Effect of melatonin administration on the male viscacha adrenal cortex proliferative activity
- Autores
- Gallol, Luis Ezequiel; Busolini, Fabricio Iván; Mohamed, Fabian Heber
- Año de publicación
- 2018
- Idioma
- español castellano
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- The adrenal cortex, under control of the hypothalamic-pituitary-adrenal axis, produces specific steroid hormones which are key to ensure physiological adaptation and improvement of survival chances when facing homeostatic challenges. The viscacha (Lagostomus maximus maximus) is a hystricomorph rodent that lives in burrows and has nocturnal habits. The adult male viscacha exhibits an annual reproductive cycle synchronized by the environmental photoperiod through the pineal gland and its main hormone, melatonin. The aim of this study was to assess the effect of melatonin administration on the adrenocortical cells proliferative activity in adult male viscachas. For this study, animals were divided in two groups: an experimental group (n=4) that receive two daily injections of melatonin; and a control group (n=4) that receive two daily injections of the diluent. After 9 weeks, animals were sacrificed and adrenal glands processed for light microscopy. Adrenal cortex proliferation activity was assessed trough immunohistochemical detection of the proliferating cell nuclear antigen (PCNA), an S-phase related cell proliferation marker. The number of PCNA-immunoreactive adrenocortical cells (PCNA-ir) was counted per reference area. Differences between the two groups were evaluated using Mann–Whitney U test. In both experimental and control animals, cell proliferation was observed mainly in the outermost portion of the adrenal cortex, near the zona Glomerulosa and outer zona Fasciculata. In the innermost portion of the cortex PCNA-ir cells were scarce, except in some control animals where proliferation could also be observed in the inner zona Fasciculata. In melatonin administered animals, the number of PCNA-ir was (1.31 0.2) significantly lower than in control animals (2.31 0.16). These results indicate that proliferation in the adrenal cortex of the viscacha occurs mainly in the outermost portion of the cortex as described in other mammal species. Furthermore, melatonin is capable of modulating the adrenal cortex proliferative activity, reducing proliferation under stressful conditions of daily manipulation and subcutaneous injections.
Fil: Gallol, Luis Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina
Fil: Busolini, Fabricio Iván. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina
Fil: Mohamed, Fabian Heber. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina
XXXVI Reunión Científica Anual Sociedad de Biología de Cuyo
Mendoza
Argentina
Sociedad de Biología de Cuyo
Universidad Nacional de Cuyo - Materia
-
ADRENAL CORTEX
MELATONIN
PCNA
LAGOSTOMUS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/161395
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Effect of melatonin administration on the male viscacha adrenal cortex proliferative activityGallol, Luis EzequielBusolini, Fabricio IvánMohamed, Fabian HeberADRENAL CORTEXMELATONINPCNALAGOSTOMUShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The adrenal cortex, under control of the hypothalamic-pituitary-adrenal axis, produces specific steroid hormones which are key to ensure physiological adaptation and improvement of survival chances when facing homeostatic challenges. The viscacha (Lagostomus maximus maximus) is a hystricomorph rodent that lives in burrows and has nocturnal habits. The adult male viscacha exhibits an annual reproductive cycle synchronized by the environmental photoperiod through the pineal gland and its main hormone, melatonin. The aim of this study was to assess the effect of melatonin administration on the adrenocortical cells proliferative activity in adult male viscachas. For this study, animals were divided in two groups: an experimental group (n=4) that receive two daily injections of melatonin; and a control group (n=4) that receive two daily injections of the diluent. After 9 weeks, animals were sacrificed and adrenal glands processed for light microscopy. Adrenal cortex proliferation activity was assessed trough immunohistochemical detection of the proliferating cell nuclear antigen (PCNA), an S-phase related cell proliferation marker. The number of PCNA-immunoreactive adrenocortical cells (PCNA-ir) was counted per reference area. Differences between the two groups were evaluated using Mann–Whitney U test. In both experimental and control animals, cell proliferation was observed mainly in the outermost portion of the adrenal cortex, near the zona Glomerulosa and outer zona Fasciculata. In the innermost portion of the cortex PCNA-ir cells were scarce, except in some control animals where proliferation could also be observed in the inner zona Fasciculata. In melatonin administered animals, the number of PCNA-ir was (1.31 0.2) significantly lower than in control animals (2.31 0.16). These results indicate that proliferation in the adrenal cortex of the viscacha occurs mainly in the outermost portion of the cortex as described in other mammal species. Furthermore, melatonin is capable of modulating the adrenal cortex proliferative activity, reducing proliferation under stressful conditions of daily manipulation and subcutaneous injections.Fil: Gallol, Luis Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; ArgentinaFil: Busolini, Fabricio Iván. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; ArgentinaFil: Mohamed, Fabian Heber. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; ArgentinaXXXVI Reunión Científica Anual Sociedad de Biología de CuyoMendozaArgentinaSociedad de Biología de CuyoUniversidad Nacional de CuyoUniversidad Nacional de Cuyo2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/161395Effect of melatonin administration on the male viscacha adrenal cortex proliferative activity; XXXVI Reunión Científica Anual Sociedad de Biología de Cuyo; Mendoza; Argentina; 2018; 88-88CONICET DigitalCONICETspainfo:eu-repo/semantics/altIdentifier/url/https://sbcuyo.org.ar/libro-de-resumenes-2018/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:45:06Zoai:ri.conicet.gov.ar:11336/161395instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:45:06.511CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Effect of melatonin administration on the male viscacha adrenal cortex proliferative activity |
title |
Effect of melatonin administration on the male viscacha adrenal cortex proliferative activity |
spellingShingle |
Effect of melatonin administration on the male viscacha adrenal cortex proliferative activity Gallol, Luis Ezequiel ADRENAL CORTEX MELATONIN PCNA LAGOSTOMUS |
title_short |
Effect of melatonin administration on the male viscacha adrenal cortex proliferative activity |
title_full |
Effect of melatonin administration on the male viscacha adrenal cortex proliferative activity |
title_fullStr |
Effect of melatonin administration on the male viscacha adrenal cortex proliferative activity |
title_full_unstemmed |
Effect of melatonin administration on the male viscacha adrenal cortex proliferative activity |
title_sort |
Effect of melatonin administration on the male viscacha adrenal cortex proliferative activity |
dc.creator.none.fl_str_mv |
Gallol, Luis Ezequiel Busolini, Fabricio Iván Mohamed, Fabian Heber |
author |
Gallol, Luis Ezequiel |
author_facet |
Gallol, Luis Ezequiel Busolini, Fabricio Iván Mohamed, Fabian Heber |
author_role |
author |
author2 |
Busolini, Fabricio Iván Mohamed, Fabian Heber |
author2_role |
author author |
dc.subject.none.fl_str_mv |
ADRENAL CORTEX MELATONIN PCNA LAGOSTOMUS |
topic |
ADRENAL CORTEX MELATONIN PCNA LAGOSTOMUS |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
The adrenal cortex, under control of the hypothalamic-pituitary-adrenal axis, produces specific steroid hormones which are key to ensure physiological adaptation and improvement of survival chances when facing homeostatic challenges. The viscacha (Lagostomus maximus maximus) is a hystricomorph rodent that lives in burrows and has nocturnal habits. The adult male viscacha exhibits an annual reproductive cycle synchronized by the environmental photoperiod through the pineal gland and its main hormone, melatonin. The aim of this study was to assess the effect of melatonin administration on the adrenocortical cells proliferative activity in adult male viscachas. For this study, animals were divided in two groups: an experimental group (n=4) that receive two daily injections of melatonin; and a control group (n=4) that receive two daily injections of the diluent. After 9 weeks, animals were sacrificed and adrenal glands processed for light microscopy. Adrenal cortex proliferation activity was assessed trough immunohistochemical detection of the proliferating cell nuclear antigen (PCNA), an S-phase related cell proliferation marker. The number of PCNA-immunoreactive adrenocortical cells (PCNA-ir) was counted per reference area. Differences between the two groups were evaluated using Mann–Whitney U test. In both experimental and control animals, cell proliferation was observed mainly in the outermost portion of the adrenal cortex, near the zona Glomerulosa and outer zona Fasciculata. In the innermost portion of the cortex PCNA-ir cells were scarce, except in some control animals where proliferation could also be observed in the inner zona Fasciculata. In melatonin administered animals, the number of PCNA-ir was (1.31 0.2) significantly lower than in control animals (2.31 0.16). These results indicate that proliferation in the adrenal cortex of the viscacha occurs mainly in the outermost portion of the cortex as described in other mammal species. Furthermore, melatonin is capable of modulating the adrenal cortex proliferative activity, reducing proliferation under stressful conditions of daily manipulation and subcutaneous injections. Fil: Gallol, Luis Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina Fil: Busolini, Fabricio Iván. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina Fil: Mohamed, Fabian Heber. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina XXXVI Reunión Científica Anual Sociedad de Biología de Cuyo Mendoza Argentina Sociedad de Biología de Cuyo Universidad Nacional de Cuyo |
description |
The adrenal cortex, under control of the hypothalamic-pituitary-adrenal axis, produces specific steroid hormones which are key to ensure physiological adaptation and improvement of survival chances when facing homeostatic challenges. The viscacha (Lagostomus maximus maximus) is a hystricomorph rodent that lives in burrows and has nocturnal habits. The adult male viscacha exhibits an annual reproductive cycle synchronized by the environmental photoperiod through the pineal gland and its main hormone, melatonin. The aim of this study was to assess the effect of melatonin administration on the adrenocortical cells proliferative activity in adult male viscachas. For this study, animals were divided in two groups: an experimental group (n=4) that receive two daily injections of melatonin; and a control group (n=4) that receive two daily injections of the diluent. After 9 weeks, animals were sacrificed and adrenal glands processed for light microscopy. Adrenal cortex proliferation activity was assessed trough immunohistochemical detection of the proliferating cell nuclear antigen (PCNA), an S-phase related cell proliferation marker. The number of PCNA-immunoreactive adrenocortical cells (PCNA-ir) was counted per reference area. Differences between the two groups were evaluated using Mann–Whitney U test. In both experimental and control animals, cell proliferation was observed mainly in the outermost portion of the adrenal cortex, near the zona Glomerulosa and outer zona Fasciculata. In the innermost portion of the cortex PCNA-ir cells were scarce, except in some control animals where proliferation could also be observed in the inner zona Fasciculata. In melatonin administered animals, the number of PCNA-ir was (1.31 0.2) significantly lower than in control animals (2.31 0.16). These results indicate that proliferation in the adrenal cortex of the viscacha occurs mainly in the outermost portion of the cortex as described in other mammal species. Furthermore, melatonin is capable of modulating the adrenal cortex proliferative activity, reducing proliferation under stressful conditions of daily manipulation and subcutaneous injections. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Reunión Book http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
status_str |
publishedVersion |
format |
conferenceObject |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/161395 Effect of melatonin administration on the male viscacha adrenal cortex proliferative activity; XXXVI Reunión Científica Anual Sociedad de Biología de Cuyo; Mendoza; Argentina; 2018; 88-88 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/161395 |
identifier_str_mv |
Effect of melatonin administration on the male viscacha adrenal cortex proliferative activity; XXXVI Reunión Científica Anual Sociedad de Biología de Cuyo; Mendoza; Argentina; 2018; 88-88 CONICET Digital CONICET |
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info:eu-repo/semantics/altIdentifier/url/https://sbcuyo.org.ar/libro-de-resumenes-2018/ |
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https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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Universidad Nacional de Cuyo |
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Universidad Nacional de Cuyo |
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