Comparison of morphological changes of corneal collagen fibers treated with collagen crosslinking agents using second harmonic generation images

Autores
Zeitoune, Angel Alberto; Bersanetti, Patrícia A.; Schor, Paulo; Erbes, Luciana Ariadna; Cesar, Carlos L.; Adur, Javier Fernando
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Corneal cross-linking (CXL) is a common surgical procedure used to modify corneal biomechanics and stabilize keratoconus progression which is still under discussion. Its side effects, which are mostly related to anatomical unpredictability and stromal exposure, are the reason for the search for new CXL agents. In this work we have quantitatively evaluated the porcine corneal stroma architecture treated with collagen crosslinking agents such as riboflavin solutions and açai extract, using second harmonic generation microscopy. Aimed at evaluating the morphological changes in the corneal stroma after collagen crosslinking under a CXL chemical agent, a tubeness filter based Hessian matrix to obtain a 3D fiber characterization of the SHG images was applied. The results showed a curling effect and shortening of the collagen fibers treated with açai as compared to the control. They also showed a higher degree of clustering of the collagen fibers with larger empty spaces when compared to the other two groups. We believe that studies such as these presented in this paper are a good direct nondestructive and free labeling evaluation technique that allows the observation of morphologic features of corneas treated with new CXL agents.
Fil: Zeitoune, Angel Alberto. Universidad Nacional de Entre Ríos. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática; Argentina
Fil: Bersanetti, Patrícia A.. Universidade Federal de Sao Paulo; Brasil
Fil: Schor, Paulo. Universidade Federal de Sao Paulo; Brasil
Fil: Erbes, Luciana Ariadna. Universidad Nacional de Entre Ríos. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática; Argentina
Fil: Cesar, Carlos L.. Instituto Nacional de Fotônica Aplicada à Biologia Celular; Brasil. Universidade Federal do Ceara; Brasil
Fil: Adur, Javier Fernando. Universidad Nacional de Entre Ríos. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática; Argentina
Materia
COLLAGEN
CROSSLINKING
SHG (SECOND HARMONIC GENERATION)
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/142916
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/142916
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Comparison of morphological changes of corneal collagen fibers treated with collagen crosslinking agents using second harmonic generation imagesZeitoune, Angel AlbertoBersanetti, Patrícia A.Schor, PauloErbes, Luciana AriadnaCesar, Carlos L.Adur, Javier FernandoCOLLAGENCROSSLINKINGSHG (SECOND HARMONIC GENERATION)https://purl.org/becyt/ford/1.7https://purl.org/becyt/ford/1Corneal cross-linking (CXL) is a common surgical procedure used to modify corneal biomechanics and stabilize keratoconus progression which is still under discussion. Its side effects, which are mostly related to anatomical unpredictability and stromal exposure, are the reason for the search for new CXL agents. In this work we have quantitatively evaluated the porcine corneal stroma architecture treated with collagen crosslinking agents such as riboflavin solutions and açai extract, using second harmonic generation microscopy. Aimed at evaluating the morphological changes in the corneal stroma after collagen crosslinking under a CXL chemical agent, a tubeness filter based Hessian matrix to obtain a 3D fiber characterization of the SHG images was applied. The results showed a curling effect and shortening of the collagen fibers treated with açai as compared to the control. They also showed a higher degree of clustering of the collagen fibers with larger empty spaces when compared to the other two groups. We believe that studies such as these presented in this paper are a good direct nondestructive and free labeling evaluation technique that allows the observation of morphologic features of corneas treated with new CXL agents.Fil: Zeitoune, Angel Alberto. Universidad Nacional de Entre Ríos. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática; ArgentinaFil: Bersanetti, Patrícia A.. Universidade Federal de Sao Paulo; BrasilFil: Schor, Paulo. Universidade Federal de Sao Paulo; BrasilFil: Erbes, Luciana Ariadna. Universidad Nacional de Entre Ríos. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática; ArgentinaFil: Cesar, Carlos L.. Instituto Nacional de Fotônica Aplicada à Biologia Celular; Brasil. Universidade Federal do Ceara; BrasilFil: Adur, Javier Fernando. Universidad Nacional de Entre Ríos. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática; ArgentinaElsevier Science2020-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/142916Zeitoune, Angel Alberto; Bersanetti, Patrícia A.; Schor, Paulo; Erbes, Luciana Ariadna; Cesar, Carlos L.; et al.; Comparison of morphological changes of corneal collagen fibers treated with collagen crosslinking agents using second harmonic generation images; Elsevier Science; International Journal of Biological Macromolecules; 165; 12-2020; 346-3530141-8130CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijbiomac.2020.09.147info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0141813020345062info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:05:44Zoai:ri.conicet.gov.ar:11336/142916instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:05:44.591CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Comparison of morphological changes of corneal collagen fibers treated with collagen crosslinking agents using second harmonic generation images
title Comparison of morphological changes of corneal collagen fibers treated with collagen crosslinking agents using second harmonic generation images
spellingShingle Comparison of morphological changes of corneal collagen fibers treated with collagen crosslinking agents using second harmonic generation images
Zeitoune, Angel Alberto
COLLAGEN
CROSSLINKING
SHG (SECOND HARMONIC GENERATION)
title_short Comparison of morphological changes of corneal collagen fibers treated with collagen crosslinking agents using second harmonic generation images
title_full Comparison of morphological changes of corneal collagen fibers treated with collagen crosslinking agents using second harmonic generation images
title_fullStr Comparison of morphological changes of corneal collagen fibers treated with collagen crosslinking agents using second harmonic generation images
title_full_unstemmed Comparison of morphological changes of corneal collagen fibers treated with collagen crosslinking agents using second harmonic generation images
title_sort Comparison of morphological changes of corneal collagen fibers treated with collagen crosslinking agents using second harmonic generation images
dc.creator.none.fl_str_mv Zeitoune, Angel Alberto
Bersanetti, Patrícia A.
Schor, Paulo
Erbes, Luciana Ariadna
Cesar, Carlos L.
Adur, Javier Fernando
author Zeitoune, Angel Alberto
author_facet Zeitoune, Angel Alberto
Bersanetti, Patrícia A.
Schor, Paulo
Erbes, Luciana Ariadna
Cesar, Carlos L.
Adur, Javier Fernando
author_role author
author2 Bersanetti, Patrícia A.
Schor, Paulo
Erbes, Luciana Ariadna
Cesar, Carlos L.
Adur, Javier Fernando
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv COLLAGEN
CROSSLINKING
SHG (SECOND HARMONIC GENERATION)
topic COLLAGEN
CROSSLINKING
SHG (SECOND HARMONIC GENERATION)
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.7
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Corneal cross-linking (CXL) is a common surgical procedure used to modify corneal biomechanics and stabilize keratoconus progression which is still under discussion. Its side effects, which are mostly related to anatomical unpredictability and stromal exposure, are the reason for the search for new CXL agents. In this work we have quantitatively evaluated the porcine corneal stroma architecture treated with collagen crosslinking agents such as riboflavin solutions and açai extract, using second harmonic generation microscopy. Aimed at evaluating the morphological changes in the corneal stroma after collagen crosslinking under a CXL chemical agent, a tubeness filter based Hessian matrix to obtain a 3D fiber characterization of the SHG images was applied. The results showed a curling effect and shortening of the collagen fibers treated with açai as compared to the control. They also showed a higher degree of clustering of the collagen fibers with larger empty spaces when compared to the other two groups. We believe that studies such as these presented in this paper are a good direct nondestructive and free labeling evaluation technique that allows the observation of morphologic features of corneas treated with new CXL agents.
Fil: Zeitoune, Angel Alberto. Universidad Nacional de Entre Ríos. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática; Argentina
Fil: Bersanetti, Patrícia A.. Universidade Federal de Sao Paulo; Brasil
Fil: Schor, Paulo. Universidade Federal de Sao Paulo; Brasil
Fil: Erbes, Luciana Ariadna. Universidad Nacional de Entre Ríos. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática; Argentina
Fil: Cesar, Carlos L.. Instituto Nacional de Fotônica Aplicada à Biologia Celular; Brasil. Universidade Federal do Ceara; Brasil
Fil: Adur, Javier Fernando. Universidad Nacional de Entre Ríos. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática; Argentina
description Corneal cross-linking (CXL) is a common surgical procedure used to modify corneal biomechanics and stabilize keratoconus progression which is still under discussion. Its side effects, which are mostly related to anatomical unpredictability and stromal exposure, are the reason for the search for new CXL agents. In this work we have quantitatively evaluated the porcine corneal stroma architecture treated with collagen crosslinking agents such as riboflavin solutions and açai extract, using second harmonic generation microscopy. Aimed at evaluating the morphological changes in the corneal stroma after collagen crosslinking under a CXL chemical agent, a tubeness filter based Hessian matrix to obtain a 3D fiber characterization of the SHG images was applied. The results showed a curling effect and shortening of the collagen fibers treated with açai as compared to the control. They also showed a higher degree of clustering of the collagen fibers with larger empty spaces when compared to the other two groups. We believe that studies such as these presented in this paper are a good direct nondestructive and free labeling evaluation technique that allows the observation of morphologic features of corneas treated with new CXL agents.
publishDate 2020
dc.date.none.fl_str_mv 2020-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/142916
Zeitoune, Angel Alberto; Bersanetti, Patrícia A.; Schor, Paulo; Erbes, Luciana Ariadna; Cesar, Carlos L.; et al.; Comparison of morphological changes of corneal collagen fibers treated with collagen crosslinking agents using second harmonic generation images; Elsevier Science; International Journal of Biological Macromolecules; 165; 12-2020; 346-353
0141-8130
CONICET Digital
CONICET
url http://hdl.handle.net/11336/142916
identifier_str_mv Zeitoune, Angel Alberto; Bersanetti, Patrícia A.; Schor, Paulo; Erbes, Luciana Ariadna; Cesar, Carlos L.; et al.; Comparison of morphological changes of corneal collagen fibers treated with collagen crosslinking agents using second harmonic generation images; Elsevier Science; International Journal of Biological Macromolecules; 165; 12-2020; 346-353
0141-8130
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijbiomac.2020.09.147
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0141813020345062
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613897178316800
score 13.070432

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