In vitro and in vivo digestibility of prebiotic galactooligosacharides synthesized by β ‐galactosidase from Lactobacillus delbruecki subsp. bulgaricus CRL450

Autores
Fara, María Agustina; Hernández Hernández, Oswaldo; Palacios, Jorge Martin; Montilla, Antonia; Zarate, Gabriela del Valle
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
BACKGROUND: To date, the general belief that prebiotics reach the colon without any alterations is challenged. Some in vitro and in vivo studies have demonstrated that "non-digestible" oligosaccharides are digested to different degrees depending on their structural composition. In this work, we compared different methods to assess the digestibility of oligosaccharides synthesized by β-galactosidase (β-gal) of Lactobacillus delbruecki subsp. bulgaricus CRL450 (CRL450-β-gal) from lactose, lactulose and lactitol. RESULTS: In simulated gastrointestinal fluid (SGIF) method no changes were observed. However, the oligosaccharides synthesized by CRL450-β-gal were partially hydrolyzed in vitro, depending on their structure and composition, with rat small intestinal extract (RSIE) and small intestinal brush-border membrane vesicles (BBMV) from pig. Digestion of some oligosaccharides increased when mixtures were fed to C57BL/6 mice used as in vivo model, however, lactulose-oligosaccharides were the most resistant to the physiological conditions of mice. In general β(1→6) linked products showed higher resistance compared to β(1→3) oligosaccharides. CONCLUSION: In vitro digestion methods, without disaccharidases, may underestimate the importance of carbohydrates hydrolysis in the small intestine. Although BVMM and RSIE digestion assays are appropriate in vitro methods for these studies, in vivo studies remain the most reliable for understanding what actually happens in the digestion of oligosaccharides.
Fil: Fara, María Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Hernández Hernández, Oswaldo. Consejo Superior de Investigaciones Científicas. Instituto de Investigación en Ciencias de la Alimentación; España
Fil: Palacios, Jorge Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Montilla, Antonia. Consejo Superior de Investigaciones Científicas. Instituto de Investigación en Ciencias de la Alimentación; España
Fil: Zarate, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad San Pablo Tucumán; Argentina
Materia
LACTOBACILLUS DELBRUECKI SUBSP BULGARICUS
OLIGOSACCHARIDES
DIGESTION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/261907
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/261907
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling In vitro and in vivo digestibility of prebiotic galactooligosacharides synthesized by β ‐galactosidase from Lactobacillus delbruecki subsp. bulgaricus CRL450Fara, María AgustinaHernández Hernández, OswaldoPalacios, Jorge MartinMontilla, AntoniaZarate, Gabriela del ValleLACTOBACILLUS DELBRUECKI SUBSP BULGARICUSOLIGOSACCHARIDESDIGESTIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1BACKGROUND: To date, the general belief that prebiotics reach the colon without any alterations is challenged. Some in vitro and in vivo studies have demonstrated that "non-digestible" oligosaccharides are digested to different degrees depending on their structural composition. In this work, we compared different methods to assess the digestibility of oligosaccharides synthesized by β-galactosidase (β-gal) of Lactobacillus delbruecki subsp. bulgaricus CRL450 (CRL450-β-gal) from lactose, lactulose and lactitol. RESULTS: In simulated gastrointestinal fluid (SGIF) method no changes were observed. However, the oligosaccharides synthesized by CRL450-β-gal were partially hydrolyzed in vitro, depending on their structure and composition, with rat small intestinal extract (RSIE) and small intestinal brush-border membrane vesicles (BBMV) from pig. Digestion of some oligosaccharides increased when mixtures were fed to C57BL/6 mice used as in vivo model, however, lactulose-oligosaccharides were the most resistant to the physiological conditions of mice. In general β(1→6) linked products showed higher resistance compared to β(1→3) oligosaccharides. CONCLUSION: In vitro digestion methods, without disaccharidases, may underestimate the importance of carbohydrates hydrolysis in the small intestine. Although BVMM and RSIE digestion assays are appropriate in vitro methods for these studies, in vivo studies remain the most reliable for understanding what actually happens in the digestion of oligosaccharides.Fil: Fara, María Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Hernández Hernández, Oswaldo. Consejo Superior de Investigaciones Científicas. Instituto de Investigación en Ciencias de la Alimentación; EspañaFil: Palacios, Jorge Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Montilla, Antonia. Consejo Superior de Investigaciones Científicas. Instituto de Investigación en Ciencias de la Alimentación; EspañaFil: Zarate, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad San Pablo Tucumán; ArgentinaJohn Wiley & Sons Ltd2024-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/261907Fara, María Agustina; Hernández Hernández, Oswaldo; Palacios, Jorge Martin; Montilla, Antonia; Zarate, Gabriela del Valle; In vitro and in vivo digestibility of prebiotic galactooligosacharides synthesized by β ‐galactosidase from Lactobacillus delbruecki subsp. bulgaricus CRL450; John Wiley & Sons Ltd; Journal of the Science of Food and Agriculture; 104; 11; 4-2024; 6769-67770022-5142CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://scijournals.onlinelibrary.wiley.com/doi/full/10.1002/jsfa.13504info:eu-repo/semantics/altIdentifier/doi/10.1002/jsfa.13504info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:09:58Zoai:ri.conicet.gov.ar:11336/261907instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:09:58.614CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In vitro and in vivo digestibility of prebiotic galactooligosacharides synthesized by β ‐galactosidase from Lactobacillus delbruecki subsp. bulgaricus CRL450
title In vitro and in vivo digestibility of prebiotic galactooligosacharides synthesized by β ‐galactosidase from Lactobacillus delbruecki subsp. bulgaricus CRL450
spellingShingle In vitro and in vivo digestibility of prebiotic galactooligosacharides synthesized by β ‐galactosidase from Lactobacillus delbruecki subsp. bulgaricus CRL450
Fara, María Agustina
LACTOBACILLUS DELBRUECKI SUBSP BULGARICUS
OLIGOSACCHARIDES
DIGESTION
title_short In vitro and in vivo digestibility of prebiotic galactooligosacharides synthesized by β ‐galactosidase from Lactobacillus delbruecki subsp. bulgaricus CRL450
title_full In vitro and in vivo digestibility of prebiotic galactooligosacharides synthesized by β ‐galactosidase from Lactobacillus delbruecki subsp. bulgaricus CRL450
title_fullStr In vitro and in vivo digestibility of prebiotic galactooligosacharides synthesized by β ‐galactosidase from Lactobacillus delbruecki subsp. bulgaricus CRL450
title_full_unstemmed In vitro and in vivo digestibility of prebiotic galactooligosacharides synthesized by β ‐galactosidase from Lactobacillus delbruecki subsp. bulgaricus CRL450
title_sort In vitro and in vivo digestibility of prebiotic galactooligosacharides synthesized by β ‐galactosidase from Lactobacillus delbruecki subsp. bulgaricus CRL450
dc.creator.none.fl_str_mv Fara, María Agustina
Hernández Hernández, Oswaldo
Palacios, Jorge Martin
Montilla, Antonia
Zarate, Gabriela del Valle
author Fara, María Agustina
author_facet Fara, María Agustina
Hernández Hernández, Oswaldo
Palacios, Jorge Martin
Montilla, Antonia
Zarate, Gabriela del Valle
author_role author
author2 Hernández Hernández, Oswaldo
Palacios, Jorge Martin
Montilla, Antonia
Zarate, Gabriela del Valle
author2_role author
author
author
author
dc.subject.none.fl_str_mv LACTOBACILLUS DELBRUECKI SUBSP BULGARICUS
OLIGOSACCHARIDES
DIGESTION
topic LACTOBACILLUS DELBRUECKI SUBSP BULGARICUS
OLIGOSACCHARIDES
DIGESTION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv BACKGROUND: To date, the general belief that prebiotics reach the colon without any alterations is challenged. Some in vitro and in vivo studies have demonstrated that "non-digestible" oligosaccharides are digested to different degrees depending on their structural composition. In this work, we compared different methods to assess the digestibility of oligosaccharides synthesized by β-galactosidase (β-gal) of Lactobacillus delbruecki subsp. bulgaricus CRL450 (CRL450-β-gal) from lactose, lactulose and lactitol. RESULTS: In simulated gastrointestinal fluid (SGIF) method no changes were observed. However, the oligosaccharides synthesized by CRL450-β-gal were partially hydrolyzed in vitro, depending on their structure and composition, with rat small intestinal extract (RSIE) and small intestinal brush-border membrane vesicles (BBMV) from pig. Digestion of some oligosaccharides increased when mixtures were fed to C57BL/6 mice used as in vivo model, however, lactulose-oligosaccharides were the most resistant to the physiological conditions of mice. In general β(1→6) linked products showed higher resistance compared to β(1→3) oligosaccharides. CONCLUSION: In vitro digestion methods, without disaccharidases, may underestimate the importance of carbohydrates hydrolysis in the small intestine. Although BVMM and RSIE digestion assays are appropriate in vitro methods for these studies, in vivo studies remain the most reliable for understanding what actually happens in the digestion of oligosaccharides.
Fil: Fara, María Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Hernández Hernández, Oswaldo. Consejo Superior de Investigaciones Científicas. Instituto de Investigación en Ciencias de la Alimentación; España
Fil: Palacios, Jorge Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Montilla, Antonia. Consejo Superior de Investigaciones Científicas. Instituto de Investigación en Ciencias de la Alimentación; España
Fil: Zarate, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad San Pablo Tucumán; Argentina
description BACKGROUND: To date, the general belief that prebiotics reach the colon without any alterations is challenged. Some in vitro and in vivo studies have demonstrated that "non-digestible" oligosaccharides are digested to different degrees depending on their structural composition. In this work, we compared different methods to assess the digestibility of oligosaccharides synthesized by β-galactosidase (β-gal) of Lactobacillus delbruecki subsp. bulgaricus CRL450 (CRL450-β-gal) from lactose, lactulose and lactitol. RESULTS: In simulated gastrointestinal fluid (SGIF) method no changes were observed. However, the oligosaccharides synthesized by CRL450-β-gal were partially hydrolyzed in vitro, depending on their structure and composition, with rat small intestinal extract (RSIE) and small intestinal brush-border membrane vesicles (BBMV) from pig. Digestion of some oligosaccharides increased when mixtures were fed to C57BL/6 mice used as in vivo model, however, lactulose-oligosaccharides were the most resistant to the physiological conditions of mice. In general β(1→6) linked products showed higher resistance compared to β(1→3) oligosaccharides. CONCLUSION: In vitro digestion methods, without disaccharidases, may underestimate the importance of carbohydrates hydrolysis in the small intestine. Although BVMM and RSIE digestion assays are appropriate in vitro methods for these studies, in vivo studies remain the most reliable for understanding what actually happens in the digestion of oligosaccharides.
publishDate 2024
dc.date.none.fl_str_mv 2024-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/261907
Fara, María Agustina; Hernández Hernández, Oswaldo; Palacios, Jorge Martin; Montilla, Antonia; Zarate, Gabriela del Valle; In vitro and in vivo digestibility of prebiotic galactooligosacharides synthesized by β ‐galactosidase from Lactobacillus delbruecki subsp. bulgaricus CRL450; John Wiley & Sons Ltd; Journal of the Science of Food and Agriculture; 104; 11; 4-2024; 6769-6777
0022-5142
CONICET Digital
CONICET
url http://hdl.handle.net/11336/261907
identifier_str_mv Fara, María Agustina; Hernández Hernández, Oswaldo; Palacios, Jorge Martin; Montilla, Antonia; Zarate, Gabriela del Valle; In vitro and in vivo digestibility of prebiotic galactooligosacharides synthesized by β ‐galactosidase from Lactobacillus delbruecki subsp. bulgaricus CRL450; John Wiley & Sons Ltd; Journal of the Science of Food and Agriculture; 104; 11; 4-2024; 6769-6777
0022-5142
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://scijournals.onlinelibrary.wiley.com/doi/full/10.1002/jsfa.13504
info:eu-repo/semantics/altIdentifier/doi/10.1002/jsfa.13504
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons Ltd
publisher.none.fl_str_mv John Wiley & Sons Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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