Improving image contrast in fluorescence microscopy with nanostructured substrates

Autores
Brunstein, Maia; Cattoni, Andrea; Estrada, Laura Cecilia; Yacomotti, Alejandro M.
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Metallic and dielectric nanostructures can show sharp contrastedresonances, sensitive to the environment, and high field enhancement insub-wavelength volumes. For this reason, these structures are commonlyused as molecular sensors. Only few works have focused on theirapplication in optical microscopy, in particular in superresolution. In thiswork we have designed, fabricated and optically tested a nanostructuredTiO2 substrate, fabricated by direct embossing of TiO2 derived film, as asubstrate for fluorescence microscopy. Moreover, using numericalsimulations, we have compared the signal to background noise with respectto other metallo-dielectric structures. We show that the TiO2 structure is agood candidate for reducing the thickness of the fluorescence excitationdown to ~100 nm. Therefore, this substrate can be used to obtain TotalInternal Reflection (TIRF) axial resolution without a TIRF-Microscopysystem.
Fil: Brunstein, Maia. Centre National de la Recherche Scientifique; Francia. Université Paris-Saclay; Francia
Fil: Cattoni, Andrea. Centre National de la Recherche Scientifique; Francia. Université Paris-Saclay; Francia
Fil: Estrada, Laura Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
Fil: Yacomotti, Alejandro M.. Centre National de la Recherche Scientifique; Francia. Université Paris-Saclay; Francia
Materia
FLUORESCENCE MICROSCOPY
ILLUMINATION DESIGN
RESONATORS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/46232

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network_name_str CONICET Digital (CONICET)
spelling Improving image contrast in fluorescence microscopy with nanostructured substratesBrunstein, MaiaCattoni, AndreaEstrada, Laura CeciliaYacomotti, Alejandro M.FLUORESCENCE MICROSCOPYILLUMINATION DESIGNRESONATORShttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2Metallic and dielectric nanostructures can show sharp contrastedresonances, sensitive to the environment, and high field enhancement insub-wavelength volumes. For this reason, these structures are commonlyused as molecular sensors. Only few works have focused on theirapplication in optical microscopy, in particular in superresolution. In thiswork we have designed, fabricated and optically tested a nanostructuredTiO2 substrate, fabricated by direct embossing of TiO2 derived film, as asubstrate for fluorescence microscopy. Moreover, using numericalsimulations, we have compared the signal to background noise with respectto other metallo-dielectric structures. We show that the TiO2 structure is agood candidate for reducing the thickness of the fluorescence excitationdown to ~100 nm. Therefore, this substrate can be used to obtain TotalInternal Reflection (TIRF) axial resolution without a TIRF-Microscopysystem.Fil: Brunstein, Maia. Centre National de la Recherche Scientifique; Francia. Université Paris-Saclay; FranciaFil: Cattoni, Andrea. Centre National de la Recherche Scientifique; Francia. Université Paris-Saclay; FranciaFil: Estrada, Laura Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; ArgentinaFil: Yacomotti, Alejandro M.. Centre National de la Recherche Scientifique; Francia. Université Paris-Saclay; FranciaOptical Society of America2015-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/46232Brunstein, Maia; Cattoni, Andrea; Estrada, Laura Cecilia; Yacomotti, Alejandro M.; Improving image contrast in fluorescence microscopy with nanostructured substrates; Optical Society of America; Optics Express; 23; 23; 11-2015; 29772-297781094-4087CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1364/OE.23.029772info:eu-repo/semantics/altIdentifier/url/https://www.osapublishing.org/oe/abstract.cfm?uri=oe-23-23-29772info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:09:46Zoai:ri.conicet.gov.ar:11336/46232instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:09:46.88CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Improving image contrast in fluorescence microscopy with nanostructured substrates
title Improving image contrast in fluorescence microscopy with nanostructured substrates
spellingShingle Improving image contrast in fluorescence microscopy with nanostructured substrates
Brunstein, Maia
FLUORESCENCE MICROSCOPY
ILLUMINATION DESIGN
RESONATORS
title_short Improving image contrast in fluorescence microscopy with nanostructured substrates
title_full Improving image contrast in fluorescence microscopy with nanostructured substrates
title_fullStr Improving image contrast in fluorescence microscopy with nanostructured substrates
title_full_unstemmed Improving image contrast in fluorescence microscopy with nanostructured substrates
title_sort Improving image contrast in fluorescence microscopy with nanostructured substrates
dc.creator.none.fl_str_mv Brunstein, Maia
Cattoni, Andrea
Estrada, Laura Cecilia
Yacomotti, Alejandro M.
author Brunstein, Maia
author_facet Brunstein, Maia
Cattoni, Andrea
Estrada, Laura Cecilia
Yacomotti, Alejandro M.
author_role author
author2 Cattoni, Andrea
Estrada, Laura Cecilia
Yacomotti, Alejandro M.
author2_role author
author
author
dc.subject.none.fl_str_mv FLUORESCENCE MICROSCOPY
ILLUMINATION DESIGN
RESONATORS
topic FLUORESCENCE MICROSCOPY
ILLUMINATION DESIGN
RESONATORS
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Metallic and dielectric nanostructures can show sharp contrastedresonances, sensitive to the environment, and high field enhancement insub-wavelength volumes. For this reason, these structures are commonlyused as molecular sensors. Only few works have focused on theirapplication in optical microscopy, in particular in superresolution. In thiswork we have designed, fabricated and optically tested a nanostructuredTiO2 substrate, fabricated by direct embossing of TiO2 derived film, as asubstrate for fluorescence microscopy. Moreover, using numericalsimulations, we have compared the signal to background noise with respectto other metallo-dielectric structures. We show that the TiO2 structure is agood candidate for reducing the thickness of the fluorescence excitationdown to ~100 nm. Therefore, this substrate can be used to obtain TotalInternal Reflection (TIRF) axial resolution without a TIRF-Microscopysystem.
Fil: Brunstein, Maia. Centre National de la Recherche Scientifique; Francia. Université Paris-Saclay; Francia
Fil: Cattoni, Andrea. Centre National de la Recherche Scientifique; Francia. Université Paris-Saclay; Francia
Fil: Estrada, Laura Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
Fil: Yacomotti, Alejandro M.. Centre National de la Recherche Scientifique; Francia. Université Paris-Saclay; Francia
description Metallic and dielectric nanostructures can show sharp contrastedresonances, sensitive to the environment, and high field enhancement insub-wavelength volumes. For this reason, these structures are commonlyused as molecular sensors. Only few works have focused on theirapplication in optical microscopy, in particular in superresolution. In thiswork we have designed, fabricated and optically tested a nanostructuredTiO2 substrate, fabricated by direct embossing of TiO2 derived film, as asubstrate for fluorescence microscopy. Moreover, using numericalsimulations, we have compared the signal to background noise with respectto other metallo-dielectric structures. We show that the TiO2 structure is agood candidate for reducing the thickness of the fluorescence excitationdown to ~100 nm. Therefore, this substrate can be used to obtain TotalInternal Reflection (TIRF) axial resolution without a TIRF-Microscopysystem.
publishDate 2015
dc.date.none.fl_str_mv 2015-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/46232
Brunstein, Maia; Cattoni, Andrea; Estrada, Laura Cecilia; Yacomotti, Alejandro M.; Improving image contrast in fluorescence microscopy with nanostructured substrates; Optical Society of America; Optics Express; 23; 23; 11-2015; 29772-29778
1094-4087
CONICET Digital
CONICET
url http://hdl.handle.net/11336/46232
identifier_str_mv Brunstein, Maia; Cattoni, Andrea; Estrada, Laura Cecilia; Yacomotti, Alejandro M.; Improving image contrast in fluorescence microscopy with nanostructured substrates; Optical Society of America; Optics Express; 23; 23; 11-2015; 29772-29778
1094-4087
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1364/OE.23.029772
info:eu-repo/semantics/altIdentifier/url/https://www.osapublishing.org/oe/abstract.cfm?uri=oe-23-23-29772
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Optical Society of America
publisher.none.fl_str_mv Optical Society of America
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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