Extending resolution within a single imaging frame
- Autores
- Torres García, Esley; Pinto Cámara, Raúl; Linares, Alejandro; Martínez, Damián; Abonza, Víctor; Brito Alarcón, Eduardo; Calcines Cruz, Carlos; Valdés Galindo, Gustavo; Torres, David; Jabloñski, Martina; Torres Martínez, Héctor H.; Martínez, José L.; Hernández, Haydee O.; Ocelotl Oviedo, José P.; Garcés, Yasel; Barchi, Marco; D'Antuono, Rocco; Bošković, Ana; Dubrovsky, Joseph G.; Darszon, Alberto; Buffone, Mariano Gabriel; Rodríguez Morales, Roberto; Rendon Mancha, Juan Manuel; Wood, Christopher D.; Hernández García, Armando; Krapf, Diego; Crevenna, Álvaro H.; Guerrero, Adán
- Año de publicación
- 2022
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The resolution of fluorescence microscopy images is limited by the physical properties of light. In the last decade, numerous super-resolution microscopy (SRM) approaches have been proposed to deal with such hindrance. Here we present Mean-Shift Super Resolution (MSSR), a new SRM algorithm based on the Mean Shift theory, which extends spatial resolution of single fluorescence images beyond the diffraction limit of light. MSSR works on low and high fluorophore densities, is not limited by the architecture of the optical setup and is applicable to single images as well as temporal series. The theoretical limit of spatial resolution, based on optimized real-world imaging conditions and analysis of temporal image stacks, has been measured to be 40 nm. Furthermore, MSSR has denoising capabilities that outperform other SRM approaches. Along with its wide accessibility, MSSR is a powerful, flexible, and generic tool for multidimensional and live cell imaging applications.
Fil: Torres García, Esley. Universidad Nacional Autónoma de México; México
Fil: Pinto Cámara, Raúl. Universidad Nacional Autónoma de México; México
Fil: Linares, Alejandro. Universidad Nacional Autónoma de México; México
Fil: Martínez, Damián. Universidad Nacional Autónoma de México; México
Fil: Abonza, Víctor. Universidad Nacional Autónoma de México; México
Fil: Brito Alarcón, Eduardo. Universidad Nacional Autónoma de México; México
Fil: Calcines Cruz, Carlos. Universidad Nacional Autónoma de México; México
Fil: Valdés Galindo, Gustavo. Universidad Nacional Autónoma de México; México
Fil: Torres, David. Universidad Nacional Autónoma de México; México
Fil: Jabloñski, Martina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Torres Martínez, Héctor H.. Universidad Nacional Autónoma de México; México
Fil: Martínez, José L.. Universidad Nacional Autónoma de México; México
Fil: Hernández, Haydee O.. Universidad Nacional Autónoma de México; México
Fil: Ocelotl Oviedo, José P.. Universidad Nacional Autónoma de México; México
Fil: Garcés, Yasel. Universidad Nacional Autónoma de México; México
Fil: Barchi, Marco. University of Rome Tor Vergata; Italia
Fil: D'Antuono, Rocco. Crick Advanced Light Microscopy Facility; Reino Unido
Fil: Bošković, Ana. European Molecular Biology Laboratory; Alemania
Fil: Dubrovsky, Joseph G.. Universidad Nacional Autónoma de México; México
Fil: Darszon, Alberto. Universidad Nacional Autónoma de México; México
Fil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Rodríguez Morales, Roberto. No especifíca;
Fil: Rendon Mancha, Juan Manuel. Universidad Autónoma del Estado de Morelos; México
Fil: Wood, Christopher D.. Universidad Autónoma del Estado de Morelos; México
Fil: Hernández García, Armando. Universidad Autónoma del Estado de Morelos; México
Fil: Krapf, Diego. University of Colorado; Estados Unidos
Fil: Crevenna, Álvaro H.. European Molecular Biology Laboratory; Italia
Fil: Guerrero, Adán. Universidad Autónoma del Estado de Morelos; México - Materia
-
SUPER RESOLUTION MICROSCOPY
DIFFRACTION LIMIT
SINGLE FRAME
MEAN SHIFT
FLUORESCENCEN MICROSCOPY
LIVE-CELL IMAGING
LUNG-CANCER - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/210111
Ver los metadatos del registro completo
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Extending resolution within a single imaging frameTorres García, EsleyPinto Cámara, RaúlLinares, AlejandroMartínez, DamiánAbonza, VíctorBrito Alarcón, EduardoCalcines Cruz, CarlosValdés Galindo, GustavoTorres, DavidJabloñski, MartinaTorres Martínez, Héctor H.Martínez, José L.Hernández, Haydee O.Ocelotl Oviedo, José P.Garcés, YaselBarchi, MarcoD'Antuono, RoccoBošković, AnaDubrovsky, Joseph G.Darszon, AlbertoBuffone, Mariano GabrielRodríguez Morales, RobertoRendon Mancha, Juan ManuelWood, Christopher D.Hernández García, ArmandoKrapf, DiegoCrevenna, Álvaro H.Guerrero, AdánSUPER RESOLUTION MICROSCOPYDIFFRACTION LIMITSINGLE FRAMEMEAN SHIFTFLUORESCENCEN MICROSCOPYLIVE-CELL IMAGINGLUNG-CANCERhttps://purl.org/becyt/ford/1.1https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The resolution of fluorescence microscopy images is limited by the physical properties of light. In the last decade, numerous super-resolution microscopy (SRM) approaches have been proposed to deal with such hindrance. Here we present Mean-Shift Super Resolution (MSSR), a new SRM algorithm based on the Mean Shift theory, which extends spatial resolution of single fluorescence images beyond the diffraction limit of light. MSSR works on low and high fluorophore densities, is not limited by the architecture of the optical setup and is applicable to single images as well as temporal series. The theoretical limit of spatial resolution, based on optimized real-world imaging conditions and analysis of temporal image stacks, has been measured to be 40 nm. Furthermore, MSSR has denoising capabilities that outperform other SRM approaches. Along with its wide accessibility, MSSR is a powerful, flexible, and generic tool for multidimensional and live cell imaging applications.Fil: Torres García, Esley. Universidad Nacional Autónoma de México; MéxicoFil: Pinto Cámara, Raúl. Universidad Nacional Autónoma de México; MéxicoFil: Linares, Alejandro. Universidad Nacional Autónoma de México; MéxicoFil: Martínez, Damián. Universidad Nacional Autónoma de México; MéxicoFil: Abonza, Víctor. Universidad Nacional Autónoma de México; MéxicoFil: Brito Alarcón, Eduardo. Universidad Nacional Autónoma de México; MéxicoFil: Calcines Cruz, Carlos. Universidad Nacional Autónoma de México; MéxicoFil: Valdés Galindo, Gustavo. Universidad Nacional Autónoma de México; MéxicoFil: Torres, David. Universidad Nacional Autónoma de México; MéxicoFil: Jabloñski, Martina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Torres Martínez, Héctor H.. Universidad Nacional Autónoma de México; MéxicoFil: Martínez, José L.. Universidad Nacional Autónoma de México; MéxicoFil: Hernández, Haydee O.. Universidad Nacional Autónoma de México; MéxicoFil: Ocelotl Oviedo, José P.. Universidad Nacional Autónoma de México; MéxicoFil: Garcés, Yasel. Universidad Nacional Autónoma de México; MéxicoFil: Barchi, Marco. University of Rome Tor Vergata; ItaliaFil: D'Antuono, Rocco. Crick Advanced Light Microscopy Facility; Reino UnidoFil: Bošković, Ana. European Molecular Biology Laboratory; AlemaniaFil: Dubrovsky, Joseph G.. Universidad Nacional Autónoma de México; MéxicoFil: Darszon, Alberto. Universidad Nacional Autónoma de México; MéxicoFil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Rodríguez Morales, Roberto. No especifíca;Fil: Rendon Mancha, Juan Manuel. Universidad Autónoma del Estado de Morelos; MéxicoFil: Wood, Christopher D.. Universidad Autónoma del Estado de Morelos; MéxicoFil: Hernández García, Armando. Universidad Autónoma del Estado de Morelos; MéxicoFil: Krapf, Diego. University of Colorado; Estados UnidosFil: Crevenna, Álvaro H.. European Molecular Biology Laboratory; ItaliaFil: Guerrero, Adán. Universidad Autónoma del Estado de Morelos; MéxicoNature Publishing Group2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/210111Torres García, Esley; Pinto Cámara, Raúl; Linares, Alejandro; Martínez, Damián; Abonza, Víctor; et al.; Extending resolution within a single imaging frame; Nature Publishing Group; Nature Communications; 13; 1; 2022; 1-222041-1723CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1038/s41467-022-34693-9info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:13:30Zoai:ri.conicet.gov.ar:11336/210111instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:13:30.461CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Extending resolution within a single imaging frame |
| title |
Extending resolution within a single imaging frame |
| spellingShingle |
Extending resolution within a single imaging frame Torres García, Esley SUPER RESOLUTION MICROSCOPY DIFFRACTION LIMIT SINGLE FRAME MEAN SHIFT FLUORESCENCEN MICROSCOPY LIVE-CELL IMAGING LUNG-CANCER |
| title_short |
Extending resolution within a single imaging frame |
| title_full |
Extending resolution within a single imaging frame |
| title_fullStr |
Extending resolution within a single imaging frame |
| title_full_unstemmed |
Extending resolution within a single imaging frame |
| title_sort |
Extending resolution within a single imaging frame |
| dc.creator.none.fl_str_mv |
Torres García, Esley Pinto Cámara, Raúl Linares, Alejandro Martínez, Damián Abonza, Víctor Brito Alarcón, Eduardo Calcines Cruz, Carlos Valdés Galindo, Gustavo Torres, David Jabloñski, Martina Torres Martínez, Héctor H. Martínez, José L. Hernández, Haydee O. Ocelotl Oviedo, José P. Garcés, Yasel Barchi, Marco D'Antuono, Rocco Bošković, Ana Dubrovsky, Joseph G. Darszon, Alberto Buffone, Mariano Gabriel Rodríguez Morales, Roberto Rendon Mancha, Juan Manuel Wood, Christopher D. Hernández García, Armando Krapf, Diego Crevenna, Álvaro H. Guerrero, Adán |
| author |
Torres García, Esley |
| author_facet |
Torres García, Esley Pinto Cámara, Raúl Linares, Alejandro Martínez, Damián Abonza, Víctor Brito Alarcón, Eduardo Calcines Cruz, Carlos Valdés Galindo, Gustavo Torres, David Jabloñski, Martina Torres Martínez, Héctor H. Martínez, José L. Hernández, Haydee O. Ocelotl Oviedo, José P. Garcés, Yasel Barchi, Marco D'Antuono, Rocco Bošković, Ana Dubrovsky, Joseph G. Darszon, Alberto Buffone, Mariano Gabriel Rodríguez Morales, Roberto Rendon Mancha, Juan Manuel Wood, Christopher D. Hernández García, Armando Krapf, Diego Crevenna, Álvaro H. Guerrero, Adán |
| author_role |
author |
| author2 |
Pinto Cámara, Raúl Linares, Alejandro Martínez, Damián Abonza, Víctor Brito Alarcón, Eduardo Calcines Cruz, Carlos Valdés Galindo, Gustavo Torres, David Jabloñski, Martina Torres Martínez, Héctor H. Martínez, José L. Hernández, Haydee O. Ocelotl Oviedo, José P. Garcés, Yasel Barchi, Marco D'Antuono, Rocco Bošković, Ana Dubrovsky, Joseph G. Darszon, Alberto Buffone, Mariano Gabriel Rodríguez Morales, Roberto Rendon Mancha, Juan Manuel Wood, Christopher D. Hernández García, Armando Krapf, Diego Crevenna, Álvaro H. Guerrero, Adán |
| author2_role |
author author author author author author author author author author author author author author author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
SUPER RESOLUTION MICROSCOPY DIFFRACTION LIMIT SINGLE FRAME MEAN SHIFT FLUORESCENCEN MICROSCOPY LIVE-CELL IMAGING LUNG-CANCER |
| topic |
SUPER RESOLUTION MICROSCOPY DIFFRACTION LIMIT SINGLE FRAME MEAN SHIFT FLUORESCENCEN MICROSCOPY LIVE-CELL IMAGING LUNG-CANCER |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.1 https://purl.org/becyt/ford/1 https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
| dc.description.none.fl_txt_mv |
The resolution of fluorescence microscopy images is limited by the physical properties of light. In the last decade, numerous super-resolution microscopy (SRM) approaches have been proposed to deal with such hindrance. Here we present Mean-Shift Super Resolution (MSSR), a new SRM algorithm based on the Mean Shift theory, which extends spatial resolution of single fluorescence images beyond the diffraction limit of light. MSSR works on low and high fluorophore densities, is not limited by the architecture of the optical setup and is applicable to single images as well as temporal series. The theoretical limit of spatial resolution, based on optimized real-world imaging conditions and analysis of temporal image stacks, has been measured to be 40 nm. Furthermore, MSSR has denoising capabilities that outperform other SRM approaches. Along with its wide accessibility, MSSR is a powerful, flexible, and generic tool for multidimensional and live cell imaging applications. Fil: Torres García, Esley. Universidad Nacional Autónoma de México; México Fil: Pinto Cámara, Raúl. Universidad Nacional Autónoma de México; México Fil: Linares, Alejandro. Universidad Nacional Autónoma de México; México Fil: Martínez, Damián. Universidad Nacional Autónoma de México; México Fil: Abonza, Víctor. Universidad Nacional Autónoma de México; México Fil: Brito Alarcón, Eduardo. Universidad Nacional Autónoma de México; México Fil: Calcines Cruz, Carlos. Universidad Nacional Autónoma de México; México Fil: Valdés Galindo, Gustavo. Universidad Nacional Autónoma de México; México Fil: Torres, David. Universidad Nacional Autónoma de México; México Fil: Jabloñski, Martina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina Fil: Torres Martínez, Héctor H.. Universidad Nacional Autónoma de México; México Fil: Martínez, José L.. Universidad Nacional Autónoma de México; México Fil: Hernández, Haydee O.. Universidad Nacional Autónoma de México; México Fil: Ocelotl Oviedo, José P.. Universidad Nacional Autónoma de México; México Fil: Garcés, Yasel. Universidad Nacional Autónoma de México; México Fil: Barchi, Marco. University of Rome Tor Vergata; Italia Fil: D'Antuono, Rocco. Crick Advanced Light Microscopy Facility; Reino Unido Fil: Bošković, Ana. European Molecular Biology Laboratory; Alemania Fil: Dubrovsky, Joseph G.. Universidad Nacional Autónoma de México; México Fil: Darszon, Alberto. Universidad Nacional Autónoma de México; México Fil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina Fil: Rodríguez Morales, Roberto. No especifíca; Fil: Rendon Mancha, Juan Manuel. Universidad Autónoma del Estado de Morelos; México Fil: Wood, Christopher D.. Universidad Autónoma del Estado de Morelos; México Fil: Hernández García, Armando. Universidad Autónoma del Estado de Morelos; México Fil: Krapf, Diego. University of Colorado; Estados Unidos Fil: Crevenna, Álvaro H.. European Molecular Biology Laboratory; Italia Fil: Guerrero, Adán. Universidad Autónoma del Estado de Morelos; México |
| description |
The resolution of fluorescence microscopy images is limited by the physical properties of light. In the last decade, numerous super-resolution microscopy (SRM) approaches have been proposed to deal with such hindrance. Here we present Mean-Shift Super Resolution (MSSR), a new SRM algorithm based on the Mean Shift theory, which extends spatial resolution of single fluorescence images beyond the diffraction limit of light. MSSR works on low and high fluorophore densities, is not limited by the architecture of the optical setup and is applicable to single images as well as temporal series. The theoretical limit of spatial resolution, based on optimized real-world imaging conditions and analysis of temporal image stacks, has been measured to be 40 nm. Furthermore, MSSR has denoising capabilities that outperform other SRM approaches. Along with its wide accessibility, MSSR is a powerful, flexible, and generic tool for multidimensional and live cell imaging applications. |
| publishDate |
2022 |
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2022 |
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article |
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http://hdl.handle.net/11336/210111 Torres García, Esley; Pinto Cámara, Raúl; Linares, Alejandro; Martínez, Damián; Abonza, Víctor; et al.; Extending resolution within a single imaging frame; Nature Publishing Group; Nature Communications; 13; 1; 2022; 1-22 2041-1723 CONICET Digital CONICET |
| url |
http://hdl.handle.net/11336/210111 |
| identifier_str_mv |
Torres García, Esley; Pinto Cámara, Raúl; Linares, Alejandro; Martínez, Damián; Abonza, Víctor; et al.; Extending resolution within a single imaging frame; Nature Publishing Group; Nature Communications; 13; 1; 2022; 1-22 2041-1723 CONICET Digital CONICET |
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Nature Publishing Group |
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Nature Publishing Group |
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