Bacterial Isolates from Ethiopian Soda Lake Producers of Alkaline-Active β-Glucanases Resistant to Chelating and Surfactant Compounds

Autores
Minig, Paola Marisol; Walker, D.; Ledesma, Julian Natan; Martinez, Maria Alejandra; Breccia, Javier Dario
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
β-glucanase activities were screened from isolated bacteria of the Ethiopian Shala Lake. Five isolates were selected according to the highest production of alkaline-active β-glucanase. By sequence analysis of 16S rDNA and physiological tests, four strains (SES01, SES22, SES4 and SES05) were related to Bacillus halodurans specie and the strain SES33 was identified up to genus level as Bacillus sp. Intergenic spacer regions fingerprinting showed different patterns among selected strains, having DNA amplicons of high molecular weight characteristic of alkaliphilic Bacillus. Herein, B. halodurans SES01 produced a highly stable β-glucanase in presence of surfactant and chelating compounds (sodium lauryl sulphate, Triton X-100 and EDTA) indicating its potential as additive for laundry technologies.
Fil: Minig, Paola Marisol. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina
Fil: Walker, D.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Ledesma, Julian Natan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Martinez, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Breccia, Javier Dario. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina
Materia
Bacillus halodurans
Beta glucanase
Surfactants
EDTA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/45863
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Bacterial Isolates from Ethiopian Soda Lake Producers of Alkaline-Active β-Glucanases Resistant to Chelating and Surfactant CompoundsMinig, Paola MarisolWalker, D.Ledesma, Julian NatanMartinez, Maria AlejandraBreccia, Javier DarioBacillus haloduransBeta glucanaseSurfactantsEDTAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1β-glucanase activities were screened from isolated bacteria of the Ethiopian Shala Lake. Five isolates were selected according to the highest production of alkaline-active β-glucanase. By sequence analysis of 16S rDNA and physiological tests, four strains (SES01, SES22, SES4 and SES05) were related to Bacillus halodurans specie and the strain SES33 was identified up to genus level as Bacillus sp. Intergenic spacer regions fingerprinting showed different patterns among selected strains, having DNA amplicons of high molecular weight characteristic of alkaliphilic Bacillus. Herein, B. halodurans SES01 produced a highly stable β-glucanase in presence of surfactant and chelating compounds (sodium lauryl sulphate, Triton X-100 and EDTA) indicating its potential as additive for laundry technologies.Fil: Minig, Paola Marisol. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Departamento de Química; ArgentinaFil: Walker, D.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Ledesma, Julian Natan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Martinez, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Breccia, Javier Dario. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Departamento de Química; ArgentinaAcademic Journals2009-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/45863Minig, Paola Marisol; Walker, D.; Ledesma, Julian Natan; Martinez, Maria Alejandra; Breccia, Javier Dario; Bacterial Isolates from Ethiopian Soda Lake Producers of Alkaline-Active β-Glucanases Resistant to Chelating and Surfactant Compounds; Academic Journals; Research Journal of Microbiology; 4; 5; 5-2009; 194-2011816-4935CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3923/jm.2009.194.201info:eu-repo/semantics/altIdentifier/url/https://scialert.net/abstract/?doi=jm.2009.194.201info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:06:22Zoai:ri.conicet.gov.ar:11336/45863instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:06:22.839CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Bacterial Isolates from Ethiopian Soda Lake Producers of Alkaline-Active β-Glucanases Resistant to Chelating and Surfactant Compounds
title Bacterial Isolates from Ethiopian Soda Lake Producers of Alkaline-Active β-Glucanases Resistant to Chelating and Surfactant Compounds
spellingShingle Bacterial Isolates from Ethiopian Soda Lake Producers of Alkaline-Active β-Glucanases Resistant to Chelating and Surfactant Compounds
Minig, Paola Marisol
Bacillus halodurans
Beta glucanase
Surfactants
EDTA
title_short Bacterial Isolates from Ethiopian Soda Lake Producers of Alkaline-Active β-Glucanases Resistant to Chelating and Surfactant Compounds
title_full Bacterial Isolates from Ethiopian Soda Lake Producers of Alkaline-Active β-Glucanases Resistant to Chelating and Surfactant Compounds
title_fullStr Bacterial Isolates from Ethiopian Soda Lake Producers of Alkaline-Active β-Glucanases Resistant to Chelating and Surfactant Compounds
title_full_unstemmed Bacterial Isolates from Ethiopian Soda Lake Producers of Alkaline-Active β-Glucanases Resistant to Chelating and Surfactant Compounds
title_sort Bacterial Isolates from Ethiopian Soda Lake Producers of Alkaline-Active β-Glucanases Resistant to Chelating and Surfactant Compounds
dc.creator.none.fl_str_mv Minig, Paola Marisol
Walker, D.
Ledesma, Julian Natan
Martinez, Maria Alejandra
Breccia, Javier Dario
author Minig, Paola Marisol
author_facet Minig, Paola Marisol
Walker, D.
Ledesma, Julian Natan
Martinez, Maria Alejandra
Breccia, Javier Dario
author_role author
author2 Walker, D.
Ledesma, Julian Natan
Martinez, Maria Alejandra
Breccia, Javier Dario
author2_role author
author
author
author
dc.subject.none.fl_str_mv Bacillus halodurans
Beta glucanase
Surfactants
EDTA
topic Bacillus halodurans
Beta glucanase
Surfactants
EDTA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv β-glucanase activities were screened from isolated bacteria of the Ethiopian Shala Lake. Five isolates were selected according to the highest production of alkaline-active β-glucanase. By sequence analysis of 16S rDNA and physiological tests, four strains (SES01, SES22, SES4 and SES05) were related to Bacillus halodurans specie and the strain SES33 was identified up to genus level as Bacillus sp. Intergenic spacer regions fingerprinting showed different patterns among selected strains, having DNA amplicons of high molecular weight characteristic of alkaliphilic Bacillus. Herein, B. halodurans SES01 produced a highly stable β-glucanase in presence of surfactant and chelating compounds (sodium lauryl sulphate, Triton X-100 and EDTA) indicating its potential as additive for laundry technologies.
Fil: Minig, Paola Marisol. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina
Fil: Walker, D.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Ledesma, Julian Natan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Martinez, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Breccia, Javier Dario. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina
description β-glucanase activities were screened from isolated bacteria of the Ethiopian Shala Lake. Five isolates were selected according to the highest production of alkaline-active β-glucanase. By sequence analysis of 16S rDNA and physiological tests, four strains (SES01, SES22, SES4 and SES05) were related to Bacillus halodurans specie and the strain SES33 was identified up to genus level as Bacillus sp. Intergenic spacer regions fingerprinting showed different patterns among selected strains, having DNA amplicons of high molecular weight characteristic of alkaliphilic Bacillus. Herein, B. halodurans SES01 produced a highly stable β-glucanase in presence of surfactant and chelating compounds (sodium lauryl sulphate, Triton X-100 and EDTA) indicating its potential as additive for laundry technologies.
publishDate 2009
dc.date.none.fl_str_mv 2009-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/45863
Minig, Paola Marisol; Walker, D.; Ledesma, Julian Natan; Martinez, Maria Alejandra; Breccia, Javier Dario; Bacterial Isolates from Ethiopian Soda Lake Producers of Alkaline-Active β-Glucanases Resistant to Chelating and Surfactant Compounds; Academic Journals; Research Journal of Microbiology; 4; 5; 5-2009; 194-201
1816-4935
CONICET Digital
CONICET
url http://hdl.handle.net/11336/45863
identifier_str_mv Minig, Paola Marisol; Walker, D.; Ledesma, Julian Natan; Martinez, Maria Alejandra; Breccia, Javier Dario; Bacterial Isolates from Ethiopian Soda Lake Producers of Alkaline-Active β-Glucanases Resistant to Chelating and Surfactant Compounds; Academic Journals; Research Journal of Microbiology; 4; 5; 5-2009; 194-201
1816-4935
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3923/jm.2009.194.201
info:eu-repo/semantics/altIdentifier/url/https://scialert.net/abstract/?doi=jm.2009.194.201
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Journals
publisher.none.fl_str_mv Academic Journals
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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