Response of UMR 106 cells exposed to titanium oxide and aluminum oxide nanoparticles

Autores
Di Virgilio, Ana Laura; Reigosa, Miguel Antonio; Fernandez Lorenzo, Monica Alicia
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The cytotoxicity potential of TiO2 and Al2O3 nanoparticles (NP) in UMR 106 cells was studied evaluating the lysosomal activity with neutral red uptake assay (NR), and the mitochondrial activity with tetrazolium MTT test. Different NP concentrations (10-300 microg/ml range) were used. A significant increase in the absorbance (stronger for TiO2 NP) was detected in both NR and MTT assays after 24 h exposure to the NP. However, the total cell proteins and the cell proliferation rate demonstrated that the cell viability decreased after 96 h exposure to NP. The formation of NP-containing vesicles within the cells was observed by TEM. Such event could explain the high cellular activity detected during the early stages of exposure, not related to the increase in cell viability. Results showed that the effects of NP on cell lines are dependent on the chemical composition of the particles, their concentration, exposure time and the type of treated cell. It can be concluded that the presence of TiO2 and Al2O3 NP in the cell surroundings can lead to cytotoxic effects. In the case of osteoblast cells, such events may induce osseointegration failures in orthopedic and dental implants that release NP.
Fil: Di Virgilio, Ana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Reigosa, Miguel Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina
Fil: Fernandez Lorenzo, Monica Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Materia
TITANIUM DIOXIDE
ALUMINA
CYTOTOXICITY
CELL CULTURE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/275354
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/275354
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Response of UMR 106 cells exposed to titanium oxide and aluminum oxide nanoparticlesDi Virgilio, Ana LauraReigosa, Miguel AntonioFernandez Lorenzo, Monica AliciaTITANIUM DIOXIDEALUMINACYTOTOXICITYCELL CULTUREhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2The cytotoxicity potential of TiO2 and Al2O3 nanoparticles (NP) in UMR 106 cells was studied evaluating the lysosomal activity with neutral red uptake assay (NR), and the mitochondrial activity with tetrazolium MTT test. Different NP concentrations (10-300 microg/ml range) were used. A significant increase in the absorbance (stronger for TiO2 NP) was detected in both NR and MTT assays after 24 h exposure to the NP. However, the total cell proteins and the cell proliferation rate demonstrated that the cell viability decreased after 96 h exposure to NP. The formation of NP-containing vesicles within the cells was observed by TEM. Such event could explain the high cellular activity detected during the early stages of exposure, not related to the increase in cell viability. Results showed that the effects of NP on cell lines are dependent on the chemical composition of the particles, their concentration, exposure time and the type of treated cell. It can be concluded that the presence of TiO2 and Al2O3 NP in the cell surroundings can lead to cytotoxic effects. In the case of osteoblast cells, such events may induce osseointegration failures in orthopedic and dental implants that release NP.Fil: Di Virgilio, Ana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Reigosa, Miguel Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; ArgentinaFil: Fernandez Lorenzo, Monica Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaWiley-liss, div John Wiley & Sons Inc.2009-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/275354Di Virgilio, Ana Laura; Reigosa, Miguel Antonio; Fernandez Lorenzo, Monica Alicia; Response of UMR 106 cells exposed to titanium oxide and aluminum oxide nanoparticles; Wiley-liss, div John Wiley & Sons Inc.; Journal of Biomedical Materials Research Part A; 92A; 1; 1-2009; 80-861549-3296CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1002/jbm.a.32339?utm_source=researchgate.net&medium=articleinfo:eu-repo/semantics/altIdentifier/doi/10.1002/jbm.a.32339info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-23T13:57:51Zoai:ri.conicet.gov.ar:11336/275354instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-23 13:57:51.453CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Response of UMR 106 cells exposed to titanium oxide and aluminum oxide nanoparticles
title Response of UMR 106 cells exposed to titanium oxide and aluminum oxide nanoparticles
spellingShingle Response of UMR 106 cells exposed to titanium oxide and aluminum oxide nanoparticles
Di Virgilio, Ana Laura
TITANIUM DIOXIDE
ALUMINA
CYTOTOXICITY
CELL CULTURE
title_short Response of UMR 106 cells exposed to titanium oxide and aluminum oxide nanoparticles
title_full Response of UMR 106 cells exposed to titanium oxide and aluminum oxide nanoparticles
title_fullStr Response of UMR 106 cells exposed to titanium oxide and aluminum oxide nanoparticles
title_full_unstemmed Response of UMR 106 cells exposed to titanium oxide and aluminum oxide nanoparticles
title_sort Response of UMR 106 cells exposed to titanium oxide and aluminum oxide nanoparticles
dc.creator.none.fl_str_mv Di Virgilio, Ana Laura
Reigosa, Miguel Antonio
Fernandez Lorenzo, Monica Alicia
author Di Virgilio, Ana Laura
author_facet Di Virgilio, Ana Laura
Reigosa, Miguel Antonio
Fernandez Lorenzo, Monica Alicia
author_role author
author2 Reigosa, Miguel Antonio
Fernandez Lorenzo, Monica Alicia
author2_role author
author
dc.subject.none.fl_str_mv TITANIUM DIOXIDE
ALUMINA
CYTOTOXICITY
CELL CULTURE
topic TITANIUM DIOXIDE
ALUMINA
CYTOTOXICITY
CELL CULTURE
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The cytotoxicity potential of TiO2 and Al2O3 nanoparticles (NP) in UMR 106 cells was studied evaluating the lysosomal activity with neutral red uptake assay (NR), and the mitochondrial activity with tetrazolium MTT test. Different NP concentrations (10-300 microg/ml range) were used. A significant increase in the absorbance (stronger for TiO2 NP) was detected in both NR and MTT assays after 24 h exposure to the NP. However, the total cell proteins and the cell proliferation rate demonstrated that the cell viability decreased after 96 h exposure to NP. The formation of NP-containing vesicles within the cells was observed by TEM. Such event could explain the high cellular activity detected during the early stages of exposure, not related to the increase in cell viability. Results showed that the effects of NP on cell lines are dependent on the chemical composition of the particles, their concentration, exposure time and the type of treated cell. It can be concluded that the presence of TiO2 and Al2O3 NP in the cell surroundings can lead to cytotoxic effects. In the case of osteoblast cells, such events may induce osseointegration failures in orthopedic and dental implants that release NP.
Fil: Di Virgilio, Ana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Reigosa, Miguel Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina
Fil: Fernandez Lorenzo, Monica Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
description The cytotoxicity potential of TiO2 and Al2O3 nanoparticles (NP) in UMR 106 cells was studied evaluating the lysosomal activity with neutral red uptake assay (NR), and the mitochondrial activity with tetrazolium MTT test. Different NP concentrations (10-300 microg/ml range) were used. A significant increase in the absorbance (stronger for TiO2 NP) was detected in both NR and MTT assays after 24 h exposure to the NP. However, the total cell proteins and the cell proliferation rate demonstrated that the cell viability decreased after 96 h exposure to NP. The formation of NP-containing vesicles within the cells was observed by TEM. Such event could explain the high cellular activity detected during the early stages of exposure, not related to the increase in cell viability. Results showed that the effects of NP on cell lines are dependent on the chemical composition of the particles, their concentration, exposure time and the type of treated cell. It can be concluded that the presence of TiO2 and Al2O3 NP in the cell surroundings can lead to cytotoxic effects. In the case of osteoblast cells, such events may induce osseointegration failures in orthopedic and dental implants that release NP.
publishDate 2009
dc.date.none.fl_str_mv 2009-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/275354
Di Virgilio, Ana Laura; Reigosa, Miguel Antonio; Fernandez Lorenzo, Monica Alicia; Response of UMR 106 cells exposed to titanium oxide and aluminum oxide nanoparticles; Wiley-liss, div John Wiley & Sons Inc.; Journal of Biomedical Materials Research Part A; 92A; 1; 1-2009; 80-86
1549-3296
CONICET Digital
CONICET
url http://hdl.handle.net/11336/275354
identifier_str_mv Di Virgilio, Ana Laura; Reigosa, Miguel Antonio; Fernandez Lorenzo, Monica Alicia; Response of UMR 106 cells exposed to titanium oxide and aluminum oxide nanoparticles; Wiley-liss, div John Wiley & Sons Inc.; Journal of Biomedical Materials Research Part A; 92A; 1; 1-2009; 80-86
1549-3296
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1002/jbm.a.32339?utm_source=researchgate.net&medium=article
info:eu-repo/semantics/altIdentifier/doi/10.1002/jbm.a.32339
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley-liss, div John Wiley & Sons Inc.
publisher.none.fl_str_mv Wiley-liss, div John Wiley & Sons Inc.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.952241

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