Chronic stress and its effects on adrenal cortex apoptosis in pregnant rats

Autores
Bozzo, Aida Andrea; Soñez, Carlos Alberto; Monedero Cobeta, I.; Rolando, Alicia Nelida; Romanini, María Cristina; Cots, Debora Soledad; Lazarte, M. A.; Gauna, Hector Fernando; Mugnaini, M
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The model of chronic intermittent stress by immobilization during pregnancy may produce alterations in the mechanisms that maintain adrenal gland homeostasis. In earlier investigations using this model, signifi cant variations in plasma prolactin and corticosterone levels, and adrenal gland weights were observed. We hypothesized that chronic stress causes changes in apoptosis in the adrenal glands of pregnant rats. We identifi ed and quantifi ed apoptotic cells in the adrenal cortex and examined their ultrastructural characteristics using transmission electron microscopy. Adrenal glands of pregnant rats at gestation days 12, 17 and 21 were studied for control and experimental (stressed) rats. Immunolabelling techniques, stereological analysis and image quantifi cation of adrenal gland sections were combined to determine differences in apoptosis in the different cell populations of the adrenal cortex. The apoptotic index of the experimental rats showed a signifi cant reduction at gestation day 17, while at days 12 and 21 there were no differences from controls. Moreover, the apoptotic index of the reticular zones in control and experimental animals showed a signifi cant increase compared to the glomerular and fascicular zones at the three gestation times studied. Chronic stress by immobilization reduced the caspase-dependent apoptotic index at gestation day 17, which may be related to variations in plasma concentrations of estrogens and prolactin.
Fil: Bozzo, Aida Andrea. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; Argentina
Fil: Soñez, Carlos Alberto. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; Argentina
Fil: Monedero Cobeta, I.. Universidad Autónoma de Madrid; España
Fil: Rolando, Alicia Nelida. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Romanini, María Cristina. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; Argentina
Fil: Cots, Debora Soledad. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lazarte, M. A.. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Gauna, Hector Fernando. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Mugnaini, M. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; Argentina
Materia
Adrenal Cortex
Apoptosis
Caspase 3
Chronic Stress
Electron Microscopy
Gestation
Rats
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/24079
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/24079
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Chronic stress and its effects on adrenal cortex apoptosis in pregnant ratsBozzo, Aida AndreaSoñez, Carlos AlbertoMonedero Cobeta, I.Rolando, Alicia NelidaRomanini, María CristinaCots, Debora SoledadLazarte, M. A.Gauna, Hector FernandoMugnaini, MAdrenal CortexApoptosisCaspase 3Chronic StressElectron MicroscopyGestationRatshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The model of chronic intermittent stress by immobilization during pregnancy may produce alterations in the mechanisms that maintain adrenal gland homeostasis. In earlier investigations using this model, signifi cant variations in plasma prolactin and corticosterone levels, and adrenal gland weights were observed. We hypothesized that chronic stress causes changes in apoptosis in the adrenal glands of pregnant rats. We identifi ed and quantifi ed apoptotic cells in the adrenal cortex and examined their ultrastructural characteristics using transmission electron microscopy. Adrenal glands of pregnant rats at gestation days 12, 17 and 21 were studied for control and experimental (stressed) rats. Immunolabelling techniques, stereological analysis and image quantifi cation of adrenal gland sections were combined to determine differences in apoptosis in the different cell populations of the adrenal cortex. The apoptotic index of the experimental rats showed a signifi cant reduction at gestation day 17, while at days 12 and 21 there were no differences from controls. Moreover, the apoptotic index of the reticular zones in control and experimental animals showed a signifi cant increase compared to the glomerular and fascicular zones at the three gestation times studied. Chronic stress by immobilization reduced the caspase-dependent apoptotic index at gestation day 17, which may be related to variations in plasma concentrations of estrogens and prolactin.Fil: Bozzo, Aida Andrea. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; ArgentinaFil: Soñez, Carlos Alberto. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; ArgentinaFil: Monedero Cobeta, I.. Universidad Autónoma de Madrid; EspañaFil: Rolando, Alicia Nelida. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Romanini, María Cristina. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; ArgentinaFil: Cots, Debora Soledad. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lazarte, M. A.. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; ArgentinaFil: Gauna, Hector Fernando. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaFil: Mugnaini, M. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; ArgentinaTaylor & Francis2013-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24079Bozzo, Aida Andrea; Soñez, Carlos Alberto; Monedero Cobeta, I.; Rolando, Alicia Nelida; Romanini, María Cristina; et al.; Chronic stress and its effects on adrenal cortex apoptosis in pregnant rats ; Taylor & Francis; Biotechnic And Histochemistry; 89; 4; 11-2013; 296-3031052-0295CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3109/10520295.2013.846478info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/full/10.3109/10520295.2013.846478info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:59:07Zoai:ri.conicet.gov.ar:11336/24079instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:59:07.304CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Chronic stress and its effects on adrenal cortex apoptosis in pregnant rats
title Chronic stress and its effects on adrenal cortex apoptosis in pregnant rats
spellingShingle Chronic stress and its effects on adrenal cortex apoptosis in pregnant rats
Bozzo, Aida Andrea
Adrenal Cortex
Apoptosis
Caspase 3
Chronic Stress
Electron Microscopy
Gestation
Rats
title_short Chronic stress and its effects on adrenal cortex apoptosis in pregnant rats
title_full Chronic stress and its effects on adrenal cortex apoptosis in pregnant rats
title_fullStr Chronic stress and its effects on adrenal cortex apoptosis in pregnant rats
title_full_unstemmed Chronic stress and its effects on adrenal cortex apoptosis in pregnant rats
title_sort Chronic stress and its effects on adrenal cortex apoptosis in pregnant rats
dc.creator.none.fl_str_mv Bozzo, Aida Andrea
Soñez, Carlos Alberto
Monedero Cobeta, I.
Rolando, Alicia Nelida
Romanini, María Cristina
Cots, Debora Soledad
Lazarte, M. A.
Gauna, Hector Fernando
Mugnaini, M
author Bozzo, Aida Andrea
author_facet Bozzo, Aida Andrea
Soñez, Carlos Alberto
Monedero Cobeta, I.
Rolando, Alicia Nelida
Romanini, María Cristina
Cots, Debora Soledad
Lazarte, M. A.
Gauna, Hector Fernando
Mugnaini, M
author_role author
author2 Soñez, Carlos Alberto
Monedero Cobeta, I.
Rolando, Alicia Nelida
Romanini, María Cristina
Cots, Debora Soledad
Lazarte, M. A.
Gauna, Hector Fernando
Mugnaini, M
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Adrenal Cortex
Apoptosis
Caspase 3
Chronic Stress
Electron Microscopy
Gestation
Rats
topic Adrenal Cortex
Apoptosis
Caspase 3
Chronic Stress
Electron Microscopy
Gestation
Rats
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The model of chronic intermittent stress by immobilization during pregnancy may produce alterations in the mechanisms that maintain adrenal gland homeostasis. In earlier investigations using this model, signifi cant variations in plasma prolactin and corticosterone levels, and adrenal gland weights were observed. We hypothesized that chronic stress causes changes in apoptosis in the adrenal glands of pregnant rats. We identifi ed and quantifi ed apoptotic cells in the adrenal cortex and examined their ultrastructural characteristics using transmission electron microscopy. Adrenal glands of pregnant rats at gestation days 12, 17 and 21 were studied for control and experimental (stressed) rats. Immunolabelling techniques, stereological analysis and image quantifi cation of adrenal gland sections were combined to determine differences in apoptosis in the different cell populations of the adrenal cortex. The apoptotic index of the experimental rats showed a signifi cant reduction at gestation day 17, while at days 12 and 21 there were no differences from controls. Moreover, the apoptotic index of the reticular zones in control and experimental animals showed a signifi cant increase compared to the glomerular and fascicular zones at the three gestation times studied. Chronic stress by immobilization reduced the caspase-dependent apoptotic index at gestation day 17, which may be related to variations in plasma concentrations of estrogens and prolactin.
Fil: Bozzo, Aida Andrea. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; Argentina
Fil: Soñez, Carlos Alberto. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; Argentina
Fil: Monedero Cobeta, I.. Universidad Autónoma de Madrid; España
Fil: Rolando, Alicia Nelida. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Romanini, María Cristina. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; Argentina
Fil: Cots, Debora Soledad. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lazarte, M. A.. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Gauna, Hector Fernando. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Mugnaini, M. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; Argentina
description The model of chronic intermittent stress by immobilization during pregnancy may produce alterations in the mechanisms that maintain adrenal gland homeostasis. In earlier investigations using this model, signifi cant variations in plasma prolactin and corticosterone levels, and adrenal gland weights were observed. We hypothesized that chronic stress causes changes in apoptosis in the adrenal glands of pregnant rats. We identifi ed and quantifi ed apoptotic cells in the adrenal cortex and examined their ultrastructural characteristics using transmission electron microscopy. Adrenal glands of pregnant rats at gestation days 12, 17 and 21 were studied for control and experimental (stressed) rats. Immunolabelling techniques, stereological analysis and image quantifi cation of adrenal gland sections were combined to determine differences in apoptosis in the different cell populations of the adrenal cortex. The apoptotic index of the experimental rats showed a signifi cant reduction at gestation day 17, while at days 12 and 21 there were no differences from controls. Moreover, the apoptotic index of the reticular zones in control and experimental animals showed a signifi cant increase compared to the glomerular and fascicular zones at the three gestation times studied. Chronic stress by immobilization reduced the caspase-dependent apoptotic index at gestation day 17, which may be related to variations in plasma concentrations of estrogens and prolactin.
publishDate 2013
dc.date.none.fl_str_mv 2013-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/24079
Bozzo, Aida Andrea; Soñez, Carlos Alberto; Monedero Cobeta, I.; Rolando, Alicia Nelida; Romanini, María Cristina; et al.; Chronic stress and its effects on adrenal cortex apoptosis in pregnant rats ; Taylor & Francis; Biotechnic And Histochemistry; 89; 4; 11-2013; 296-303
1052-0295
CONICET Digital
CONICET
url http://hdl.handle.net/11336/24079
identifier_str_mv Bozzo, Aida Andrea; Soñez, Carlos Alberto; Monedero Cobeta, I.; Rolando, Alicia Nelida; Romanini, María Cristina; et al.; Chronic stress and its effects on adrenal cortex apoptosis in pregnant rats ; Taylor & Francis; Biotechnic And Histochemistry; 89; 4; 11-2013; 296-303
1052-0295
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3109/10520295.2013.846478
info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/full/10.3109/10520295.2013.846478
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.891075

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