Prebiotic galactooligosaccharides production from lactose and lactulose by Lactobacillus delbrueckii subsp. bulgaricus CRL450

Autores
Fara, María Agustina; Sabater, Carlos Edgardo Alejandro; Palacios, Jorge Martin; Requena, Teresa; Montilla Corredera, Antonia; Zárate, Gabriela
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Galactooligosaccharides (GOS) are useful dietary ingredients recognized worldwide as prebiotics. In the present study, we evaluated the β-galactosidase (β-gal) activity of a panel of lactic acid bacteria (LAB) in order to select strains for the synthesis of oligosaccharides from lactose (GOS) and lactulose (OsLu) with a potential prebiotic effect. Fifteen strains out of 20 were able to grow on lactose and showed β-gal activities between 0.03 and 2.06 U mg−1, whereas eleven were able to synthesize GOS. Lactobacillus delbrueckii subsp. bulgaricus CRL450, the strain with the highest β-gal activity, synthesized a maximum of 41.3% GOS and 21.0% OsLu from lactose and lactulose, respectively, with β-(1 → 6) and secondary β-(1 → 3) linkages. When these compounds were tested without purifying, as carbon sources for the development of recognized probiotics and the producer strain, high growth was observed compared to non-prebiotic sugars like glucose and lactose. When the purified oligosaccharides were tested, the bacterial growth decreased, but the microorganisms displayed metabolic activity evidenced by the consumption of carbohydrates and the production of lactic acid. Additionally, the purified oligosaccharides demonstrated a bifidogenic effect. The obtained results support the potential of L. delbrueckii subsp. bulgaricus CRL450 for the production of the prebiotics GOS and OsLu and encourage the optimization of their synthesis for the design of new functional food ingredients.
Fil: Fara, María Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Sabater, Carlos Edgardo Alejandro. Consejo Superior de Investigaciones Científicas. Instituto de Investigación en Ciencias de la Alimentación; España. Universidad Autónoma de Madrid; España
Fil: Palacios, Jorge Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Requena, Teresa. Consejo Superior de Investigaciones Científicas. Instituto de Investigación en Ciencias de la Alimentación; España. Universidad Autónoma de Madrid; España
Fil: Montilla Corredera, Antonia. Consejo Superior de Investigaciones Científicas. Instituto de Investigación en Ciencias de la Alimentación; España. Universidad Autónoma de Madrid; España
Fil: Zárate, Gabriela. Universidad San Pablo Tucumán; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Materia
B-GALACTOSIDASA
LACTIC ACID BACTERIA
TRANSGALACTOSYLATION
OLIGOSACCHARIDES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/111589
Acceder
id CONICETDig_9de42941c46efcbe1d24d906d3fe0631
oai_identifier_str oai:ri.conicet.gov.ar:11336/111589
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Prebiotic galactooligosaccharides production from lactose and lactulose by Lactobacillus delbrueckii subsp. bulgaricus CRL450Fara, María AgustinaSabater, Carlos Edgardo AlejandroPalacios, Jorge MartinRequena, TeresaMontilla Corredera, AntoniaZárate, GabrielaB-GALACTOSIDASALACTIC ACID BACTERIATRANSGALACTOSYLATIONOLIGOSACCHARIDEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Galactooligosaccharides (GOS) are useful dietary ingredients recognized worldwide as prebiotics. In the present study, we evaluated the β-galactosidase (β-gal) activity of a panel of lactic acid bacteria (LAB) in order to select strains for the synthesis of oligosaccharides from lactose (GOS) and lactulose (OsLu) with a potential prebiotic effect. Fifteen strains out of 20 were able to grow on lactose and showed β-gal activities between 0.03 and 2.06 U mg−1, whereas eleven were able to synthesize GOS. Lactobacillus delbrueckii subsp. bulgaricus CRL450, the strain with the highest β-gal activity, synthesized a maximum of 41.3% GOS and 21.0% OsLu from lactose and lactulose, respectively, with β-(1 → 6) and secondary β-(1 → 3) linkages. When these compounds were tested without purifying, as carbon sources for the development of recognized probiotics and the producer strain, high growth was observed compared to non-prebiotic sugars like glucose and lactose. When the purified oligosaccharides were tested, the bacterial growth decreased, but the microorganisms displayed metabolic activity evidenced by the consumption of carbohydrates and the production of lactic acid. Additionally, the purified oligosaccharides demonstrated a bifidogenic effect. The obtained results support the potential of L. delbrueckii subsp. bulgaricus CRL450 for the production of the prebiotics GOS and OsLu and encourage the optimization of their synthesis for the design of new functional food ingredients.Fil: Fara, María Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Sabater, Carlos Edgardo Alejandro. Consejo Superior de Investigaciones Científicas. Instituto de Investigación en Ciencias de la Alimentación; España. Universidad Autónoma de Madrid; EspañaFil: Palacios, Jorge Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Requena, Teresa. Consejo Superior de Investigaciones Científicas. Instituto de Investigación en Ciencias de la Alimentación; España. Universidad Autónoma de Madrid; EspañaFil: Montilla Corredera, Antonia. Consejo Superior de Investigaciones Científicas. Instituto de Investigación en Ciencias de la Alimentación; España. Universidad Autónoma de Madrid; EspañaFil: Zárate, Gabriela. Universidad San Pablo Tucumán; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaRoyal Society of Chemistry2020-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/111589Fara, María Agustina; Sabater, Carlos Edgardo Alejandro; Palacios, Jorge Martin; Requena, Teresa; Montilla Corredera, Antonia; et al.; Prebiotic galactooligosaccharides production from lactose and lactulose by Lactobacillus delbrueckii subsp. bulgaricus CRL450; Royal Society of Chemistry; Food & Function; 7; 6-2020; 1-122042-64962042-650XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/Content/ArticleLanding/2020/FO/D0FO00942Cinfo:eu-repo/semantics/altIdentifier/doi/10.1039/D0FO00942Cinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:58:25Zoai:ri.conicet.gov.ar:11336/111589instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:58:26.275CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Prebiotic galactooligosaccharides production from lactose and lactulose by Lactobacillus delbrueckii subsp. bulgaricus CRL450
title Prebiotic galactooligosaccharides production from lactose and lactulose by Lactobacillus delbrueckii subsp. bulgaricus CRL450
spellingShingle Prebiotic galactooligosaccharides production from lactose and lactulose by Lactobacillus delbrueckii subsp. bulgaricus CRL450
Fara, María Agustina
B-GALACTOSIDASA
LACTIC ACID BACTERIA
TRANSGALACTOSYLATION
OLIGOSACCHARIDES
title_short Prebiotic galactooligosaccharides production from lactose and lactulose by Lactobacillus delbrueckii subsp. bulgaricus CRL450
title_full Prebiotic galactooligosaccharides production from lactose and lactulose by Lactobacillus delbrueckii subsp. bulgaricus CRL450
title_fullStr Prebiotic galactooligosaccharides production from lactose and lactulose by Lactobacillus delbrueckii subsp. bulgaricus CRL450
title_full_unstemmed Prebiotic galactooligosaccharides production from lactose and lactulose by Lactobacillus delbrueckii subsp. bulgaricus CRL450
title_sort Prebiotic galactooligosaccharides production from lactose and lactulose by Lactobacillus delbrueckii subsp. bulgaricus CRL450
dc.creator.none.fl_str_mv Fara, María Agustina
Sabater, Carlos Edgardo Alejandro
Palacios, Jorge Martin
Requena, Teresa
Montilla Corredera, Antonia
Zárate, Gabriela
author Fara, María Agustina
author_facet Fara, María Agustina
Sabater, Carlos Edgardo Alejandro
Palacios, Jorge Martin
Requena, Teresa
Montilla Corredera, Antonia
Zárate, Gabriela
author_role author
author2 Sabater, Carlos Edgardo Alejandro
Palacios, Jorge Martin
Requena, Teresa
Montilla Corredera, Antonia
Zárate, Gabriela
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv B-GALACTOSIDASA
LACTIC ACID BACTERIA
TRANSGALACTOSYLATION
OLIGOSACCHARIDES
topic B-GALACTOSIDASA
LACTIC ACID BACTERIA
TRANSGALACTOSYLATION
OLIGOSACCHARIDES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Galactooligosaccharides (GOS) are useful dietary ingredients recognized worldwide as prebiotics. In the present study, we evaluated the β-galactosidase (β-gal) activity of a panel of lactic acid bacteria (LAB) in order to select strains for the synthesis of oligosaccharides from lactose (GOS) and lactulose (OsLu) with a potential prebiotic effect. Fifteen strains out of 20 were able to grow on lactose and showed β-gal activities between 0.03 and 2.06 U mg−1, whereas eleven were able to synthesize GOS. Lactobacillus delbrueckii subsp. bulgaricus CRL450, the strain with the highest β-gal activity, synthesized a maximum of 41.3% GOS and 21.0% OsLu from lactose and lactulose, respectively, with β-(1 → 6) and secondary β-(1 → 3) linkages. When these compounds were tested without purifying, as carbon sources for the development of recognized probiotics and the producer strain, high growth was observed compared to non-prebiotic sugars like glucose and lactose. When the purified oligosaccharides were tested, the bacterial growth decreased, but the microorganisms displayed metabolic activity evidenced by the consumption of carbohydrates and the production of lactic acid. Additionally, the purified oligosaccharides demonstrated a bifidogenic effect. The obtained results support the potential of L. delbrueckii subsp. bulgaricus CRL450 for the production of the prebiotics GOS and OsLu and encourage the optimization of their synthesis for the design of new functional food ingredients.
Fil: Fara, María Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Sabater, Carlos Edgardo Alejandro. Consejo Superior de Investigaciones Científicas. Instituto de Investigación en Ciencias de la Alimentación; España. Universidad Autónoma de Madrid; España
Fil: Palacios, Jorge Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Requena, Teresa. Consejo Superior de Investigaciones Científicas. Instituto de Investigación en Ciencias de la Alimentación; España. Universidad Autónoma de Madrid; España
Fil: Montilla Corredera, Antonia. Consejo Superior de Investigaciones Científicas. Instituto de Investigación en Ciencias de la Alimentación; España. Universidad Autónoma de Madrid; España
Fil: Zárate, Gabriela. Universidad San Pablo Tucumán; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
description Galactooligosaccharides (GOS) are useful dietary ingredients recognized worldwide as prebiotics. In the present study, we evaluated the β-galactosidase (β-gal) activity of a panel of lactic acid bacteria (LAB) in order to select strains for the synthesis of oligosaccharides from lactose (GOS) and lactulose (OsLu) with a potential prebiotic effect. Fifteen strains out of 20 were able to grow on lactose and showed β-gal activities between 0.03 and 2.06 U mg−1, whereas eleven were able to synthesize GOS. Lactobacillus delbrueckii subsp. bulgaricus CRL450, the strain with the highest β-gal activity, synthesized a maximum of 41.3% GOS and 21.0% OsLu from lactose and lactulose, respectively, with β-(1 → 6) and secondary β-(1 → 3) linkages. When these compounds were tested without purifying, as carbon sources for the development of recognized probiotics and the producer strain, high growth was observed compared to non-prebiotic sugars like glucose and lactose. When the purified oligosaccharides were tested, the bacterial growth decreased, but the microorganisms displayed metabolic activity evidenced by the consumption of carbohydrates and the production of lactic acid. Additionally, the purified oligosaccharides demonstrated a bifidogenic effect. The obtained results support the potential of L. delbrueckii subsp. bulgaricus CRL450 for the production of the prebiotics GOS and OsLu and encourage the optimization of their synthesis for the design of new functional food ingredients.
publishDate 2020
dc.date.none.fl_str_mv 2020-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/111589
Fara, María Agustina; Sabater, Carlos Edgardo Alejandro; Palacios, Jorge Martin; Requena, Teresa; Montilla Corredera, Antonia; et al.; Prebiotic galactooligosaccharides production from lactose and lactulose by Lactobacillus delbrueckii subsp. bulgaricus CRL450; Royal Society of Chemistry; Food & Function; 7; 6-2020; 1-12
2042-6496
2042-650X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/111589
identifier_str_mv Fara, María Agustina; Sabater, Carlos Edgardo Alejandro; Palacios, Jorge Martin; Requena, Teresa; Montilla Corredera, Antonia; et al.; Prebiotic galactooligosaccharides production from lactose and lactulose by Lactobacillus delbrueckii subsp. bulgaricus CRL450; Royal Society of Chemistry; Food & Function; 7; 6-2020; 1-12
2042-6496
2042-650X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/Content/ArticleLanding/2020/FO/D0FO00942C
info:eu-repo/semantics/altIdentifier/doi/10.1039/D0FO00942C
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269519987343360
score 13.13397

Publicaciones similares