Regarding the effect that different hydrocarbon/fluorocarbon surfactant mixtures have on their complexation with HSA

Autores
Blanco, Elena; Messina, Paula Verónica; Ruso, Juan M.; Prieto, Gerardo; Sarmiento, Félix
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The complexations between human serum albumin (HSA) and the sodium perfluorooctanoate/sodium octanoate and sodium perfluorooctanoate/sodium dodecanoate systems have been studied by a combination of electrical conductivity, ion-selective electrode, electrophoresis, and spectroscopy measurements. The binary mixtures of the surfactants deviated slightly from ideality. Binding plots revealed the existence of two specific binding sites, the first site being more accessible than the second. Positive cooperative binding has been found, thus revealing the importance of the hydrophobic interactions in both kinds of surfactants. The Gibbs energies of binding per mole of surfactant (ΔGv̄) were calculated from the Wyman binding potential where, on the basis of the elevated number of binding sites, a statistical contribution has been included. Initially these energies are large and negative, gradually decreasing as saturation is approached. Changes in the slope of Gibbs energies have been identified with the saturation of the first binding set. These facts denote that the surfactants under study have different favorite adsorption sites along the protein and that the adsorption process of perfluorooctanoate is more closely followed by dodecanoate than by octanoate. Finally, electrophoresis and spectroscopy measurements suggest induced conformational changes on HSA depending on the surfactant mixture as well as the mixed ratio.
Fil: Blanco, Elena. Universidad de Santiago de Compostela. Facultad de Física; España
Fil: Messina, Paula Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Ruso, Juan M.. Universidad de Santiago de Compostela. Facultad de Física; España
Fil: Prieto, Gerardo. Universidad de Santiago de Compostela. Facultad de Física; España
Fil: Sarmiento, Félix. Universidad de Santiago de Compostela. Facultad de Física; España
Materia
HSA
HYDROCARBON SURFACTANTS
FLUORINATED SURFACTANTS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/95352
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Regarding the effect that different hydrocarbon/fluorocarbon surfactant mixtures have on their complexation with HSABlanco, ElenaMessina, Paula VerónicaRuso, Juan M.Prieto, GerardoSarmiento, FélixHSAHYDROCARBON SURFACTANTSFLUORINATED SURFACTANTShttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The complexations between human serum albumin (HSA) and the sodium perfluorooctanoate/sodium octanoate and sodium perfluorooctanoate/sodium dodecanoate systems have been studied by a combination of electrical conductivity, ion-selective electrode, electrophoresis, and spectroscopy measurements. The binary mixtures of the surfactants deviated slightly from ideality. Binding plots revealed the existence of two specific binding sites, the first site being more accessible than the second. Positive cooperative binding has been found, thus revealing the importance of the hydrophobic interactions in both kinds of surfactants. The Gibbs energies of binding per mole of surfactant (ΔGv̄) were calculated from the Wyman binding potential where, on the basis of the elevated number of binding sites, a statistical contribution has been included. Initially these energies are large and negative, gradually decreasing as saturation is approached. Changes in the slope of Gibbs energies have been identified with the saturation of the first binding set. These facts denote that the surfactants under study have different favorite adsorption sites along the protein and that the adsorption process of perfluorooctanoate is more closely followed by dodecanoate than by octanoate. Finally, electrophoresis and spectroscopy measurements suggest induced conformational changes on HSA depending on the surfactant mixture as well as the mixed ratio.Fil: Blanco, Elena. Universidad de Santiago de Compostela. Facultad de Física; EspañaFil: Messina, Paula Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Ruso, Juan M.. Universidad de Santiago de Compostela. Facultad de Física; EspañaFil: Prieto, Gerardo. Universidad de Santiago de Compostela. Facultad de Física; EspañaFil: Sarmiento, Félix. Universidad de Santiago de Compostela. Facultad de Física; EspañaAmerican Chemical Society2006-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/95352Blanco, Elena; Messina, Paula Verónica; Ruso, Juan M.; Prieto, Gerardo; Sarmiento, Félix; Regarding the effect that different hydrocarbon/fluorocarbon surfactant mixtures have on their complexation with HSA; American Chemical Society; Journal of Physical Chemistry B; 110; 23; 6-2006; 11369-113761520-6106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/jp060795hinfo:eu-repo/semantics/altIdentifier/doi/10.1021/jp060795hinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:06:25Zoai:ri.conicet.gov.ar:11336/95352instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:06:25.837CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Regarding the effect that different hydrocarbon/fluorocarbon surfactant mixtures have on their complexation with HSA
title Regarding the effect that different hydrocarbon/fluorocarbon surfactant mixtures have on their complexation with HSA
spellingShingle Regarding the effect that different hydrocarbon/fluorocarbon surfactant mixtures have on their complexation with HSA
Blanco, Elena
HSA
HYDROCARBON SURFACTANTS
FLUORINATED SURFACTANTS
title_short Regarding the effect that different hydrocarbon/fluorocarbon surfactant mixtures have on their complexation with HSA
title_full Regarding the effect that different hydrocarbon/fluorocarbon surfactant mixtures have on their complexation with HSA
title_fullStr Regarding the effect that different hydrocarbon/fluorocarbon surfactant mixtures have on their complexation with HSA
title_full_unstemmed Regarding the effect that different hydrocarbon/fluorocarbon surfactant mixtures have on their complexation with HSA
title_sort Regarding the effect that different hydrocarbon/fluorocarbon surfactant mixtures have on their complexation with HSA
dc.creator.none.fl_str_mv Blanco, Elena
Messina, Paula Verónica
Ruso, Juan M.
Prieto, Gerardo
Sarmiento, Félix
author Blanco, Elena
author_facet Blanco, Elena
Messina, Paula Verónica
Ruso, Juan M.
Prieto, Gerardo
Sarmiento, Félix
author_role author
author2 Messina, Paula Verónica
Ruso, Juan M.
Prieto, Gerardo
Sarmiento, Félix
author2_role author
author
author
author
dc.subject.none.fl_str_mv HSA
HYDROCARBON SURFACTANTS
FLUORINATED SURFACTANTS
topic HSA
HYDROCARBON SURFACTANTS
FLUORINATED SURFACTANTS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The complexations between human serum albumin (HSA) and the sodium perfluorooctanoate/sodium octanoate and sodium perfluorooctanoate/sodium dodecanoate systems have been studied by a combination of electrical conductivity, ion-selective electrode, electrophoresis, and spectroscopy measurements. The binary mixtures of the surfactants deviated slightly from ideality. Binding plots revealed the existence of two specific binding sites, the first site being more accessible than the second. Positive cooperative binding has been found, thus revealing the importance of the hydrophobic interactions in both kinds of surfactants. The Gibbs energies of binding per mole of surfactant (ΔGv̄) were calculated from the Wyman binding potential where, on the basis of the elevated number of binding sites, a statistical contribution has been included. Initially these energies are large and negative, gradually decreasing as saturation is approached. Changes in the slope of Gibbs energies have been identified with the saturation of the first binding set. These facts denote that the surfactants under study have different favorite adsorption sites along the protein and that the adsorption process of perfluorooctanoate is more closely followed by dodecanoate than by octanoate. Finally, electrophoresis and spectroscopy measurements suggest induced conformational changes on HSA depending on the surfactant mixture as well as the mixed ratio.
Fil: Blanco, Elena. Universidad de Santiago de Compostela. Facultad de Física; España
Fil: Messina, Paula Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Ruso, Juan M.. Universidad de Santiago de Compostela. Facultad de Física; España
Fil: Prieto, Gerardo. Universidad de Santiago de Compostela. Facultad de Física; España
Fil: Sarmiento, Félix. Universidad de Santiago de Compostela. Facultad de Física; España
description The complexations between human serum albumin (HSA) and the sodium perfluorooctanoate/sodium octanoate and sodium perfluorooctanoate/sodium dodecanoate systems have been studied by a combination of electrical conductivity, ion-selective electrode, electrophoresis, and spectroscopy measurements. The binary mixtures of the surfactants deviated slightly from ideality. Binding plots revealed the existence of two specific binding sites, the first site being more accessible than the second. Positive cooperative binding has been found, thus revealing the importance of the hydrophobic interactions in both kinds of surfactants. The Gibbs energies of binding per mole of surfactant (ΔGv̄) were calculated from the Wyman binding potential where, on the basis of the elevated number of binding sites, a statistical contribution has been included. Initially these energies are large and negative, gradually decreasing as saturation is approached. Changes in the slope of Gibbs energies have been identified with the saturation of the first binding set. These facts denote that the surfactants under study have different favorite adsorption sites along the protein and that the adsorption process of perfluorooctanoate is more closely followed by dodecanoate than by octanoate. Finally, electrophoresis and spectroscopy measurements suggest induced conformational changes on HSA depending on the surfactant mixture as well as the mixed ratio.
publishDate 2006
dc.date.none.fl_str_mv 2006-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/95352
Blanco, Elena; Messina, Paula Verónica; Ruso, Juan M.; Prieto, Gerardo; Sarmiento, Félix; Regarding the effect that different hydrocarbon/fluorocarbon surfactant mixtures have on their complexation with HSA; American Chemical Society; Journal of Physical Chemistry B; 110; 23; 6-2006; 11369-11376
1520-6106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/95352
identifier_str_mv Blanco, Elena; Messina, Paula Verónica; Ruso, Juan M.; Prieto, Gerardo; Sarmiento, Félix; Regarding the effect that different hydrocarbon/fluorocarbon surfactant mixtures have on their complexation with HSA; American Chemical Society; Journal of Physical Chemistry B; 110; 23; 6-2006; 11369-11376
1520-6106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/jp060795h
info:eu-repo/semantics/altIdentifier/doi/10.1021/jp060795h
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269958305742848
score 13.13397

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