Oral squamous cell carcinoma (OSCC) tumors from heavy alcohol consumers are associated with higher levels of TLR9 and a particular immunophenotype: Impact on patient survival
- Autores
- Bolesina, Nicolás Jorge; Gatti, Gerardo Alberto; López de Blanc, Silvia; Dhooge, Sabrina; Rocha, Darío Gastón; Fernandez, Elmer Andres; Ferreyra, Ruth Salome; Palla, Vanesa; Grupe, Verónica Maria; Morelatto, Rosana Andrea; Maccioni, Mariana
- Año de publicación
- 2022
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Oral squamous cell carcinoma (OSCC) is one of the most frequent types of oral cancer in developing countries and its burden correlates with exposure to tobacco and excessive alcohol consumption. Toll like receptors (TLRs) are major sensors of inflammatory stimuli, from both microbial and sterile causes and as such, they have been related to tumor progression and metastasis. Here, we evaluated the expression of TLR2, 4 and 9 as well as CD3+, CD8+ and Granzyme B+ cell infiltration by immunohistochemistry in oral samples of 30 patients with OSCC, classified according to their consumption of alcohol. Our findings indicate that there is a significant association between heavy alcohol consumption and tumors with higher expression levels of TLR9. Moreover, patients with TLR9high tumors, as well as those who indicated high consumption of alcohol exhibited a diminished overall survival. TCGA data analysis indicated that TLR9high tumors express a significant increase in some genes related with the oral cavity itself, inflammation and tumor promotion. Our analysis of tumor infiltrating leukocytes demonstrated that the major differences perceived in heavy alcohol consumers was the location of CD8+ T cells infiltrating the tumor, which showed lower numbers intratumorally. Our data suggest the existence of a pathogenic loop that involves alcohol consumption, high TLR9 expression and the immunophenotype, which might have a profound impact on the progression of the disease.
Fil: Bolesina, Nicolás Jorge. Universidad Nacional de Córdoba; Argentina
Fil: Gatti, Gerardo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: López de Blanc, Silvia. Universidad Nacional de Córdoba; Argentina
Fil: Dhooge, Sabrina. Fundación Para El Progreso de la Medicina; Argentina
Fil: Rocha, Darío Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación y Desarrollo en Inmunología y Enfermedades Infecciosas. Universidad Católica de Córdoba. Centro de Investigación y Desarrollo en Inmunología y Enfermedades Infecciosas; Argentina
Fil: Fernandez, Elmer Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación y Desarrollo en Inmunología y Enfermedades Infecciosas. Universidad Católica de Córdoba. Centro de Investigación y Desarrollo en Inmunología y Enfermedades Infecciosas; Argentina
Fil: Ferreyra, Ruth Salome. Universidad Nacional de Córdoba; Argentina
Fil: Palla, Vanesa. Fundación Para El Progreso de la Medicina; Argentina
Fil: Grupe, Verónica Maria. Fundación Para El Progreso de la Medicina; Argentina
Fil: Morelatto, Rosana Andrea. Universidad Nacional de Córdoba; Argentina
Fil: Maccioni, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina - Materia
-
ALCOHOL CONSUMPTION
CD8+T CELL INFILTRATION
IMMUNOPHENOTYPE
ORAL SQUAMOUS CELL CARCINOMA
TLR9 - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/218194
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Oral squamous cell carcinoma (OSCC) tumors from heavy alcohol consumers are associated with higher levels of TLR9 and a particular immunophenotype: Impact on patient survivalBolesina, Nicolás JorgeGatti, Gerardo AlbertoLópez de Blanc, SilviaDhooge, SabrinaRocha, Darío GastónFernandez, Elmer AndresFerreyra, Ruth SalomePalla, VanesaGrupe, Verónica MariaMorelatto, Rosana AndreaMaccioni, MarianaALCOHOL CONSUMPTIONCD8+T CELL INFILTRATIONIMMUNOPHENOTYPEORAL SQUAMOUS CELL CARCINOMATLR9https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Oral squamous cell carcinoma (OSCC) is one of the most frequent types of oral cancer in developing countries and its burden correlates with exposure to tobacco and excessive alcohol consumption. Toll like receptors (TLRs) are major sensors of inflammatory stimuli, from both microbial and sterile causes and as such, they have been related to tumor progression and metastasis. Here, we evaluated the expression of TLR2, 4 and 9 as well as CD3+, CD8+ and Granzyme B+ cell infiltration by immunohistochemistry in oral samples of 30 patients with OSCC, classified according to their consumption of alcohol. Our findings indicate that there is a significant association between heavy alcohol consumption and tumors with higher expression levels of TLR9. Moreover, patients with TLR9high tumors, as well as those who indicated high consumption of alcohol exhibited a diminished overall survival. TCGA data analysis indicated that TLR9high tumors express a significant increase in some genes related with the oral cavity itself, inflammation and tumor promotion. Our analysis of tumor infiltrating leukocytes demonstrated that the major differences perceived in heavy alcohol consumers was the location of CD8+ T cells infiltrating the tumor, which showed lower numbers intratumorally. Our data suggest the existence of a pathogenic loop that involves alcohol consumption, high TLR9 expression and the immunophenotype, which might have a profound impact on the progression of the disease.Fil: Bolesina, Nicolás Jorge. Universidad Nacional de Córdoba; ArgentinaFil: Gatti, Gerardo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: López de Blanc, Silvia. Universidad Nacional de Córdoba; ArgentinaFil: Dhooge, Sabrina. Fundación Para El Progreso de la Medicina; ArgentinaFil: Rocha, Darío Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación y Desarrollo en Inmunología y Enfermedades Infecciosas. Universidad Católica de Córdoba. Centro de Investigación y Desarrollo en Inmunología y Enfermedades Infecciosas; ArgentinaFil: Fernandez, Elmer Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación y Desarrollo en Inmunología y Enfermedades Infecciosas. Universidad Católica de Córdoba. Centro de Investigación y Desarrollo en Inmunología y Enfermedades Infecciosas; ArgentinaFil: Ferreyra, Ruth Salome. Universidad Nacional de Córdoba; ArgentinaFil: Palla, Vanesa. Fundación Para El Progreso de la Medicina; ArgentinaFil: Grupe, Verónica Maria. Fundación Para El Progreso de la Medicina; ArgentinaFil: Morelatto, Rosana Andrea. Universidad Nacional de Córdoba; ArgentinaFil: Maccioni, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFrontiers Media2022-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/218194Bolesina, Nicolás Jorge; Gatti, Gerardo Alberto; López de Blanc, Silvia; Dhooge, Sabrina; Rocha, Darío Gastón; et al.; Oral squamous cell carcinoma (OSCC) tumors from heavy alcohol consumers are associated with higher levels of TLR9 and a particular immunophenotype: Impact on patient survival; Frontiers Media; Frontiers in Immunology; 13; 8-2022; 1-131664-3224CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fimmu.2022.941667/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fimmu.2022.941667info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:54:26Zoai:ri.conicet.gov.ar:11336/218194instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:54:27.261CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Oral squamous cell carcinoma (OSCC) tumors from heavy alcohol consumers are associated with higher levels of TLR9 and a particular immunophenotype: Impact on patient survival |
title |
Oral squamous cell carcinoma (OSCC) tumors from heavy alcohol consumers are associated with higher levels of TLR9 and a particular immunophenotype: Impact on patient survival |
spellingShingle |
Oral squamous cell carcinoma (OSCC) tumors from heavy alcohol consumers are associated with higher levels of TLR9 and a particular immunophenotype: Impact on patient survival Bolesina, Nicolás Jorge ALCOHOL CONSUMPTION CD8+T CELL INFILTRATION IMMUNOPHENOTYPE ORAL SQUAMOUS CELL CARCINOMA TLR9 |
title_short |
Oral squamous cell carcinoma (OSCC) tumors from heavy alcohol consumers are associated with higher levels of TLR9 and a particular immunophenotype: Impact on patient survival |
title_full |
Oral squamous cell carcinoma (OSCC) tumors from heavy alcohol consumers are associated with higher levels of TLR9 and a particular immunophenotype: Impact on patient survival |
title_fullStr |
Oral squamous cell carcinoma (OSCC) tumors from heavy alcohol consumers are associated with higher levels of TLR9 and a particular immunophenotype: Impact on patient survival |
title_full_unstemmed |
Oral squamous cell carcinoma (OSCC) tumors from heavy alcohol consumers are associated with higher levels of TLR9 and a particular immunophenotype: Impact on patient survival |
title_sort |
Oral squamous cell carcinoma (OSCC) tumors from heavy alcohol consumers are associated with higher levels of TLR9 and a particular immunophenotype: Impact on patient survival |
dc.creator.none.fl_str_mv |
Bolesina, Nicolás Jorge Gatti, Gerardo Alberto López de Blanc, Silvia Dhooge, Sabrina Rocha, Darío Gastón Fernandez, Elmer Andres Ferreyra, Ruth Salome Palla, Vanesa Grupe, Verónica Maria Morelatto, Rosana Andrea Maccioni, Mariana |
author |
Bolesina, Nicolás Jorge |
author_facet |
Bolesina, Nicolás Jorge Gatti, Gerardo Alberto López de Blanc, Silvia Dhooge, Sabrina Rocha, Darío Gastón Fernandez, Elmer Andres Ferreyra, Ruth Salome Palla, Vanesa Grupe, Verónica Maria Morelatto, Rosana Andrea Maccioni, Mariana |
author_role |
author |
author2 |
Gatti, Gerardo Alberto López de Blanc, Silvia Dhooge, Sabrina Rocha, Darío Gastón Fernandez, Elmer Andres Ferreyra, Ruth Salome Palla, Vanesa Grupe, Verónica Maria Morelatto, Rosana Andrea Maccioni, Mariana |
author2_role |
author author author author author author author author author author |
dc.subject.none.fl_str_mv |
ALCOHOL CONSUMPTION CD8+T CELL INFILTRATION IMMUNOPHENOTYPE ORAL SQUAMOUS CELL CARCINOMA TLR9 |
topic |
ALCOHOL CONSUMPTION CD8+T CELL INFILTRATION IMMUNOPHENOTYPE ORAL SQUAMOUS CELL CARCINOMA TLR9 |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Oral squamous cell carcinoma (OSCC) is one of the most frequent types of oral cancer in developing countries and its burden correlates with exposure to tobacco and excessive alcohol consumption. Toll like receptors (TLRs) are major sensors of inflammatory stimuli, from both microbial and sterile causes and as such, they have been related to tumor progression and metastasis. Here, we evaluated the expression of TLR2, 4 and 9 as well as CD3+, CD8+ and Granzyme B+ cell infiltration by immunohistochemistry in oral samples of 30 patients with OSCC, classified according to their consumption of alcohol. Our findings indicate that there is a significant association between heavy alcohol consumption and tumors with higher expression levels of TLR9. Moreover, patients with TLR9high tumors, as well as those who indicated high consumption of alcohol exhibited a diminished overall survival. TCGA data analysis indicated that TLR9high tumors express a significant increase in some genes related with the oral cavity itself, inflammation and tumor promotion. Our analysis of tumor infiltrating leukocytes demonstrated that the major differences perceived in heavy alcohol consumers was the location of CD8+ T cells infiltrating the tumor, which showed lower numbers intratumorally. Our data suggest the existence of a pathogenic loop that involves alcohol consumption, high TLR9 expression and the immunophenotype, which might have a profound impact on the progression of the disease. Fil: Bolesina, Nicolás Jorge. Universidad Nacional de Córdoba; Argentina Fil: Gatti, Gerardo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina Fil: López de Blanc, Silvia. Universidad Nacional de Córdoba; Argentina Fil: Dhooge, Sabrina. Fundación Para El Progreso de la Medicina; Argentina Fil: Rocha, Darío Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación y Desarrollo en Inmunología y Enfermedades Infecciosas. Universidad Católica de Córdoba. Centro de Investigación y Desarrollo en Inmunología y Enfermedades Infecciosas; Argentina Fil: Fernandez, Elmer Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación y Desarrollo en Inmunología y Enfermedades Infecciosas. Universidad Católica de Córdoba. Centro de Investigación y Desarrollo en Inmunología y Enfermedades Infecciosas; Argentina Fil: Ferreyra, Ruth Salome. Universidad Nacional de Córdoba; Argentina Fil: Palla, Vanesa. Fundación Para El Progreso de la Medicina; Argentina Fil: Grupe, Verónica Maria. Fundación Para El Progreso de la Medicina; Argentina Fil: Morelatto, Rosana Andrea. Universidad Nacional de Córdoba; Argentina Fil: Maccioni, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina |
description |
Oral squamous cell carcinoma (OSCC) is one of the most frequent types of oral cancer in developing countries and its burden correlates with exposure to tobacco and excessive alcohol consumption. Toll like receptors (TLRs) are major sensors of inflammatory stimuli, from both microbial and sterile causes and as such, they have been related to tumor progression and metastasis. Here, we evaluated the expression of TLR2, 4 and 9 as well as CD3+, CD8+ and Granzyme B+ cell infiltration by immunohistochemistry in oral samples of 30 patients with OSCC, classified according to their consumption of alcohol. Our findings indicate that there is a significant association between heavy alcohol consumption and tumors with higher expression levels of TLR9. Moreover, patients with TLR9high tumors, as well as those who indicated high consumption of alcohol exhibited a diminished overall survival. TCGA data analysis indicated that TLR9high tumors express a significant increase in some genes related with the oral cavity itself, inflammation and tumor promotion. Our analysis of tumor infiltrating leukocytes demonstrated that the major differences perceived in heavy alcohol consumers was the location of CD8+ T cells infiltrating the tumor, which showed lower numbers intratumorally. Our data suggest the existence of a pathogenic loop that involves alcohol consumption, high TLR9 expression and the immunophenotype, which might have a profound impact on the progression of the disease. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-08 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/218194 Bolesina, Nicolás Jorge; Gatti, Gerardo Alberto; López de Blanc, Silvia; Dhooge, Sabrina; Rocha, Darío Gastón; et al.; Oral squamous cell carcinoma (OSCC) tumors from heavy alcohol consumers are associated with higher levels of TLR9 and a particular immunophenotype: Impact on patient survival; Frontiers Media; Frontiers in Immunology; 13; 8-2022; 1-13 1664-3224 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/218194 |
identifier_str_mv |
Bolesina, Nicolás Jorge; Gatti, Gerardo Alberto; López de Blanc, Silvia; Dhooge, Sabrina; Rocha, Darío Gastón; et al.; Oral squamous cell carcinoma (OSCC) tumors from heavy alcohol consumers are associated with higher levels of TLR9 and a particular immunophenotype: Impact on patient survival; Frontiers Media; Frontiers in Immunology; 13; 8-2022; 1-13 1664-3224 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fimmu.2022.941667/full info:eu-repo/semantics/altIdentifier/doi/10.3389/fimmu.2022.941667 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Frontiers Media |
publisher.none.fl_str_mv |
Frontiers Media |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |