Glutamate uptake in Lactobacillus delbrueckii subsp. bulgaricus CNRZ 208 and its enhancement by a combination of Mn2+ and Mg2+
- Autores
- Savoy, Graciela; Foucaud-Scheunemann, Catherine; Ferchichi, Mohamed; Hemme, Denis
- Año de publicación
- 2002
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Aims: To demonstrate the mechanism of glutamate uptake in the dairy strain Lactobacillus delbrueckii subsp. bulgaricus CNRZ 208, and to characterize key aspects of the system. Methods and Results: Glutamate uptake proceeded via an active transport system requiring an exogenous source of energy. The system also transported aspartate and glutamine. It was unique, with a Kt of 2.8 μmoll-1 and a Vmax of 900 μmol s-1 (g dry weight)-1. The activity was optimal at pH 7.3 and 50°C, was independent of the glutamate charge, and was enhanced by Mn2+ + Mg2+ in combination. Inhibition of the activity by uncouplers and ionophores showed that transport was driven by an ATP-dependent mechanism involving the proton-motive force. This inhibition was partially abolished in the presence of both Mn2+ and Mg2+. Conclusions: We demonstrated for the first time that an active transport system governs the uptake of the essential amino acid glutamate in Lact. delbrueckii subsp. bulgaricus CNRZ 208, the activity of which is enhanced by a combination of Mn2+ and Mg2+. Significance and Impact of the Study: The potential of the findings is discussed with reference to the growth of Lact. delbrueckii subsp. bulgaricus in mixed-strain cultures for the dairy industry.
Fil: Savoy, Graciela. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Foucaud-Scheunemann, Catherine. Unité de Recherches Laitières Et Génétique Appliquée, I; Francia
Fil: Ferchichi, Mohamed. Unité de Recherches Laitières Et Génétique Appliquée, I; Francia
Fil: Hemme, Denis. Unité de Recherches Laitières Et Génétique Appliquée, I; Francia - Materia
-
Glutamate Uptake
Lactobacillus Delbrueckii Subsp. Bulgaricus
Amino Acid Transport - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/57275
Ver los metadatos del registro completo
id |
CONICETDig_1b67c58cd39d0b6068b08a83f8ac7228 |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/57275 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Glutamate uptake in Lactobacillus delbrueckii subsp. bulgaricus CNRZ 208 and its enhancement by a combination of Mn2+ and Mg2+Savoy, GracielaFoucaud-Scheunemann, CatherineFerchichi, MohamedHemme, DenisGlutamate UptakeLactobacillus Delbrueckii Subsp. BulgaricusAmino Acid TransportAims: To demonstrate the mechanism of glutamate uptake in the dairy strain Lactobacillus delbrueckii subsp. bulgaricus CNRZ 208, and to characterize key aspects of the system. Methods and Results: Glutamate uptake proceeded via an active transport system requiring an exogenous source of energy. The system also transported aspartate and glutamine. It was unique, with a Kt of 2.8 μmoll-1 and a Vmax of 900 μmol s-1 (g dry weight)-1. The activity was optimal at pH 7.3 and 50°C, was independent of the glutamate charge, and was enhanced by Mn2+ + Mg2+ in combination. Inhibition of the activity by uncouplers and ionophores showed that transport was driven by an ATP-dependent mechanism involving the proton-motive force. This inhibition was partially abolished in the presence of both Mn2+ and Mg2+. Conclusions: We demonstrated for the first time that an active transport system governs the uptake of the essential amino acid glutamate in Lact. delbrueckii subsp. bulgaricus CNRZ 208, the activity of which is enhanced by a combination of Mn2+ and Mg2+. Significance and Impact of the Study: The potential of the findings is discussed with reference to the growth of Lact. delbrueckii subsp. bulgaricus in mixed-strain cultures for the dairy industry.Fil: Savoy, Graciela. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Foucaud-Scheunemann, Catherine. Unité de Recherches Laitières Et Génétique Appliquée, I; FranciaFil: Ferchichi, Mohamed. Unité de Recherches Laitières Et Génétique Appliquée, I; FranciaFil: Hemme, Denis. Unité de Recherches Laitières Et Génétique Appliquée, I; FranciaWiley Blackwell Publishing, Inc2002-10-17info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/57275Savoy, Graciela; Foucaud-Scheunemann, Catherine; Ferchichi, Mohamed; Hemme, Denis; Glutamate uptake in Lactobacillus delbrueckii subsp. bulgaricus CNRZ 208 and its enhancement by a combination of Mn2+ and Mg2+; Wiley Blackwell Publishing, Inc; Letters in Applied Microbiology; 35; 5; 17-10-2002; 428-4320266-82541472-765XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1046/j.1472-765X.2002.01208.xinfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1046/j.1472-765X.2002.01208.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:56:12Zoai:ri.conicet.gov.ar:11336/57275instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:56:12.371CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Glutamate uptake in Lactobacillus delbrueckii subsp. bulgaricus CNRZ 208 and its enhancement by a combination of Mn2+ and Mg2+ |
title |
Glutamate uptake in Lactobacillus delbrueckii subsp. bulgaricus CNRZ 208 and its enhancement by a combination of Mn2+ and Mg2+ |
spellingShingle |
Glutamate uptake in Lactobacillus delbrueckii subsp. bulgaricus CNRZ 208 and its enhancement by a combination of Mn2+ and Mg2+ Savoy, Graciela Glutamate Uptake Lactobacillus Delbrueckii Subsp. Bulgaricus Amino Acid Transport |
title_short |
Glutamate uptake in Lactobacillus delbrueckii subsp. bulgaricus CNRZ 208 and its enhancement by a combination of Mn2+ and Mg2+ |
title_full |
Glutamate uptake in Lactobacillus delbrueckii subsp. bulgaricus CNRZ 208 and its enhancement by a combination of Mn2+ and Mg2+ |
title_fullStr |
Glutamate uptake in Lactobacillus delbrueckii subsp. bulgaricus CNRZ 208 and its enhancement by a combination of Mn2+ and Mg2+ |
title_full_unstemmed |
Glutamate uptake in Lactobacillus delbrueckii subsp. bulgaricus CNRZ 208 and its enhancement by a combination of Mn2+ and Mg2+ |
title_sort |
Glutamate uptake in Lactobacillus delbrueckii subsp. bulgaricus CNRZ 208 and its enhancement by a combination of Mn2+ and Mg2+ |
dc.creator.none.fl_str_mv |
Savoy, Graciela Foucaud-Scheunemann, Catherine Ferchichi, Mohamed Hemme, Denis |
author |
Savoy, Graciela |
author_facet |
Savoy, Graciela Foucaud-Scheunemann, Catherine Ferchichi, Mohamed Hemme, Denis |
author_role |
author |
author2 |
Foucaud-Scheunemann, Catherine Ferchichi, Mohamed Hemme, Denis |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Glutamate Uptake Lactobacillus Delbrueckii Subsp. Bulgaricus Amino Acid Transport |
topic |
Glutamate Uptake Lactobacillus Delbrueckii Subsp. Bulgaricus Amino Acid Transport |
dc.description.none.fl_txt_mv |
Aims: To demonstrate the mechanism of glutamate uptake in the dairy strain Lactobacillus delbrueckii subsp. bulgaricus CNRZ 208, and to characterize key aspects of the system. Methods and Results: Glutamate uptake proceeded via an active transport system requiring an exogenous source of energy. The system also transported aspartate and glutamine. It was unique, with a Kt of 2.8 μmoll-1 and a Vmax of 900 μmol s-1 (g dry weight)-1. The activity was optimal at pH 7.3 and 50°C, was independent of the glutamate charge, and was enhanced by Mn2+ + Mg2+ in combination. Inhibition of the activity by uncouplers and ionophores showed that transport was driven by an ATP-dependent mechanism involving the proton-motive force. This inhibition was partially abolished in the presence of both Mn2+ and Mg2+. Conclusions: We demonstrated for the first time that an active transport system governs the uptake of the essential amino acid glutamate in Lact. delbrueckii subsp. bulgaricus CNRZ 208, the activity of which is enhanced by a combination of Mn2+ and Mg2+. Significance and Impact of the Study: The potential of the findings is discussed with reference to the growth of Lact. delbrueckii subsp. bulgaricus in mixed-strain cultures for the dairy industry. Fil: Savoy, Graciela. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Foucaud-Scheunemann, Catherine. Unité de Recherches Laitières Et Génétique Appliquée, I; Francia Fil: Ferchichi, Mohamed. Unité de Recherches Laitières Et Génétique Appliquée, I; Francia Fil: Hemme, Denis. Unité de Recherches Laitières Et Génétique Appliquée, I; Francia |
description |
Aims: To demonstrate the mechanism of glutamate uptake in the dairy strain Lactobacillus delbrueckii subsp. bulgaricus CNRZ 208, and to characterize key aspects of the system. Methods and Results: Glutamate uptake proceeded via an active transport system requiring an exogenous source of energy. The system also transported aspartate and glutamine. It was unique, with a Kt of 2.8 μmoll-1 and a Vmax of 900 μmol s-1 (g dry weight)-1. The activity was optimal at pH 7.3 and 50°C, was independent of the glutamate charge, and was enhanced by Mn2+ + Mg2+ in combination. Inhibition of the activity by uncouplers and ionophores showed that transport was driven by an ATP-dependent mechanism involving the proton-motive force. This inhibition was partially abolished in the presence of both Mn2+ and Mg2+. Conclusions: We demonstrated for the first time that an active transport system governs the uptake of the essential amino acid glutamate in Lact. delbrueckii subsp. bulgaricus CNRZ 208, the activity of which is enhanced by a combination of Mn2+ and Mg2+. Significance and Impact of the Study: The potential of the findings is discussed with reference to the growth of Lact. delbrueckii subsp. bulgaricus in mixed-strain cultures for the dairy industry. |
publishDate |
2002 |
dc.date.none.fl_str_mv |
2002-10-17 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/57275 Savoy, Graciela; Foucaud-Scheunemann, Catherine; Ferchichi, Mohamed; Hemme, Denis; Glutamate uptake in Lactobacillus delbrueckii subsp. bulgaricus CNRZ 208 and its enhancement by a combination of Mn2+ and Mg2+; Wiley Blackwell Publishing, Inc; Letters in Applied Microbiology; 35; 5; 17-10-2002; 428-432 0266-8254 1472-765X CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/57275 |
identifier_str_mv |
Savoy, Graciela; Foucaud-Scheunemann, Catherine; Ferchichi, Mohamed; Hemme, Denis; Glutamate uptake in Lactobacillus delbrueckii subsp. bulgaricus CNRZ 208 and its enhancement by a combination of Mn2+ and Mg2+; Wiley Blackwell Publishing, Inc; Letters in Applied Microbiology; 35; 5; 17-10-2002; 428-432 0266-8254 1472-765X CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1046/j.1472-765X.2002.01208.x info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1046/j.1472-765X.2002.01208.x |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1842269390155808768 |
score |
13.13397 |