High-throughput determination of phenolic compounds in virgin olive oil using dispersive liquid-liquid microextraction-CZE microextraction-capillary zone electrophoresis
- Autores
- Monasterio, Romina Paula; Fernández, María de Los Ángeles; Silva, Maria Fernanda
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- This article reports a simple methodology using dispersive liquid-liquid microextraction (DLLME) combined with CZE. It has been applied for the simultaneous determination of phenolic compounds such as caffeic, gallic, vanillic, syringic, cinnamic, p-coumaric acids and oleuropein, apigenin, luteolin, 3-hydroxytyrosol, and tyrosol, in virgin olive oil (VOO). The optimized extraction conditions for 20 g of VOO were: extractant solvent: 400 L boric acid 30mMat pH 9.5; dispersive solvent: 300 L carbon tetrachloride; vortex: 8min; centrifugation: 3min. The composition of the BGE was optimized resulting in the selection of a solution made of 30mMboric acid at pH 9.5. As a strategy for on-line preconcentration a stacking step was applied, injecting a plug of water before sample injection. The short extraction time, centrifugation and electrophoretic steps allow the selective determination of phenolic compounds in VOO with satisfactory LODs (0.004-0.251 mg/kg), recoveries (89.4-101.0%), and RSD (less than 7.44% in peak area and less than 0.69% in migration time), compatible with the concentration levels present in the samples.
Fil: Monasterio, Romina Paula. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Mendoza. Instituto de Biologia Agricola de Mendoza; Argentina; Universidad Nacional de la Pampa. Facultad de Ciencias Exactas y Naturales. Departamento de Quimica; Argentina;
Fil: Fernández, María de Los Ángeles. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Mendoza. Instituto de Biologia Agricola de Mendoza; Argentina;
Fil: Silva, Maria Fernanda. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Mendoza. Instituto de Biologia Agricola de Mendoza; Argentina; - Materia
-
Cze
Dispersive Liquid-Liquid Microextraction
Capillary Zone Electrophoresis
Stacking
Virgin Olive Oil - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/2094
Ver los metadatos del registro completo
id |
CONICETDig_c0a25bcec1d4a688c58775c0c5832433 |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/2094 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
High-throughput determination of phenolic compounds in virgin olive oil using dispersive liquid-liquid microextraction-CZE microextraction-capillary zone electrophoresisMonasterio, Romina PaulaFernández, María de Los ÁngelesSilva, Maria FernandaCzeDispersive Liquid-Liquid MicroextractionCapillary Zone ElectrophoresisStackingVirgin Olive Oilhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1This article reports a simple methodology using dispersive liquid-liquid microextraction (DLLME) combined with CZE. It has been applied for the simultaneous determination of phenolic compounds such as caffeic, gallic, vanillic, syringic, cinnamic, p-coumaric acids and oleuropein, apigenin, luteolin, 3-hydroxytyrosol, and tyrosol, in virgin olive oil (VOO). The optimized extraction conditions for 20 g of VOO were: extractant solvent: 400 L boric acid 30mMat pH 9.5; dispersive solvent: 300 L carbon tetrachloride; vortex: 8min; centrifugation: 3min. The composition of the BGE was optimized resulting in the selection of a solution made of 30mMboric acid at pH 9.5. As a strategy for on-line preconcentration a stacking step was applied, injecting a plug of water before sample injection. The short extraction time, centrifugation and electrophoretic steps allow the selective determination of phenolic compounds in VOO with satisfactory LODs (0.004-0.251 mg/kg), recoveries (89.4-101.0%), and RSD (less than 7.44% in peak area and less than 0.69% in migration time), compatible with the concentration levels present in the samples.Fil: Monasterio, Romina Paula. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Mendoza. Instituto de Biologia Agricola de Mendoza; Argentina; Universidad Nacional de la Pampa. Facultad de Ciencias Exactas y Naturales. Departamento de Quimica; Argentina;Fil: Fernández, María de Los Ángeles. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Mendoza. Instituto de Biologia Agricola de Mendoza; Argentina;Fil: Silva, Maria Fernanda. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Mendoza. Instituto de Biologia Agricola de Mendoza; Argentina;Wiley Vch Verlag2013-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/2094Monasterio, Romina Paula; Fernández, María de Los Ángeles; Silva, Maria Fernanda; High-throughput determination of phenolic compounds in virgin olive oil using dispersive liquid-liquid microextraction-CZE microextraction-capillary zone electrophoresis; Wiley Vch Verlag; Electrophoresis; 34; 6-2013; 1836-18430173-0835enginfo:eu-repo/semantics/altIdentifier/doi/10.1002/elps.201300117info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/elps.201300117/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:07:23Zoai:ri.conicet.gov.ar:11336/2094instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:07:23.442CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
High-throughput determination of phenolic compounds in virgin olive oil using dispersive liquid-liquid microextraction-CZE microextraction-capillary zone electrophoresis |
title |
High-throughput determination of phenolic compounds in virgin olive oil using dispersive liquid-liquid microextraction-CZE microextraction-capillary zone electrophoresis |
spellingShingle |
High-throughput determination of phenolic compounds in virgin olive oil using dispersive liquid-liquid microextraction-CZE microextraction-capillary zone electrophoresis Monasterio, Romina Paula Cze Dispersive Liquid-Liquid Microextraction Capillary Zone Electrophoresis Stacking Virgin Olive Oil |
title_short |
High-throughput determination of phenolic compounds in virgin olive oil using dispersive liquid-liquid microextraction-CZE microextraction-capillary zone electrophoresis |
title_full |
High-throughput determination of phenolic compounds in virgin olive oil using dispersive liquid-liquid microextraction-CZE microextraction-capillary zone electrophoresis |
title_fullStr |
High-throughput determination of phenolic compounds in virgin olive oil using dispersive liquid-liquid microextraction-CZE microextraction-capillary zone electrophoresis |
title_full_unstemmed |
High-throughput determination of phenolic compounds in virgin olive oil using dispersive liquid-liquid microextraction-CZE microextraction-capillary zone electrophoresis |
title_sort |
High-throughput determination of phenolic compounds in virgin olive oil using dispersive liquid-liquid microextraction-CZE microextraction-capillary zone electrophoresis |
dc.creator.none.fl_str_mv |
Monasterio, Romina Paula Fernández, María de Los Ángeles Silva, Maria Fernanda |
author |
Monasterio, Romina Paula |
author_facet |
Monasterio, Romina Paula Fernández, María de Los Ángeles Silva, Maria Fernanda |
author_role |
author |
author2 |
Fernández, María de Los Ángeles Silva, Maria Fernanda |
author2_role |
author author |
dc.subject.none.fl_str_mv |
Cze Dispersive Liquid-Liquid Microextraction Capillary Zone Electrophoresis Stacking Virgin Olive Oil |
topic |
Cze Dispersive Liquid-Liquid Microextraction Capillary Zone Electrophoresis Stacking Virgin Olive Oil |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
This article reports a simple methodology using dispersive liquid-liquid microextraction (DLLME) combined with CZE. It has been applied for the simultaneous determination of phenolic compounds such as caffeic, gallic, vanillic, syringic, cinnamic, p-coumaric acids and oleuropein, apigenin, luteolin, 3-hydroxytyrosol, and tyrosol, in virgin olive oil (VOO). The optimized extraction conditions for 20 g of VOO were: extractant solvent: 400 L boric acid 30mMat pH 9.5; dispersive solvent: 300 L carbon tetrachloride; vortex: 8min; centrifugation: 3min. The composition of the BGE was optimized resulting in the selection of a solution made of 30mMboric acid at pH 9.5. As a strategy for on-line preconcentration a stacking step was applied, injecting a plug of water before sample injection. The short extraction time, centrifugation and electrophoretic steps allow the selective determination of phenolic compounds in VOO with satisfactory LODs (0.004-0.251 mg/kg), recoveries (89.4-101.0%), and RSD (less than 7.44% in peak area and less than 0.69% in migration time), compatible with the concentration levels present in the samples. Fil: Monasterio, Romina Paula. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Mendoza. Instituto de Biologia Agricola de Mendoza; Argentina; Universidad Nacional de la Pampa. Facultad de Ciencias Exactas y Naturales. Departamento de Quimica; Argentina; Fil: Fernández, María de Los Ángeles. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Mendoza. Instituto de Biologia Agricola de Mendoza; Argentina; Fil: Silva, Maria Fernanda. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Mendoza. Instituto de Biologia Agricola de Mendoza; Argentina; |
description |
This article reports a simple methodology using dispersive liquid-liquid microextraction (DLLME) combined with CZE. It has been applied for the simultaneous determination of phenolic compounds such as caffeic, gallic, vanillic, syringic, cinnamic, p-coumaric acids and oleuropein, apigenin, luteolin, 3-hydroxytyrosol, and tyrosol, in virgin olive oil (VOO). The optimized extraction conditions for 20 g of VOO were: extractant solvent: 400 L boric acid 30mMat pH 9.5; dispersive solvent: 300 L carbon tetrachloride; vortex: 8min; centrifugation: 3min. The composition of the BGE was optimized resulting in the selection of a solution made of 30mMboric acid at pH 9.5. As a strategy for on-line preconcentration a stacking step was applied, injecting a plug of water before sample injection. The short extraction time, centrifugation and electrophoretic steps allow the selective determination of phenolic compounds in VOO with satisfactory LODs (0.004-0.251 mg/kg), recoveries (89.4-101.0%), and RSD (less than 7.44% in peak area and less than 0.69% in migration time), compatible with the concentration levels present in the samples. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-06 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/2094 Monasterio, Romina Paula; Fernández, María de Los Ángeles; Silva, Maria Fernanda; High-throughput determination of phenolic compounds in virgin olive oil using dispersive liquid-liquid microextraction-CZE microextraction-capillary zone electrophoresis; Wiley Vch Verlag; Electrophoresis; 34; 6-2013; 1836-1843 0173-0835 |
url |
http://hdl.handle.net/11336/2094 |
identifier_str_mv |
Monasterio, Romina Paula; Fernández, María de Los Ángeles; Silva, Maria Fernanda; High-throughput determination of phenolic compounds in virgin olive oil using dispersive liquid-liquid microextraction-CZE microextraction-capillary zone electrophoresis; Wiley Vch Verlag; Electrophoresis; 34; 6-2013; 1836-1843 0173-0835 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1002/elps.201300117 info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/elps.201300117/abstract |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Wiley Vch Verlag |
publisher.none.fl_str_mv |
Wiley Vch Verlag |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1842270001589911552 |
score |
13.13397 |