Effect of melatonin administration on the male viscacha adrenal cortex proliferative activity

Autores
Gallol, Luis Ezequiel; Busolini, Fabricio Iván; Mohamed, Fabian Heber
Año de publicación
2018
Idioma
español castellano
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The adrenal cortex, under control of the hypothalamic-pituitary-adrenal axis, produces specific steroid hormones which are key to ensure physiological adaptation and improvement of survival chances when facing homeostatic challenges. The viscacha (Lagostomus maximus maximus) is a hystricomorph rodent that lives in burrows and has nocturnal habits. The adult male viscacha exhibits an annual reproductive cycle synchronized by the environmental photoperiod through the pineal gland and its main hormone, melatonin. The aim of this study was to assess the effect of melatonin administration on the adrenocortical cells proliferative activity in adult male viscachas. For this study, animals were divided in two groups: an experimental group (n=4) that receive two daily injections of melatonin; and a control group (n=4) that receive two daily injections of the diluent. After 9 weeks, animals were sacrificed and adrenal glands processed for light microscopy. Adrenal cortex proliferation activity was assessed trough immunohistochemical detection of the proliferating cell nuclear antigen (PCNA), an S-phase related cell proliferation marker. The number of PCNA-immunoreactive adrenocortical cells (PCNA-ir) was counted per reference area. Differences between the two groups were evaluated using Mann–Whitney U test. In both experimental and control animals, cell proliferation was observed mainly in the outermost portion of the adrenal cortex, near the zona Glomerulosa and outer zona Fasciculata. In the innermost portion of the cortex PCNA-ir cells were scarce, except in some control animals where proliferation could also be observed in the inner zona Fasciculata. In melatonin administered animals, the number of PCNA-ir was (1.31  0.2) significantly lower than in control animals (2.31  0.16). These results indicate that proliferation in the adrenal cortex of the viscacha occurs mainly in the outermost portion of the cortex as described in other mammal species. Furthermore, melatonin is capable of modulating the adrenal cortex proliferative activity, reducing proliferation under stressful conditions of daily manipulation and subcutaneous injections.
Fil: Gallol, Luis Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina
Fil: Busolini, Fabricio Iván. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina
Fil: Mohamed, Fabian Heber. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina
XXXVI Reunión Científica Anual Sociedad de Biología de Cuyo
Mendoza
Argentina
Sociedad de Biología de Cuyo
Universidad Nacional de Cuyo
Materia
ADRENAL CORTEX
MELATONIN
PCNA
LAGOSTOMUS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/161395
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/161395
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network_name_str CONICET Digital (CONICET)
spelling Effect of melatonin administration on the male viscacha adrenal cortex proliferative activityGallol, Luis EzequielBusolini, Fabricio IvánMohamed, Fabian HeberADRENAL CORTEXMELATONINPCNALAGOSTOMUShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The adrenal cortex, under control of the hypothalamic-pituitary-adrenal axis, produces specific steroid hormones which are key to ensure physiological adaptation and improvement of survival chances when facing homeostatic challenges. The viscacha (Lagostomus maximus maximus) is a hystricomorph rodent that lives in burrows and has nocturnal habits. The adult male viscacha exhibits an annual reproductive cycle synchronized by the environmental photoperiod through the pineal gland and its main hormone, melatonin. The aim of this study was to assess the effect of melatonin administration on the adrenocortical cells proliferative activity in adult male viscachas. For this study, animals were divided in two groups: an experimental group (n=4) that receive two daily injections of melatonin; and a control group (n=4) that receive two daily injections of the diluent. After 9 weeks, animals were sacrificed and adrenal glands processed for light microscopy. Adrenal cortex proliferation activity was assessed trough immunohistochemical detection of the proliferating cell nuclear antigen (PCNA), an S-phase related cell proliferation marker. The number of PCNA-immunoreactive adrenocortical cells (PCNA-ir) was counted per reference area. Differences between the two groups were evaluated using Mann–Whitney U test. In both experimental and control animals, cell proliferation was observed mainly in the outermost portion of the adrenal cortex, near the zona Glomerulosa and outer zona Fasciculata. In the innermost portion of the cortex PCNA-ir cells were scarce, except in some control animals where proliferation could also be observed in the inner zona Fasciculata. In melatonin administered animals, the number of PCNA-ir was (1.31  0.2) significantly lower than in control animals (2.31  0.16). These results indicate that proliferation in the adrenal cortex of the viscacha occurs mainly in the outermost portion of the cortex as described in other mammal species. Furthermore, melatonin is capable of modulating the adrenal cortex proliferative activity, reducing proliferation under stressful conditions of daily manipulation and subcutaneous injections.Fil: Gallol, Luis Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; ArgentinaFil: Busolini, Fabricio Iván. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; ArgentinaFil: Mohamed, Fabian Heber. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; ArgentinaXXXVI Reunión Científica Anual Sociedad de Biología de CuyoMendozaArgentinaSociedad de Biología de CuyoUniversidad Nacional de CuyoUniversidad Nacional de Cuyo2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/161395Effect of melatonin administration on the male viscacha adrenal cortex proliferative activity; XXXVI Reunión Científica Anual Sociedad de Biología de Cuyo; Mendoza; Argentina; 2018; 88-88CONICET DigitalCONICETspainfo:eu-repo/semantics/altIdentifier/url/https://sbcuyo.org.ar/libro-de-resumenes-2018/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:13:59Zoai:ri.conicet.gov.ar:11336/161395instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:13:59.685CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of melatonin administration on the male viscacha adrenal cortex proliferative activity
title Effect of melatonin administration on the male viscacha adrenal cortex proliferative activity
spellingShingle Effect of melatonin administration on the male viscacha adrenal cortex proliferative activity
Gallol, Luis Ezequiel
ADRENAL CORTEX
MELATONIN
PCNA
LAGOSTOMUS
title_short Effect of melatonin administration on the male viscacha adrenal cortex proliferative activity
title_full Effect of melatonin administration on the male viscacha adrenal cortex proliferative activity
title_fullStr Effect of melatonin administration on the male viscacha adrenal cortex proliferative activity
title_full_unstemmed Effect of melatonin administration on the male viscacha adrenal cortex proliferative activity
title_sort Effect of melatonin administration on the male viscacha adrenal cortex proliferative activity
dc.creator.none.fl_str_mv Gallol, Luis Ezequiel
Busolini, Fabricio Iván
Mohamed, Fabian Heber
author Gallol, Luis Ezequiel
author_facet Gallol, Luis Ezequiel
Busolini, Fabricio Iván
Mohamed, Fabian Heber
author_role author
author2 Busolini, Fabricio Iván
Mohamed, Fabian Heber
author2_role author
author
dc.subject.none.fl_str_mv ADRENAL CORTEX
MELATONIN
PCNA
LAGOSTOMUS
topic ADRENAL CORTEX
MELATONIN
PCNA
LAGOSTOMUS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The adrenal cortex, under control of the hypothalamic-pituitary-adrenal axis, produces specific steroid hormones which are key to ensure physiological adaptation and improvement of survival chances when facing homeostatic challenges. The viscacha (Lagostomus maximus maximus) is a hystricomorph rodent that lives in burrows and has nocturnal habits. The adult male viscacha exhibits an annual reproductive cycle synchronized by the environmental photoperiod through the pineal gland and its main hormone, melatonin. The aim of this study was to assess the effect of melatonin administration on the adrenocortical cells proliferative activity in adult male viscachas. For this study, animals were divided in two groups: an experimental group (n=4) that receive two daily injections of melatonin; and a control group (n=4) that receive two daily injections of the diluent. After 9 weeks, animals were sacrificed and adrenal glands processed for light microscopy. Adrenal cortex proliferation activity was assessed trough immunohistochemical detection of the proliferating cell nuclear antigen (PCNA), an S-phase related cell proliferation marker. The number of PCNA-immunoreactive adrenocortical cells (PCNA-ir) was counted per reference area. Differences between the two groups were evaluated using Mann–Whitney U test. In both experimental and control animals, cell proliferation was observed mainly in the outermost portion of the adrenal cortex, near the zona Glomerulosa and outer zona Fasciculata. In the innermost portion of the cortex PCNA-ir cells were scarce, except in some control animals where proliferation could also be observed in the inner zona Fasciculata. In melatonin administered animals, the number of PCNA-ir was (1.31  0.2) significantly lower than in control animals (2.31  0.16). These results indicate that proliferation in the adrenal cortex of the viscacha occurs mainly in the outermost portion of the cortex as described in other mammal species. Furthermore, melatonin is capable of modulating the adrenal cortex proliferative activity, reducing proliferation under stressful conditions of daily manipulation and subcutaneous injections.
Fil: Gallol, Luis Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina
Fil: Busolini, Fabricio Iván. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina
Fil: Mohamed, Fabian Heber. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina
XXXVI Reunión Científica Anual Sociedad de Biología de Cuyo
Mendoza
Argentina
Sociedad de Biología de Cuyo
Universidad Nacional de Cuyo
description The adrenal cortex, under control of the hypothalamic-pituitary-adrenal axis, produces specific steroid hormones which are key to ensure physiological adaptation and improvement of survival chances when facing homeostatic challenges. The viscacha (Lagostomus maximus maximus) is a hystricomorph rodent that lives in burrows and has nocturnal habits. The adult male viscacha exhibits an annual reproductive cycle synchronized by the environmental photoperiod through the pineal gland and its main hormone, melatonin. The aim of this study was to assess the effect of melatonin administration on the adrenocortical cells proliferative activity in adult male viscachas. For this study, animals were divided in two groups: an experimental group (n=4) that receive two daily injections of melatonin; and a control group (n=4) that receive two daily injections of the diluent. After 9 weeks, animals were sacrificed and adrenal glands processed for light microscopy. Adrenal cortex proliferation activity was assessed trough immunohistochemical detection of the proliferating cell nuclear antigen (PCNA), an S-phase related cell proliferation marker. The number of PCNA-immunoreactive adrenocortical cells (PCNA-ir) was counted per reference area. Differences between the two groups were evaluated using Mann–Whitney U test. In both experimental and control animals, cell proliferation was observed mainly in the outermost portion of the adrenal cortex, near the zona Glomerulosa and outer zona Fasciculata. In the innermost portion of the cortex PCNA-ir cells were scarce, except in some control animals where proliferation could also be observed in the inner zona Fasciculata. In melatonin administered animals, the number of PCNA-ir was (1.31  0.2) significantly lower than in control animals (2.31  0.16). These results indicate that proliferation in the adrenal cortex of the viscacha occurs mainly in the outermost portion of the cortex as described in other mammal species. Furthermore, melatonin is capable of modulating the adrenal cortex proliferative activity, reducing proliferation under stressful conditions of daily manipulation and subcutaneous injections.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/161395
Effect of melatonin administration on the male viscacha adrenal cortex proliferative activity; XXXVI Reunión Científica Anual Sociedad de Biología de Cuyo; Mendoza; Argentina; 2018; 88-88
CONICET Digital
CONICET
url http://hdl.handle.net/11336/161395
identifier_str_mv Effect of melatonin administration on the male viscacha adrenal cortex proliferative activity; XXXVI Reunión Científica Anual Sociedad de Biología de Cuyo; Mendoza; Argentina; 2018; 88-88
CONICET Digital
CONICET
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language spa
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://sbcuyo.org.ar/libro-de-resumenes-2018/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Universidad Nacional de Cuyo
publisher.none.fl_str_mv Universidad Nacional de Cuyo
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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