Effect of titanium dioxide on the oxidative metabolism of alveolar macrophages: An experimental study in rats

Autores
Olmedo, Daniel Gustavo; Tasat, Deborah Ruth; Guglielmotti, Maria Beatriz; Cabrini, Rómulo L.
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Metallic implants of titanium are used therapeutically in biomedicine because of its excellent biocompatibility. However, no metal or alloy is completely inert. We have previously shown that titanium oxide (TiO(2)) is transported in blood by phagocytic monocytes and deposited in organs such as liver, spleen, and lung 6 months after intraperitoneal injection (ip). Furthermore, it is well known that exposure to metal traces alters the cellular redox status. Thus, the aim of the present study was to determine the presence of titanium in target organs after chronic exposure, assess the potential structural alterations, and evaluate the oxidative metabolism of alveolar macrophages (AM) in the lung. Rats were ip injected with 1.60 g/100 g body wt of TiO(2) in saline solution. Organs (liver, spleen, lung) were processed for histological evaluation. Reactive oxygen species (ROS) in AM obtained by bronchoalveolar lavage (BAL) were evaluated using the nitroblue tetrazolium test and quantitative evaluation by digital image analysis. The histological analysis of organs revealed the presence of titanium in the parenchyma of these organs with no associated tissue damage. Although in lung alveolar macrophages TiO(2) induced a significant rise in ROS generation, it failed to cause tissue alteration. This finding may be attributed to an adaptive response.
Fil: Olmedo, Daniel Gustavo. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Tasat, Deborah Ruth. Universidad Nacional de San Martín; Argentina
Fil: Guglielmotti, Maria Beatriz. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Cabrini, Rómulo L.. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Anatomía Patológica; Argentina
Materia
Titanium dioxide
lungs
macrophages
oxidative metablism
rats
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/241809
Acceder
id CONICETDig_08879482283b1c0e26b0286321a32717
oai_identifier_str oai:ri.conicet.gov.ar:11336/241809
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of titanium dioxide on the oxidative metabolism of alveolar macrophages: An experimental study in ratsOlmedo, Daniel GustavoTasat, Deborah RuthGuglielmotti, Maria BeatrizCabrini, Rómulo L.Titanium dioxidelungsmacrophagesoxidative metablismratshttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Metallic implants of titanium are used therapeutically in biomedicine because of its excellent biocompatibility. However, no metal or alloy is completely inert. We have previously shown that titanium oxide (TiO(2)) is transported in blood by phagocytic monocytes and deposited in organs such as liver, spleen, and lung 6 months after intraperitoneal injection (ip). Furthermore, it is well known that exposure to metal traces alters the cellular redox status. Thus, the aim of the present study was to determine the presence of titanium in target organs after chronic exposure, assess the potential structural alterations, and evaluate the oxidative metabolism of alveolar macrophages (AM) in the lung. Rats were ip injected with 1.60 g/100 g body wt of TiO(2) in saline solution. Organs (liver, spleen, lung) were processed for histological evaluation. Reactive oxygen species (ROS) in AM obtained by bronchoalveolar lavage (BAL) were evaluated using the nitroblue tetrazolium test and quantitative evaluation by digital image analysis. The histological analysis of organs revealed the presence of titanium in the parenchyma of these organs with no associated tissue damage. Although in lung alveolar macrophages TiO(2) induced a significant rise in ROS generation, it failed to cause tissue alteration. This finding may be attributed to an adaptive response.Fil: Olmedo, Daniel Gustavo. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Tasat, Deborah Ruth. Universidad Nacional de San Martín; ArgentinaFil: Guglielmotti, Maria Beatriz. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Cabrini, Rómulo L.. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Anatomía Patológica; ArgentinaWiley-liss, div John Wiley & Sons Inc.2005-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/241809Olmedo, Daniel Gustavo; Tasat, Deborah Ruth; Guglielmotti, Maria Beatriz; Cabrini, Rómulo L.; Effect of titanium dioxide on the oxidative metabolism of alveolar macrophages: An experimental study in rats; Wiley-liss, div John Wiley & Sons Inc.; Journal of Biomedical Materials Research Part A; 73A; 2; 3-2005; 142-1491549-3296CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1002/jbm.a.30230info:eu-repo/semantics/altIdentifier/doi/10.1002/jbm.a.30230info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:44:00Zoai:ri.conicet.gov.ar:11336/241809instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:44:01.254CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of titanium dioxide on the oxidative metabolism of alveolar macrophages: An experimental study in rats
title Effect of titanium dioxide on the oxidative metabolism of alveolar macrophages: An experimental study in rats
spellingShingle Effect of titanium dioxide on the oxidative metabolism of alveolar macrophages: An experimental study in rats
Olmedo, Daniel Gustavo
Titanium dioxide
lungs
macrophages
oxidative metablism
rats
title_short Effect of titanium dioxide on the oxidative metabolism of alveolar macrophages: An experimental study in rats
title_full Effect of titanium dioxide on the oxidative metabolism of alveolar macrophages: An experimental study in rats
title_fullStr Effect of titanium dioxide on the oxidative metabolism of alveolar macrophages: An experimental study in rats
title_full_unstemmed Effect of titanium dioxide on the oxidative metabolism of alveolar macrophages: An experimental study in rats
title_sort Effect of titanium dioxide on the oxidative metabolism of alveolar macrophages: An experimental study in rats
dc.creator.none.fl_str_mv Olmedo, Daniel Gustavo
Tasat, Deborah Ruth
Guglielmotti, Maria Beatriz
Cabrini, Rómulo L.
author Olmedo, Daniel Gustavo
author_facet Olmedo, Daniel Gustavo
Tasat, Deborah Ruth
Guglielmotti, Maria Beatriz
Cabrini, Rómulo L.
author_role author
author2 Tasat, Deborah Ruth
Guglielmotti, Maria Beatriz
Cabrini, Rómulo L.
author2_role author
author
author
dc.subject.none.fl_str_mv Titanium dioxide
lungs
macrophages
oxidative metablism
rats
topic Titanium dioxide
lungs
macrophages
oxidative metablism
rats
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Metallic implants of titanium are used therapeutically in biomedicine because of its excellent biocompatibility. However, no metal or alloy is completely inert. We have previously shown that titanium oxide (TiO(2)) is transported in blood by phagocytic monocytes and deposited in organs such as liver, spleen, and lung 6 months after intraperitoneal injection (ip). Furthermore, it is well known that exposure to metal traces alters the cellular redox status. Thus, the aim of the present study was to determine the presence of titanium in target organs after chronic exposure, assess the potential structural alterations, and evaluate the oxidative metabolism of alveolar macrophages (AM) in the lung. Rats were ip injected with 1.60 g/100 g body wt of TiO(2) in saline solution. Organs (liver, spleen, lung) were processed for histological evaluation. Reactive oxygen species (ROS) in AM obtained by bronchoalveolar lavage (BAL) were evaluated using the nitroblue tetrazolium test and quantitative evaluation by digital image analysis. The histological analysis of organs revealed the presence of titanium in the parenchyma of these organs with no associated tissue damage. Although in lung alveolar macrophages TiO(2) induced a significant rise in ROS generation, it failed to cause tissue alteration. This finding may be attributed to an adaptive response.
Fil: Olmedo, Daniel Gustavo. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Tasat, Deborah Ruth. Universidad Nacional de San Martín; Argentina
Fil: Guglielmotti, Maria Beatriz. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Cabrini, Rómulo L.. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Anatomía Patológica; Argentina
description Metallic implants of titanium are used therapeutically in biomedicine because of its excellent biocompatibility. However, no metal or alloy is completely inert. We have previously shown that titanium oxide (TiO(2)) is transported in blood by phagocytic monocytes and deposited in organs such as liver, spleen, and lung 6 months after intraperitoneal injection (ip). Furthermore, it is well known that exposure to metal traces alters the cellular redox status. Thus, the aim of the present study was to determine the presence of titanium in target organs after chronic exposure, assess the potential structural alterations, and evaluate the oxidative metabolism of alveolar macrophages (AM) in the lung. Rats were ip injected with 1.60 g/100 g body wt of TiO(2) in saline solution. Organs (liver, spleen, lung) were processed for histological evaluation. Reactive oxygen species (ROS) in AM obtained by bronchoalveolar lavage (BAL) were evaluated using the nitroblue tetrazolium test and quantitative evaluation by digital image analysis. The histological analysis of organs revealed the presence of titanium in the parenchyma of these organs with no associated tissue damage. Although in lung alveolar macrophages TiO(2) induced a significant rise in ROS generation, it failed to cause tissue alteration. This finding may be attributed to an adaptive response.
publishDate 2005
dc.date.none.fl_str_mv 2005-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/241809
Olmedo, Daniel Gustavo; Tasat, Deborah Ruth; Guglielmotti, Maria Beatriz; Cabrini, Rómulo L.; Effect of titanium dioxide on the oxidative metabolism of alveolar macrophages: An experimental study in rats; Wiley-liss, div John Wiley & Sons Inc.; Journal of Biomedical Materials Research Part A; 73A; 2; 3-2005; 142-149
1549-3296
CONICET Digital
CONICET
url http://hdl.handle.net/11336/241809
identifier_str_mv Olmedo, Daniel Gustavo; Tasat, Deborah Ruth; Guglielmotti, Maria Beatriz; Cabrini, Rómulo L.; Effect of titanium dioxide on the oxidative metabolism of alveolar macrophages: An experimental study in rats; Wiley-liss, div John Wiley & Sons Inc.; Journal of Biomedical Materials Research Part A; 73A; 2; 3-2005; 142-149
1549-3296
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1002/jbm.a.30230
info:eu-repo/semantics/altIdentifier/doi/10.1002/jbm.a.30230
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley-liss, div John Wiley & Sons Inc.
publisher.none.fl_str_mv Wiley-liss, div John Wiley & Sons Inc.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268639210766336
score 13.13397

Publicaciones similares