Why Is Less Cationic Lipid Required To Prepare Lipoplexes from Plasmid DNA than Linear DNA in Gene Therapy?

Autores
Muñoz Úbeda, Mónica; Misra, Santosh K.; Barrán Berdón, Ana L.; Aicart Ramos, Clara; Sierra, Maria Belen; Biswas, Joydeep; Kondaiah, Paturu; Junquera, Elena; Bhattacharya, Santanu; Aicart, Emilio
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The most important objective of the present study was to explain why cationic lipid (CL)-mediated delivery of plasmid DNA (pDNA) is better than that of linear DNA in gene therapy, a question that, until now, has remained unanswered. Herein for the first time we experimentally show that for different types of CLs, pDNA, in contrast to linear DNA, is compacted with a large amount of its counterions, yielding a lower effective negative charge. This feature has been confirmed through a number of physicochemical and biochemical investigations. This is significant for both in vitro and in vivo transfection studies. For an effective DNA transfection, the lower the amount of the CL, the lower is the cytotoxicity. The study also points out that it is absolutely necessary to consider both effective charge ratios between CL and pDNA and effective pDNA charges, which can be determined from physicochemical experiments.
Fil: Muñoz Úbeda, Mónica. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España
Fil: Misra, Santosh K.. Indian Institute of Science; India
Fil: Barrán Berdón, Ana L.. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España
Fil: Aicart Ramos, Clara. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España
Fil: Sierra, Maria Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Biswas, Joydeep. Indian Institute of Science; India
Fil: Kondaiah, Paturu. Indian Institute of Science; India
Fil: Junquera, Elena. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España
Fil: Bhattacharya, Santanu. Indian Institute of Science; India
Fil: Aicart, Emilio. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España
Materia
GENE THERAPY
DNA
LIPOPLEXES
LIPOSOMES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/96961
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/96961
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Why Is Less Cationic Lipid Required To Prepare Lipoplexes from Plasmid DNA than Linear DNA in Gene Therapy?Muñoz Úbeda, MónicaMisra, Santosh K.Barrán Berdón, Ana L.Aicart Ramos, ClaraSierra, Maria BelenBiswas, JoydeepKondaiah, PaturuJunquera, ElenaBhattacharya, SantanuAicart, EmilioGENE THERAPYDNALIPOPLEXESLIPOSOMEShttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2The most important objective of the present study was to explain why cationic lipid (CL)-mediated delivery of plasmid DNA (pDNA) is better than that of linear DNA in gene therapy, a question that, until now, has remained unanswered. Herein for the first time we experimentally show that for different types of CLs, pDNA, in contrast to linear DNA, is compacted with a large amount of its counterions, yielding a lower effective negative charge. This feature has been confirmed through a number of physicochemical and biochemical investigations. This is significant for both in vitro and in vivo transfection studies. For an effective DNA transfection, the lower the amount of the CL, the lower is the cytotoxicity. The study also points out that it is absolutely necessary to consider both effective charge ratios between CL and pDNA and effective pDNA charges, which can be determined from physicochemical experiments.Fil: Muñoz Úbeda, Mónica. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; EspañaFil: Misra, Santosh K.. Indian Institute of Science; IndiaFil: Barrán Berdón, Ana L.. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; EspañaFil: Aicart Ramos, Clara. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; EspañaFil: Sierra, Maria Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Biswas, Joydeep. Indian Institute of Science; IndiaFil: Kondaiah, Paturu. Indian Institute of Science; IndiaFil: Junquera, Elena. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; EspañaFil: Bhattacharya, Santanu. Indian Institute of Science; IndiaFil: Aicart, Emilio. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; EspañaAmerican Chemical Society2011-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/96961Muñoz Úbeda, Mónica; Misra, Santosh K.; Barrán Berdón, Ana L.; Aicart Ramos, Clara; Sierra, Maria Belen; et al.; Why Is Less Cationic Lipid Required To Prepare Lipoplexes from Plasmid DNA than Linear DNA in Gene Therapy?; American Chemical Society; Journal of the American Chemical Society; 133; 45; 11-2011; 18014-180170002-7863CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/abs/10.1021/ja204693finfo:eu-repo/semantics/altIdentifier/doi/10.1021/ja204693finfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:17:54Zoai:ri.conicet.gov.ar:11336/96961instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:17:54.973CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Why Is Less Cationic Lipid Required To Prepare Lipoplexes from Plasmid DNA than Linear DNA in Gene Therapy?
title Why Is Less Cationic Lipid Required To Prepare Lipoplexes from Plasmid DNA than Linear DNA in Gene Therapy?
spellingShingle Why Is Less Cationic Lipid Required To Prepare Lipoplexes from Plasmid DNA than Linear DNA in Gene Therapy?
Muñoz Úbeda, Mónica
GENE THERAPY
DNA
LIPOPLEXES
LIPOSOMES
title_short Why Is Less Cationic Lipid Required To Prepare Lipoplexes from Plasmid DNA than Linear DNA in Gene Therapy?
title_full Why Is Less Cationic Lipid Required To Prepare Lipoplexes from Plasmid DNA than Linear DNA in Gene Therapy?
title_fullStr Why Is Less Cationic Lipid Required To Prepare Lipoplexes from Plasmid DNA than Linear DNA in Gene Therapy?
title_full_unstemmed Why Is Less Cationic Lipid Required To Prepare Lipoplexes from Plasmid DNA than Linear DNA in Gene Therapy?
title_sort Why Is Less Cationic Lipid Required To Prepare Lipoplexes from Plasmid DNA than Linear DNA in Gene Therapy?
dc.creator.none.fl_str_mv Muñoz Úbeda, Mónica
Misra, Santosh K.
Barrán Berdón, Ana L.
Aicart Ramos, Clara
Sierra, Maria Belen
Biswas, Joydeep
Kondaiah, Paturu
Junquera, Elena
Bhattacharya, Santanu
Aicart, Emilio
author Muñoz Úbeda, Mónica
author_facet Muñoz Úbeda, Mónica
Misra, Santosh K.
Barrán Berdón, Ana L.
Aicart Ramos, Clara
Sierra, Maria Belen
Biswas, Joydeep
Kondaiah, Paturu
Junquera, Elena
Bhattacharya, Santanu
Aicart, Emilio
author_role author
author2 Misra, Santosh K.
Barrán Berdón, Ana L.
Aicart Ramos, Clara
Sierra, Maria Belen
Biswas, Joydeep
Kondaiah, Paturu
Junquera, Elena
Bhattacharya, Santanu
Aicart, Emilio
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv GENE THERAPY
DNA
LIPOPLEXES
LIPOSOMES
topic GENE THERAPY
DNA
LIPOPLEXES
LIPOSOMES
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The most important objective of the present study was to explain why cationic lipid (CL)-mediated delivery of plasmid DNA (pDNA) is better than that of linear DNA in gene therapy, a question that, until now, has remained unanswered. Herein for the first time we experimentally show that for different types of CLs, pDNA, in contrast to linear DNA, is compacted with a large amount of its counterions, yielding a lower effective negative charge. This feature has been confirmed through a number of physicochemical and biochemical investigations. This is significant for both in vitro and in vivo transfection studies. For an effective DNA transfection, the lower the amount of the CL, the lower is the cytotoxicity. The study also points out that it is absolutely necessary to consider both effective charge ratios between CL and pDNA and effective pDNA charges, which can be determined from physicochemical experiments.
Fil: Muñoz Úbeda, Mónica. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España
Fil: Misra, Santosh K.. Indian Institute of Science; India
Fil: Barrán Berdón, Ana L.. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España
Fil: Aicart Ramos, Clara. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España
Fil: Sierra, Maria Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Biswas, Joydeep. Indian Institute of Science; India
Fil: Kondaiah, Paturu. Indian Institute of Science; India
Fil: Junquera, Elena. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España
Fil: Bhattacharya, Santanu. Indian Institute of Science; India
Fil: Aicart, Emilio. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España
description The most important objective of the present study was to explain why cationic lipid (CL)-mediated delivery of plasmid DNA (pDNA) is better than that of linear DNA in gene therapy, a question that, until now, has remained unanswered. Herein for the first time we experimentally show that for different types of CLs, pDNA, in contrast to linear DNA, is compacted with a large amount of its counterions, yielding a lower effective negative charge. This feature has been confirmed through a number of physicochemical and biochemical investigations. This is significant for both in vitro and in vivo transfection studies. For an effective DNA transfection, the lower the amount of the CL, the lower is the cytotoxicity. The study also points out that it is absolutely necessary to consider both effective charge ratios between CL and pDNA and effective pDNA charges, which can be determined from physicochemical experiments.
publishDate 2011
dc.date.none.fl_str_mv 2011-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/96961
Muñoz Úbeda, Mónica; Misra, Santosh K.; Barrán Berdón, Ana L.; Aicart Ramos, Clara; Sierra, Maria Belen; et al.; Why Is Less Cationic Lipid Required To Prepare Lipoplexes from Plasmid DNA than Linear DNA in Gene Therapy?; American Chemical Society; Journal of the American Chemical Society; 133; 45; 11-2011; 18014-18017
0002-7863
CONICET Digital
CONICET
url http://hdl.handle.net/11336/96961
identifier_str_mv Muñoz Úbeda, Mónica; Misra, Santosh K.; Barrán Berdón, Ana L.; Aicart Ramos, Clara; Sierra, Maria Belen; et al.; Why Is Less Cationic Lipid Required To Prepare Lipoplexes from Plasmid DNA than Linear DNA in Gene Therapy?; American Chemical Society; Journal of the American Chemical Society; 133; 45; 11-2011; 18014-18017
0002-7863
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/abs/10.1021/ja204693f
info:eu-repo/semantics/altIdentifier/doi/10.1021/ja204693f
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842980979397885952
score 12.993085

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