, Pilar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Genética Veterinaria "ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Baldo A. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Giovambattista, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Genética Veterinaria "ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaElsevier Science2014-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/11595Fernandez, María Elena; Liron, Juan Pedro; Prando, Alberto; Rogberg Muñoz, Andres; Peral Garcia, Pilar; et al.; Evidence of association of a BTA20 region peaked in ISL1 with puberty in Angus bulls; Elsevier Science; Livestock Science; 167; 9-2014; 9-181871-1413enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.livsci.2014.05.009info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1871141314002455info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:53:40Zoai:ri.conicet.gov.ar:11336/11595instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:53:40.833CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evidence of association of a BTA20 region peaked in ISL1 with puberty in Angus bulls
title Evidence of association of a BTA20 region peaked in ISL1 with puberty in Angus bulls
spellingShingle Evidence of association of a BTA20 region peaked in ISL1 with puberty in Angus bulls
Fernandez, María Elena
Puberty
Beef Cattle
Genome Wide Association
Selective Dna Approach
Single Nucleotide Polymorphisms
title_short Evidence of association of a BTA20 region peaked in ISL1 with puberty in Angus bulls
title_full Evidence of association of a BTA20 region peaked in ISL1 with puberty in Angus bulls
title_fullStr Evidence of association of a BTA20 region peaked in ISL1 with puberty in Angus bulls
title_full_unstemmed Evidence of association of a BTA20 region peaked in ISL1 with puberty in Angus bulls
title_sort Evidence of association of a BTA20 region peaked in ISL1 with puberty in Angus bulls
dc.creator.none.fl_str_mv Fernandez, María Elena
Liron, Juan Pedro
Prando, Alberto
Rogberg Muñoz, Andres
Peral Garcia, Pilar
Baldo A
Giovambattista, Andres
author Fernandez, María Elena
author_facet Fernandez, María Elena
Liron, Juan Pedro
Prando, Alberto
Rogberg Muñoz, Andres
Peral Garcia, Pilar
Baldo A
Giovambattista, Andres
author_role author
author2 Liron, Juan Pedro
Prando, Alberto
Rogberg Muñoz, Andres
Peral Garcia, Pilar
Baldo A
Giovambattista, Andres
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Puberty
Beef Cattle
Genome Wide Association
Selective Dna Approach
Single Nucleotide Polymorphisms
topic Puberty
Beef Cattle
Genome Wide Association
Selective Dna Approach
Single Nucleotide Polymorphisms
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv In bovine, there are significant differences among and within breeds in the time when bulls reach puberty. Reported data indicate that age at puberty has a strong genetic component and is a multigenic trait. However, despite significant advancements in the field, the gene network controlling puberty is very complex, containing a host of genes that exert their effect on the hypothalamic–pituitary–gonadal (HPG) axis, and several aspects of regulation of the HPG axis are yet to be fully elucidated. The objective of this work was to identify new genes associated with age at puberty. Angus males (N=276) were weighted and scrotal circumference measured every month. When first bull calves reached 26 cm of scrotal circumference, sperm quality was added to the monthly measurements for the next three months. Based on sperm quality, two groups corresponding to the top and bottom 6.5% of the phenotypic distribution curve were selected to perform a genome-wide association study using a selective DNA approach, to be used as an exploratory analysis. Based on the results of this preliminary study, five SNPs located within BTA3, 20 and 24 associated regions were genotyped in the 276 bulls using pyrosequencing methods. This analysis allowed us to confirm the association of a BTA20 region with age at puberty estimated at C 50 million. The associated SNP explained 1.69% of the genetic variance of age at puberty at C 50 million. The associated region contained within and nearby positional candidate genes (ISL1, PELO, FST, SPZ1) that may be involved in spermatogenesis. Our results should encourage further investigation to confirm the role of these genes in order to increase our knowledge on genetic control of bovine puberty.
Fil: Fernandez, María Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Genética Veterinaria "ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Liron, Juan Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Genética Veterinaria "ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Prando, Alberto. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Rogberg Muñoz, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Genética Veterinaria "ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Peral Garcia, Pilar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Genética Veterinaria "ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Baldo A. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Giovambattista, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Genética Veterinaria "ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
description In bovine, there are significant differences among and within breeds in the time when bulls reach puberty. Reported data indicate that age at puberty has a strong genetic component and is a multigenic trait. However, despite significant advancements in the field, the gene network controlling puberty is very complex, containing a host of genes that exert their effect on the hypothalamic–pituitary–gonadal (HPG) axis, and several aspects of regulation of the HPG axis are yet to be fully elucidated. The objective of this work was to identify new genes associated with age at puberty. Angus males (N=276) were weighted and scrotal circumference measured every month. When first bull calves reached 26 cm of scrotal circumference, sperm quality was added to the monthly measurements for the next three months. Based on sperm quality, two groups corresponding to the top and bottom 6.5% of the phenotypic distribution curve were selected to perform a genome-wide association study using a selective DNA approach, to be used as an exploratory analysis. Based on the results of this preliminary study, five SNPs located within BTA3, 20 and 24 associated regions were genotyped in the 276 bulls using pyrosequencing methods. This analysis allowed us to confirm the association of a BTA20 region with age at puberty estimated at C 50 million. The associated SNP explained 1.69% of the genetic variance of age at puberty at C 50 million. The associated region contained within and nearby positional candidate genes (ISL1, PELO, FST, SPZ1) that may be involved in spermatogenesis. Our results should encourage further investigation to confirm the role of these genes in order to increase our knowledge on genetic control of bovine puberty.
publishDate 2014
dc.date.none.fl_str_mv 2014-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/11595
Fernandez, María Elena; Liron, Juan Pedro; Prando, Alberto; Rogberg Muñoz, Andres; Peral Garcia, Pilar; et al.; Evidence of association of a BTA20 region peaked in ISL1 with puberty in Angus bulls; Elsevier Science; Livestock Science; 167; 9-2014; 9-18
1871-1413
url http://hdl.handle.net/11336/11595
identifier_str_mv Fernandez, María Elena; Liron, Juan Pedro; Prando, Alberto; Rogberg Muñoz, Andres; Peral Garcia, Pilar; et al.; Evidence of association of a BTA20 region peaked in ISL1 with puberty in Angus bulls; Elsevier Science; Livestock Science; 167; 9-2014; 9-18
1871-1413
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.livsci.2014.05.009
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1871141314002455
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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