Statistical evaluation of medium components of entomopathogenic protein production

Autores
Loto, Flavia del Valle; Carrizo, Alfonso Emanuel; Baigori, Mario Domingo; Pera, Licia Maria
Año de publicación
2010
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Bacillus thuringiensis produces proteins with entomopathogenic action. Advances in the production of bio-insecticides involve the application of suitable fermentation technologies, especially with the use of appropriate media, overcoming the metabolic limitations of the microorganisms. The use of agro-industrial residues as substrate in the production by fermentation can significantly reduce the final price and add value to low-cost materials. The objective of the present work was to screening substrates that have a positive impact on the production of entomopathogenic proteins from Bacillus thuringiensis RT. Evaluation was performed by Plackett-Burman experimental design. Eleven medium components were evaluated. Mortality bioassays were carried out using 3rd instar larvae of Spodoptera frugiperda. The most significant variables affecting positively the production were starch, milk powder and whey. Medium with highest protein production (0,8 mg/ml) was 4 times better than Luria Bertani medium (0,2mg/ml). There were significant differences in mortality with the media tested. In conclusion, many substrates were identified to improve production, which could be considered for optimization medium and studies inbioreactors.This work was supported by grants PIP-CONICET 297 and CIUNT 26/D409.
Fil: Loto, Flavia del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Carrizo, Alfonso Emanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Baigori, Mario Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Pera, Licia Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
XLVI Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Puerto Madryn
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Materia
BACILLUS THURINGIENSIS
SPODOPTERA FRUGIPERDA
ENTOMOPATHOGENIC
PLACKETT-BURMAN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/194954
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/194954
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Statistical evaluation of medium components of entomopathogenic protein productionLoto, Flavia del ValleCarrizo, Alfonso EmanuelBaigori, Mario DomingoPera, Licia MariaBACILLUS THURINGIENSISSPODOPTERA FRUGIPERDAENTOMOPATHOGENICPLACKETT-BURMANhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Bacillus thuringiensis produces proteins with entomopathogenic action. Advances in the production of bio-insecticides involve the application of suitable fermentation technologies, especially with the use of appropriate media, overcoming the metabolic limitations of the microorganisms. The use of agro-industrial residues as substrate in the production by fermentation can significantly reduce the final price and add value to low-cost materials. The objective of the present work was to screening substrates that have a positive impact on the production of entomopathogenic proteins from Bacillus thuringiensis RT. Evaluation was performed by Plackett-Burman experimental design. Eleven medium components were evaluated. Mortality bioassays were carried out using 3rd instar larvae of Spodoptera frugiperda. The most significant variables affecting positively the production were starch, milk powder and whey. Medium with highest protein production (0,8 mg/ml) was 4 times better than Luria Bertani medium (0,2mg/ml). There were significant differences in mortality with the media tested. In conclusion, many substrates were identified to improve production, which could be considered for optimization medium and studies inbioreactors.This work was supported by grants PIP-CONICET 297 and CIUNT 26/D409.Fil: Loto, Flavia del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Carrizo, Alfonso Emanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Baigori, Mario Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Pera, Licia Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaXLVI Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología MolecularPuerto MadrynArgentinaSociedad Argentina de Investigación en Bioquímica y Biología MolecularSociedad Argentina de Investigación en Bioquímica y Biología Molecular2010info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/194954Statistical evaluation of medium components of entomopathogenic protein production; XLVI Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Puerto Madryn; Argentina; 2010; 115-1151667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://saib.org.ar/archivos/biocell-34.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:33:31Zoai:ri.conicet.gov.ar:11336/194954instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:33:31.362CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Statistical evaluation of medium components of entomopathogenic protein production
title Statistical evaluation of medium components of entomopathogenic protein production
spellingShingle Statistical evaluation of medium components of entomopathogenic protein production
Loto, Flavia del Valle
BACILLUS THURINGIENSIS
SPODOPTERA FRUGIPERDA
ENTOMOPATHOGENIC
PLACKETT-BURMAN
title_short Statistical evaluation of medium components of entomopathogenic protein production
title_full Statistical evaluation of medium components of entomopathogenic protein production
title_fullStr Statistical evaluation of medium components of entomopathogenic protein production
title_full_unstemmed Statistical evaluation of medium components of entomopathogenic protein production
title_sort Statistical evaluation of medium components of entomopathogenic protein production
dc.creator.none.fl_str_mv Loto, Flavia del Valle
Carrizo, Alfonso Emanuel
Baigori, Mario Domingo
Pera, Licia Maria
author Loto, Flavia del Valle
author_facet Loto, Flavia del Valle
Carrizo, Alfonso Emanuel
Baigori, Mario Domingo
Pera, Licia Maria
author_role author
author2 Carrizo, Alfonso Emanuel
Baigori, Mario Domingo
Pera, Licia Maria
author2_role author
author
author
dc.subject.none.fl_str_mv BACILLUS THURINGIENSIS
SPODOPTERA FRUGIPERDA
ENTOMOPATHOGENIC
PLACKETT-BURMAN
topic BACILLUS THURINGIENSIS
SPODOPTERA FRUGIPERDA
ENTOMOPATHOGENIC
PLACKETT-BURMAN
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Bacillus thuringiensis produces proteins with entomopathogenic action. Advances in the production of bio-insecticides involve the application of suitable fermentation technologies, especially with the use of appropriate media, overcoming the metabolic limitations of the microorganisms. The use of agro-industrial residues as substrate in the production by fermentation can significantly reduce the final price and add value to low-cost materials. The objective of the present work was to screening substrates that have a positive impact on the production of entomopathogenic proteins from Bacillus thuringiensis RT. Evaluation was performed by Plackett-Burman experimental design. Eleven medium components were evaluated. Mortality bioassays were carried out using 3rd instar larvae of Spodoptera frugiperda. The most significant variables affecting positively the production were starch, milk powder and whey. Medium with highest protein production (0,8 mg/ml) was 4 times better than Luria Bertani medium (0,2mg/ml). There were significant differences in mortality with the media tested. In conclusion, many substrates were identified to improve production, which could be considered for optimization medium and studies inbioreactors.This work was supported by grants PIP-CONICET 297 and CIUNT 26/D409.
Fil: Loto, Flavia del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Carrizo, Alfonso Emanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Baigori, Mario Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Pera, Licia Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
XLVI Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Puerto Madryn
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
description Bacillus thuringiensis produces proteins with entomopathogenic action. Advances in the production of bio-insecticides involve the application of suitable fermentation technologies, especially with the use of appropriate media, overcoming the metabolic limitations of the microorganisms. The use of agro-industrial residues as substrate in the production by fermentation can significantly reduce the final price and add value to low-cost materials. The objective of the present work was to screening substrates that have a positive impact on the production of entomopathogenic proteins from Bacillus thuringiensis RT. Evaluation was performed by Plackett-Burman experimental design. Eleven medium components were evaluated. Mortality bioassays were carried out using 3rd instar larvae of Spodoptera frugiperda. The most significant variables affecting positively the production were starch, milk powder and whey. Medium with highest protein production (0,8 mg/ml) was 4 times better than Luria Bertani medium (0,2mg/ml). There were significant differences in mortality with the media tested. In conclusion, many substrates were identified to improve production, which could be considered for optimization medium and studies inbioreactors.This work was supported by grants PIP-CONICET 297 and CIUNT 26/D409.
publishDate 2010
dc.date.none.fl_str_mv 2010
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/194954
Statistical evaluation of medium components of entomopathogenic protein production; XLVI Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Puerto Madryn; Argentina; 2010; 115-115
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/194954
identifier_str_mv Statistical evaluation of medium components of entomopathogenic protein production; XLVI Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Puerto Madryn; Argentina; 2010; 115-115
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://saib.org.ar/archivos/biocell-34.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
publisher.none.fl_str_mv Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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