Improving image contrast in fluorescence microscopy with nanostructured substrates
- Autores
- Brunstein, Maia; Cattoni, Andrea; Estrada, Laura Cecilia; Yacomotti, Alejandro M.
- Año de publicación
- 2015
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Metallic and dielectric nanostructures can show sharp contrastedresonances, sensitive to the environment, and high field enhancement insub-wavelength volumes. For this reason, these structures are commonlyused as molecular sensors. Only few works have focused on theirapplication in optical microscopy, in particular in superresolution. In thiswork we have designed, fabricated and optically tested a nanostructuredTiO2 substrate, fabricated by direct embossing of TiO2 derived film, as asubstrate for fluorescence microscopy. Moreover, using numericalsimulations, we have compared the signal to background noise with respectto other metallo-dielectric structures. We show that the TiO2 structure is agood candidate for reducing the thickness of the fluorescence excitationdown to ~100 nm. Therefore, this substrate can be used to obtain TotalInternal Reflection (TIRF) axial resolution without a TIRF-Microscopysystem.
Fil: Brunstein, Maia. Centre National de la Recherche Scientifique; Francia. Université Paris-Saclay; Francia
Fil: Cattoni, Andrea. Centre National de la Recherche Scientifique; Francia. Université Paris-Saclay; Francia
Fil: Estrada, Laura Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
Fil: Yacomotti, Alejandro M.. Centre National de la Recherche Scientifique; Francia. Université Paris-Saclay; Francia - Materia
-
FLUORESCENCE MICROSCOPY
ILLUMINATION DESIGN
RESONATORS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/46232
Ver los metadatos del registro completo
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Improving image contrast in fluorescence microscopy with nanostructured substratesBrunstein, MaiaCattoni, AndreaEstrada, Laura CeciliaYacomotti, Alejandro M.FLUORESCENCE MICROSCOPYILLUMINATION DESIGNRESONATORShttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2Metallic and dielectric nanostructures can show sharp contrastedresonances, sensitive to the environment, and high field enhancement insub-wavelength volumes. For this reason, these structures are commonlyused as molecular sensors. Only few works have focused on theirapplication in optical microscopy, in particular in superresolution. In thiswork we have designed, fabricated and optically tested a nanostructuredTiO2 substrate, fabricated by direct embossing of TiO2 derived film, as asubstrate for fluorescence microscopy. Moreover, using numericalsimulations, we have compared the signal to background noise with respectto other metallo-dielectric structures. We show that the TiO2 structure is agood candidate for reducing the thickness of the fluorescence excitationdown to ~100 nm. Therefore, this substrate can be used to obtain TotalInternal Reflection (TIRF) axial resolution without a TIRF-Microscopysystem.Fil: Brunstein, Maia. Centre National de la Recherche Scientifique; Francia. Université Paris-Saclay; FranciaFil: Cattoni, Andrea. Centre National de la Recherche Scientifique; Francia. Université Paris-Saclay; FranciaFil: Estrada, Laura Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; ArgentinaFil: Yacomotti, Alejandro M.. Centre National de la Recherche Scientifique; Francia. Université Paris-Saclay; FranciaOptical Society of America2015-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/46232Brunstein, Maia; Cattoni, Andrea; Estrada, Laura Cecilia; Yacomotti, Alejandro M.; Improving image contrast in fluorescence microscopy with nanostructured substrates; Optical Society of America; Optics Express; 23; 23; 11-2015; 29772-297781094-4087CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1364/OE.23.029772info:eu-repo/semantics/altIdentifier/url/https://www.osapublishing.org/oe/abstract.cfm?uri=oe-23-23-29772info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:09:46Zoai:ri.conicet.gov.ar:11336/46232instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:09:46.88CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Improving image contrast in fluorescence microscopy with nanostructured substrates |
title |
Improving image contrast in fluorescence microscopy with nanostructured substrates |
spellingShingle |
Improving image contrast in fluorescence microscopy with nanostructured substrates Brunstein, Maia FLUORESCENCE MICROSCOPY ILLUMINATION DESIGN RESONATORS |
title_short |
Improving image contrast in fluorescence microscopy with nanostructured substrates |
title_full |
Improving image contrast in fluorescence microscopy with nanostructured substrates |
title_fullStr |
Improving image contrast in fluorescence microscopy with nanostructured substrates |
title_full_unstemmed |
Improving image contrast in fluorescence microscopy with nanostructured substrates |
title_sort |
Improving image contrast in fluorescence microscopy with nanostructured substrates |
dc.creator.none.fl_str_mv |
Brunstein, Maia Cattoni, Andrea Estrada, Laura Cecilia Yacomotti, Alejandro M. |
author |
Brunstein, Maia |
author_facet |
Brunstein, Maia Cattoni, Andrea Estrada, Laura Cecilia Yacomotti, Alejandro M. |
author_role |
author |
author2 |
Cattoni, Andrea Estrada, Laura Cecilia Yacomotti, Alejandro M. |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
FLUORESCENCE MICROSCOPY ILLUMINATION DESIGN RESONATORS |
topic |
FLUORESCENCE MICROSCOPY ILLUMINATION DESIGN RESONATORS |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.10 https://purl.org/becyt/ford/2 |
dc.description.none.fl_txt_mv |
Metallic and dielectric nanostructures can show sharp contrastedresonances, sensitive to the environment, and high field enhancement insub-wavelength volumes. For this reason, these structures are commonlyused as molecular sensors. Only few works have focused on theirapplication in optical microscopy, in particular in superresolution. In thiswork we have designed, fabricated and optically tested a nanostructuredTiO2 substrate, fabricated by direct embossing of TiO2 derived film, as asubstrate for fluorescence microscopy. Moreover, using numericalsimulations, we have compared the signal to background noise with respectto other metallo-dielectric structures. We show that the TiO2 structure is agood candidate for reducing the thickness of the fluorescence excitationdown to ~100 nm. Therefore, this substrate can be used to obtain TotalInternal Reflection (TIRF) axial resolution without a TIRF-Microscopysystem. Fil: Brunstein, Maia. Centre National de la Recherche Scientifique; Francia. Université Paris-Saclay; Francia Fil: Cattoni, Andrea. Centre National de la Recherche Scientifique; Francia. Université Paris-Saclay; Francia Fil: Estrada, Laura Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina Fil: Yacomotti, Alejandro M.. Centre National de la Recherche Scientifique; Francia. Université Paris-Saclay; Francia |
description |
Metallic and dielectric nanostructures can show sharp contrastedresonances, sensitive to the environment, and high field enhancement insub-wavelength volumes. For this reason, these structures are commonlyused as molecular sensors. Only few works have focused on theirapplication in optical microscopy, in particular in superresolution. In thiswork we have designed, fabricated and optically tested a nanostructuredTiO2 substrate, fabricated by direct embossing of TiO2 derived film, as asubstrate for fluorescence microscopy. Moreover, using numericalsimulations, we have compared the signal to background noise with respectto other metallo-dielectric structures. We show that the TiO2 structure is agood candidate for reducing the thickness of the fluorescence excitationdown to ~100 nm. Therefore, this substrate can be used to obtain TotalInternal Reflection (TIRF) axial resolution without a TIRF-Microscopysystem. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-11 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/46232 Brunstein, Maia; Cattoni, Andrea; Estrada, Laura Cecilia; Yacomotti, Alejandro M.; Improving image contrast in fluorescence microscopy with nanostructured substrates; Optical Society of America; Optics Express; 23; 23; 11-2015; 29772-29778 1094-4087 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/46232 |
identifier_str_mv |
Brunstein, Maia; Cattoni, Andrea; Estrada, Laura Cecilia; Yacomotti, Alejandro M.; Improving image contrast in fluorescence microscopy with nanostructured substrates; Optical Society of America; Optics Express; 23; 23; 11-2015; 29772-29778 1094-4087 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1364/OE.23.029772 info:eu-repo/semantics/altIdentifier/url/https://www.osapublishing.org/oe/abstract.cfm?uri=oe-23-23-29772 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Optical Society of America |
publisher.none.fl_str_mv |
Optical Society of America |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1846083245574193152 |
score |
13.22299 |