Chylomicron Characteristics Are Associated With Microsomal Triglyceride Transfer Protein in an Animal Model of Diet-Induced Dysbiosis

Autores
Olano, Carolina; Fariña, Gregorio; Wiszniewski, Morena; Medel, Jimena; Morales, Celina; Friedman, Silvia María; Macri, Elisa Vanesa; Barchuk, Magalí; Berg, Gabriela Alicia; Schreier, Laura Ester; Zago, Valeria
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objective: Intestinal remnant chylomicrons (CMs) are involved in cardiovascular residual risk and the atherogenic process. Microsomal triglyceride transfer protein (MTTP) catalyzes the assembly of lipids to apolipoprotein B48, generating CMs. Dysbiosis could alter this behavior. This study investigated the chemical composition of CMs and their associations with intestinal MTTP and gut fat depots in a diet-induced dysbiosis animal model.MethodsMale Wistar rats were fed either a standard diet (control, n=10) or a high-fat high-sucrose diet (HFSD, n=10) for 14 weeks. Measurements included serum glucose, lipid-lipoprotein profile, free fatty acids (FFAs), lipopolysaccharide (LPS) and the Firmicutes/Bacteroidetes (F/B) ratio in stool samples, via real-time quantitative polymerase chain reaction. Lipid content in isolated CMs (ultracentrifugation d <0.95 g/mL) was assessed, and MTTP, cell intestinal fat content (CIF), histology, apoB mRNA and tight junction (TJ) proteins were analyzed, in intestinal tissue.ResultsCompared to control, HFSD rats showed higher levels of LPS, triglycerides (TGs), non-high-density lipoprotein cholesterol (HDL-C) levels, TG/HDL-C ratio, FFAs, and the F/B ratio. HFSD CMs showed increased TG and phospholipids. TJ proteins levels were lower in the HFSD group, while histological scores showed no differences. CIF was increased in the HFSD group. No significant differences in apoB mRNA were found. MTTP expression was higher in the HFSD group, and directly correlated with CM-TG and inversely correlated with CIF.ConclusionOur findings imply that gut TG content may constitute an important determinant of the secretion of TG-rich CMs, promoted by MTTP, with increased atherogenic potential.
Fil: Olano, Carolina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
Fil: Fariña, Gregorio. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Wiszniewski, Morena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Medel, Jimena. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiopatología Cardiovascular; Argentina
Fil: Morales, Celina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiopatología Cardiovascular; Argentina
Fil: Friedman, Silvia María. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Bioquímica General y Bucal; Argentina
Fil: Macri, Elisa Vanesa. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Bioquímica General y Bucal; Argentina
Fil: Barchuk, Magalí. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Berg, Gabriela Alicia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Schreier, Laura Ester. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
Fil: Zago, Valeria. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Chylomicrons
Dysbiosis
Triglycerides
Atherogenic risk
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/272439
Acceder
id CONICETDig_8c7888dd3df87426b8cb907ab3386c58
oai_identifier_str oai:ri.conicet.gov.ar:11336/272439
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Chylomicron Characteristics Are Associated With Microsomal Triglyceride Transfer Protein in an Animal Model of Diet-Induced DysbiosisOlano, CarolinaFariña, GregorioWiszniewski, MorenaMedel, JimenaMorales, CelinaFriedman, Silvia MaríaMacri, Elisa VanesaBarchuk, MagalíBerg, Gabriela AliciaSchreier, Laura EsterZago, ValeriaChylomicronsDysbiosisTriglyceridesAtherogenic riskhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Objective: Intestinal remnant chylomicrons (CMs) are involved in cardiovascular residual risk and the atherogenic process. Microsomal triglyceride transfer protein (MTTP) catalyzes the assembly of lipids to apolipoprotein B48, generating CMs. Dysbiosis could alter this behavior. This study investigated the chemical composition of CMs and their associations with intestinal MTTP and gut fat depots in a diet-induced dysbiosis animal model.MethodsMale Wistar rats were fed either a standard diet (control, n=10) or a high-fat high-sucrose diet (HFSD, n=10) for 14 weeks. Measurements included serum glucose, lipid-lipoprotein profile, free fatty acids (FFAs), lipopolysaccharide (LPS) and the Firmicutes/Bacteroidetes (F/B) ratio in stool samples, via real-time quantitative polymerase chain reaction. Lipid content in isolated CMs (ultracentrifugation d <0.95 g/mL) was assessed, and MTTP, cell intestinal fat content (CIF), histology, apoB mRNA and tight junction (TJ) proteins were analyzed, in intestinal tissue.ResultsCompared to control, HFSD rats showed higher levels of LPS, triglycerides (TGs), non-high-density lipoprotein cholesterol (HDL-C) levels, TG/HDL-C ratio, FFAs, and the F/B ratio. HFSD CMs showed increased TG and phospholipids. TJ proteins levels were lower in the HFSD group, while histological scores showed no differences. CIF was increased in the HFSD group. No significant differences in apoB mRNA were found. MTTP expression was higher in the HFSD group, and directly correlated with CM-TG and inversely correlated with CIF.ConclusionOur findings imply that gut TG content may constitute an important determinant of the secretion of TG-rich CMs, promoted by MTTP, with increased atherogenic potential.Fil: Olano, Carolina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; ArgentinaFil: Fariña, Gregorio. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Wiszniewski, Morena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Medel, Jimena. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiopatología Cardiovascular; ArgentinaFil: Morales, Celina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiopatología Cardiovascular; ArgentinaFil: Friedman, Silvia María. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Bioquímica General y Bucal; ArgentinaFil: Macri, Elisa Vanesa. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Bioquímica General y Bucal; ArgentinaFil: Barchuk, Magalí. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Berg, Gabriela Alicia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Schreier, Laura Ester. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; ArgentinaFil: Zago, Valeria. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaKorean Society of Lipid and Atherosclerosis2025-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/272439Olano, Carolina; Fariña, Gregorio; Wiszniewski, Morena; Medel, Jimena; Morales, Celina; et al.; Chylomicron Characteristics Are Associated With Microsomal Triglyceride Transfer Protein in an Animal Model of Diet-Induced Dysbiosis; Korean Society of Lipid and Atherosclerosis; Journal of Lipid and Atherosclerosis; 14; 1; 1-2025; 1-142287-28922288-2561CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://e-jla.org/DOIx.php?id=10.12997/jla.2025.14.1.106info:eu-repo/semantics/altIdentifier/doi/10.12997/jla.2025.14.1.106info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:06:12Zoai:ri.conicet.gov.ar:11336/272439instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:06:12.898CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Chylomicron Characteristics Are Associated With Microsomal Triglyceride Transfer Protein in an Animal Model of Diet-Induced Dysbiosis
title Chylomicron Characteristics Are Associated With Microsomal Triglyceride Transfer Protein in an Animal Model of Diet-Induced Dysbiosis
spellingShingle Chylomicron Characteristics Are Associated With Microsomal Triglyceride Transfer Protein in an Animal Model of Diet-Induced Dysbiosis
Olano, Carolina
Chylomicrons
Dysbiosis
Triglycerides
Atherogenic risk
title_short Chylomicron Characteristics Are Associated With Microsomal Triglyceride Transfer Protein in an Animal Model of Diet-Induced Dysbiosis
title_full Chylomicron Characteristics Are Associated With Microsomal Triglyceride Transfer Protein in an Animal Model of Diet-Induced Dysbiosis
title_fullStr Chylomicron Characteristics Are Associated With Microsomal Triglyceride Transfer Protein in an Animal Model of Diet-Induced Dysbiosis
title_full_unstemmed Chylomicron Characteristics Are Associated With Microsomal Triglyceride Transfer Protein in an Animal Model of Diet-Induced Dysbiosis
title_sort Chylomicron Characteristics Are Associated With Microsomal Triglyceride Transfer Protein in an Animal Model of Diet-Induced Dysbiosis
dc.creator.none.fl_str_mv Olano, Carolina
Fariña, Gregorio
Wiszniewski, Morena
Medel, Jimena
Morales, Celina
Friedman, Silvia María
Macri, Elisa Vanesa
Barchuk, Magalí
Berg, Gabriela Alicia
Schreier, Laura Ester
Zago, Valeria
author Olano, Carolina
author_facet Olano, Carolina
Fariña, Gregorio
Wiszniewski, Morena
Medel, Jimena
Morales, Celina
Friedman, Silvia María
Macri, Elisa Vanesa
Barchuk, Magalí
Berg, Gabriela Alicia
Schreier, Laura Ester
Zago, Valeria
author_role author
author2 Fariña, Gregorio
Wiszniewski, Morena
Medel, Jimena
Morales, Celina
Friedman, Silvia María
Macri, Elisa Vanesa
Barchuk, Magalí
Berg, Gabriela Alicia
Schreier, Laura Ester
Zago, Valeria
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Chylomicrons
Dysbiosis
Triglycerides
Atherogenic risk
topic Chylomicrons
Dysbiosis
Triglycerides
Atherogenic risk
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Objective: Intestinal remnant chylomicrons (CMs) are involved in cardiovascular residual risk and the atherogenic process. Microsomal triglyceride transfer protein (MTTP) catalyzes the assembly of lipids to apolipoprotein B48, generating CMs. Dysbiosis could alter this behavior. This study investigated the chemical composition of CMs and their associations with intestinal MTTP and gut fat depots in a diet-induced dysbiosis animal model.MethodsMale Wistar rats were fed either a standard diet (control, n=10) or a high-fat high-sucrose diet (HFSD, n=10) for 14 weeks. Measurements included serum glucose, lipid-lipoprotein profile, free fatty acids (FFAs), lipopolysaccharide (LPS) and the Firmicutes/Bacteroidetes (F/B) ratio in stool samples, via real-time quantitative polymerase chain reaction. Lipid content in isolated CMs (ultracentrifugation d <0.95 g/mL) was assessed, and MTTP, cell intestinal fat content (CIF), histology, apoB mRNA and tight junction (TJ) proteins were analyzed, in intestinal tissue.ResultsCompared to control, HFSD rats showed higher levels of LPS, triglycerides (TGs), non-high-density lipoprotein cholesterol (HDL-C) levels, TG/HDL-C ratio, FFAs, and the F/B ratio. HFSD CMs showed increased TG and phospholipids. TJ proteins levels were lower in the HFSD group, while histological scores showed no differences. CIF was increased in the HFSD group. No significant differences in apoB mRNA were found. MTTP expression was higher in the HFSD group, and directly correlated with CM-TG and inversely correlated with CIF.ConclusionOur findings imply that gut TG content may constitute an important determinant of the secretion of TG-rich CMs, promoted by MTTP, with increased atherogenic potential.
Fil: Olano, Carolina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
Fil: Fariña, Gregorio. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Wiszniewski, Morena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Medel, Jimena. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiopatología Cardiovascular; Argentina
Fil: Morales, Celina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiopatología Cardiovascular; Argentina
Fil: Friedman, Silvia María. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Bioquímica General y Bucal; Argentina
Fil: Macri, Elisa Vanesa. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Bioquímica General y Bucal; Argentina
Fil: Barchuk, Magalí. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Berg, Gabriela Alicia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Schreier, Laura Ester. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
Fil: Zago, Valeria. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Objective: Intestinal remnant chylomicrons (CMs) are involved in cardiovascular residual risk and the atherogenic process. Microsomal triglyceride transfer protein (MTTP) catalyzes the assembly of lipids to apolipoprotein B48, generating CMs. Dysbiosis could alter this behavior. This study investigated the chemical composition of CMs and their associations with intestinal MTTP and gut fat depots in a diet-induced dysbiosis animal model.MethodsMale Wistar rats were fed either a standard diet (control, n=10) or a high-fat high-sucrose diet (HFSD, n=10) for 14 weeks. Measurements included serum glucose, lipid-lipoprotein profile, free fatty acids (FFAs), lipopolysaccharide (LPS) and the Firmicutes/Bacteroidetes (F/B) ratio in stool samples, via real-time quantitative polymerase chain reaction. Lipid content in isolated CMs (ultracentrifugation d <0.95 g/mL) was assessed, and MTTP, cell intestinal fat content (CIF), histology, apoB mRNA and tight junction (TJ) proteins were analyzed, in intestinal tissue.ResultsCompared to control, HFSD rats showed higher levels of LPS, triglycerides (TGs), non-high-density lipoprotein cholesterol (HDL-C) levels, TG/HDL-C ratio, FFAs, and the F/B ratio. HFSD CMs showed increased TG and phospholipids. TJ proteins levels were lower in the HFSD group, while histological scores showed no differences. CIF was increased in the HFSD group. No significant differences in apoB mRNA were found. MTTP expression was higher in the HFSD group, and directly correlated with CM-TG and inversely correlated with CIF.ConclusionOur findings imply that gut TG content may constitute an important determinant of the secretion of TG-rich CMs, promoted by MTTP, with increased atherogenic potential.
publishDate 2025
dc.date.none.fl_str_mv 2025-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/272439
Olano, Carolina; Fariña, Gregorio; Wiszniewski, Morena; Medel, Jimena; Morales, Celina; et al.; Chylomicron Characteristics Are Associated With Microsomal Triglyceride Transfer Protein in an Animal Model of Diet-Induced Dysbiosis; Korean Society of Lipid and Atherosclerosis; Journal of Lipid and Atherosclerosis; 14; 1; 1-2025; 1-14
2287-2892
2288-2561
CONICET Digital
CONICET
url http://hdl.handle.net/11336/272439
identifier_str_mv Olano, Carolina; Fariña, Gregorio; Wiszniewski, Morena; Medel, Jimena; Morales, Celina; et al.; Chylomicron Characteristics Are Associated With Microsomal Triglyceride Transfer Protein in an Animal Model of Diet-Induced Dysbiosis; Korean Society of Lipid and Atherosclerosis; Journal of Lipid and Atherosclerosis; 14; 1; 1-2025; 1-14
2287-2892
2288-2561
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://e-jla.org/DOIx.php?id=10.12997/jla.2025.14.1.106
info:eu-repo/semantics/altIdentifier/doi/10.12997/jla.2025.14.1.106
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
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dc.publisher.none.fl_str_mv Korean Society of Lipid and Atherosclerosis
publisher.none.fl_str_mv Korean Society of Lipid and Atherosclerosis
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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