Oral squamous cell carcinoma (OSCC) tumors from heavy alcohol consumers are associated with higher levels of TLR9 and a particular immunophenotype: Impact on patient survival

Autores
Bolesina, Nicolás Jorge; Gatti, Gerardo Alberto; López de Blanc, Silvia; Dhooge, Sabrina; Rocha, Darío Gastón; Fernandez, Elmer Andres; Ferreyra, Ruth Salome; Palla, Vanesa; Grupe, Verónica Maria; Morelatto, Rosana Andrea; Maccioni, Mariana
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Oral squamous cell carcinoma (OSCC) is one of the most frequent types of oral cancer in developing countries and its burden correlates with exposure to tobacco and excessive alcohol consumption. Toll like receptors (TLRs) are major sensors of inflammatory stimuli, from both microbial and sterile causes and as such, they have been related to tumor progression and metastasis. Here, we evaluated the expression of TLR2, 4 and 9 as well as CD3+, CD8+ and Granzyme B+ cell infiltration by immunohistochemistry in oral samples of 30 patients with OSCC, classified according to their consumption of alcohol. Our findings indicate that there is a significant association between heavy alcohol consumption and tumors with higher expression levels of TLR9. Moreover, patients with TLR9high tumors, as well as those who indicated high consumption of alcohol exhibited a diminished overall survival. TCGA data analysis indicated that TLR9high tumors express a significant increase in some genes related with the oral cavity itself, inflammation and tumor promotion. Our analysis of tumor infiltrating leukocytes demonstrated that the major differences perceived in heavy alcohol consumers was the location of CD8+ T cells infiltrating the tumor, which showed lower numbers intratumorally. Our data suggest the existence of a pathogenic loop that involves alcohol consumption, high TLR9 expression and the immunophenotype, which might have a profound impact on the progression of the disease.
Fil: Bolesina, Nicolás Jorge. Universidad Nacional de Córdoba; Argentina
Fil: Gatti, Gerardo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: López de Blanc, Silvia. Universidad Nacional de Córdoba; Argentina
Fil: Dhooge, Sabrina. Fundación Para El Progreso de la Medicina; Argentina
Fil: Rocha, Darío Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación y Desarrollo en Inmunología y Enfermedades Infecciosas. Universidad Católica de Córdoba. Centro de Investigación y Desarrollo en Inmunología y Enfermedades Infecciosas; Argentina
Fil: Fernandez, Elmer Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación y Desarrollo en Inmunología y Enfermedades Infecciosas. Universidad Católica de Córdoba. Centro de Investigación y Desarrollo en Inmunología y Enfermedades Infecciosas; Argentina
Fil: Ferreyra, Ruth Salome. Universidad Nacional de Córdoba; Argentina
Fil: Palla, Vanesa. Fundación Para El Progreso de la Medicina; Argentina
Fil: Grupe, Verónica Maria. Fundación Para El Progreso de la Medicina; Argentina
Fil: Morelatto, Rosana Andrea. Universidad Nacional de Córdoba; Argentina
Fil: Maccioni, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Materia
ALCOHOL CONSUMPTION
CD8+T CELL INFILTRATION
IMMUNOPHENOTYPE
ORAL SQUAMOUS CELL CARCINOMA
TLR9
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/218194
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/218194
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Oral squamous cell carcinoma (OSCC) tumors from heavy alcohol consumers are associated with higher levels of TLR9 and a particular immunophenotype: Impact on patient survivalBolesina, Nicolás JorgeGatti, Gerardo AlbertoLópez de Blanc, SilviaDhooge, SabrinaRocha, Darío GastónFernandez, Elmer AndresFerreyra, Ruth SalomePalla, VanesaGrupe, Verónica MariaMorelatto, Rosana AndreaMaccioni, MarianaALCOHOL CONSUMPTIONCD8+T CELL INFILTRATIONIMMUNOPHENOTYPEORAL SQUAMOUS CELL CARCINOMATLR9https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Oral squamous cell carcinoma (OSCC) is one of the most frequent types of oral cancer in developing countries and its burden correlates with exposure to tobacco and excessive alcohol consumption. Toll like receptors (TLRs) are major sensors of inflammatory stimuli, from both microbial and sterile causes and as such, they have been related to tumor progression and metastasis. Here, we evaluated the expression of TLR2, 4 and 9 as well as CD3+, CD8+ and Granzyme B+ cell infiltration by immunohistochemistry in oral samples of 30 patients with OSCC, classified according to their consumption of alcohol. Our findings indicate that there is a significant association between heavy alcohol consumption and tumors with higher expression levels of TLR9. Moreover, patients with TLR9high tumors, as well as those who indicated high consumption of alcohol exhibited a diminished overall survival. TCGA data analysis indicated that TLR9high tumors express a significant increase in some genes related with the oral cavity itself, inflammation and tumor promotion. Our analysis of tumor infiltrating leukocytes demonstrated that the major differences perceived in heavy alcohol consumers was the location of CD8+ T cells infiltrating the tumor, which showed lower numbers intratumorally. Our data suggest the existence of a pathogenic loop that involves alcohol consumption, high TLR9 expression and the immunophenotype, which might have a profound impact on the progression of the disease.Fil: Bolesina, Nicolás Jorge. Universidad Nacional de Córdoba; ArgentinaFil: Gatti, Gerardo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: López de Blanc, Silvia. Universidad Nacional de Córdoba; ArgentinaFil: Dhooge, Sabrina. Fundación Para El Progreso de la Medicina; ArgentinaFil: Rocha, Darío Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación y Desarrollo en Inmunología y Enfermedades Infecciosas. Universidad Católica de Córdoba. Centro de Investigación y Desarrollo en Inmunología y Enfermedades Infecciosas; ArgentinaFil: Fernandez, Elmer Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación y Desarrollo en Inmunología y Enfermedades Infecciosas. Universidad Católica de Córdoba. Centro de Investigación y Desarrollo en Inmunología y Enfermedades Infecciosas; ArgentinaFil: Ferreyra, Ruth Salome. Universidad Nacional de Córdoba; ArgentinaFil: Palla, Vanesa. Fundación Para El Progreso de la Medicina; ArgentinaFil: Grupe, Verónica Maria. Fundación Para El Progreso de la Medicina; ArgentinaFil: Morelatto, Rosana Andrea. Universidad Nacional de Córdoba; ArgentinaFil: Maccioni, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFrontiers Media2022-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/218194Bolesina, Nicolás Jorge; Gatti, Gerardo Alberto; López de Blanc, Silvia; Dhooge, Sabrina; Rocha, Darío Gastón; et al.; Oral squamous cell carcinoma (OSCC) tumors from heavy alcohol consumers are associated with higher levels of TLR9 and a particular immunophenotype: Impact on patient survival; Frontiers Media; Frontiers in Immunology; 13; 8-2022; 1-131664-3224CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fimmu.2022.941667/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fimmu.2022.941667info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:23:45Zoai:ri.conicet.gov.ar:11336/218194instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:23:45.362CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Oral squamous cell carcinoma (OSCC) tumors from heavy alcohol consumers are associated with higher levels of TLR9 and a particular immunophenotype: Impact on patient survival
title Oral squamous cell carcinoma (OSCC) tumors from heavy alcohol consumers are associated with higher levels of TLR9 and a particular immunophenotype: Impact on patient survival
spellingShingle Oral squamous cell carcinoma (OSCC) tumors from heavy alcohol consumers are associated with higher levels of TLR9 and a particular immunophenotype: Impact on patient survival
Bolesina, Nicolás Jorge
ALCOHOL CONSUMPTION
CD8+T CELL INFILTRATION
IMMUNOPHENOTYPE
ORAL SQUAMOUS CELL CARCINOMA
TLR9
title_short Oral squamous cell carcinoma (OSCC) tumors from heavy alcohol consumers are associated with higher levels of TLR9 and a particular immunophenotype: Impact on patient survival
title_full Oral squamous cell carcinoma (OSCC) tumors from heavy alcohol consumers are associated with higher levels of TLR9 and a particular immunophenotype: Impact on patient survival
title_fullStr Oral squamous cell carcinoma (OSCC) tumors from heavy alcohol consumers are associated with higher levels of TLR9 and a particular immunophenotype: Impact on patient survival
title_full_unstemmed Oral squamous cell carcinoma (OSCC) tumors from heavy alcohol consumers are associated with higher levels of TLR9 and a particular immunophenotype: Impact on patient survival
title_sort Oral squamous cell carcinoma (OSCC) tumors from heavy alcohol consumers are associated with higher levels of TLR9 and a particular immunophenotype: Impact on patient survival
dc.creator.none.fl_str_mv Bolesina, Nicolás Jorge
Gatti, Gerardo Alberto
López de Blanc, Silvia
Dhooge, Sabrina
Rocha, Darío Gastón
Fernandez, Elmer Andres
Ferreyra, Ruth Salome
Palla, Vanesa
Grupe, Verónica Maria
Morelatto, Rosana Andrea
Maccioni, Mariana
author Bolesina, Nicolás Jorge
author_facet Bolesina, Nicolás Jorge
Gatti, Gerardo Alberto
López de Blanc, Silvia
Dhooge, Sabrina
Rocha, Darío Gastón
Fernandez, Elmer Andres
Ferreyra, Ruth Salome
Palla, Vanesa
Grupe, Verónica Maria
Morelatto, Rosana Andrea
Maccioni, Mariana
author_role author
author2 Gatti, Gerardo Alberto
López de Blanc, Silvia
Dhooge, Sabrina
Rocha, Darío Gastón
Fernandez, Elmer Andres
Ferreyra, Ruth Salome
Palla, Vanesa
Grupe, Verónica Maria
Morelatto, Rosana Andrea
Maccioni, Mariana
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ALCOHOL CONSUMPTION
CD8+T CELL INFILTRATION
IMMUNOPHENOTYPE
ORAL SQUAMOUS CELL CARCINOMA
TLR9
topic ALCOHOL CONSUMPTION
CD8+T CELL INFILTRATION
IMMUNOPHENOTYPE
ORAL SQUAMOUS CELL CARCINOMA
TLR9
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Oral squamous cell carcinoma (OSCC) is one of the most frequent types of oral cancer in developing countries and its burden correlates with exposure to tobacco and excessive alcohol consumption. Toll like receptors (TLRs) are major sensors of inflammatory stimuli, from both microbial and sterile causes and as such, they have been related to tumor progression and metastasis. Here, we evaluated the expression of TLR2, 4 and 9 as well as CD3+, CD8+ and Granzyme B+ cell infiltration by immunohistochemistry in oral samples of 30 patients with OSCC, classified according to their consumption of alcohol. Our findings indicate that there is a significant association between heavy alcohol consumption and tumors with higher expression levels of TLR9. Moreover, patients with TLR9high tumors, as well as those who indicated high consumption of alcohol exhibited a diminished overall survival. TCGA data analysis indicated that TLR9high tumors express a significant increase in some genes related with the oral cavity itself, inflammation and tumor promotion. Our analysis of tumor infiltrating leukocytes demonstrated that the major differences perceived in heavy alcohol consumers was the location of CD8+ T cells infiltrating the tumor, which showed lower numbers intratumorally. Our data suggest the existence of a pathogenic loop that involves alcohol consumption, high TLR9 expression and the immunophenotype, which might have a profound impact on the progression of the disease.
Fil: Bolesina, Nicolás Jorge. Universidad Nacional de Córdoba; Argentina
Fil: Gatti, Gerardo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: López de Blanc, Silvia. Universidad Nacional de Córdoba; Argentina
Fil: Dhooge, Sabrina. Fundación Para El Progreso de la Medicina; Argentina
Fil: Rocha, Darío Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación y Desarrollo en Inmunología y Enfermedades Infecciosas. Universidad Católica de Córdoba. Centro de Investigación y Desarrollo en Inmunología y Enfermedades Infecciosas; Argentina
Fil: Fernandez, Elmer Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación y Desarrollo en Inmunología y Enfermedades Infecciosas. Universidad Católica de Córdoba. Centro de Investigación y Desarrollo en Inmunología y Enfermedades Infecciosas; Argentina
Fil: Ferreyra, Ruth Salome. Universidad Nacional de Córdoba; Argentina
Fil: Palla, Vanesa. Fundación Para El Progreso de la Medicina; Argentina
Fil: Grupe, Verónica Maria. Fundación Para El Progreso de la Medicina; Argentina
Fil: Morelatto, Rosana Andrea. Universidad Nacional de Córdoba; Argentina
Fil: Maccioni, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
description Oral squamous cell carcinoma (OSCC) is one of the most frequent types of oral cancer in developing countries and its burden correlates with exposure to tobacco and excessive alcohol consumption. Toll like receptors (TLRs) are major sensors of inflammatory stimuli, from both microbial and sterile causes and as such, they have been related to tumor progression and metastasis. Here, we evaluated the expression of TLR2, 4 and 9 as well as CD3+, CD8+ and Granzyme B+ cell infiltration by immunohistochemistry in oral samples of 30 patients with OSCC, classified according to their consumption of alcohol. Our findings indicate that there is a significant association between heavy alcohol consumption and tumors with higher expression levels of TLR9. Moreover, patients with TLR9high tumors, as well as those who indicated high consumption of alcohol exhibited a diminished overall survival. TCGA data analysis indicated that TLR9high tumors express a significant increase in some genes related with the oral cavity itself, inflammation and tumor promotion. Our analysis of tumor infiltrating leukocytes demonstrated that the major differences perceived in heavy alcohol consumers was the location of CD8+ T cells infiltrating the tumor, which showed lower numbers intratumorally. Our data suggest the existence of a pathogenic loop that involves alcohol consumption, high TLR9 expression and the immunophenotype, which might have a profound impact on the progression of the disease.
publishDate 2022
dc.date.none.fl_str_mv 2022-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/218194
Bolesina, Nicolás Jorge; Gatti, Gerardo Alberto; López de Blanc, Silvia; Dhooge, Sabrina; Rocha, Darío Gastón; et al.; Oral squamous cell carcinoma (OSCC) tumors from heavy alcohol consumers are associated with higher levels of TLR9 and a particular immunophenotype: Impact on patient survival; Frontiers Media; Frontiers in Immunology; 13; 8-2022; 1-13
1664-3224
CONICET Digital
CONICET
url http://hdl.handle.net/11336/218194
identifier_str_mv Bolesina, Nicolás Jorge; Gatti, Gerardo Alberto; López de Blanc, Silvia; Dhooge, Sabrina; Rocha, Darío Gastón; et al.; Oral squamous cell carcinoma (OSCC) tumors from heavy alcohol consumers are associated with higher levels of TLR9 and a particular immunophenotype: Impact on patient survival; Frontiers Media; Frontiers in Immunology; 13; 8-2022; 1-13
1664-3224
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fimmu.2022.941667/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fimmu.2022.941667
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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