Intracellular trafficking of cationic liposome/DNA complexes in living cells

Autores
Coppola, Stefano; Estrada, Laura Cecilia; Digman, Michelle A.; Pozzi, Daniela; Cardarelli, Francesco; Gratton, Enrico; Caracciolo, Giulio
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Three-dimensional single-particle tracking (SPT) was used to calculate the mean square displacement (MSD) and the diffusion coefficients of multicomponent cationic liposome-DNA complexes (lipoplexes) in CHO-K1 living cells. In untreated (NT) control cells, we found that the intracellular lipoplex motion was either directed or Brownian with active transportation being definitely more frequent (more than 70%) than Brownian diffusion. The MSD analysis was supported by the calculation of the three-dimensional asphericity, A 3, which was close to unity, denoting the preponderant occurrence of movement along a direction. To elucidate the role of the cytoskeleton structure in the lipoplex trafficking, cells were treated with cytoskeleton (actin microfilaments and microtubules) polymerization inhibitors (latrunculin B and nocodazole, respectively). When cells were treated with inhibitors, the lipoplex movement tended towards a random walk at the expense of directed motion. The disassembly of microtubules had a stronger effect on the reduction of directional movement than that of actin microfilaments. Relevance of the results for enhanced gene delivery is discussed.
Fil: Coppola, Stefano. Università di Roma; Italia
Fil: Estrada, Laura Cecilia. University of California; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Digman, Michelle A.. University of California; Estados Unidos
Fil: Pozzi, Daniela. Università di Roma; Italia
Fil: Cardarelli, Francesco. Istituto Italiano di Tecnologia; Italia
Fil: Gratton, Enrico. University of California; Estados Unidos
Fil: Caracciolo, Giulio. Università di Roma; Italia
Materia
PARTICLE TRACKING
LIPOPLEXES
GENE DELIVERY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/199318
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Intracellular trafficking of cationic liposome/DNA complexes in living cellsCoppola, StefanoEstrada, Laura CeciliaDigman, Michelle A.Pozzi, DanielaCardarelli, FrancescoGratton, EnricoCaracciolo, GiulioPARTICLE TRACKINGLIPOPLEXESGENE DELIVERYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Three-dimensional single-particle tracking (SPT) was used to calculate the mean square displacement (MSD) and the diffusion coefficients of multicomponent cationic liposome-DNA complexes (lipoplexes) in CHO-K1 living cells. In untreated (NT) control cells, we found that the intracellular lipoplex motion was either directed or Brownian with active transportation being definitely more frequent (more than 70%) than Brownian diffusion. The MSD analysis was supported by the calculation of the three-dimensional asphericity, A 3, which was close to unity, denoting the preponderant occurrence of movement along a direction. To elucidate the role of the cytoskeleton structure in the lipoplex trafficking, cells were treated with cytoskeleton (actin microfilaments and microtubules) polymerization inhibitors (latrunculin B and nocodazole, respectively). When cells were treated with inhibitors, the lipoplex movement tended towards a random walk at the expense of directed motion. The disassembly of microtubules had a stronger effect on the reduction of directional movement than that of actin microfilaments. Relevance of the results for enhanced gene delivery is discussed.Fil: Coppola, Stefano. Università di Roma; ItaliaFil: Estrada, Laura Cecilia. University of California; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Digman, Michelle A.. University of California; Estados UnidosFil: Pozzi, Daniela. Università di Roma; ItaliaFil: Cardarelli, Francesco. Istituto Italiano di Tecnologia; ItaliaFil: Gratton, Enrico. University of California; Estados UnidosFil: Caracciolo, Giulio. Università di Roma; ItaliaRoyal Society of Chemistry2012-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/199318Coppola, Stefano; Estrada, Laura Cecilia; Digman, Michelle A.; Pozzi, Daniela; Cardarelli, Francesco; et al.; Intracellular trafficking of cationic liposome/DNA complexes in living cells; Royal Society of Chemistry; Soft Matter; 8; 30; 8-2012; 7919-79271744-683XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/Content/ArticleLanding/2012/SM/c2sm25532dinfo:eu-repo/semantics/altIdentifier/doi/10.1039/C2SM25532Dinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:31:33Zoai:ri.conicet.gov.ar:11336/199318instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:31:33.742CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Intracellular trafficking of cationic liposome/DNA complexes in living cells
title Intracellular trafficking of cationic liposome/DNA complexes in living cells
spellingShingle Intracellular trafficking of cationic liposome/DNA complexes in living cells
Coppola, Stefano
PARTICLE TRACKING
LIPOPLEXES
GENE DELIVERY
title_short Intracellular trafficking of cationic liposome/DNA complexes in living cells
title_full Intracellular trafficking of cationic liposome/DNA complexes in living cells
title_fullStr Intracellular trafficking of cationic liposome/DNA complexes in living cells
title_full_unstemmed Intracellular trafficking of cationic liposome/DNA complexes in living cells
title_sort Intracellular trafficking of cationic liposome/DNA complexes in living cells
dc.creator.none.fl_str_mv Coppola, Stefano
Estrada, Laura Cecilia
Digman, Michelle A.
Pozzi, Daniela
Cardarelli, Francesco
Gratton, Enrico
Caracciolo, Giulio
author Coppola, Stefano
author_facet Coppola, Stefano
Estrada, Laura Cecilia
Digman, Michelle A.
Pozzi, Daniela
Cardarelli, Francesco
Gratton, Enrico
Caracciolo, Giulio
author_role author
author2 Estrada, Laura Cecilia
Digman, Michelle A.
Pozzi, Daniela
Cardarelli, Francesco
Gratton, Enrico
Caracciolo, Giulio
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv PARTICLE TRACKING
LIPOPLEXES
GENE DELIVERY
topic PARTICLE TRACKING
LIPOPLEXES
GENE DELIVERY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Three-dimensional single-particle tracking (SPT) was used to calculate the mean square displacement (MSD) and the diffusion coefficients of multicomponent cationic liposome-DNA complexes (lipoplexes) in CHO-K1 living cells. In untreated (NT) control cells, we found that the intracellular lipoplex motion was either directed or Brownian with active transportation being definitely more frequent (more than 70%) than Brownian diffusion. The MSD analysis was supported by the calculation of the three-dimensional asphericity, A 3, which was close to unity, denoting the preponderant occurrence of movement along a direction. To elucidate the role of the cytoskeleton structure in the lipoplex trafficking, cells were treated with cytoskeleton (actin microfilaments and microtubules) polymerization inhibitors (latrunculin B and nocodazole, respectively). When cells were treated with inhibitors, the lipoplex movement tended towards a random walk at the expense of directed motion. The disassembly of microtubules had a stronger effect on the reduction of directional movement than that of actin microfilaments. Relevance of the results for enhanced gene delivery is discussed.
Fil: Coppola, Stefano. Università di Roma; Italia
Fil: Estrada, Laura Cecilia. University of California; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Digman, Michelle A.. University of California; Estados Unidos
Fil: Pozzi, Daniela. Università di Roma; Italia
Fil: Cardarelli, Francesco. Istituto Italiano di Tecnologia; Italia
Fil: Gratton, Enrico. University of California; Estados Unidos
Fil: Caracciolo, Giulio. Università di Roma; Italia
description Three-dimensional single-particle tracking (SPT) was used to calculate the mean square displacement (MSD) and the diffusion coefficients of multicomponent cationic liposome-DNA complexes (lipoplexes) in CHO-K1 living cells. In untreated (NT) control cells, we found that the intracellular lipoplex motion was either directed or Brownian with active transportation being definitely more frequent (more than 70%) than Brownian diffusion. The MSD analysis was supported by the calculation of the three-dimensional asphericity, A 3, which was close to unity, denoting the preponderant occurrence of movement along a direction. To elucidate the role of the cytoskeleton structure in the lipoplex trafficking, cells were treated with cytoskeleton (actin microfilaments and microtubules) polymerization inhibitors (latrunculin B and nocodazole, respectively). When cells were treated with inhibitors, the lipoplex movement tended towards a random walk at the expense of directed motion. The disassembly of microtubules had a stronger effect on the reduction of directional movement than that of actin microfilaments. Relevance of the results for enhanced gene delivery is discussed.
publishDate 2012
dc.date.none.fl_str_mv 2012-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/199318
Coppola, Stefano; Estrada, Laura Cecilia; Digman, Michelle A.; Pozzi, Daniela; Cardarelli, Francesco; et al.; Intracellular trafficking of cationic liposome/DNA complexes in living cells; Royal Society of Chemistry; Soft Matter; 8; 30; 8-2012; 7919-7927
1744-683X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/199318
identifier_str_mv Coppola, Stefano; Estrada, Laura Cecilia; Digman, Michelle A.; Pozzi, Daniela; Cardarelli, Francesco; et al.; Intracellular trafficking of cationic liposome/DNA complexes in living cells; Royal Society of Chemistry; Soft Matter; 8; 30; 8-2012; 7919-7927
1744-683X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/Content/ArticleLanding/2012/SM/c2sm25532d
info:eu-repo/semantics/altIdentifier/doi/10.1039/C2SM25532D
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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