Preliminary studies on the biobleaching of loblolly pine Kraft pulp with Trametes trogii crude extracts

Autores
Da Re, Cristina Verónica; Papinutti, Víctor Leandro; Villalba, Laura; Forchiassin, Flavia; Levin, Laura Noemí
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Trametes trogii (BAFC 463), Coriolus versicolor var. antarcticus (BAFC 266) and Trametes villosa (BAFC 2755) are Argentinean white-rot fungi that proved to be efficient ligninolytic enzyme producers in previous studies. The aim of the present work was investigating the ability of their crude extracellular extracts for the biobleaching of loblolly pine Kraft pulp. The production of laccase, manganese peroxidase, endoxylanase and endoglucanase was also evaluated. Out of the three fungi studied, the best biobleaching results were obtained with culture supernatants from T. trogii grown for 21 days in a synthetic medium with glucose and asparagine as carbon and nitrogen sources, respectively, with the addition of 1 mM copper sulfate. Such medium rendered high level of ligninolytic enzymes: 0.25 U ml-1 of manganese peroxidase and 44 U ml-1 of laccase. A strong brightness increase was attained after the enzymatic treatment followed by H2O2. Bleaching up to 82% ISO brightness (compared with 37% in the peroxide-bleached control) was obtained. Moreover, after the enzymatic treatment, hydrogen peroxide requirements decreased (as reflected by the higher residual peroxide), and the pulp luminance reached a value of 92.5. Addition of known laccase mediators such as HBT, ABTS, and HBA did not improve the process. The supernatant did not significantly lose its bleaching capacity even after successive recovery and reapplication to unbleached pulp. Correspondingly, laccase and manganese peroxidase activities in the recovered supernatant, exhibited near 100% of their initial values.
Fil: Da Re, Cristina Verónica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Micología Experimental; Argentina
Fil: Papinutti, Víctor Leandro. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Micología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Villalba, Laura. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina
Fil: Forchiassin, Flavia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Micología Experimental; Argentina
Fil: Levin, Laura Noemí. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Micología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Biobleaching
Enzyme Stability
Kraft Pulp
Laccase
Mediator
Xylanase
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/37640
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/37640
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Preliminary studies on the biobleaching of loblolly pine Kraft pulp with Trametes trogii crude extractsDa Re, Cristina VerónicaPapinutti, Víctor LeandroVillalba, LauraForchiassin, FlaviaLevin, Laura NoemíBiobleachingEnzyme StabilityKraft PulpLaccaseMediatorXylanasehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Trametes trogii (BAFC 463), Coriolus versicolor var. antarcticus (BAFC 266) and Trametes villosa (BAFC 2755) are Argentinean white-rot fungi that proved to be efficient ligninolytic enzyme producers in previous studies. The aim of the present work was investigating the ability of their crude extracellular extracts for the biobleaching of loblolly pine Kraft pulp. The production of laccase, manganese peroxidase, endoxylanase and endoglucanase was also evaluated. Out of the three fungi studied, the best biobleaching results were obtained with culture supernatants from T. trogii grown for 21 days in a synthetic medium with glucose and asparagine as carbon and nitrogen sources, respectively, with the addition of 1 mM copper sulfate. Such medium rendered high level of ligninolytic enzymes: 0.25 U ml-1 of manganese peroxidase and 44 U ml-1 of laccase. A strong brightness increase was attained after the enzymatic treatment followed by H2O2. Bleaching up to 82% ISO brightness (compared with 37% in the peroxide-bleached control) was obtained. Moreover, after the enzymatic treatment, hydrogen peroxide requirements decreased (as reflected by the higher residual peroxide), and the pulp luminance reached a value of 92.5. Addition of known laccase mediators such as HBT, ABTS, and HBA did not improve the process. The supernatant did not significantly lose its bleaching capacity even after successive recovery and reapplication to unbleached pulp. Correspondingly, laccase and manganese peroxidase activities in the recovered supernatant, exhibited near 100% of their initial values.Fil: Da Re, Cristina Verónica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Micología Experimental; ArgentinaFil: Papinutti, Víctor Leandro. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Micología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Villalba, Laura. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; ArgentinaFil: Forchiassin, Flavia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Micología Experimental; ArgentinaFil: Levin, Laura Noemí. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Micología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Science Inc2008-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/37640Da Re, Cristina Verónica; Papinutti, Víctor Leandro; Villalba, Laura; Forchiassin, Flavia; Levin, Laura Noemí; Preliminary studies on the biobleaching of loblolly pine Kraft pulp with Trametes trogii crude extracts; Elsevier Science Inc; Enzyme And Microbial Technology; 43; 2; 8-2008; 164-1680141-0229CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.enzmictec.2007.11.004info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0141022907003493info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:28:35Zoai:ri.conicet.gov.ar:11336/37640instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:28:35.413CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Preliminary studies on the biobleaching of loblolly pine Kraft pulp with Trametes trogii crude extracts
title Preliminary studies on the biobleaching of loblolly pine Kraft pulp with Trametes trogii crude extracts
spellingShingle Preliminary studies on the biobleaching of loblolly pine Kraft pulp with Trametes trogii crude extracts
Da Re, Cristina Verónica
Biobleaching
Enzyme Stability
Kraft Pulp
Laccase
Mediator
Xylanase
title_short Preliminary studies on the biobleaching of loblolly pine Kraft pulp with Trametes trogii crude extracts
title_full Preliminary studies on the biobleaching of loblolly pine Kraft pulp with Trametes trogii crude extracts
title_fullStr Preliminary studies on the biobleaching of loblolly pine Kraft pulp with Trametes trogii crude extracts
title_full_unstemmed Preliminary studies on the biobleaching of loblolly pine Kraft pulp with Trametes trogii crude extracts
title_sort Preliminary studies on the biobleaching of loblolly pine Kraft pulp with Trametes trogii crude extracts
dc.creator.none.fl_str_mv Da Re, Cristina Verónica
Papinutti, Víctor Leandro
Villalba, Laura
Forchiassin, Flavia
Levin, Laura Noemí
author Da Re, Cristina Verónica
author_facet Da Re, Cristina Verónica
Papinutti, Víctor Leandro
Villalba, Laura
Forchiassin, Flavia
Levin, Laura Noemí
author_role author
author2 Papinutti, Víctor Leandro
Villalba, Laura
Forchiassin, Flavia
Levin, Laura Noemí
author2_role author
author
author
author
dc.subject.none.fl_str_mv Biobleaching
Enzyme Stability
Kraft Pulp
Laccase
Mediator
Xylanase
topic Biobleaching
Enzyme Stability
Kraft Pulp
Laccase
Mediator
Xylanase
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Trametes trogii (BAFC 463), Coriolus versicolor var. antarcticus (BAFC 266) and Trametes villosa (BAFC 2755) are Argentinean white-rot fungi that proved to be efficient ligninolytic enzyme producers in previous studies. The aim of the present work was investigating the ability of their crude extracellular extracts for the biobleaching of loblolly pine Kraft pulp. The production of laccase, manganese peroxidase, endoxylanase and endoglucanase was also evaluated. Out of the three fungi studied, the best biobleaching results were obtained with culture supernatants from T. trogii grown for 21 days in a synthetic medium with glucose and asparagine as carbon and nitrogen sources, respectively, with the addition of 1 mM copper sulfate. Such medium rendered high level of ligninolytic enzymes: 0.25 U ml-1 of manganese peroxidase and 44 U ml-1 of laccase. A strong brightness increase was attained after the enzymatic treatment followed by H2O2. Bleaching up to 82% ISO brightness (compared with 37% in the peroxide-bleached control) was obtained. Moreover, after the enzymatic treatment, hydrogen peroxide requirements decreased (as reflected by the higher residual peroxide), and the pulp luminance reached a value of 92.5. Addition of known laccase mediators such as HBT, ABTS, and HBA did not improve the process. The supernatant did not significantly lose its bleaching capacity even after successive recovery and reapplication to unbleached pulp. Correspondingly, laccase and manganese peroxidase activities in the recovered supernatant, exhibited near 100% of their initial values.
Fil: Da Re, Cristina Verónica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Micología Experimental; Argentina
Fil: Papinutti, Víctor Leandro. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Micología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Villalba, Laura. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina
Fil: Forchiassin, Flavia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Micología Experimental; Argentina
Fil: Levin, Laura Noemí. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Micología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Trametes trogii (BAFC 463), Coriolus versicolor var. antarcticus (BAFC 266) and Trametes villosa (BAFC 2755) are Argentinean white-rot fungi that proved to be efficient ligninolytic enzyme producers in previous studies. The aim of the present work was investigating the ability of their crude extracellular extracts for the biobleaching of loblolly pine Kraft pulp. The production of laccase, manganese peroxidase, endoxylanase and endoglucanase was also evaluated. Out of the three fungi studied, the best biobleaching results were obtained with culture supernatants from T. trogii grown for 21 days in a synthetic medium with glucose and asparagine as carbon and nitrogen sources, respectively, with the addition of 1 mM copper sulfate. Such medium rendered high level of ligninolytic enzymes: 0.25 U ml-1 of manganese peroxidase and 44 U ml-1 of laccase. A strong brightness increase was attained after the enzymatic treatment followed by H2O2. Bleaching up to 82% ISO brightness (compared with 37% in the peroxide-bleached control) was obtained. Moreover, after the enzymatic treatment, hydrogen peroxide requirements decreased (as reflected by the higher residual peroxide), and the pulp luminance reached a value of 92.5. Addition of known laccase mediators such as HBT, ABTS, and HBA did not improve the process. The supernatant did not significantly lose its bleaching capacity even after successive recovery and reapplication to unbleached pulp. Correspondingly, laccase and manganese peroxidase activities in the recovered supernatant, exhibited near 100% of their initial values.
publishDate 2008
dc.date.none.fl_str_mv 2008-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/37640
Da Re, Cristina Verónica; Papinutti, Víctor Leandro; Villalba, Laura; Forchiassin, Flavia; Levin, Laura Noemí; Preliminary studies on the biobleaching of loblolly pine Kraft pulp with Trametes trogii crude extracts; Elsevier Science Inc; Enzyme And Microbial Technology; 43; 2; 8-2008; 164-168
0141-0229
CONICET Digital
CONICET
url http://hdl.handle.net/11336/37640
identifier_str_mv Da Re, Cristina Verónica; Papinutti, Víctor Leandro; Villalba, Laura; Forchiassin, Flavia; Levin, Laura Noemí; Preliminary studies on the biobleaching of loblolly pine Kraft pulp with Trametes trogii crude extracts; Elsevier Science Inc; Enzyme And Microbial Technology; 43; 2; 8-2008; 164-168
0141-0229
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.enzmictec.2007.11.004
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0141022907003493
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Inc
publisher.none.fl_str_mv Elsevier Science Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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