High-throughput determination of phenolic compounds in virgin olive oil using dispersive liquid-liquid microextraction-CZE microextraction-capillary zone electrophoresis

Autores
Monasterio, Romina Paula; Fernández, María de Los Ángeles; Silva, Maria Fernanda
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This article reports a simple methodology using dispersive liquid-liquid microextraction (DLLME) combined with CZE. It has been applied for the simultaneous determination of phenolic compounds such as caffeic, gallic, vanillic, syringic, cinnamic, p-coumaric acids and oleuropein, apigenin, luteolin, 3-hydroxytyrosol, and tyrosol, in virgin olive oil (VOO). The optimized extraction conditions for 20 g of VOO were: extractant solvent: 400 L boric acid 30mMat pH 9.5; dispersive solvent: 300 L carbon tetrachloride; vortex: 8min; centrifugation: 3min. The composition of the BGE was optimized resulting in the selection of a solution made of 30mMboric acid at pH 9.5. As a strategy for on-line preconcentration a stacking step was applied, injecting a plug of water before sample injection. The short extraction time, centrifugation and electrophoretic steps allow the selective determination of phenolic compounds in VOO with satisfactory LODs (0.004-0.251 mg/kg), recoveries (89.4-101.0%), and RSD (less than 7.44% in peak area and less than 0.69% in migration time), compatible with the concentration levels present in the samples.
Fil: Monasterio, Romina Paula. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Mendoza. Instituto de Biologia Agricola de Mendoza; Argentina; Universidad Nacional de la Pampa. Facultad de Ciencias Exactas y Naturales. Departamento de Quimica; Argentina;
Fil: Fernández, María de Los Ángeles. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Mendoza. Instituto de Biologia Agricola de Mendoza; Argentina;
Fil: Silva, Maria Fernanda. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Mendoza. Instituto de Biologia Agricola de Mendoza; Argentina;
Materia
Cze
Dispersive Liquid-Liquid Microextraction
Capillary Zone Electrophoresis
Stacking
Virgin Olive Oil
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/2094
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/2094
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling High-throughput determination of phenolic compounds in virgin olive oil using dispersive liquid-liquid microextraction-CZE microextraction-capillary zone electrophoresisMonasterio, Romina PaulaFernández, María de Los ÁngelesSilva, Maria FernandaCzeDispersive Liquid-Liquid MicroextractionCapillary Zone ElectrophoresisStackingVirgin Olive Oilhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1This article reports a simple methodology using dispersive liquid-liquid microextraction (DLLME) combined with CZE. It has been applied for the simultaneous determination of phenolic compounds such as caffeic, gallic, vanillic, syringic, cinnamic, p-coumaric acids and oleuropein, apigenin, luteolin, 3-hydroxytyrosol, and tyrosol, in virgin olive oil (VOO). The optimized extraction conditions for 20 g of VOO were: extractant solvent: 400 L boric acid 30mMat pH 9.5; dispersive solvent: 300 L carbon tetrachloride; vortex: 8min; centrifugation: 3min. The composition of the BGE was optimized resulting in the selection of a solution made of 30mMboric acid at pH 9.5. As a strategy for on-line preconcentration a stacking step was applied, injecting a plug of water before sample injection. The short extraction time, centrifugation and electrophoretic steps allow the selective determination of phenolic compounds in VOO with satisfactory LODs (0.004-0.251 mg/kg), recoveries (89.4-101.0%), and RSD (less than 7.44% in peak area and less than 0.69% in migration time), compatible with the concentration levels present in the samples.Fil: Monasterio, Romina Paula. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Mendoza. Instituto de Biologia Agricola de Mendoza; Argentina; Universidad Nacional de la Pampa. Facultad de Ciencias Exactas y Naturales. Departamento de Quimica; Argentina;Fil: Fernández, María de Los Ángeles. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Mendoza. Instituto de Biologia Agricola de Mendoza; Argentina;Fil: Silva, Maria Fernanda. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Mendoza. Instituto de Biologia Agricola de Mendoza; Argentina;Wiley Vch Verlag2013-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/2094Monasterio, Romina Paula; Fernández, María de Los Ángeles; Silva, Maria Fernanda; High-throughput determination of phenolic compounds in virgin olive oil using dispersive liquid-liquid microextraction-CZE microextraction-capillary zone electrophoresis; Wiley Vch Verlag; Electrophoresis; 34; 6-2013; 1836-18430173-0835enginfo:eu-repo/semantics/altIdentifier/doi/10.1002/elps.201300117info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/elps.201300117/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:33:19Zoai:ri.conicet.gov.ar:11336/2094instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:33:19.773CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv High-throughput determination of phenolic compounds in virgin olive oil using dispersive liquid-liquid microextraction-CZE microextraction-capillary zone electrophoresis
title High-throughput determination of phenolic compounds in virgin olive oil using dispersive liquid-liquid microextraction-CZE microextraction-capillary zone electrophoresis
spellingShingle High-throughput determination of phenolic compounds in virgin olive oil using dispersive liquid-liquid microextraction-CZE microextraction-capillary zone electrophoresis
Monasterio, Romina Paula
Cze
Dispersive Liquid-Liquid Microextraction
Capillary Zone Electrophoresis
Stacking
Virgin Olive Oil
title_short High-throughput determination of phenolic compounds in virgin olive oil using dispersive liquid-liquid microextraction-CZE microextraction-capillary zone electrophoresis
title_full High-throughput determination of phenolic compounds in virgin olive oil using dispersive liquid-liquid microextraction-CZE microextraction-capillary zone electrophoresis
title_fullStr High-throughput determination of phenolic compounds in virgin olive oil using dispersive liquid-liquid microextraction-CZE microextraction-capillary zone electrophoresis
title_full_unstemmed High-throughput determination of phenolic compounds in virgin olive oil using dispersive liquid-liquid microextraction-CZE microextraction-capillary zone electrophoresis
title_sort High-throughput determination of phenolic compounds in virgin olive oil using dispersive liquid-liquid microextraction-CZE microextraction-capillary zone electrophoresis
dc.creator.none.fl_str_mv Monasterio, Romina Paula
Fernández, María de Los Ángeles
Silva, Maria Fernanda
author Monasterio, Romina Paula
author_facet Monasterio, Romina Paula
Fernández, María de Los Ángeles
Silva, Maria Fernanda
author_role author
author2 Fernández, María de Los Ángeles
Silva, Maria Fernanda
author2_role author
author
dc.subject.none.fl_str_mv Cze
Dispersive Liquid-Liquid Microextraction
Capillary Zone Electrophoresis
Stacking
Virgin Olive Oil
topic Cze
Dispersive Liquid-Liquid Microextraction
Capillary Zone Electrophoresis
Stacking
Virgin Olive Oil
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv This article reports a simple methodology using dispersive liquid-liquid microextraction (DLLME) combined with CZE. It has been applied for the simultaneous determination of phenolic compounds such as caffeic, gallic, vanillic, syringic, cinnamic, p-coumaric acids and oleuropein, apigenin, luteolin, 3-hydroxytyrosol, and tyrosol, in virgin olive oil (VOO). The optimized extraction conditions for 20 g of VOO were: extractant solvent: 400 L boric acid 30mMat pH 9.5; dispersive solvent: 300 L carbon tetrachloride; vortex: 8min; centrifugation: 3min. The composition of the BGE was optimized resulting in the selection of a solution made of 30mMboric acid at pH 9.5. As a strategy for on-line preconcentration a stacking step was applied, injecting a plug of water before sample injection. The short extraction time, centrifugation and electrophoretic steps allow the selective determination of phenolic compounds in VOO with satisfactory LODs (0.004-0.251 mg/kg), recoveries (89.4-101.0%), and RSD (less than 7.44% in peak area and less than 0.69% in migration time), compatible with the concentration levels present in the samples.
Fil: Monasterio, Romina Paula. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Mendoza. Instituto de Biologia Agricola de Mendoza; Argentina; Universidad Nacional de la Pampa. Facultad de Ciencias Exactas y Naturales. Departamento de Quimica; Argentina;
Fil: Fernández, María de Los Ángeles. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Mendoza. Instituto de Biologia Agricola de Mendoza; Argentina;
Fil: Silva, Maria Fernanda. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Mendoza. Instituto de Biologia Agricola de Mendoza; Argentina;
description This article reports a simple methodology using dispersive liquid-liquid microextraction (DLLME) combined with CZE. It has been applied for the simultaneous determination of phenolic compounds such as caffeic, gallic, vanillic, syringic, cinnamic, p-coumaric acids and oleuropein, apigenin, luteolin, 3-hydroxytyrosol, and tyrosol, in virgin olive oil (VOO). The optimized extraction conditions for 20 g of VOO were: extractant solvent: 400 L boric acid 30mMat pH 9.5; dispersive solvent: 300 L carbon tetrachloride; vortex: 8min; centrifugation: 3min. The composition of the BGE was optimized resulting in the selection of a solution made of 30mMboric acid at pH 9.5. As a strategy for on-line preconcentration a stacking step was applied, injecting a plug of water before sample injection. The short extraction time, centrifugation and electrophoretic steps allow the selective determination of phenolic compounds in VOO with satisfactory LODs (0.004-0.251 mg/kg), recoveries (89.4-101.0%), and RSD (less than 7.44% in peak area and less than 0.69% in migration time), compatible with the concentration levels present in the samples.
publishDate 2013
dc.date.none.fl_str_mv 2013-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/2094
Monasterio, Romina Paula; Fernández, María de Los Ángeles; Silva, Maria Fernanda; High-throughput determination of phenolic compounds in virgin olive oil using dispersive liquid-liquid microextraction-CZE microextraction-capillary zone electrophoresis; Wiley Vch Verlag; Electrophoresis; 34; 6-2013; 1836-1843
0173-0835
url http://hdl.handle.net/11336/2094
identifier_str_mv Monasterio, Romina Paula; Fernández, María de Los Ángeles; Silva, Maria Fernanda; High-throughput determination of phenolic compounds in virgin olive oil using dispersive liquid-liquid microextraction-CZE microextraction-capillary zone electrophoresis; Wiley Vch Verlag; Electrophoresis; 34; 6-2013; 1836-1843
0173-0835
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/elps.201300117
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/elps.201300117/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Vch Verlag
publisher.none.fl_str_mv Wiley Vch Verlag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614348515835904
score 13.070432

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