Biobleaching of loblolly pine kraft pulp with Trametes trogii culture fluids followed by a peroxide stage: application of Doehlert experimental design to evaluate process parameter...
- Autores
- Da Re, Cristina Verónica; Papinutti, Víctor Leandro; Forchiassin, Flavia; Levin, Laura Noemí
- Año de publicación
- 2009
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Loblolly pine kraft pulp was bleached in a totally chlorine-free sequence that involved treatment with culture supernatants from the white-rot fungus Trametes trogii followed by a peroxide stage. The whole process was performed at 28 °C, and did not require mediator addition in the enzymatic stage. Different operating conditions in the peroxide stage (pH, peroxide concentration and treatment time), were tested by using response surface methodology based on a Doehlert experimental design, in order to describe their effects and normalize a biobleaching protocol. The results showed that all three independent variables had significant effect on the luminance (L*) and Chroma (C*) of the enzyme-treated pulp. Best results were obtained after 1 h of enzyme incubation (352 U laccase, 2 U Mn-peroxidase per g of oven-dry pulp), followed by 96 h treatment with 2.5% hydrogen peroxide in sodium succinate buffer pH 6 (5% consistency). We obtained a noteworthy increase in L* = 94.45 (compared with 94.5 of the white reference standard (titanium oxide), 69.94 of the initial pulp, and 83.11 of the peroxide-bleached control), a decrease in C* (9.85), with minor pulp yield loss (less than 5%), under essentially mild conditions, using a low-cost source of enzyme.
Fil: Da Re, Cristina Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina
Fil: Papinutti, Víctor Leandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina
Fil: Forchiassin, Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina
Fil: Levin, Laura Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina - Materia
-
Softwood Kraft Pulp
Trames Trogii
Laccase
Mn Peroxidase - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/26928
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oai:ri.conicet.gov.ar:11336/26928 |
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CONICET Digital (CONICET) |
spelling |
Biobleaching of loblolly pine kraft pulp with Trametes trogii culture fluids followed by a peroxide stage: application of Doehlert experimental design to evaluate process parametersDa Re, Cristina VerónicaPapinutti, Víctor LeandroForchiassin, FlaviaLevin, Laura NoemíSoftwood Kraft PulpTrames TrogiiLaccaseMn Peroxidasehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Loblolly pine kraft pulp was bleached in a totally chlorine-free sequence that involved treatment with culture supernatants from the white-rot fungus Trametes trogii followed by a peroxide stage. The whole process was performed at 28 °C, and did not require mediator addition in the enzymatic stage. Different operating conditions in the peroxide stage (pH, peroxide concentration and treatment time), were tested by using response surface methodology based on a Doehlert experimental design, in order to describe their effects and normalize a biobleaching protocol. The results showed that all three independent variables had significant effect on the luminance (L*) and Chroma (C*) of the enzyme-treated pulp. Best results were obtained after 1 h of enzyme incubation (352 U laccase, 2 U Mn-peroxidase per g of oven-dry pulp), followed by 96 h treatment with 2.5% hydrogen peroxide in sodium succinate buffer pH 6 (5% consistency). We obtained a noteworthy increase in L* = 94.45 (compared with 94.5 of the white reference standard (titanium oxide), 69.94 of the initial pulp, and 83.11 of the peroxide-bleached control), a decrease in C* (9.85), with minor pulp yield loss (less than 5%), under essentially mild conditions, using a low-cost source of enzyme.Fil: Da Re, Cristina Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; ArgentinaFil: Papinutti, Víctor Leandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; ArgentinaFil: Forchiassin, Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; ArgentinaFil: Levin, Laura Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; ArgentinaElsevier Science Inc2009-12-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/26928Da Re, Cristina Verónica; Papinutti, Víctor Leandro; Forchiassin, Flavia; Levin, Laura Noemí; Biobleaching of loblolly pine kraft pulp with Trametes trogii culture fluids followed by a peroxide stage: application of Doehlert experimental design to evaluate process parameters; Elsevier Science Inc; Enzyme And Microbial Technology; 46; 3-4; 28-12-2009; 281-2860141-02291879-0909CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0141022909002828info:eu-repo/semantics/altIdentifier/doi/10.1016/j.enzmictec.2009.12.006info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:44:09Zoai:ri.conicet.gov.ar:11336/26928instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:44:09.834CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Biobleaching of loblolly pine kraft pulp with Trametes trogii culture fluids followed by a peroxide stage: application of Doehlert experimental design to evaluate process parameters |
title |
Biobleaching of loblolly pine kraft pulp with Trametes trogii culture fluids followed by a peroxide stage: application of Doehlert experimental design to evaluate process parameters |
spellingShingle |
Biobleaching of loblolly pine kraft pulp with Trametes trogii culture fluids followed by a peroxide stage: application of Doehlert experimental design to evaluate process parameters Da Re, Cristina Verónica Softwood Kraft Pulp Trames Trogii Laccase Mn Peroxidase |
title_short |
Biobleaching of loblolly pine kraft pulp with Trametes trogii culture fluids followed by a peroxide stage: application of Doehlert experimental design to evaluate process parameters |
title_full |
Biobleaching of loblolly pine kraft pulp with Trametes trogii culture fluids followed by a peroxide stage: application of Doehlert experimental design to evaluate process parameters |
title_fullStr |
Biobleaching of loblolly pine kraft pulp with Trametes trogii culture fluids followed by a peroxide stage: application of Doehlert experimental design to evaluate process parameters |
title_full_unstemmed |
Biobleaching of loblolly pine kraft pulp with Trametes trogii culture fluids followed by a peroxide stage: application of Doehlert experimental design to evaluate process parameters |
title_sort |
Biobleaching of loblolly pine kraft pulp with Trametes trogii culture fluids followed by a peroxide stage: application of Doehlert experimental design to evaluate process parameters |
dc.creator.none.fl_str_mv |
Da Re, Cristina Verónica Papinutti, Víctor Leandro Forchiassin, Flavia Levin, Laura Noemí |
author |
Da Re, Cristina Verónica |
author_facet |
Da Re, Cristina Verónica Papinutti, Víctor Leandro Forchiassin, Flavia Levin, Laura Noemí |
author_role |
author |
author2 |
Papinutti, Víctor Leandro Forchiassin, Flavia Levin, Laura Noemí |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Softwood Kraft Pulp Trames Trogii Laccase Mn Peroxidase |
topic |
Softwood Kraft Pulp Trames Trogii Laccase Mn Peroxidase |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Loblolly pine kraft pulp was bleached in a totally chlorine-free sequence that involved treatment with culture supernatants from the white-rot fungus Trametes trogii followed by a peroxide stage. The whole process was performed at 28 °C, and did not require mediator addition in the enzymatic stage. Different operating conditions in the peroxide stage (pH, peroxide concentration and treatment time), were tested by using response surface methodology based on a Doehlert experimental design, in order to describe their effects and normalize a biobleaching protocol. The results showed that all three independent variables had significant effect on the luminance (L*) and Chroma (C*) of the enzyme-treated pulp. Best results were obtained after 1 h of enzyme incubation (352 U laccase, 2 U Mn-peroxidase per g of oven-dry pulp), followed by 96 h treatment with 2.5% hydrogen peroxide in sodium succinate buffer pH 6 (5% consistency). We obtained a noteworthy increase in L* = 94.45 (compared with 94.5 of the white reference standard (titanium oxide), 69.94 of the initial pulp, and 83.11 of the peroxide-bleached control), a decrease in C* (9.85), with minor pulp yield loss (less than 5%), under essentially mild conditions, using a low-cost source of enzyme. Fil: Da Re, Cristina Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina Fil: Papinutti, Víctor Leandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina Fil: Forchiassin, Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina Fil: Levin, Laura Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina |
description |
Loblolly pine kraft pulp was bleached in a totally chlorine-free sequence that involved treatment with culture supernatants from the white-rot fungus Trametes trogii followed by a peroxide stage. The whole process was performed at 28 °C, and did not require mediator addition in the enzymatic stage. Different operating conditions in the peroxide stage (pH, peroxide concentration and treatment time), were tested by using response surface methodology based on a Doehlert experimental design, in order to describe their effects and normalize a biobleaching protocol. The results showed that all three independent variables had significant effect on the luminance (L*) and Chroma (C*) of the enzyme-treated pulp. Best results were obtained after 1 h of enzyme incubation (352 U laccase, 2 U Mn-peroxidase per g of oven-dry pulp), followed by 96 h treatment with 2.5% hydrogen peroxide in sodium succinate buffer pH 6 (5% consistency). We obtained a noteworthy increase in L* = 94.45 (compared with 94.5 of the white reference standard (titanium oxide), 69.94 of the initial pulp, and 83.11 of the peroxide-bleached control), a decrease in C* (9.85), with minor pulp yield loss (less than 5%), under essentially mild conditions, using a low-cost source of enzyme. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-12-28 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/26928 Da Re, Cristina Verónica; Papinutti, Víctor Leandro; Forchiassin, Flavia; Levin, Laura Noemí; Biobleaching of loblolly pine kraft pulp with Trametes trogii culture fluids followed by a peroxide stage: application of Doehlert experimental design to evaluate process parameters; Elsevier Science Inc; Enzyme And Microbial Technology; 46; 3-4; 28-12-2009; 281-286 0141-0229 1879-0909 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/26928 |
identifier_str_mv |
Da Re, Cristina Verónica; Papinutti, Víctor Leandro; Forchiassin, Flavia; Levin, Laura Noemí; Biobleaching of loblolly pine kraft pulp with Trametes trogii culture fluids followed by a peroxide stage: application of Doehlert experimental design to evaluate process parameters; Elsevier Science Inc; Enzyme And Microbial Technology; 46; 3-4; 28-12-2009; 281-286 0141-0229 1879-0909 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0141022909002828 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.enzmictec.2009.12.006 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Science Inc |
publisher.none.fl_str_mv |
Elsevier Science Inc |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1846083544914329600 |
score |
13.22299 |