In vitro age dependent response of macrophages to micro and nano titanium dioxide particles

Autores
Bruno, Marcos E.; Sittner, Maximiliano; Cabrini, Romulo L.; Guglielmotti, Maria Beatriz; Olmedo, Daniel Gustavo; Tasat, Deborah R.
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
As a result of corrosion, microparticles (MP) and/or nanoparticles (NP) can be released from the metallic implants surface into the bioenvironment. The biological response to these particles depends not only on the physico-chemical properties of the particles but also on host factors, such as age. Macrophages have attracted wide concern in biomedicine. The aim of this investigation was to study the age related biological response of macrophages to TiO2-MP and NP in vitro. Alveolar macrophages (AM) obtained from young and senescent rats were cultured and exposed to TiO2-MP and NP. Cell metabolism, superoxide anion (O2−) and nitric oxide (NO) generation, and cytokine release (IL-6, TNFα, IL-10) were measured. Cell metabolism was not affected by particle exposure. O2− and NO generation increased in a dose dependent manner. A marked increase on IL-6 release was found in the young-AM subpopulation exposed to TiO2-MP. Conversely, both particle sizes induced a dose dependent release of TNFα in senescent-AM. Only the highest concentration of TiO2-particles caused a significant increase in IL-10 release in AM-cultures. These observations lend strong support to the suggestion that cellular response of macrophages to TiO2-particles is age dependent. The biological effect of the particles would seem to be more deleterious in the senescent age-group.
Fil: Bruno, Marcos E.. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina
Fil: Sittner, Maximiliano. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina
Fil: Cabrini, Romulo L.. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina. Comisión Nacional de Energía Atómica. Gerencia de Area de Aplicaciones de la Tecnología Nuclear. Departamento de Radiobiología; Argentina
Fil: Guglielmotti, Maria Beatriz. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Olmedo, Daniel Gustavo. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tasat, Deborah R.. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Histología y Embriología; Argentina
Materia
Macropahges
Titanium Dioxide
Aging
Inflammatory Mediators
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/16781
Acceder
id CONICETDig_65505b8a705f02f9b9bf1f24dc45993f
oai_identifier_str oai:ri.conicet.gov.ar:11336/16781
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling In vitro age dependent response of macrophages to micro and nano titanium dioxide particlesBruno, Marcos E.Sittner, MaximilianoCabrini, Romulo L.Guglielmotti, Maria BeatrizOlmedo, Daniel GustavoTasat, Deborah R.MacropahgesTitanium DioxideAgingInflammatory Mediatorshttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3As a result of corrosion, microparticles (MP) and/or nanoparticles (NP) can be released from the metallic implants surface into the bioenvironment. The biological response to these particles depends not only on the physico-chemical properties of the particles but also on host factors, such as age. Macrophages have attracted wide concern in biomedicine. The aim of this investigation was to study the age related biological response of macrophages to TiO2-MP and NP in vitro. Alveolar macrophages (AM) obtained from young and senescent rats were cultured and exposed to TiO2-MP and NP. Cell metabolism, superoxide anion (O2−) and nitric oxide (NO) generation, and cytokine release (IL-6, TNFα, IL-10) were measured. Cell metabolism was not affected by particle exposure. O2− and NO generation increased in a dose dependent manner. A marked increase on IL-6 release was found in the young-AM subpopulation exposed to TiO2-MP. Conversely, both particle sizes induced a dose dependent release of TNFα in senescent-AM. Only the highest concentration of TiO2-particles caused a significant increase in IL-10 release in AM-cultures. These observations lend strong support to the suggestion that cellular response of macrophages to TiO2-particles is age dependent. The biological effect of the particles would seem to be more deleterious in the senescent age-group.Fil: Bruno, Marcos E.. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; ArgentinaFil: Sittner, Maximiliano. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; ArgentinaFil: Cabrini, Romulo L.. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina. Comisión Nacional de Energía Atómica. Gerencia de Area de Aplicaciones de la Tecnología Nuclear. Departamento de Radiobiología; ArgentinaFil: Guglielmotti, Maria Beatriz. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Olmedo, Daniel Gustavo. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Tasat, Deborah R.. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Histología y Embriología; ArgentinaWiley2015-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/16781Bruno, Marcos E.; Sittner, Maximiliano; Cabrini, Romulo L.; Guglielmotti, Maria Beatriz; Olmedo, Daniel Gustavo; et al.; In vitro age dependent response of macrophages to micro and nano titanium dioxide particles; Wiley; Journal Of Biomedical Materials Research Part A; 103; 2; 2-2015; 471-4781549-32961552-4965enginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/jbm.a.35194/abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1002/jbm.a.35194info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:00:12Zoai:ri.conicet.gov.ar:11336/16781instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:00:13.19CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In vitro age dependent response of macrophages to micro and nano titanium dioxide particles
title In vitro age dependent response of macrophages to micro and nano titanium dioxide particles
spellingShingle In vitro age dependent response of macrophages to micro and nano titanium dioxide particles
Bruno, Marcos E.
Macropahges
Titanium Dioxide
Aging
Inflammatory Mediators
title_short In vitro age dependent response of macrophages to micro and nano titanium dioxide particles
title_full In vitro age dependent response of macrophages to micro and nano titanium dioxide particles
title_fullStr In vitro age dependent response of macrophages to micro and nano titanium dioxide particles
title_full_unstemmed In vitro age dependent response of macrophages to micro and nano titanium dioxide particles
title_sort In vitro age dependent response of macrophages to micro and nano titanium dioxide particles
dc.creator.none.fl_str_mv Bruno, Marcos E.
Sittner, Maximiliano
Cabrini, Romulo L.
Guglielmotti, Maria Beatriz
Olmedo, Daniel Gustavo
Tasat, Deborah R.
author Bruno, Marcos E.
author_facet Bruno, Marcos E.
Sittner, Maximiliano
Cabrini, Romulo L.
Guglielmotti, Maria Beatriz
Olmedo, Daniel Gustavo
Tasat, Deborah R.
author_role author
author2 Sittner, Maximiliano
Cabrini, Romulo L.
Guglielmotti, Maria Beatriz
Olmedo, Daniel Gustavo
Tasat, Deborah R.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Macropahges
Titanium Dioxide
Aging
Inflammatory Mediators
topic Macropahges
Titanium Dioxide
Aging
Inflammatory Mediators
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv As a result of corrosion, microparticles (MP) and/or nanoparticles (NP) can be released from the metallic implants surface into the bioenvironment. The biological response to these particles depends not only on the physico-chemical properties of the particles but also on host factors, such as age. Macrophages have attracted wide concern in biomedicine. The aim of this investigation was to study the age related biological response of macrophages to TiO2-MP and NP in vitro. Alveolar macrophages (AM) obtained from young and senescent rats were cultured and exposed to TiO2-MP and NP. Cell metabolism, superoxide anion (O2−) and nitric oxide (NO) generation, and cytokine release (IL-6, TNFα, IL-10) were measured. Cell metabolism was not affected by particle exposure. O2− and NO generation increased in a dose dependent manner. A marked increase on IL-6 release was found in the young-AM subpopulation exposed to TiO2-MP. Conversely, both particle sizes induced a dose dependent release of TNFα in senescent-AM. Only the highest concentration of TiO2-particles caused a significant increase in IL-10 release in AM-cultures. These observations lend strong support to the suggestion that cellular response of macrophages to TiO2-particles is age dependent. The biological effect of the particles would seem to be more deleterious in the senescent age-group.
Fil: Bruno, Marcos E.. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina
Fil: Sittner, Maximiliano. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina
Fil: Cabrini, Romulo L.. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina. Comisión Nacional de Energía Atómica. Gerencia de Area de Aplicaciones de la Tecnología Nuclear. Departamento de Radiobiología; Argentina
Fil: Guglielmotti, Maria Beatriz. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Olmedo, Daniel Gustavo. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tasat, Deborah R.. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Histología y Embriología; Argentina
description As a result of corrosion, microparticles (MP) and/or nanoparticles (NP) can be released from the metallic implants surface into the bioenvironment. The biological response to these particles depends not only on the physico-chemical properties of the particles but also on host factors, such as age. Macrophages have attracted wide concern in biomedicine. The aim of this investigation was to study the age related biological response of macrophages to TiO2-MP and NP in vitro. Alveolar macrophages (AM) obtained from young and senescent rats were cultured and exposed to TiO2-MP and NP. Cell metabolism, superoxide anion (O2−) and nitric oxide (NO) generation, and cytokine release (IL-6, TNFα, IL-10) were measured. Cell metabolism was not affected by particle exposure. O2− and NO generation increased in a dose dependent manner. A marked increase on IL-6 release was found in the young-AM subpopulation exposed to TiO2-MP. Conversely, both particle sizes induced a dose dependent release of TNFα in senescent-AM. Only the highest concentration of TiO2-particles caused a significant increase in IL-10 release in AM-cultures. These observations lend strong support to the suggestion that cellular response of macrophages to TiO2-particles is age dependent. The biological effect of the particles would seem to be more deleterious in the senescent age-group.
publishDate 2015
dc.date.none.fl_str_mv 2015-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/16781
Bruno, Marcos E.; Sittner, Maximiliano; Cabrini, Romulo L.; Guglielmotti, Maria Beatriz; Olmedo, Daniel Gustavo; et al.; In vitro age dependent response of macrophages to micro and nano titanium dioxide particles; Wiley; Journal Of Biomedical Materials Research Part A; 103; 2; 2-2015; 471-478
1549-3296
1552-4965
url http://hdl.handle.net/11336/16781
identifier_str_mv Bruno, Marcos E.; Sittner, Maximiliano; Cabrini, Romulo L.; Guglielmotti, Maria Beatriz; Olmedo, Daniel Gustavo; et al.; In vitro age dependent response of macrophages to micro and nano titanium dioxide particles; Wiley; Journal Of Biomedical Materials Research Part A; 103; 2; 2-2015; 471-478
1549-3296
1552-4965
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/jbm.a.35194/abstract
info:eu-repo/semantics/altIdentifier/doi/10.1002/jbm.a.35194
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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