Reverse ultrasound-assisted emulsification-microextraction of macrolides from chicken fat followed by electrophoretic determination

Autores
Lorenzetti, Anabela Silvana; Lista, Adriana Guillermina; Domini, Claudia Elizabeth
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A new microextraction methodology, called reverse ultrasound-assisted emulsification-microextraction (R-USAEME) was developed to extract Tilmicosin (TILM) and Tylosin (TYL) from chicken fat samples, prior to their determination by capillary electrophoresis with UV-detection. The R-USAEME was based on the use of an aqueous ionic liquid ([Bmim]Cl) solution with sodium tartrate and sodium phosphate as extractant, applying an ultrasound probe (91 W; 7.5 min). A good linearity was obtained in a range from 35 to 200 μg kg−1 with relative standard deviations (RSDs) lower than 13% using matrix-matched calibration on five chicken fat samples. The quantification limits (LOQs), ranged from 17.4 to 55.0 μg kg−1 and from 22.1 to 47.0 μg kg−1 for TILM and TYL respectively. The obtained recoveries were between 73 and 117%. The analytical parameters clearly showed the applicability of the method for the extraction and quantification of macrolides in this complex biological sample.
Fil: Lorenzetti, Anabela Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Lista, Adriana Guillermina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Domini, Claudia Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Materia
CAPILLARY ELECTROPHORESIS
CHICKEN FAT
IONIC LIQUIDS
MACROLIDES
REVERSE ULTRASOUND-ASSISTED EMULSIFICATION-MICROEXTRACTION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/120457
Acceder
id CONICETDig_95464433678276febbf5766fd8a6718d
oai_identifier_str oai:ri.conicet.gov.ar:11336/120457
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Reverse ultrasound-assisted emulsification-microextraction of macrolides from chicken fat followed by electrophoretic determinationLorenzetti, Anabela SilvanaLista, Adriana GuillerminaDomini, Claudia ElizabethCAPILLARY ELECTROPHORESISCHICKEN FATIONIC LIQUIDSMACROLIDESREVERSE ULTRASOUND-ASSISTED EMULSIFICATION-MICROEXTRACTIONhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A new microextraction methodology, called reverse ultrasound-assisted emulsification-microextraction (R-USAEME) was developed to extract Tilmicosin (TILM) and Tylosin (TYL) from chicken fat samples, prior to their determination by capillary electrophoresis with UV-detection. The R-USAEME was based on the use of an aqueous ionic liquid ([Bmim]Cl) solution with sodium tartrate and sodium phosphate as extractant, applying an ultrasound probe (91 W; 7.5 min). A good linearity was obtained in a range from 35 to 200 μg kg−1 with relative standard deviations (RSDs) lower than 13% using matrix-matched calibration on five chicken fat samples. The quantification limits (LOQs), ranged from 17.4 to 55.0 μg kg−1 and from 22.1 to 47.0 μg kg−1 for TILM and TYL respectively. The obtained recoveries were between 73 and 117%. The analytical parameters clearly showed the applicability of the method for the extraction and quantification of macrolides in this complex biological sample.Fil: Lorenzetti, Anabela Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Lista, Adriana Guillermina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Domini, Claudia Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaElsevier Science2019-07-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/120457Lorenzetti, Anabela Silvana; Lista, Adriana Guillermina; Domini, Claudia Elizabeth; Reverse ultrasound-assisted emulsification-microextraction of macrolides from chicken fat followed by electrophoretic determination; Elsevier Science; LWT - Food Science and Technology; 113; 2-7-2019; 1-9; 1083340023-6438CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0023643819306760info:eu-repo/semantics/altIdentifier/doi/10.1016/j.lwt.2019.108334info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:45:21Zoai:ri.conicet.gov.ar:11336/120457instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:45:21.416CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Reverse ultrasound-assisted emulsification-microextraction of macrolides from chicken fat followed by electrophoretic determination
title Reverse ultrasound-assisted emulsification-microextraction of macrolides from chicken fat followed by electrophoretic determination
spellingShingle Reverse ultrasound-assisted emulsification-microextraction of macrolides from chicken fat followed by electrophoretic determination
Lorenzetti, Anabela Silvana
CAPILLARY ELECTROPHORESIS
CHICKEN FAT
IONIC LIQUIDS
MACROLIDES
REVERSE ULTRASOUND-ASSISTED EMULSIFICATION-MICROEXTRACTION
title_short Reverse ultrasound-assisted emulsification-microextraction of macrolides from chicken fat followed by electrophoretic determination
title_full Reverse ultrasound-assisted emulsification-microextraction of macrolides from chicken fat followed by electrophoretic determination
title_fullStr Reverse ultrasound-assisted emulsification-microextraction of macrolides from chicken fat followed by electrophoretic determination
title_full_unstemmed Reverse ultrasound-assisted emulsification-microextraction of macrolides from chicken fat followed by electrophoretic determination
title_sort Reverse ultrasound-assisted emulsification-microextraction of macrolides from chicken fat followed by electrophoretic determination
dc.creator.none.fl_str_mv Lorenzetti, Anabela Silvana
Lista, Adriana Guillermina
Domini, Claudia Elizabeth
author Lorenzetti, Anabela Silvana
author_facet Lorenzetti, Anabela Silvana
Lista, Adriana Guillermina
Domini, Claudia Elizabeth
author_role author
author2 Lista, Adriana Guillermina
Domini, Claudia Elizabeth
author2_role author
author
dc.subject.none.fl_str_mv CAPILLARY ELECTROPHORESIS
CHICKEN FAT
IONIC LIQUIDS
MACROLIDES
REVERSE ULTRASOUND-ASSISTED EMULSIFICATION-MICROEXTRACTION
topic CAPILLARY ELECTROPHORESIS
CHICKEN FAT
IONIC LIQUIDS
MACROLIDES
REVERSE ULTRASOUND-ASSISTED EMULSIFICATION-MICROEXTRACTION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A new microextraction methodology, called reverse ultrasound-assisted emulsification-microextraction (R-USAEME) was developed to extract Tilmicosin (TILM) and Tylosin (TYL) from chicken fat samples, prior to their determination by capillary electrophoresis with UV-detection. The R-USAEME was based on the use of an aqueous ionic liquid ([Bmim]Cl) solution with sodium tartrate and sodium phosphate as extractant, applying an ultrasound probe (91 W; 7.5 min). A good linearity was obtained in a range from 35 to 200 μg kg−1 with relative standard deviations (RSDs) lower than 13% using matrix-matched calibration on five chicken fat samples. The quantification limits (LOQs), ranged from 17.4 to 55.0 μg kg−1 and from 22.1 to 47.0 μg kg−1 for TILM and TYL respectively. The obtained recoveries were between 73 and 117%. The analytical parameters clearly showed the applicability of the method for the extraction and quantification of macrolides in this complex biological sample.
Fil: Lorenzetti, Anabela Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Lista, Adriana Guillermina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Domini, Claudia Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
description A new microextraction methodology, called reverse ultrasound-assisted emulsification-microextraction (R-USAEME) was developed to extract Tilmicosin (TILM) and Tylosin (TYL) from chicken fat samples, prior to their determination by capillary electrophoresis with UV-detection. The R-USAEME was based on the use of an aqueous ionic liquid ([Bmim]Cl) solution with sodium tartrate and sodium phosphate as extractant, applying an ultrasound probe (91 W; 7.5 min). A good linearity was obtained in a range from 35 to 200 μg kg−1 with relative standard deviations (RSDs) lower than 13% using matrix-matched calibration on five chicken fat samples. The quantification limits (LOQs), ranged from 17.4 to 55.0 μg kg−1 and from 22.1 to 47.0 μg kg−1 for TILM and TYL respectively. The obtained recoveries were between 73 and 117%. The analytical parameters clearly showed the applicability of the method for the extraction and quantification of macrolides in this complex biological sample.
publishDate 2019
dc.date.none.fl_str_mv 2019-07-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/120457
Lorenzetti, Anabela Silvana; Lista, Adriana Guillermina; Domini, Claudia Elizabeth; Reverse ultrasound-assisted emulsification-microextraction of macrolides from chicken fat followed by electrophoretic determination; Elsevier Science; LWT - Food Science and Technology; 113; 2-7-2019; 1-9; 108334
0023-6438
CONICET Digital
CONICET
url http://hdl.handle.net/11336/120457
identifier_str_mv Lorenzetti, Anabela Silvana; Lista, Adriana Guillermina; Domini, Claudia Elizabeth; Reverse ultrasound-assisted emulsification-microextraction of macrolides from chicken fat followed by electrophoretic determination; Elsevier Science; LWT - Food Science and Technology; 113; 2-7-2019; 1-9; 108334
0023-6438
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0023643819306760
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.lwt.2019.108334
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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