Compact and reflective light-sheet microscopy for long-term imaging of living embryos
- Autores
- Moretti, Bruno; Müller, Nicolás Pablo; Wappner, Marcos; Grecco, Hernan Edgardo
- Año de publicación
- 2020
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The development of light-sheet fluorescence microscopy has been a revolution for developmental biology as it allows long-term imaging during embryonic development. An important reason behind the quick adoption has been the availability of open hardware alternatives. In this work, we present a robust and compact version of a light-sheet fluorescence microscope that is easy to assemble and requires little to no maintenance. An important aspect of the design is that the illumination unit consists of reflective elements, thereby reducing chromatic aberrations an order of magnitude as compared to refractive counterparts.
Fil: Moretti, Bruno. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
Fil: Müller, Nicolás Pablo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina
Fil: Wappner, Marcos. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Grecco, Hernan Edgardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina - Materia
-
Light-sheet fluorescence microscopy
compact
reflective
single plane illumination microscopy - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/146748
Ver los metadatos del registro completo
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Compact and reflective light-sheet microscopy for long-term imaging of living embryosMoretti, BrunoMüller, Nicolás PabloWappner, MarcosGrecco, Hernan EdgardoLight-sheet fluorescence microscopycompactreflectivesingle plane illumination microscopyhttps://purl.org/becyt/ford/1.3https://purl.org/becyt/ford/1The development of light-sheet fluorescence microscopy has been a revolution for developmental biology as it allows long-term imaging during embryonic development. An important reason behind the quick adoption has been the availability of open hardware alternatives. In this work, we present a robust and compact version of a light-sheet fluorescence microscope that is easy to assemble and requires little to no maintenance. An important aspect of the design is that the illumination unit consists of reflective elements, thereby reducing chromatic aberrations an order of magnitude as compared to refractive counterparts.Fil: Moretti, Bruno. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; ArgentinaFil: Müller, Nicolás Pablo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; ArgentinaFil: Wappner, Marcos. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Grecco, Hernan Edgardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; ArgentinaOptical Society of America2020-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/146748Moretti, Bruno; Müller, Nicolás Pablo; Wappner, Marcos; Grecco, Hernan Edgardo; Compact and reflective light-sheet microscopy for long-term imaging of living embryos; Optical Society of America; Applied Optics; 59; 13; 5-2020; D89-D942155-3165CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.osapublishing.org/abstract.cfm?URI=ao-59-13-D89info:eu-repo/semantics/altIdentifier/doi/10.1364/AO.383026info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:24:33Zoai:ri.conicet.gov.ar:11336/146748instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:24:33.464CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Compact and reflective light-sheet microscopy for long-term imaging of living embryos |
title |
Compact and reflective light-sheet microscopy for long-term imaging of living embryos |
spellingShingle |
Compact and reflective light-sheet microscopy for long-term imaging of living embryos Moretti, Bruno Light-sheet fluorescence microscopy compact reflective single plane illumination microscopy |
title_short |
Compact and reflective light-sheet microscopy for long-term imaging of living embryos |
title_full |
Compact and reflective light-sheet microscopy for long-term imaging of living embryos |
title_fullStr |
Compact and reflective light-sheet microscopy for long-term imaging of living embryos |
title_full_unstemmed |
Compact and reflective light-sheet microscopy for long-term imaging of living embryos |
title_sort |
Compact and reflective light-sheet microscopy for long-term imaging of living embryos |
dc.creator.none.fl_str_mv |
Moretti, Bruno Müller, Nicolás Pablo Wappner, Marcos Grecco, Hernan Edgardo |
author |
Moretti, Bruno |
author_facet |
Moretti, Bruno Müller, Nicolás Pablo Wappner, Marcos Grecco, Hernan Edgardo |
author_role |
author |
author2 |
Müller, Nicolás Pablo Wappner, Marcos Grecco, Hernan Edgardo |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Light-sheet fluorescence microscopy compact reflective single plane illumination microscopy |
topic |
Light-sheet fluorescence microscopy compact reflective single plane illumination microscopy |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.3 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
The development of light-sheet fluorescence microscopy has been a revolution for developmental biology as it allows long-term imaging during embryonic development. An important reason behind the quick adoption has been the availability of open hardware alternatives. In this work, we present a robust and compact version of a light-sheet fluorescence microscope that is easy to assemble and requires little to no maintenance. An important aspect of the design is that the illumination unit consists of reflective elements, thereby reducing chromatic aberrations an order of magnitude as compared to refractive counterparts. Fil: Moretti, Bruno. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina Fil: Müller, Nicolás Pablo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina Fil: Wappner, Marcos. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Grecco, Hernan Edgardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina |
description |
The development of light-sheet fluorescence microscopy has been a revolution for developmental biology as it allows long-term imaging during embryonic development. An important reason behind the quick adoption has been the availability of open hardware alternatives. In this work, we present a robust and compact version of a light-sheet fluorescence microscope that is easy to assemble and requires little to no maintenance. An important aspect of the design is that the illumination unit consists of reflective elements, thereby reducing chromatic aberrations an order of magnitude as compared to refractive counterparts. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-05 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/146748 Moretti, Bruno; Müller, Nicolás Pablo; Wappner, Marcos; Grecco, Hernan Edgardo; Compact and reflective light-sheet microscopy for long-term imaging of living embryos; Optical Society of America; Applied Optics; 59; 13; 5-2020; D89-D94 2155-3165 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/146748 |
identifier_str_mv |
Moretti, Bruno; Müller, Nicolás Pablo; Wappner, Marcos; Grecco, Hernan Edgardo; Compact and reflective light-sheet microscopy for long-term imaging of living embryos; Optical Society of America; Applied Optics; 59; 13; 5-2020; D89-D94 2155-3165 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.osapublishing.org/abstract.cfm?URI=ao-59-13-D89 info:eu-repo/semantics/altIdentifier/doi/10.1364/AO.383026 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Optical Society of America |
publisher.none.fl_str_mv |
Optical Society of America |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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score |
13.22299 |