Neospora caninum is a cause of perinatal mortality in axis deer (Axis axis)

Autores
Basso, Walter Ubaldo; Moré, Gastón Andrés; Quiroga, María Alejandra; Balducchi, Diego; Schares, Gereon; Venturini, María Cecilia
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Neospora caninum is a worldwide distributed protozoan that may cause neuromuscular disease in dogs and reproductive failure in domestic and wild ruminants. One axis fawn (Axis axis) and four neonates from the same deer herd died at a zoo in Argentina within a four-month period. The fawn presented with dilatation of the anal sphincter at birth and incontinence, developed weakness and ataxia and died at 14 days of age. At necropsy, a mega formation of the distal large intestine was observed. Microscopically, non-suppurative encephalitis, suppurative bronchopneumonia, fibrin necrotic enteritis and degenerative changes in the liver were observed in hematoxilin and eosin-stained tissue sections, and thick-walled N. caninum-like cysts were observed in fresh brain samples. Serologic studies for N. caninum revealed an IFAT titer of 1:6400 in the fawn and 1:25, 1:400, 1:3200 and 1:6400 in the neonates. N. caninum DNA was detected in brain samples from the fawn and from one neonate by PCR, and the parasite was isolated in vitro from the fawn´ brain after passage through gerbils (Meriones unguiculatus) and gamma-interferon knock-out mice. N. caninum DNA obtained from the fawn, neonate and isolated parasites showed the same microsatellite pattern. This suggests a common infection source for both animals. The diagnosis of N. caninum infection was confirmed, suggesting its association with perinatal mortality in captive axis deer. To the best of our knowledge, this is the first report of clinical disease associated to N. caninum infection in axis deer and of isolation of the parasite from this wild ruminant species.
Fil: Basso, Walter Ubaldo. Universitat Zurich; Suiza
Fil: Moré, Gastón Andrés. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Quiroga, María Alejandra. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Catedra de Patologia Especial; Argentina
Fil: Balducchi, Diego. Zoologico de La Plata; Argentina
Fil: Schares, Gereon. Institute of Epidemiology; Alemania
Fil: Venturini, María Cecilia. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Materia
Axis Axis;
Multilocus-Microsatellite Analysis
Neospora Caninum
Perinatal Mortality
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/31248
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/31248
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Neospora caninum is a cause of perinatal mortality in axis deer (Axis axis)Basso, Walter UbaldoMoré, Gastón AndrésQuiroga, María AlejandraBalducchi, DiegoSchares, GereonVenturini, María CeciliaAxis Axis;Multilocus-Microsatellite AnalysisNeospora CaninumPerinatal MortalityNeospora caninum is a worldwide distributed protozoan that may cause neuromuscular disease in dogs and reproductive failure in domestic and wild ruminants. One axis fawn (Axis axis) and four neonates from the same deer herd died at a zoo in Argentina within a four-month period. The fawn presented with dilatation of the anal sphincter at birth and incontinence, developed weakness and ataxia and died at 14 days of age. At necropsy, a mega formation of the distal large intestine was observed. Microscopically, non-suppurative encephalitis, suppurative bronchopneumonia, fibrin necrotic enteritis and degenerative changes in the liver were observed in hematoxilin and eosin-stained tissue sections, and thick-walled N. caninum-like cysts were observed in fresh brain samples. Serologic studies for N. caninum revealed an IFAT titer of 1:6400 in the fawn and 1:25, 1:400, 1:3200 and 1:6400 in the neonates. N. caninum DNA was detected in brain samples from the fawn and from one neonate by PCR, and the parasite was isolated in vitro from the fawn´ brain after passage through gerbils (Meriones unguiculatus) and gamma-interferon knock-out mice. N. caninum DNA obtained from the fawn, neonate and isolated parasites showed the same microsatellite pattern. This suggests a common infection source for both animals. The diagnosis of N. caninum infection was confirmed, suggesting its association with perinatal mortality in captive axis deer. To the best of our knowledge, this is the first report of clinical disease associated to N. caninum infection in axis deer and of isolation of the parasite from this wild ruminant species.Fil: Basso, Walter Ubaldo. Universitat Zurich; SuizaFil: Moré, Gastón Andrés. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Quiroga, María Alejandra. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Catedra de Patologia Especial; ArgentinaFil: Balducchi, Diego. Zoologico de La Plata; ArgentinaFil: Schares, Gereon. Institute of Epidemiology; AlemaniaFil: Venturini, María Cecilia. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; ArgentinaElsevier2014-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/31248Venturini, María Cecilia; Schares, Gereon; Balducchi, Diego; Quiroga, María Alejandra; Moré, Gastón Andrés; Basso, Walter Ubaldo; et al.; Neospora caninum is a cause of perinatal mortality in axis deer (Axis axis); Elsevier; Veterinary Parasitology; 199; 3-4; 1-2014; 255-2580304-4017CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.vetpar.2013.10.020info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0304401713005827info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:01:18Zoai:ri.conicet.gov.ar:11336/31248instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:01:18.753CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Neospora caninum is a cause of perinatal mortality in axis deer (Axis axis)
title Neospora caninum is a cause of perinatal mortality in axis deer (Axis axis)
spellingShingle Neospora caninum is a cause of perinatal mortality in axis deer (Axis axis)
Basso, Walter Ubaldo
Axis Axis;
Multilocus-Microsatellite Analysis
Neospora Caninum
Perinatal Mortality
title_short Neospora caninum is a cause of perinatal mortality in axis deer (Axis axis)
title_full Neospora caninum is a cause of perinatal mortality in axis deer (Axis axis)
title_fullStr Neospora caninum is a cause of perinatal mortality in axis deer (Axis axis)
title_full_unstemmed Neospora caninum is a cause of perinatal mortality in axis deer (Axis axis)
title_sort Neospora caninum is a cause of perinatal mortality in axis deer (Axis axis)
dc.creator.none.fl_str_mv Basso, Walter Ubaldo
Moré, Gastón Andrés
Quiroga, María Alejandra
Balducchi, Diego
Schares, Gereon
Venturini, María Cecilia
author Basso, Walter Ubaldo
author_facet Basso, Walter Ubaldo
Moré, Gastón Andrés
Quiroga, María Alejandra
Balducchi, Diego
Schares, Gereon
Venturini, María Cecilia
author_role author
author2 Moré, Gastón Andrés
Quiroga, María Alejandra
Balducchi, Diego
Schares, Gereon
Venturini, María Cecilia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Axis Axis;
Multilocus-Microsatellite Analysis
Neospora Caninum
Perinatal Mortality
topic Axis Axis;
Multilocus-Microsatellite Analysis
Neospora Caninum
Perinatal Mortality
dc.description.none.fl_txt_mv Neospora caninum is a worldwide distributed protozoan that may cause neuromuscular disease in dogs and reproductive failure in domestic and wild ruminants. One axis fawn (Axis axis) and four neonates from the same deer herd died at a zoo in Argentina within a four-month period. The fawn presented with dilatation of the anal sphincter at birth and incontinence, developed weakness and ataxia and died at 14 days of age. At necropsy, a mega formation of the distal large intestine was observed. Microscopically, non-suppurative encephalitis, suppurative bronchopneumonia, fibrin necrotic enteritis and degenerative changes in the liver were observed in hematoxilin and eosin-stained tissue sections, and thick-walled N. caninum-like cysts were observed in fresh brain samples. Serologic studies for N. caninum revealed an IFAT titer of 1:6400 in the fawn and 1:25, 1:400, 1:3200 and 1:6400 in the neonates. N. caninum DNA was detected in brain samples from the fawn and from one neonate by PCR, and the parasite was isolated in vitro from the fawn´ brain after passage through gerbils (Meriones unguiculatus) and gamma-interferon knock-out mice. N. caninum DNA obtained from the fawn, neonate and isolated parasites showed the same microsatellite pattern. This suggests a common infection source for both animals. The diagnosis of N. caninum infection was confirmed, suggesting its association with perinatal mortality in captive axis deer. To the best of our knowledge, this is the first report of clinical disease associated to N. caninum infection in axis deer and of isolation of the parasite from this wild ruminant species.
Fil: Basso, Walter Ubaldo. Universitat Zurich; Suiza
Fil: Moré, Gastón Andrés. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Quiroga, María Alejandra. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Catedra de Patologia Especial; Argentina
Fil: Balducchi, Diego. Zoologico de La Plata; Argentina
Fil: Schares, Gereon. Institute of Epidemiology; Alemania
Fil: Venturini, María Cecilia. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
description Neospora caninum is a worldwide distributed protozoan that may cause neuromuscular disease in dogs and reproductive failure in domestic and wild ruminants. One axis fawn (Axis axis) and four neonates from the same deer herd died at a zoo in Argentina within a four-month period. The fawn presented with dilatation of the anal sphincter at birth and incontinence, developed weakness and ataxia and died at 14 days of age. At necropsy, a mega formation of the distal large intestine was observed. Microscopically, non-suppurative encephalitis, suppurative bronchopneumonia, fibrin necrotic enteritis and degenerative changes in the liver were observed in hematoxilin and eosin-stained tissue sections, and thick-walled N. caninum-like cysts were observed in fresh brain samples. Serologic studies for N. caninum revealed an IFAT titer of 1:6400 in the fawn and 1:25, 1:400, 1:3200 and 1:6400 in the neonates. N. caninum DNA was detected in brain samples from the fawn and from one neonate by PCR, and the parasite was isolated in vitro from the fawn´ brain after passage through gerbils (Meriones unguiculatus) and gamma-interferon knock-out mice. N. caninum DNA obtained from the fawn, neonate and isolated parasites showed the same microsatellite pattern. This suggests a common infection source for both animals. The diagnosis of N. caninum infection was confirmed, suggesting its association with perinatal mortality in captive axis deer. To the best of our knowledge, this is the first report of clinical disease associated to N. caninum infection in axis deer and of isolation of the parasite from this wild ruminant species.
publishDate 2014
dc.date.none.fl_str_mv 2014-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/31248
Venturini, María Cecilia; Schares, Gereon; Balducchi, Diego; Quiroga, María Alejandra; Moré, Gastón Andrés; Basso, Walter Ubaldo; et al.; Neospora caninum is a cause of perinatal mortality in axis deer (Axis axis); Elsevier; Veterinary Parasitology; 199; 3-4; 1-2014; 255-258
0304-4017
CONICET Digital
CONICET
url http://hdl.handle.net/11336/31248
identifier_str_mv Venturini, María Cecilia; Schares, Gereon; Balducchi, Diego; Quiroga, María Alejandra; Moré, Gastón Andrés; Basso, Walter Ubaldo; et al.; Neospora caninum is a cause of perinatal mortality in axis deer (Axis axis); Elsevier; Veterinary Parasitology; 199; 3-4; 1-2014; 255-258
0304-4017
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vetpar.2013.10.020
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0304401713005827
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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