Adrenal gland responses to lipopolysaccharide after stress and ethanol administration in male rats

Autores
Mohn, Claudia Ester; Fernández Solari, Jose Javier; de Laurentiis, Andrea; Bornstein, S. R.; Ehrhat Bornstein, M.; Besuhli, Valeria
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
All forms of stress, including restraint stress (RS) and lipopolysaccharide (LPS) administration, activate the hypothalamic–pituitary–adrenal (HPA) axis. LPS binds to a recognition protein (CD14) and toll-like receptor 2/4 in different cells and tissues, including the adrenal gland, to induce the production of cytokines and cause upregulation of cyclooxygenase and nitric oxide synthase (NOS) enzymes. Acute ethanol exposure activates the HPA axis, but in some conditions prolonged administration can dampen this activation as well as decrease the inflammatory responses to LPS. Therefore, this study was designed to evaluate the adrenal response to a challenge dose of LPS (50 mg/kg) injected i.p., after submitting male rats to RS, twice a day (2 h each time) for 5 days and/or ethanol administration (3 g/kg) by gavage also for 5 days, twice daily. At the end of the experiment, plasma corticosterone concentrations and adrenal gland content of prostaglandin E (PGE) and NOS activity were measured as stress mediators. The results showed that repetitive ethanol administration attenuated the adrenal stress response to LPS challenge alone and after RS, by preventing the increase in plasma corticosterone concentrations and by decreasing the PGE content and NOS activity in the adrenal gland. Therefore, we conclude that moderate alcohol consumption could attenuate the effects of psychophysical stress and impair an inflammatory response.
Fil: Mohn, Claudia Ester. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Fernández Solari, Jose Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: de Laurentiis, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Bornstein, S. R.. Carl Gustav Carus University Hospital; Alemania
Fil: Ehrhat Bornstein, M.. Carl Gustav Carus University Hospital; Alemania
Fil: Besuhli, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina
Materia
Adrenal Cortex
Corticosterone
Endotoxin
Nitric Oxide
Prostaglandin
Restrain Stress
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/12918
Acceder
id CONICETDig_49dd21322041a7ea895d7a674f0ecbdb
oai_identifier_str oai:ri.conicet.gov.ar:11336/12918
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Adrenal gland responses to lipopolysaccharide after stress and ethanol administration in male ratsMohn, Claudia EsterFernández Solari, Jose Javierde Laurentiis, AndreaBornstein, S. R.Ehrhat Bornstein, M.Besuhli, ValeriaAdrenal CortexCorticosteroneEndotoxinNitric OxideProstaglandinRestrain Stresshttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3All forms of stress, including restraint stress (RS) and lipopolysaccharide (LPS) administration, activate the hypothalamic–pituitary–adrenal (HPA) axis. LPS binds to a recognition protein (CD14) and toll-like receptor 2/4 in different cells and tissues, including the adrenal gland, to induce the production of cytokines and cause upregulation of cyclooxygenase and nitric oxide synthase (NOS) enzymes. Acute ethanol exposure activates the HPA axis, but in some conditions prolonged administration can dampen this activation as well as decrease the inflammatory responses to LPS. Therefore, this study was designed to evaluate the adrenal response to a challenge dose of LPS (50 mg/kg) injected i.p., after submitting male rats to RS, twice a day (2 h each time) for 5 days and/or ethanol administration (3 g/kg) by gavage also for 5 days, twice daily. At the end of the experiment, plasma corticosterone concentrations and adrenal gland content of prostaglandin E (PGE) and NOS activity were measured as stress mediators. The results showed that repetitive ethanol administration attenuated the adrenal stress response to LPS challenge alone and after RS, by preventing the increase in plasma corticosterone concentrations and by decreasing the PGE content and NOS activity in the adrenal gland. Therefore, we conclude that moderate alcohol consumption could attenuate the effects of psychophysical stress and impair an inflammatory response.Fil: Mohn, Claudia Ester. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Fernández Solari, Jose Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: de Laurentiis, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Bornstein, S. R.. Carl Gustav Carus University Hospital; AlemaniaFil: Ehrhat Bornstein, M.. Carl Gustav Carus University Hospital; AlemaniaFil: Besuhli, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; ArgentinaTaylor & Francis Ltd2011-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/12918Mohn, Claudia Ester; Fernández Solari, Jose Javier; de Laurentiis, Andrea; Bornstein, S. R.; Ehrhat Bornstein, M.; et al.; Adrenal gland responses to lipopolysaccharide after stress and ethanol administration in male rats; Taylor & Francis Ltd; Stress; 14; 2; 3-2011; 216-2261025-3890enginfo:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/abs/10.3109/10253890.2010.532254?journalCode=ists20info:eu-repo/semantics/altIdentifier/doi/10.3109/10253890.2010.532254info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:36:37Zoai:ri.conicet.gov.ar:11336/12918instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:36:37.404CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Adrenal gland responses to lipopolysaccharide after stress and ethanol administration in male rats
title Adrenal gland responses to lipopolysaccharide after stress and ethanol administration in male rats
spellingShingle Adrenal gland responses to lipopolysaccharide after stress and ethanol administration in male rats
Mohn, Claudia Ester
Adrenal Cortex
Corticosterone
Endotoxin
Nitric Oxide
Prostaglandin
Restrain Stress
title_short Adrenal gland responses to lipopolysaccharide after stress and ethanol administration in male rats
title_full Adrenal gland responses to lipopolysaccharide after stress and ethanol administration in male rats
title_fullStr Adrenal gland responses to lipopolysaccharide after stress and ethanol administration in male rats
title_full_unstemmed Adrenal gland responses to lipopolysaccharide after stress and ethanol administration in male rats
title_sort Adrenal gland responses to lipopolysaccharide after stress and ethanol administration in male rats
dc.creator.none.fl_str_mv Mohn, Claudia Ester
Fernández Solari, Jose Javier
de Laurentiis, Andrea
Bornstein, S. R.
Ehrhat Bornstein, M.
Besuhli, Valeria
author Mohn, Claudia Ester
author_facet Mohn, Claudia Ester
Fernández Solari, Jose Javier
de Laurentiis, Andrea
Bornstein, S. R.
Ehrhat Bornstein, M.
Besuhli, Valeria
author_role author
author2 Fernández Solari, Jose Javier
de Laurentiis, Andrea
Bornstein, S. R.
Ehrhat Bornstein, M.
Besuhli, Valeria
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Adrenal Cortex
Corticosterone
Endotoxin
Nitric Oxide
Prostaglandin
Restrain Stress
topic Adrenal Cortex
Corticosterone
Endotoxin
Nitric Oxide
Prostaglandin
Restrain Stress
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv All forms of stress, including restraint stress (RS) and lipopolysaccharide (LPS) administration, activate the hypothalamic–pituitary–adrenal (HPA) axis. LPS binds to a recognition protein (CD14) and toll-like receptor 2/4 in different cells and tissues, including the adrenal gland, to induce the production of cytokines and cause upregulation of cyclooxygenase and nitric oxide synthase (NOS) enzymes. Acute ethanol exposure activates the HPA axis, but in some conditions prolonged administration can dampen this activation as well as decrease the inflammatory responses to LPS. Therefore, this study was designed to evaluate the adrenal response to a challenge dose of LPS (50 mg/kg) injected i.p., after submitting male rats to RS, twice a day (2 h each time) for 5 days and/or ethanol administration (3 g/kg) by gavage also for 5 days, twice daily. At the end of the experiment, plasma corticosterone concentrations and adrenal gland content of prostaglandin E (PGE) and NOS activity were measured as stress mediators. The results showed that repetitive ethanol administration attenuated the adrenal stress response to LPS challenge alone and after RS, by preventing the increase in plasma corticosterone concentrations and by decreasing the PGE content and NOS activity in the adrenal gland. Therefore, we conclude that moderate alcohol consumption could attenuate the effects of psychophysical stress and impair an inflammatory response.
Fil: Mohn, Claudia Ester. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Fernández Solari, Jose Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: de Laurentiis, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Bornstein, S. R.. Carl Gustav Carus University Hospital; Alemania
Fil: Ehrhat Bornstein, M.. Carl Gustav Carus University Hospital; Alemania
Fil: Besuhli, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina
description All forms of stress, including restraint stress (RS) and lipopolysaccharide (LPS) administration, activate the hypothalamic–pituitary–adrenal (HPA) axis. LPS binds to a recognition protein (CD14) and toll-like receptor 2/4 in different cells and tissues, including the adrenal gland, to induce the production of cytokines and cause upregulation of cyclooxygenase and nitric oxide synthase (NOS) enzymes. Acute ethanol exposure activates the HPA axis, but in some conditions prolonged administration can dampen this activation as well as decrease the inflammatory responses to LPS. Therefore, this study was designed to evaluate the adrenal response to a challenge dose of LPS (50 mg/kg) injected i.p., after submitting male rats to RS, twice a day (2 h each time) for 5 days and/or ethanol administration (3 g/kg) by gavage also for 5 days, twice daily. At the end of the experiment, plasma corticosterone concentrations and adrenal gland content of prostaglandin E (PGE) and NOS activity were measured as stress mediators. The results showed that repetitive ethanol administration attenuated the adrenal stress response to LPS challenge alone and after RS, by preventing the increase in plasma corticosterone concentrations and by decreasing the PGE content and NOS activity in the adrenal gland. Therefore, we conclude that moderate alcohol consumption could attenuate the effects of psychophysical stress and impair an inflammatory response.
publishDate 2011
dc.date.none.fl_str_mv 2011-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/12918
Mohn, Claudia Ester; Fernández Solari, Jose Javier; de Laurentiis, Andrea; Bornstein, S. R.; Ehrhat Bornstein, M.; et al.; Adrenal gland responses to lipopolysaccharide after stress and ethanol administration in male rats; Taylor & Francis Ltd; Stress; 14; 2; 3-2011; 216-226
1025-3890
url http://hdl.handle.net/11336/12918
identifier_str_mv Mohn, Claudia Ester; Fernández Solari, Jose Javier; de Laurentiis, Andrea; Bornstein, S. R.; Ehrhat Bornstein, M.; et al.; Adrenal gland responses to lipopolysaccharide after stress and ethanol administration in male rats; Taylor & Francis Ltd; Stress; 14; 2; 3-2011; 216-226
1025-3890
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/abs/10.3109/10253890.2010.532254?journalCode=ists20
info:eu-repo/semantics/altIdentifier/doi/10.3109/10253890.2010.532254
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis Ltd
publisher.none.fl_str_mv Taylor & Francis Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083488822853632
score 12.891075

Publicaciones similares