Tumor extracellular matrix: lessons from the second-harmonic generation microscopy

Autores
de Andrade Natal, Rodrigo; Adur, Javier Fernando; Lenz Cesar, Carlos; Vassallo, José
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Extracellular matrix (ECM) represents more than a mere intercellular cement. It is physiologically active in cell communication, adhesion and proliferation. Collagen is the most abundant protein, making up to 90% of ECM, and 30% of total protein weight in humans. Second-harmonic generation (SHG) microscopy represents an important tool to study collagen organization of ECM in freshly unfixed tissues and paraffin-embedded tissue samples. This manuscript aims to review some of the applications of SHG microscopy in Oncologic Pathology, mainly in the study of ECM of epithelial tumors. It is shown how collagen parameters measured by this technique can aid in the differential diagnosis and in prognostic stratification. There is a tendency to associate higher amount, lower organization and higher linearity of collagen fibers with tumor progression and metastasizing. These represent complex processes, in which matrix remodeling plays a central role, together with cancer cell genetic modifications. Integration of studies on cancer cell biology and ECM are highly advantageous to give us a more complete picture of these processes. As microscopic techniques provide topographic information allied with biologic characteristics of tissue components, they represent important tools for a more complete understanding of cancer progression. In this context, SHG has provided significant insights in human tumor specimens, readily available for Pathologists.
Fil: de Andrade Natal, Rodrigo. Universidade Estadual de Campinas; Brasil
Fil: Adur, Javier Fernando. Universidad Nacional de Entre Ríos. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática; Argentina
Fil: Lenz Cesar, Carlos. Universidade Estadual de Campinas; Brasil
Fil: Vassallo, José. Universidade Estadual de Campinas; Brasil
Materia
Matriz Extracelular
Cáncer
Microscopia No lineal
SHG
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/173415
Acceder
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spelling Tumor extracellular matrix: lessons from the second-harmonic generation microscopyde Andrade Natal, RodrigoAdur, Javier FernandoLenz Cesar, CarlosVassallo, JoséMatriz ExtracelularCáncerMicroscopia No linealSHGhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Extracellular matrix (ECM) represents more than a mere intercellular cement. It is physiologically active in cell communication, adhesion and proliferation. Collagen is the most abundant protein, making up to 90% of ECM, and 30% of total protein weight in humans. Second-harmonic generation (SHG) microscopy represents an important tool to study collagen organization of ECM in freshly unfixed tissues and paraffin-embedded tissue samples. This manuscript aims to review some of the applications of SHG microscopy in Oncologic Pathology, mainly in the study of ECM of epithelial tumors. It is shown how collagen parameters measured by this technique can aid in the differential diagnosis and in prognostic stratification. There is a tendency to associate higher amount, lower organization and higher linearity of collagen fibers with tumor progression and metastasizing. These represent complex processes, in which matrix remodeling plays a central role, together with cancer cell genetic modifications. Integration of studies on cancer cell biology and ECM are highly advantageous to give us a more complete picture of these processes. As microscopic techniques provide topographic information allied with biologic characteristics of tissue components, they represent important tools for a more complete understanding of cancer progression. In this context, SHG has provided significant insights in human tumor specimens, readily available for Pathologists.Fil: de Andrade Natal, Rodrigo. Universidade Estadual de Campinas; BrasilFil: Adur, Javier Fernando. Universidad Nacional de Entre Ríos. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática; ArgentinaFil: Lenz Cesar, Carlos. Universidade Estadual de Campinas; BrasilFil: Vassallo, José. Universidade Estadual de Campinas; BrasilSpringer2021-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/173415de Andrade Natal, Rodrigo; Adur, Javier Fernando; Lenz Cesar, Carlos; Vassallo, José; Tumor extracellular matrix: lessons from the second-harmonic generation microscopy; Springer; Surgical and Experimental Pathology; 4; 1; 3-2021; 1-122520-8454CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1186/s42047-021-00089-0info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:43:37Zoai:ri.conicet.gov.ar:11336/173415instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:43:38.095CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Tumor extracellular matrix: lessons from the second-harmonic generation microscopy
title Tumor extracellular matrix: lessons from the second-harmonic generation microscopy
spellingShingle Tumor extracellular matrix: lessons from the second-harmonic generation microscopy
de Andrade Natal, Rodrigo
Matriz Extracelular
Cáncer
Microscopia No lineal
SHG
title_short Tumor extracellular matrix: lessons from the second-harmonic generation microscopy
title_full Tumor extracellular matrix: lessons from the second-harmonic generation microscopy
title_fullStr Tumor extracellular matrix: lessons from the second-harmonic generation microscopy
title_full_unstemmed Tumor extracellular matrix: lessons from the second-harmonic generation microscopy
title_sort Tumor extracellular matrix: lessons from the second-harmonic generation microscopy
dc.creator.none.fl_str_mv de Andrade Natal, Rodrigo
Adur, Javier Fernando
Lenz Cesar, Carlos
Vassallo, José
author de Andrade Natal, Rodrigo
author_facet de Andrade Natal, Rodrigo
Adur, Javier Fernando
Lenz Cesar, Carlos
Vassallo, José
author_role author
author2 Adur, Javier Fernando
Lenz Cesar, Carlos
Vassallo, José
author2_role author
author
author
dc.subject.none.fl_str_mv Matriz Extracelular
Cáncer
Microscopia No lineal
SHG
topic Matriz Extracelular
Cáncer
Microscopia No lineal
SHG
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Extracellular matrix (ECM) represents more than a mere intercellular cement. It is physiologically active in cell communication, adhesion and proliferation. Collagen is the most abundant protein, making up to 90% of ECM, and 30% of total protein weight in humans. Second-harmonic generation (SHG) microscopy represents an important tool to study collagen organization of ECM in freshly unfixed tissues and paraffin-embedded tissue samples. This manuscript aims to review some of the applications of SHG microscopy in Oncologic Pathology, mainly in the study of ECM of epithelial tumors. It is shown how collagen parameters measured by this technique can aid in the differential diagnosis and in prognostic stratification. There is a tendency to associate higher amount, lower organization and higher linearity of collagen fibers with tumor progression and metastasizing. These represent complex processes, in which matrix remodeling plays a central role, together with cancer cell genetic modifications. Integration of studies on cancer cell biology and ECM are highly advantageous to give us a more complete picture of these processes. As microscopic techniques provide topographic information allied with biologic characteristics of tissue components, they represent important tools for a more complete understanding of cancer progression. In this context, SHG has provided significant insights in human tumor specimens, readily available for Pathologists.
Fil: de Andrade Natal, Rodrigo. Universidade Estadual de Campinas; Brasil
Fil: Adur, Javier Fernando. Universidad Nacional de Entre Ríos. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática; Argentina
Fil: Lenz Cesar, Carlos. Universidade Estadual de Campinas; Brasil
Fil: Vassallo, José. Universidade Estadual de Campinas; Brasil
description Extracellular matrix (ECM) represents more than a mere intercellular cement. It is physiologically active in cell communication, adhesion and proliferation. Collagen is the most abundant protein, making up to 90% of ECM, and 30% of total protein weight in humans. Second-harmonic generation (SHG) microscopy represents an important tool to study collagen organization of ECM in freshly unfixed tissues and paraffin-embedded tissue samples. This manuscript aims to review some of the applications of SHG microscopy in Oncologic Pathology, mainly in the study of ECM of epithelial tumors. It is shown how collagen parameters measured by this technique can aid in the differential diagnosis and in prognostic stratification. There is a tendency to associate higher amount, lower organization and higher linearity of collagen fibers with tumor progression and metastasizing. These represent complex processes, in which matrix remodeling plays a central role, together with cancer cell genetic modifications. Integration of studies on cancer cell biology and ECM are highly advantageous to give us a more complete picture of these processes. As microscopic techniques provide topographic information allied with biologic characteristics of tissue components, they represent important tools for a more complete understanding of cancer progression. In this context, SHG has provided significant insights in human tumor specimens, readily available for Pathologists.
publishDate 2021
dc.date.none.fl_str_mv 2021-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/173415
de Andrade Natal, Rodrigo; Adur, Javier Fernando; Lenz Cesar, Carlos; Vassallo, José; Tumor extracellular matrix: lessons from the second-harmonic generation microscopy; Springer; Surgical and Experimental Pathology; 4; 1; 3-2021; 1-12
2520-8454
CONICET Digital
CONICET
url http://hdl.handle.net/11336/173415
identifier_str_mv de Andrade Natal, Rodrigo; Adur, Javier Fernando; Lenz Cesar, Carlos; Vassallo, José; Tumor extracellular matrix: lessons from the second-harmonic generation microscopy; Springer; Surgical and Experimental Pathology; 4; 1; 3-2021; 1-12
2520-8454
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1186/s42047-021-00089-0
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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