Laboratory Evaluations of Syzygium aromaticum (L.) Merr. et Perry Essential Oil Against Varroa destructor

Autores
Maggi, Matías Daniel; Ruffnengo, Sergio R.; Gende, Liesel Brenda; Sarlo, Edgardo G.; Eguaras, Martin Javier; Bailac, Pedro Nelson; Ponzi, Marta Isabel
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The oil obtained by hydrodistillation of the floral bottom of Syzygium aromaticum (L.) Merr. et Perry was analyzed by GC and GC/MS. Eugenol was the main constituent in the oil (86.7%). The biological activity of the oil applied to Varroa destructor and Apis mellifera was evaluated in two laboratory tests. Mite lethality was estimated using a complete exposure method test with the oil at different concentrations, and a systemic administration method of oil at different concentrations diluted in syrup was placed in feeders for bees. The LC50 for complete exposure method at 24 h was 0.59 mL/dish. The inferior and superior limits obtained were 0.47 x 10-6 mL/dish and 1.22 mL/dish, respectively. LC50 estimated at 48 h showed a slight decrease as compared to that recorded at 24 h. Ratio selection (LC50 of A. mellifera/ LC50 of V. destructor) for complete exposure method was 26.46 and 13.35 for 24 h and 48 h, respectively. Regarding the systemic administration method, mites LC50 at 24 h was 12,300 ppm. The inferior and superior limits calculated were 9,214 ppm and 15,178 ppm, respectively. LC50 estimated at 48 h showed a slight decrease as compared to that recorded at 24 h. Ratio selection for systemic administration method was 3.05 and 2.22 for 24 h and 48 h, respectively. Syzygium aromaticum oil was found to be an attractant for V. destructor at 4.8% (w/w) concentration. The results showed that oil toxicity against V. destructor differed depending upon its administration. Nevertheless, the ratio selection calculated by this oil is expected to enable its application under field conditions with a good safety margin. This oil could also be used in combination with other oils in integrated pest management strategies in bee colonies.
Fil: Maggi, Matías Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología. Laboratorio de Artrópodos; Argentina
Fil: Ruffnengo, Sergio R.. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología. Laboratorio de Artrópodos; Argentina
Fil: Gende, Liesel Brenda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología. Laboratorio de Artrópodos; Argentina
Fil: Sarlo, Edgardo G.. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología. Laboratorio de Artrópodos; Argentina
Fil: Eguaras, Martin Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología. Laboratorio de Artrópodos; Argentina
Fil: Bailac, Pedro Nelson. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina
Fil: Ponzi, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina
Materia
Syzygium aromaticum
Myrtaceae
Essential oil composition
Eugenol
Mite lethality lead
Apis Mellifera
Varroa destructor
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/242407
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/242407
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Laboratory Evaluations of Syzygium aromaticum (L.) Merr. et Perry Essential Oil Against Varroa destructorMaggi, Matías DanielRuffnengo, Sergio R.Gende, Liesel BrendaSarlo, Edgardo G.Eguaras, Martin JavierBailac, Pedro NelsonPonzi, Marta IsabelSyzygium aromaticumMyrtaceaeEssential oil compositionEugenolMite lethality leadApis MelliferaVarroa destructorhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The oil obtained by hydrodistillation of the floral bottom of Syzygium aromaticum (L.) Merr. et Perry was analyzed by GC and GC/MS. Eugenol was the main constituent in the oil (86.7%). The biological activity of the oil applied to Varroa destructor and Apis mellifera was evaluated in two laboratory tests. Mite lethality was estimated using a complete exposure method test with the oil at different concentrations, and a systemic administration method of oil at different concentrations diluted in syrup was placed in feeders for bees. The LC50 for complete exposure method at 24 h was 0.59 mL/dish. The inferior and superior limits obtained were 0.47 x 10-6 mL/dish and 1.22 mL/dish, respectively. LC50 estimated at 48 h showed a slight decrease as compared to that recorded at 24 h. Ratio selection (LC50 of A. mellifera/ LC50 of V. destructor) for complete exposure method was 26.46 and 13.35 for 24 h and 48 h, respectively. Regarding the systemic administration method, mites LC50 at 24 h was 12,300 ppm. The inferior and superior limits calculated were 9,214 ppm and 15,178 ppm, respectively. LC50 estimated at 48 h showed a slight decrease as compared to that recorded at 24 h. Ratio selection for systemic administration method was 3.05 and 2.22 for 24 h and 48 h, respectively. Syzygium aromaticum oil was found to be an attractant for V. destructor at 4.8% (w/w) concentration. The results showed that oil toxicity against V. destructor differed depending upon its administration. Nevertheless, the ratio selection calculated by this oil is expected to enable its application under field conditions with a good safety margin. This oil could also be used in combination with other oils in integrated pest management strategies in bee colonies.Fil: Maggi, Matías Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología. Laboratorio de Artrópodos; ArgentinaFil: Ruffnengo, Sergio R.. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología. Laboratorio de Artrópodos; ArgentinaFil: Gende, Liesel Brenda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología. Laboratorio de Artrópodos; ArgentinaFil: Sarlo, Edgardo G.. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología. Laboratorio de Artrópodos; ArgentinaFil: Eguaras, Martin Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología. Laboratorio de Artrópodos; ArgentinaFil: Bailac, Pedro Nelson. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; ArgentinaFil: Ponzi, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; ArgentinaAllured Publishing Corporation2010-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/242407Maggi, Matías Daniel; Ruffnengo, Sergio R.; Gende, Liesel Brenda; Sarlo, Edgardo G.; Eguaras, Martin Javier; et al.; Laboratory Evaluations of Syzygium aromaticum (L.) Merr. et Perry Essential Oil Against Varroa destructor; Allured Publishing Corporation; Journal of Essential Oil Research; 22; 2; 3-2010; 119-1221041-2905CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/abs/10.1080/10412905.2010.9700278info:eu-repo/semantics/altIdentifier/doi/10.1080/10412905.2010.9700278info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:35:17Zoai:ri.conicet.gov.ar:11336/242407instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:35:17.794CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Laboratory Evaluations of Syzygium aromaticum (L.) Merr. et Perry Essential Oil Against Varroa destructor
title Laboratory Evaluations of Syzygium aromaticum (L.) Merr. et Perry Essential Oil Against Varroa destructor
spellingShingle Laboratory Evaluations of Syzygium aromaticum (L.) Merr. et Perry Essential Oil Against Varroa destructor
Maggi, Matías Daniel
Syzygium aromaticum
Myrtaceae
Essential oil composition
Eugenol
Mite lethality lead
Apis Mellifera
Varroa destructor
title_short Laboratory Evaluations of Syzygium aromaticum (L.) Merr. et Perry Essential Oil Against Varroa destructor
title_full Laboratory Evaluations of Syzygium aromaticum (L.) Merr. et Perry Essential Oil Against Varroa destructor
title_fullStr Laboratory Evaluations of Syzygium aromaticum (L.) Merr. et Perry Essential Oil Against Varroa destructor
title_full_unstemmed Laboratory Evaluations of Syzygium aromaticum (L.) Merr. et Perry Essential Oil Against Varroa destructor
title_sort Laboratory Evaluations of Syzygium aromaticum (L.) Merr. et Perry Essential Oil Against Varroa destructor
dc.creator.none.fl_str_mv Maggi, Matías Daniel
Ruffnengo, Sergio R.
Gende, Liesel Brenda
Sarlo, Edgardo G.
Eguaras, Martin Javier
Bailac, Pedro Nelson
Ponzi, Marta Isabel
author Maggi, Matías Daniel
author_facet Maggi, Matías Daniel
Ruffnengo, Sergio R.
Gende, Liesel Brenda
Sarlo, Edgardo G.
Eguaras, Martin Javier
Bailac, Pedro Nelson
Ponzi, Marta Isabel
author_role author
author2 Ruffnengo, Sergio R.
Gende, Liesel Brenda
Sarlo, Edgardo G.
Eguaras, Martin Javier
Bailac, Pedro Nelson
Ponzi, Marta Isabel
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Syzygium aromaticum
Myrtaceae
Essential oil composition
Eugenol
Mite lethality lead
Apis Mellifera
Varroa destructor
topic Syzygium aromaticum
Myrtaceae
Essential oil composition
Eugenol
Mite lethality lead
Apis Mellifera
Varroa destructor
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The oil obtained by hydrodistillation of the floral bottom of Syzygium aromaticum (L.) Merr. et Perry was analyzed by GC and GC/MS. Eugenol was the main constituent in the oil (86.7%). The biological activity of the oil applied to Varroa destructor and Apis mellifera was evaluated in two laboratory tests. Mite lethality was estimated using a complete exposure method test with the oil at different concentrations, and a systemic administration method of oil at different concentrations diluted in syrup was placed in feeders for bees. The LC50 for complete exposure method at 24 h was 0.59 mL/dish. The inferior and superior limits obtained were 0.47 x 10-6 mL/dish and 1.22 mL/dish, respectively. LC50 estimated at 48 h showed a slight decrease as compared to that recorded at 24 h. Ratio selection (LC50 of A. mellifera/ LC50 of V. destructor) for complete exposure method was 26.46 and 13.35 for 24 h and 48 h, respectively. Regarding the systemic administration method, mites LC50 at 24 h was 12,300 ppm. The inferior and superior limits calculated were 9,214 ppm and 15,178 ppm, respectively. LC50 estimated at 48 h showed a slight decrease as compared to that recorded at 24 h. Ratio selection for systemic administration method was 3.05 and 2.22 for 24 h and 48 h, respectively. Syzygium aromaticum oil was found to be an attractant for V. destructor at 4.8% (w/w) concentration. The results showed that oil toxicity against V. destructor differed depending upon its administration. Nevertheless, the ratio selection calculated by this oil is expected to enable its application under field conditions with a good safety margin. This oil could also be used in combination with other oils in integrated pest management strategies in bee colonies.
Fil: Maggi, Matías Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología. Laboratorio de Artrópodos; Argentina
Fil: Ruffnengo, Sergio R.. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología. Laboratorio de Artrópodos; Argentina
Fil: Gende, Liesel Brenda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología. Laboratorio de Artrópodos; Argentina
Fil: Sarlo, Edgardo G.. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología. Laboratorio de Artrópodos; Argentina
Fil: Eguaras, Martin Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología. Laboratorio de Artrópodos; Argentina
Fil: Bailac, Pedro Nelson. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina
Fil: Ponzi, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina
description The oil obtained by hydrodistillation of the floral bottom of Syzygium aromaticum (L.) Merr. et Perry was analyzed by GC and GC/MS. Eugenol was the main constituent in the oil (86.7%). The biological activity of the oil applied to Varroa destructor and Apis mellifera was evaluated in two laboratory tests. Mite lethality was estimated using a complete exposure method test with the oil at different concentrations, and a systemic administration method of oil at different concentrations diluted in syrup was placed in feeders for bees. The LC50 for complete exposure method at 24 h was 0.59 mL/dish. The inferior and superior limits obtained were 0.47 x 10-6 mL/dish and 1.22 mL/dish, respectively. LC50 estimated at 48 h showed a slight decrease as compared to that recorded at 24 h. Ratio selection (LC50 of A. mellifera/ LC50 of V. destructor) for complete exposure method was 26.46 and 13.35 for 24 h and 48 h, respectively. Regarding the systemic administration method, mites LC50 at 24 h was 12,300 ppm. The inferior and superior limits calculated were 9,214 ppm and 15,178 ppm, respectively. LC50 estimated at 48 h showed a slight decrease as compared to that recorded at 24 h. Ratio selection for systemic administration method was 3.05 and 2.22 for 24 h and 48 h, respectively. Syzygium aromaticum oil was found to be an attractant for V. destructor at 4.8% (w/w) concentration. The results showed that oil toxicity against V. destructor differed depending upon its administration. Nevertheless, the ratio selection calculated by this oil is expected to enable its application under field conditions with a good safety margin. This oil could also be used in combination with other oils in integrated pest management strategies in bee colonies.
publishDate 2010
dc.date.none.fl_str_mv 2010-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/242407
Maggi, Matías Daniel; Ruffnengo, Sergio R.; Gende, Liesel Brenda; Sarlo, Edgardo G.; Eguaras, Martin Javier; et al.; Laboratory Evaluations of Syzygium aromaticum (L.) Merr. et Perry Essential Oil Against Varroa destructor; Allured Publishing Corporation; Journal of Essential Oil Research; 22; 2; 3-2010; 119-122
1041-2905
CONICET Digital
CONICET
url http://hdl.handle.net/11336/242407
identifier_str_mv Maggi, Matías Daniel; Ruffnengo, Sergio R.; Gende, Liesel Brenda; Sarlo, Edgardo G.; Eguaras, Martin Javier; et al.; Laboratory Evaluations of Syzygium aromaticum (L.) Merr. et Perry Essential Oil Against Varroa destructor; Allured Publishing Corporation; Journal of Essential Oil Research; 22; 2; 3-2010; 119-122
1041-2905
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/doi/10.1080/10412905.2010.9700278
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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dc.format.none.fl_str_mv application/pdf
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dc.publisher.none.fl_str_mv Allured Publishing Corporation
publisher.none.fl_str_mv Allured Publishing Corporation
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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