Isolation and molecular characterization of a new Neospora caninum isolate from cattle in Argentina

Autores
Campero, Lucía María; Venturini, María Cecilia; Moore, Dadin Prando; Massola, L.; Lagomarsino, H.; Garcia, B.; Bacigalupe, Diana; Rambeaud, Magdalena; Pardini, Lais Luján; Leunda, Maria Rosa; Schares, G; Campero, Carlos Manuel
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Neospora caninum is one of the most important causes of bovine abortion, but isolation of live parasites from infected tissue is difficult. The aims of the present study were to obtain new isolates of N. caninum from congenitally infected asymptomatic newborn cattle in Argentina and to perform characterization by multilocus-microsatellite analysis. Five clinically normal born calves, with demonstrable N. caninum antibodies in precolostrum serum by indirect fluorescent antibody test, were euthanized and their brain samples were processed for histopathological, immunohistochemical, polymerase chain reaction (PCR) analysis, and for bioassay in γ-interferon knockout (GKO) mice. Although N. caninum DNA was detected in brain from all the calves by PCR, viable N. caninum was isolated in GKO mice from only one calf. Neospora caninum tachyzoites of this Argentinean isolate, designated NC-Argentina LP1, were propagated in VERO cell cultures seeded with tachyzoites from the infected GKO mice tissues. Multilocus-microsatellite typing on DNA derived from cell cultured tachyzoites revealed a unique genetic pattern, different from reported isolates. This is the first bovine isolation and genetic characterization of N. caninum in Argentina.
Fil: Campero, Lucía María. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata; Argentina
Fil: Venturini, María Cecilia. Universidad Nacional de La Plata; Argentina
Fil: Moore, Dadin Prando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Massola, L.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Fil: Lagomarsino, H.. No especifica;
Fil: Garcia, B.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Fil: Bacigalupe, Diana. Universidad Nacional de La Plata; Argentina
Fil: Rambeaud, Magdalena. Universidad Nacional de La Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pardini, Lais Luján. Bunge And Born Foundation; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata; Argentina
Fil: Leunda, Maria Rosa. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Fil: Schares, G. Friedrich-Loeffler-Institute; Alemania
Fil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Materia
Argentina
Dairy Asymptomatic Calf
Isolate
Multilocus-Microsatellite Analysis
Neospora Caninum
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/75894
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/75894
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Isolation and molecular characterization of a new Neospora caninum isolate from cattle in ArgentinaCampero, Lucía MaríaVenturini, María CeciliaMoore, Dadin PrandoMassola, L.Lagomarsino, H.Garcia, B.Bacigalupe, DianaRambeaud, MagdalenaPardini, Lais LujánLeunda, Maria RosaSchares, GCampero, Carlos ManuelArgentinaDairy Asymptomatic CalfIsolateMultilocus-Microsatellite AnalysisNeospora Caninumhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Neospora caninum is one of the most important causes of bovine abortion, but isolation of live parasites from infected tissue is difficult. The aims of the present study were to obtain new isolates of N. caninum from congenitally infected asymptomatic newborn cattle in Argentina and to perform characterization by multilocus-microsatellite analysis. Five clinically normal born calves, with demonstrable N. caninum antibodies in precolostrum serum by indirect fluorescent antibody test, were euthanized and their brain samples were processed for histopathological, immunohistochemical, polymerase chain reaction (PCR) analysis, and for bioassay in γ-interferon knockout (GKO) mice. Although N. caninum DNA was detected in brain from all the calves by PCR, viable N. caninum was isolated in GKO mice from only one calf. Neospora caninum tachyzoites of this Argentinean isolate, designated NC-Argentina LP1, were propagated in VERO cell cultures seeded with tachyzoites from the infected GKO mice tissues. Multilocus-microsatellite typing on DNA derived from cell cultured tachyzoites revealed a unique genetic pattern, different from reported isolates. This is the first bovine isolation and genetic characterization of N. caninum in Argentina.Fil: Campero, Lucía María. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata; ArgentinaFil: Venturini, María Cecilia. Universidad Nacional de La Plata; ArgentinaFil: Moore, Dadin Prando. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Massola, L.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; ArgentinaFil: Lagomarsino, H.. No especifica;Fil: Garcia, B.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; ArgentinaFil: Bacigalupe, Diana. Universidad Nacional de La Plata; ArgentinaFil: Rambeaud, Magdalena. Universidad Nacional de La Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pardini, Lais Luján. Bunge And Born Foundation; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata; ArgentinaFil: Leunda, Maria Rosa. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; ArgentinaFil: Schares, G. Friedrich-Loeffler-Institute; AlemaniaFil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; ArgentinaAcademic Press Inc Elsevier Science2015-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/75894Campero, Lucía María; Venturini, María Cecilia; Moore, Dadin Prando; Massola, L.; Lagomarsino, H.; et al.; Isolation and molecular characterization of a new Neospora caninum isolate from cattle in Argentina; Academic Press Inc Elsevier Science; Experimental Parasitology; 155; 8-2015; 8-120014-4894CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.exppara.2015.04.009info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0014489415000983info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:12:43Zoai:ri.conicet.gov.ar:11336/75894instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:12:43.993CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Isolation and molecular characterization of a new Neospora caninum isolate from cattle in Argentina
title Isolation and molecular characterization of a new Neospora caninum isolate from cattle in Argentina
spellingShingle Isolation and molecular characterization of a new Neospora caninum isolate from cattle in Argentina
Campero, Lucía María
Argentina
Dairy Asymptomatic Calf
Isolate
Multilocus-Microsatellite Analysis
Neospora Caninum
title_short Isolation and molecular characterization of a new Neospora caninum isolate from cattle in Argentina
title_full Isolation and molecular characterization of a new Neospora caninum isolate from cattle in Argentina
title_fullStr Isolation and molecular characterization of a new Neospora caninum isolate from cattle in Argentina
title_full_unstemmed Isolation and molecular characterization of a new Neospora caninum isolate from cattle in Argentina
title_sort Isolation and molecular characterization of a new Neospora caninum isolate from cattle in Argentina
dc.creator.none.fl_str_mv Campero, Lucía María
Venturini, María Cecilia
Moore, Dadin Prando
Massola, L.
Lagomarsino, H.
Garcia, B.
Bacigalupe, Diana
Rambeaud, Magdalena
Pardini, Lais Luján
Leunda, Maria Rosa
Schares, G
Campero, Carlos Manuel
author Campero, Lucía María
author_facet Campero, Lucía María
Venturini, María Cecilia
Moore, Dadin Prando
Massola, L.
Lagomarsino, H.
Garcia, B.
Bacigalupe, Diana
Rambeaud, Magdalena
Pardini, Lais Luján
Leunda, Maria Rosa
Schares, G
Campero, Carlos Manuel
author_role author
author2 Venturini, María Cecilia
Moore, Dadin Prando
Massola, L.
Lagomarsino, H.
Garcia, B.
Bacigalupe, Diana
Rambeaud, Magdalena
Pardini, Lais Luján
Leunda, Maria Rosa
Schares, G
Campero, Carlos Manuel
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Argentina
Dairy Asymptomatic Calf
Isolate
Multilocus-Microsatellite Analysis
Neospora Caninum
topic Argentina
Dairy Asymptomatic Calf
Isolate
Multilocus-Microsatellite Analysis
Neospora Caninum
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Neospora caninum is one of the most important causes of bovine abortion, but isolation of live parasites from infected tissue is difficult. The aims of the present study were to obtain new isolates of N. caninum from congenitally infected asymptomatic newborn cattle in Argentina and to perform characterization by multilocus-microsatellite analysis. Five clinically normal born calves, with demonstrable N. caninum antibodies in precolostrum serum by indirect fluorescent antibody test, were euthanized and their brain samples were processed for histopathological, immunohistochemical, polymerase chain reaction (PCR) analysis, and for bioassay in γ-interferon knockout (GKO) mice. Although N. caninum DNA was detected in brain from all the calves by PCR, viable N. caninum was isolated in GKO mice from only one calf. Neospora caninum tachyzoites of this Argentinean isolate, designated NC-Argentina LP1, were propagated in VERO cell cultures seeded with tachyzoites from the infected GKO mice tissues. Multilocus-microsatellite typing on DNA derived from cell cultured tachyzoites revealed a unique genetic pattern, different from reported isolates. This is the first bovine isolation and genetic characterization of N. caninum in Argentina.
Fil: Campero, Lucía María. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata; Argentina
Fil: Venturini, María Cecilia. Universidad Nacional de La Plata; Argentina
Fil: Moore, Dadin Prando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Massola, L.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Fil: Lagomarsino, H.. No especifica;
Fil: Garcia, B.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Fil: Bacigalupe, Diana. Universidad Nacional de La Plata; Argentina
Fil: Rambeaud, Magdalena. Universidad Nacional de La Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pardini, Lais Luján. Bunge And Born Foundation; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata; Argentina
Fil: Leunda, Maria Rosa. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Fil: Schares, G. Friedrich-Loeffler-Institute; Alemania
Fil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
description Neospora caninum is one of the most important causes of bovine abortion, but isolation of live parasites from infected tissue is difficult. The aims of the present study were to obtain new isolates of N. caninum from congenitally infected asymptomatic newborn cattle in Argentina and to perform characterization by multilocus-microsatellite analysis. Five clinically normal born calves, with demonstrable N. caninum antibodies in precolostrum serum by indirect fluorescent antibody test, were euthanized and their brain samples were processed for histopathological, immunohistochemical, polymerase chain reaction (PCR) analysis, and for bioassay in γ-interferon knockout (GKO) mice. Although N. caninum DNA was detected in brain from all the calves by PCR, viable N. caninum was isolated in GKO mice from only one calf. Neospora caninum tachyzoites of this Argentinean isolate, designated NC-Argentina LP1, were propagated in VERO cell cultures seeded with tachyzoites from the infected GKO mice tissues. Multilocus-microsatellite typing on DNA derived from cell cultured tachyzoites revealed a unique genetic pattern, different from reported isolates. This is the first bovine isolation and genetic characterization of N. caninum in Argentina.
publishDate 2015
dc.date.none.fl_str_mv 2015-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/75894
Campero, Lucía María; Venturini, María Cecilia; Moore, Dadin Prando; Massola, L.; Lagomarsino, H.; et al.; Isolation and molecular characterization of a new Neospora caninum isolate from cattle in Argentina; Academic Press Inc Elsevier Science; Experimental Parasitology; 155; 8-2015; 8-12
0014-4894
CONICET Digital
CONICET
url http://hdl.handle.net/11336/75894
identifier_str_mv Campero, Lucía María; Venturini, María Cecilia; Moore, Dadin Prando; Massola, L.; Lagomarsino, H.; et al.; Isolation and molecular characterization of a new Neospora caninum isolate from cattle in Argentina; Academic Press Inc Elsevier Science; Experimental Parasitology; 155; 8-2015; 8-12
0014-4894
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.exppara.2015.04.009
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0014489415000983
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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