Skeletal muscle triglycerides, diacylglycerols, and ceramides in insulin resistance: Another paradox in endurance-trained athletes?
- Autores
- Amati, Francesca; Dubé, John J.; Alvarez Carnero, Elvis; Edreira, Martin Miguel; Chomentowski, Peter; Coen, Paul M.; Switzer, Galen E.; Bickel, Perry E.; Stefanovic Racic, Maja; Toledo, Frederico G. S.; Goodpaster, Bret H.
- Año de publicación
- 2011
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- OBJECTIVE - Chronic exercise and obesity both increase intramyocellular triglycerides (IMTGs) despite having opposing effects on insulin sensitivity. We hypothesized that chronically exercisetrained muscle would be characterized by lower skeletal muscle diacylglycerols (DAGs) and ceramides despite higher IMTGs and would account for its higher insulin sensitivity. We also hypothesized that the expression of key skeletal muscle proteins involved in lipid droplet hydrolysis, DAG formation, and fatty-acid partitioning and oxidation would be associated with the lipotoxic phenotype. RESEARCH DESIGN AND METHODS - A total of 14 normalweight, endurance-trained athletes (NWA group) and 7 normalweight sedentary (NWS group) and 21 obese sedentary (OBS group) volunteers were studied. Insulin sensitivity was assessed by glucose clamps. IMTGs, DAGs, ceramides, and protein expression were measured in muscle biopsies. RESULTS - DAG content in the NWA group was approximately twofold higher than in the OBS group and ~50% higher than in the NWS group, corresponding to higher insulin sensitivity. While certain DAG moieties clearly were associated with better insulin sensitivity, other species were not. Ceramide content was higher in insulin-resistant obese muscle. The expression of OXPAT/ perilipin-5, adipose triglyceride lipase, and stearoyl-CoA desaturase protein was higher in the NWA group, corresponding to a higher mitochondrial content, proportion of type 1 myocytes, IMTGs, DAGs, and insulin sensitivity. CONCLUSIONS - Total myocellular DAGs were markedly higher in highly trained athletes, corresponding with higher insulin sensitivity, and suggest a more complex role for DAGs in insulin action. Our data also provide additional evidence in humans linking ceramides to insulin resistance. Finally, this study provides novel evidence supporting a role for specific skeletal muscle proteins involved in intramyocellular lipids, mitochondrial oxidative capacity, and insulin resistance.
Fil: Amati, Francesca. University of Pittsburgh; Estados Unidos. Universite de Lausanne; Suiza
Fil: Dubé, John J.. University of Pittsburgh; Estados Unidos
Fil: Alvarez Carnero, Elvis. University of Pittsburgh; Estados Unidos
Fil: Edreira, Martin Miguel. University of Pittsburgh; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Chomentowski, Peter. University of Pittsburgh; Estados Unidos
Fil: Coen, Paul M.. University of Pittsburgh; Estados Unidos
Fil: Switzer, Galen E.. University of Pittsburgh; Estados Unidos. Center for Health Equity Research and Promotion; Estados Unidos
Fil: Bickel, Perry E.. University of Texas Health Science Center at Houston; Estados Unidos
Fil: Stefanovic Racic, Maja. University of Pittsburgh; Estados Unidos
Fil: Toledo, Frederico G. S.. University of Pittsburgh; Estados Unidos
Fil: Goodpaster, Bret H.. University of Pittsburgh; Estados Unidos - Materia
-
Triglycerides
Diacylglycerols
Ceramides
Insulin Resistance - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/79425
Ver los metadatos del registro completo
id |
CONICETDig_087665c94a15bcb8610c75ff8c4eb45b |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/79425 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Skeletal muscle triglycerides, diacylglycerols, and ceramides in insulin resistance: Another paradox in endurance-trained athletes?Amati, FrancescaDubé, John J.Alvarez Carnero, ElvisEdreira, Martin MiguelChomentowski, PeterCoen, Paul M.Switzer, Galen E.Bickel, Perry E.Stefanovic Racic, MajaToledo, Frederico G. S.Goodpaster, Bret H.TriglyceridesDiacylglycerolsCeramidesInsulin Resistancehttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3OBJECTIVE - Chronic exercise and obesity both increase intramyocellular triglycerides (IMTGs) despite having opposing effects on insulin sensitivity. We hypothesized that chronically exercisetrained muscle would be characterized by lower skeletal muscle diacylglycerols (DAGs) and ceramides despite higher IMTGs and would account for its higher insulin sensitivity. We also hypothesized that the expression of key skeletal muscle proteins involved in lipid droplet hydrolysis, DAG formation, and fatty-acid partitioning and oxidation would be associated with the lipotoxic phenotype. RESEARCH DESIGN AND METHODS - A total of 14 normalweight, endurance-trained athletes (NWA group) and 7 normalweight sedentary (NWS group) and 21 obese sedentary (OBS group) volunteers were studied. Insulin sensitivity was assessed by glucose clamps. IMTGs, DAGs, ceramides, and protein expression were measured in muscle biopsies. RESULTS - DAG content in the NWA group was approximately twofold higher than in the OBS group and ~50% higher than in the NWS group, corresponding to higher insulin sensitivity. While certain DAG moieties clearly were associated with better insulin sensitivity, other species were not. Ceramide content was higher in insulin-resistant obese muscle. The expression of OXPAT/ perilipin-5, adipose triglyceride lipase, and stearoyl-CoA desaturase protein was higher in the NWA group, corresponding to a higher mitochondrial content, proportion of type 1 myocytes, IMTGs, DAGs, and insulin sensitivity. CONCLUSIONS - Total myocellular DAGs were markedly higher in highly trained athletes, corresponding with higher insulin sensitivity, and suggest a more complex role for DAGs in insulin action. Our data also provide additional evidence in humans linking ceramides to insulin resistance. Finally, this study provides novel evidence supporting a role for specific skeletal muscle proteins involved in intramyocellular lipids, mitochondrial oxidative capacity, and insulin resistance.Fil: Amati, Francesca. University of Pittsburgh; Estados Unidos. Universite de Lausanne; SuizaFil: Dubé, John J.. University of Pittsburgh; Estados UnidosFil: Alvarez Carnero, Elvis. University of Pittsburgh; Estados UnidosFil: Edreira, Martin Miguel. University of Pittsburgh; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Chomentowski, Peter. University of Pittsburgh; Estados UnidosFil: Coen, Paul M.. University of Pittsburgh; Estados UnidosFil: Switzer, Galen E.. University of Pittsburgh; Estados Unidos. Center for Health Equity Research and Promotion; Estados UnidosFil: Bickel, Perry E.. University of Texas Health Science Center at Houston; Estados UnidosFil: Stefanovic Racic, Maja. University of Pittsburgh; Estados UnidosFil: Toledo, Frederico G. S.. University of Pittsburgh; Estados UnidosFil: Goodpaster, Bret H.. University of Pittsburgh; Estados UnidosAmerican Diabetes Association2011-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/79425Amati, Francesca; Dubé, John J.; Alvarez Carnero, Elvis; Edreira, Martin Miguel; Chomentowski, Peter; et al.; Skeletal muscle triglycerides, diacylglycerols, and ceramides in insulin resistance: Another paradox in endurance-trained athletes?; American Diabetes Association; Diabetes; 60; 10; 10-2011; 2588-25970012-1797CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21873552/info:eu-repo/semantics/altIdentifier/doi/10.2337/db10-1221info:eu-repo/semantics/altIdentifier/url/https://diabetes.diabetesjournals.org/content/60/10/2588info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:40:43Zoai:ri.conicet.gov.ar:11336/79425instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:40:43.445CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Skeletal muscle triglycerides, diacylglycerols, and ceramides in insulin resistance: Another paradox in endurance-trained athletes? |
title |
Skeletal muscle triglycerides, diacylglycerols, and ceramides in insulin resistance: Another paradox in endurance-trained athletes? |
spellingShingle |
Skeletal muscle triglycerides, diacylglycerols, and ceramides in insulin resistance: Another paradox in endurance-trained athletes? Amati, Francesca Triglycerides Diacylglycerols Ceramides Insulin Resistance |
title_short |
Skeletal muscle triglycerides, diacylglycerols, and ceramides in insulin resistance: Another paradox in endurance-trained athletes? |
title_full |
Skeletal muscle triglycerides, diacylglycerols, and ceramides in insulin resistance: Another paradox in endurance-trained athletes? |
title_fullStr |
Skeletal muscle triglycerides, diacylglycerols, and ceramides in insulin resistance: Another paradox in endurance-trained athletes? |
title_full_unstemmed |
Skeletal muscle triglycerides, diacylglycerols, and ceramides in insulin resistance: Another paradox in endurance-trained athletes? |
title_sort |
Skeletal muscle triglycerides, diacylglycerols, and ceramides in insulin resistance: Another paradox in endurance-trained athletes? |
dc.creator.none.fl_str_mv |
Amati, Francesca Dubé, John J. Alvarez Carnero, Elvis Edreira, Martin Miguel Chomentowski, Peter Coen, Paul M. Switzer, Galen E. Bickel, Perry E. Stefanovic Racic, Maja Toledo, Frederico G. S. Goodpaster, Bret H. |
author |
Amati, Francesca |
author_facet |
Amati, Francesca Dubé, John J. Alvarez Carnero, Elvis Edreira, Martin Miguel Chomentowski, Peter Coen, Paul M. Switzer, Galen E. Bickel, Perry E. Stefanovic Racic, Maja Toledo, Frederico G. S. Goodpaster, Bret H. |
author_role |
author |
author2 |
Dubé, John J. Alvarez Carnero, Elvis Edreira, Martin Miguel Chomentowski, Peter Coen, Paul M. Switzer, Galen E. Bickel, Perry E. Stefanovic Racic, Maja Toledo, Frederico G. S. Goodpaster, Bret H. |
author2_role |
author author author author author author author author author author |
dc.subject.none.fl_str_mv |
Triglycerides Diacylglycerols Ceramides Insulin Resistance |
topic |
Triglycerides Diacylglycerols Ceramides Insulin Resistance |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.2 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
OBJECTIVE - Chronic exercise and obesity both increase intramyocellular triglycerides (IMTGs) despite having opposing effects on insulin sensitivity. We hypothesized that chronically exercisetrained muscle would be characterized by lower skeletal muscle diacylglycerols (DAGs) and ceramides despite higher IMTGs and would account for its higher insulin sensitivity. We also hypothesized that the expression of key skeletal muscle proteins involved in lipid droplet hydrolysis, DAG formation, and fatty-acid partitioning and oxidation would be associated with the lipotoxic phenotype. RESEARCH DESIGN AND METHODS - A total of 14 normalweight, endurance-trained athletes (NWA group) and 7 normalweight sedentary (NWS group) and 21 obese sedentary (OBS group) volunteers were studied. Insulin sensitivity was assessed by glucose clamps. IMTGs, DAGs, ceramides, and protein expression were measured in muscle biopsies. RESULTS - DAG content in the NWA group was approximately twofold higher than in the OBS group and ~50% higher than in the NWS group, corresponding to higher insulin sensitivity. While certain DAG moieties clearly were associated with better insulin sensitivity, other species were not. Ceramide content was higher in insulin-resistant obese muscle. The expression of OXPAT/ perilipin-5, adipose triglyceride lipase, and stearoyl-CoA desaturase protein was higher in the NWA group, corresponding to a higher mitochondrial content, proportion of type 1 myocytes, IMTGs, DAGs, and insulin sensitivity. CONCLUSIONS - Total myocellular DAGs were markedly higher in highly trained athletes, corresponding with higher insulin sensitivity, and suggest a more complex role for DAGs in insulin action. Our data also provide additional evidence in humans linking ceramides to insulin resistance. Finally, this study provides novel evidence supporting a role for specific skeletal muscle proteins involved in intramyocellular lipids, mitochondrial oxidative capacity, and insulin resistance. Fil: Amati, Francesca. University of Pittsburgh; Estados Unidos. Universite de Lausanne; Suiza Fil: Dubé, John J.. University of Pittsburgh; Estados Unidos Fil: Alvarez Carnero, Elvis. University of Pittsburgh; Estados Unidos Fil: Edreira, Martin Miguel. University of Pittsburgh; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina Fil: Chomentowski, Peter. University of Pittsburgh; Estados Unidos Fil: Coen, Paul M.. University of Pittsburgh; Estados Unidos Fil: Switzer, Galen E.. University of Pittsburgh; Estados Unidos. Center for Health Equity Research and Promotion; Estados Unidos Fil: Bickel, Perry E.. University of Texas Health Science Center at Houston; Estados Unidos Fil: Stefanovic Racic, Maja. University of Pittsburgh; Estados Unidos Fil: Toledo, Frederico G. S.. University of Pittsburgh; Estados Unidos Fil: Goodpaster, Bret H.. University of Pittsburgh; Estados Unidos |
description |
OBJECTIVE - Chronic exercise and obesity both increase intramyocellular triglycerides (IMTGs) despite having opposing effects on insulin sensitivity. We hypothesized that chronically exercisetrained muscle would be characterized by lower skeletal muscle diacylglycerols (DAGs) and ceramides despite higher IMTGs and would account for its higher insulin sensitivity. We also hypothesized that the expression of key skeletal muscle proteins involved in lipid droplet hydrolysis, DAG formation, and fatty-acid partitioning and oxidation would be associated with the lipotoxic phenotype. RESEARCH DESIGN AND METHODS - A total of 14 normalweight, endurance-trained athletes (NWA group) and 7 normalweight sedentary (NWS group) and 21 obese sedentary (OBS group) volunteers were studied. Insulin sensitivity was assessed by glucose clamps. IMTGs, DAGs, ceramides, and protein expression were measured in muscle biopsies. RESULTS - DAG content in the NWA group was approximately twofold higher than in the OBS group and ~50% higher than in the NWS group, corresponding to higher insulin sensitivity. While certain DAG moieties clearly were associated with better insulin sensitivity, other species were not. Ceramide content was higher in insulin-resistant obese muscle. The expression of OXPAT/ perilipin-5, adipose triglyceride lipase, and stearoyl-CoA desaturase protein was higher in the NWA group, corresponding to a higher mitochondrial content, proportion of type 1 myocytes, IMTGs, DAGs, and insulin sensitivity. CONCLUSIONS - Total myocellular DAGs were markedly higher in highly trained athletes, corresponding with higher insulin sensitivity, and suggest a more complex role for DAGs in insulin action. Our data also provide additional evidence in humans linking ceramides to insulin resistance. Finally, this study provides novel evidence supporting a role for specific skeletal muscle proteins involved in intramyocellular lipids, mitochondrial oxidative capacity, and insulin resistance. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-10 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/79425 Amati, Francesca; Dubé, John J.; Alvarez Carnero, Elvis; Edreira, Martin Miguel; Chomentowski, Peter; et al.; Skeletal muscle triglycerides, diacylglycerols, and ceramides in insulin resistance: Another paradox in endurance-trained athletes?; American Diabetes Association; Diabetes; 60; 10; 10-2011; 2588-2597 0012-1797 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/79425 |
identifier_str_mv |
Amati, Francesca; Dubé, John J.; Alvarez Carnero, Elvis; Edreira, Martin Miguel; Chomentowski, Peter; et al.; Skeletal muscle triglycerides, diacylglycerols, and ceramides in insulin resistance: Another paradox in endurance-trained athletes?; American Diabetes Association; Diabetes; 60; 10; 10-2011; 2588-2597 0012-1797 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21873552/ info:eu-repo/semantics/altIdentifier/doi/10.2337/db10-1221 info:eu-repo/semantics/altIdentifier/url/https://diabetes.diabetesjournals.org/content/60/10/2588 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
American Diabetes Association |
publisher.none.fl_str_mv |
American Diabetes Association |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844614435942957056 |
score |
13.070432 |